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Q9NXL9 (MCM9_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA helicase MCM9

Short name=hMCM9
EC=3.6.4.12
Alternative name(s):
Mini-chromosome maintenance deficient domain-containing protein 1
Minichromosome maintenance 9
Gene names
Name:MCM9
Synonyms:C6orf61, MCMDC1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1143 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the MCM8-MCM9 complex, a complex involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. The MCM8-MCM9 complex probably acts as a hexameric helicase downstream of the Fanconi anemia proteins BRCA2 and RAD51 and is required to process aberrant forks into homologous recombination substrates and to orchestrate homologous recombination with resection, fork stabilization and fork restart. Ref.7

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Component of the MCM8-MCM9 complex, which forms a hexamer composed of MCM8 and MCM9. Ref.7

Subcellular location

Nucleus By similarity. Note: Localizes to nuclear foci and colocalizes with RAD51 By similarity.

Developmental stage

The expression of isoform L and isoform M is cell cycle regulated: induced in S-phase, decreases through G2/M, and becomes constant through G1. Ref.8

Sequence similarities

Belongs to the MCM family.

Contains 1 MCM domain.

Sequence caution

The sequence BAA90991.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAG61142.1 differs from that shown. Reason: Erroneous termination at position 1070. Translated as Lys.

The sequence CAX30832.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform L (identifier: Q9NXL9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Most abundant isoform.
Isoform M (identifier: Q9NXL9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     606-648: GGALLGGVNA...LELQSLLSEE → VTESECAPIP...QNFITAGNQL
     649-1143: Missing.
Isoform S (identifier: Q9NXL9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     383-391: AGLTVTAVK → AGIVCDNFK
     392-1143: Missing.
Isoform 4 (identifier: Q9NXL9-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-381: Missing.
     382-383: SA → MS
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11431143DNA helicase MCM9
PRO_0000089513

Regions

Domain300 – 505206MCM
Nucleotide binding352 – 3598ATP Potential

Amino acid modifications

Modified residue7621Phosphoserine Ref.6

Natural variations

Alternative sequence1 – 381381Missing in isoform 4.
VSP_044180
Alternative sequence382 – 3832SA → MS in isoform 4.
VSP_044181
Alternative sequence383 – 3919AGLTVTAVK → AGIVCDNFK in isoform S.
VSP_028013
Alternative sequence392 – 1143752Missing in isoform S.
VSP_028014
Alternative sequence606 – 64843GGALL…LLSEE → VTESECAPIPTTGIGGRDYS RILEKWSRGRIKLQNFITAG NQL in isoform M.
VSP_047462
Alternative sequence649 – 1143495Missing in isoform M.
VSP_047463

Experimental info

Sequence conflict4331Q → R in BAG61142. Ref.1
Sequence conflict5581C → S in BAG61142. Ref.1
Sequence conflict10631F → V in BAA90991. Ref.1
Sequence conflict11361D → G in BAA90991. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform L [UniParc].

Last modified May 16, 2012. Version 4.
Checksum: 3C35C8B3476A470F

FASTA1,143127,313
        10         20         30         40         50         60 
MNSDQVTLVG QVFESYVSEY HKNDILLILK ERDEDAHYPV VVNAMTLFET NMEIGEYFNM 

        70         80         90        100        110        120 
FPSEVLTIFD SALRRSALTI LQSLSQPEAV SMKQNLHARI SGLPVCPELV REHIPKTKDV 

       130        140        150        160        170        180 
GHFLSVTGTV IRTSLVKVLE FERDYMCNKC KHVFVIKADF EQYYTFCRPS SCPSLESCDS 

       190        200        210        220        230        240 
SKFTCLSGLS SSPTRCRDYQ EIKIQEQVQR LSVGSIPRSM KVILEDDLVD SCKSGDDLTI 

       250        260        270        280        290        300 
YGIVMQRWKP FQQDVRCEVE IVLKANYIQV NNEQSSGIIM DEEVQKEFED FWEYYKSDPF 

       310        320        330        340        350        360 
AGRNVILASL CPQVFGMYLV KLAVAMVLAG GIQRTDATGT RVRGESHLLL VGDPGTGKSQ 

       370        380        390        400        410        420 
FLKYAAKITP RSVLTTGIGS TSAGLTVTAV KDSGEWNLEA GALVLADAGL CCIDEFNSLK 

       430        440        450        460        470        480 
EHDRTSIHEA MEQQTISVAK AGLVCKLNTR TTILAATNPK GQYDPQESVS VNIALGSPLL 

       490        500        510        520        530        540 
SRFDLILVLL DTKNEDWDRI ISSFILENKG YPSKSEKLWS MEKMKTYFCL IRNLQPTLSD 

       550        560        570        580        590        600 
VGNQVLLRYY QMQRQSDCRN AARTTIRLLE SLIRLAEAHA RLMFRDTVTL EDAITVVSVM 

       610        620        630        640        650        660 
ESSMQGGALL GGVNALHTSF PENPGEQYQR QCELILEKLE LQSLLSEELR RLERLQNQSV 

       670        680        690        700        710        720 
HQSQPRVLEV ETTPGSLRNG PGEESNFRTS SQQEINYSTH IFSPGGSPEG SPVLDPPPHL 

       730        740        750        760        770        780 
EPNRSTSRKH SAQHKNNRDD SLDWFDFMAT HQSEPKNTVV VSPHPKTSGE NMASKISNST 

