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Protein

tRNA (guanine(26)-N(2))-dimethyltransferase

Gene

TRMT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Dimethylates a single guanine residue at position 26 of most tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.

Catalytic activityi

2 S-adenosyl-L-methionine + guanine(26) in tRNA = 2 S-adenosyl-L-homocysteine + N2-dimethylguanine(26) in tRNA.PROSITE-ProRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri600 – 627C3H1-typePROSITE-ProRule annotationAdd BLAST28

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandMetal-binding, S-adenosyl-L-methionine, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-6782315 tRNA modification in the nucleus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine(26)-N(2))-dimethyltransferase (EC:2.1.1.216)
Alternative name(s):
tRNA 2,2-dimethylguanosine-26 methyltransferase
tRNA(guanine-26,N(2)-N(2)) methyltransferase
tRNA(m(2,2)G26)dimethyltransferase
Gene namesi
Name:TRMT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000104907.12
HGNCiHGNC:25980 TRMT1
MIMi611669 gene
neXtProtiNX_Q9NXH9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi55621
OpenTargetsiENSG00000104907
PharmGKBiPA134867808

Polymorphism and mutation databases

BioMutaiTRMT1
DMDMi12643821

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001476711 – 659tRNA (guanine(26)-N(2))-dimethyltransferaseAdd BLAST659

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei120PhosphoserineBy similarity1
Modified residuei517PhosphoserineCombined sources1
Modified residuei625PhosphoserineCombined sources1
Modified residuei628PhosphothreonineCombined sources1
Modified residuei646PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9NXH9
MaxQBiQ9NXH9
PaxDbiQ9NXH9
PeptideAtlasiQ9NXH9
PRIDEiQ9NXH9

PTM databases

iPTMnetiQ9NXH9
PhosphoSitePlusiQ9NXH9

Expressioni

Gene expression databases

BgeeiENSG00000104907
CleanExiHS_TRMT1
ExpressionAtlasiQ9NXH9 baseline and differential
GenevisibleiQ9NXH9 HS

Organism-specific databases

HPAiHPA041130
HPA041380

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi120760, 62 interactors
IntActiQ9NXH9, 3 interactors
MINTiQ9NXH9
STRINGi9606.ENSP00000350352

Structurei

3D structure databases

ProteinModelPortaliQ9NXH9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 499Trm1 methyltransferasePROSITE-ProRule annotationAdd BLAST445

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri600 – 627C3H1-typePROSITE-ProRule annotationAdd BLAST28

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1253 Eukaryota
COG1867 LUCA
GeneTreeiENSGT00530000063646
HOGENOMiHOG000177995
HOVERGENiHBG000477
InParanoidiQ9NXH9
KOiK00555
OMAiMRCWEKE
OrthoDBiEOG091G09UB
PhylomeDBiQ9NXH9
TreeFamiTF300851

Family and domain databases

InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR002905 Trm1
IPR000571 Znf_CCCH
IPR036855 Znf_CCCH_sf
PANTHERiPTHR10631 PTHR10631, 1 hit
PfamiView protein in Pfam
PF02005 TRM, 1 hit
PF00642 zf-CCCH, 1 hit
SMARTiView protein in SMART
SM00356 ZnF_C3H1, 1 hit
SUPFAMiSSF53335 SSF53335, 3 hits
SSF90229 SSF90229, 1 hit
TIGRFAMsiTIGR00308 TRM1, 1 hit
PROSITEiView protein in PROSITE
PS51626 SAM_MT_TRM1, 1 hit
PS50103 ZF_C3H1, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NXH9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQGSSLWLSL TFRSARVLSR ARFFEWQSPG LPNTAAMENG TGPYGEERPR
60 70 80 90 100
EVQETTVTEG AAKIAFPSAN EVFYNPVQEF NRDLTCAVIT EFARIQLGAK
110 120 130 140 150
GIQIKVPGEK DTQKVVVDLS EQEEEKVELK ESENLASGDQ PRTAAVGEIC
160 170 180 190 200
EEGLHVLEGL AASGLRSIRF ALEVPGLRSV VANDASTRAV DLIRRNVQLN
210 220 230 240 250
DVAHLVQPSQ ADARMLMYQH QRVSERFDVI DLDPYGSPAT FLDAAVQAVS
260 270 280 290 300
EGGLLCVTCT DMAVLAGNSG ETCYSKYGAM ALKSRACHEM ALRIVLHSLD
310 320 330 340 350
LRANCYQRFV VPLLSISADF YVRVFVRVFT GQAKVKASAS KQALVFQCVG
360 370 380 390 400
CGAFHLQRLG KASGVPSGRA KFSAACGPPV TPECEHCGQR HQLGGPMWAE
410 420 430 440 450
PIHDLDFVGR VLEAVSANPG RFHTSERIRG VLSVITEELP DVPLYYTLDQ
460 470 480 490 500
LSSTIHCNTP SLLQLRSALL HADFRVSLSH ACKNAVKTDA PASALWDIMR
510 520 530 540 550
CWEKECPVKR ERLSETSPAF RILSVEPRLQ ANFTIREDAN PSSRQRGLKR
560 570 580 590 600
FQANPEANWG PRPRARPGGK AADEAMEERR RLLQNKRKEP PEDVAQRAAR
610 620 630 640 650
LKTFPCKRFK EGTCQRGDQC CYSHSPPTPR VSADAAPDCP ETSNQTPPGP

GAAAGPGID
Length:659
Mass (Da):72,234
Last modified:October 1, 2000 - v1
Checksum:iE4F0F2B740B44387
GO
Isoform 2 (identifier: Q9NXH9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     340-368: Missing.

Note: No experimental confirmation available.
Show »
Length:630
Mass (Da):69,306
Checksum:i2CE1AF04EED60391
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti500 – 503RCWE → Q in AAC33150 (PubMed:15057824).Curated4

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016720340 – 368Missing in isoform 2. 1 PublicationAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF196479 mRNA Translation: AAG28495.1
AK000251 mRNA Translation: BAA91031.1
AC005546 Genomic DNA Translation: AAC33150.1
BC002492 mRNA Translation: AAH02492.1
BC018302 mRNA Translation: AAH18302.1
BC040126 mRNA Translation: AAH40126.1
CCDSiCCDS12293.1 [Q9NXH9-1]
CCDS45997.1 [Q9NXH9-2]
RefSeqiNP_001129507.1, NM_001136035.2 [Q9NXH9-1]
NP_001136026.1, NM_001142554.1 [Q9NXH9-2]
NP_060192.1, NM_017722.3 [Q9NXH9-1]
XP_016882433.1, XM_017026944.1 [Q9NXH9-1]
XP_016882434.1, XM_017026945.1 [Q9NXH9-2]
UniGeneiHs.515169

Genome annotation databases

EnsembliENST00000221504; ENSP00000221504; ENSG00000104907 [Q9NXH9-2]
ENST00000357720; ENSP00000350352; ENSG00000104907 [Q9NXH9-1]
ENST00000437766; ENSP00000416149; ENSG00000104907 [Q9NXH9-1]
ENST00000592062; ENSP00000466967; ENSG00000104907 [Q9NXH9-1]
GeneIDi55621
KEGGihsa:55621
UCSCiuc002mwj.3 human [Q9NXH9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTRM1_HUMAN
AccessioniPrimary (citable) accession number: Q9NXH9
Secondary accession number(s): O76103, Q548Y5, Q8WVA6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: October 1, 2000
Last modified: March 28, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health