       790        800        810        820        830        840 
SQGKEKSEPG QRSKVDIGLL PSPGETGVPW RADNVESNKK KRLALDSEAA VSADKPDSVL 

       850        860        870        880        890        900 
THHVPRNLQK LCKERAQKLC RNSTRVPAQC TVPSHPQSTP VHSPDRMLDS PKRKRPKSLA 

       910        920        930        940        950        960 
QVEEPAIENV KPPGSPVAKL AKFTFKQKSK LIHSFEDHSH VSPGATKIAV HSPKISQRRT 

       970        980        990       1000       1010       1020 
RRDAALPVKR PGKLTSTPGN QISSQPQGET KEVSQQPPEK HGPREKVMCA PEKRIIQPEL 

      1030       1040       1050       1060       1070       1080 
ELGNETGCAH LTCEGDKKEE VSGSNKSGKV HACTLARLAN FCFTPPSESK SKSPPPERKN 

      1090       1100       1110       1120       1130       1140 
RGERGPSSPP TTTAPMRVSK RKSFQLRGST EKLIVSKESL FTLPELGDEA FDCDWDEEMR 


KKS 

« Hide

Isoform M [UniParc].

Checksum: 344DC7E1E4BB7C40
Show »

FASTA64872,737
Isoform S [UniParc].

Checksum: 1AB5A6CFBCF4E40C
Show »

FASTA39143,983
Isoform 4 [UniParc].

Checksum: 2C3190DA9ACCA977
Show »

FASTA76284,442

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 865-1143 (ISOFORM L).
Tissue: Teratocarcinoma.
[2]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM S).
Tissue: Brain.
[4]"Identification of full genes and proteins of MCM9, a novel, vertebrate-specific member of the MCM2-8 protein family."
Lutzmann M., Maiorano D., Mechali M.
Gene 362:51-56(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[5]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[6]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-762, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Mcm8 and Mcm9 form a complex that functions in homologous recombination repair induced by DNA interstrand crosslinks."
Nishimura K., Ishiai M., Horikawa K., Fukagawa T., Takata M., Takisawa H., Kanemaki M.T.
Mol. Cell 47:511-522(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN THE MCM8-MCM9 COMPLEX.
[8]"Identification, quantification, and evolutionary analysis of a novel isoform of MCM9."
Jeffries E.P., Denq W.I., Bartko J.C., Trakselis M.A.
Gene 519:41-49(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE SPLICING (ISOFORM M), DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK299076 mRNA. Translation: BAG61142.1. Sequence problems.
AK000177 mRNA. Translation: BAA90991.1. Different initiation.
AL132874, AL359634 Genomic DNA. Translation: CAX30832.1. Sequence problems.
BC031658 mRNA. Translation: AAH31658.1.
BN000882 mRNA. Translation: CAJ70648.1.
CCDSCCDS5121.1. [Q9NXL9-3]
CCDS56447.1. [Q9NXL9-1]
RefSeqNP_060166.2. NM_017696.2. [Q9NXL9-1]
NP_694987.1. NM_153255.4. [Q9NXL9-3]
UniGeneHs.279008.
Hs.733116.
Hs.736853.

3D structure databases

ProteinModelPortalQ9NXL9.
SMRQ9NXL9. Positions 82-271, 347-605.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid129033. 2 interactions.
IntActQ9NXL9. 1 interaction.
STRING9606.ENSP00000312870.

PTM databases

PhosphoSiteQ9NXL9.

Polymorphism databases

DMDM387912921.

Proteomic databases

MaxQBQ9NXL9.
PaxDbQ9NXL9.
PRIDEQ9NXL9.

Protocols and materials databases

DNASU254394.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000316068; ENSP00000312870; ENSG00000111877. [Q9NXL9-3]
ENST00000316316; ENSP00000314505; ENSG00000111877. [Q9NXL9-1]
GeneID254394.
KEGGhsa:254394.
UCSCuc003pyh.3. human. [Q9NXL9-2]
uc021zeh.1. human. [Q9NXL9-1]

Organism-specific databases

CTD254394.
GeneCardsGC06M119136.
HGNCHGNC:21484. MCM9.
HPAHPA031137.
MIM610098. gene.
neXtProtNX_Q9NXL9.
PharmGKBPA162395071.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1241.
HOGENOMHOG000169812.
HOVERGENHBG108122.
InParanoidQ9NXL9.
KOK10738.
OMAEDFWEHY.
OrthoDBEOG7TQV0G.
PhylomeDBQ9NXL9.
TreeFamTF329421.

Gene expression databases

ArrayExpressQ9NXL9.
BgeeQ9NXL9.
CleanExHS_MCM9.
GenevestigatorQ9NXL9.

Family and domain databases

Gene3D2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProIPR003593. AAA+_ATPase.
IPR001208. MCM_DNA-dep_ATPase.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00493. MCM. 1 hit.
[Graphical view]
PRINTSPR01657. MCMFAMILY.
SMARTSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEPS50051. MCM_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi254394.
NextBio92339.
PROQ9NXL9.
SOURCESearch...

Entry information

Entry nameMCM9_HUMAN
AccessionPrimary (citable) accession number: Q9NXL9
Secondary accession number(s): B4DR30 expand/collapse secondary AC list , B9DI77, Q2KHJ0, Q8N5S5, Q9HCV5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: May 16, 2012
Last modified: July 9, 2014
This is version 94 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM