Reviewed, UniProtKB/Swiss-Prot Q9NXH9 (TRM1_HUMAN)

Names and origin

Protein names

Recommended name:
    N(2),N(2)-dimethylguanosine tRNA methyltransferase
    EC=2.1.1.32
Alternative name(s):
    tRNA(guanine-26,N(2)-N(2)) methyltransferase
    tRNA 2,2-dimethylguanosine-26 methyltransferase
    tRNA(m(2,2)G26)dimethyltransferase

Gene names

Name:

TRMT1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	659 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Dimethylates a single guanine residue at position 26 of most tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(2)-methylguanine.
Sequence similarities	Contains 1 C3H1-type zinc finger.

Ontologies

Keywords
Biological process	tRNA processing
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	Metal-binding S-adenosyl-L-methionine Zinc
Molecular function	Methyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NXH9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NXH9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     340-368: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 659	659	N(2),N(2)-dimethylguanosine tRNA methyltransferase		PRO_0000147671
Regions
	Zinc finger	600 – 627	28	C3H1-type
Amino acid modifications
	Modified residue	120	1	Phosphoserine By similarity
	Modified residue	625	1	Phosphoserine Ref.7
	Modified residue	628	1	Phosphothreonine Ref.7 Ref.6
	Modified residue	646	1	Phosphothreonine Ref.7 Ref.5
Natural variations
	Alternative sequence	340 – 368	29	Missing in isoform 2.		VSP_016720
Experimental info
	Sequence conflict	500 – 503	4	RCWE → Q in AAC33150. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: E4F0F2B740B44387	FASTA	659	72,234
	10 20 30 40 50 60 MQGSSLWLSL TFRSARVLSR ARFFEWQSPG LPNTAAMENG TGPYGEERPR EVQETTVTEG 70 80 90 100 110 120 AAKIAFPSAN EVFYNPVQEF NRDLTCAVIT EFARIQLGAK GIQIKVPGEK DTQKVVVDLS 130 140 150 160 170 180 EQEEEKVELK ESENLASGDQ PRTAAVGEIC EEGLHVLEGL AASGLRSIRF ALEVPGLRSV 190 200 210 220 230 240 VANDASTRAV DLIRRNVQLN DVAHLVQPSQ ADARMLMYQH QRVSERFDVI DLDPYGSPAT 250 260 270 280 290 300 FLDAAVQAVS EGGLLCVTCT DMAVLAGNSG ETCYSKYGAM ALKSRACHEM ALRIVLHSLD 310 320 330 340 350 360 LRANCYQRFV VPLLSISADF YVRVFVRVFT GQAKVKASAS KQALVFQCVG CGAFHLQRLG 370 380 390 400 410 420 KASGVPSGRA KFSAACGPPV TPECEHCGQR HQLGGPMWAE PIHDLDFVGR VLEAVSANPG 430 440 450 460 470 480 RFHTSERIRG VLSVITEELP DVPLYYTLDQ LSSTIHCNTP SLLQLRSALL HADFRVSLSH 490 500 510 520 530 540 ACKNAVKTDA PASALWDIMR CWEKECPVKR ERLSETSPAF RILSVEPRLQ ANFTIREDAN 550 560 570 580 590 600 PSSRQRGLKR FQANPEANWG PRPRARPGGK AADEAMEERR RLLQNKRKEP PEDVAQRAAR 610 620 630 640 650 LKTFPCKRFK EGTCQRGDQC CYSHSPPTPR VSADAAPDCP ETSNQTPPGP GAAAGPGID
	Isoform 2. Checksum: 2CE1AF04EED60391	FASTA	630	69,306
	10 20 30 40 50 60 MQGSSLWLSL TFRSARVLSR ARFFEWQSPG LPNTAAMENG TGPYGEERPR EVQETTVTEG 70 80 90 100 110 120 AAKIAFPSAN EVFYNPVQEF NRDLTCAVIT EFARIQLGAK GIQIKVPGEK DTQKVVVDLS 130 140 150 160 170 180 EQEEEKVELK ESENLASGDQ PRTAAVGEIC EEGLHVLEGL AASGLRSIRF ALEVPGLRSV 190 200 210 220 230 240 VANDASTRAV DLIRRNVQLN DVAHLVQPSQ ADARMLMYQH QRVSERFDVI DLDPYGSPAT 250 260 270 280 290 300 FLDAAVQAVS EGGLLCVTCT DMAVLAGNSG ETCYSKYGAM ALKSRACHEM ALRIVLHSLD 310 320 330 340 350 360 LRANCYQRFV VPLLSISADF YVRVFVRVFT GQAKVKASAR AKFSAACGPP VTPECEHCGQ 370 380 390 400 410 420 RHQLGGPMWA EPIHDLDFVG RVLEAVSANP GRFHTSERIR GVLSVITEEL PDVPLYYTLD 430 440 450 460 470 480 QLSSTIHCNT PSLLQLRSAL LHADFRVSLS HACKNAVKTD APASALWDIM RCWEKECPVK 490 500 510 520 530 540 RERLSETSPA FRILSVEPRL QANFTIREDA NPSSRQRGLK RFQANPEANW GPRPRARPGG 550 560 570 580 590 600 KAADEAMEER RRLLQNKRKE PPEDVAQRAA RLKTFPCKRF KEGTCQRGDQ CCYSHSPPTP 610 620 630 RVSADAAPDC PETSNQTPPG PGAAAGPGID

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human tRNA(m22G26)dimethyltransferase: functional expression and characterization of a cloned hTRM1 gene." Liu J., Straby K.B. Nucleic Acids Res. 28:3445-3451(2000) [PubMed: 10982862] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Placenta.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Colon mucosa.
[3]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain, Cervix and Colon.
[5]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-646, MASS SPECTROMETRY. Tissue: Epithelium.
[6]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-628, MASS SPECTROMETRY.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-625; THR-628 AND THR-646, MASS SPECTROMETRY.
[8]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF196479 mRNA. Translation: AAG28495.1. AK000251 mRNA. Translation: BAA91031.1. AC005546 Genomic DNA. Translation: AAC33150.1. BC002492 mRNA. Translation: AAH02492.1. BC018302 mRNA. Translation: AAH18302.1. BC040126 mRNA. Translation: AAH40126.1.
IPI	IPI00020508. IPI00178861.
RefSeq	NP_001129507.1. NP_001136026.1. NP_060192.1.
UniGene	Hs.515169
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NXH9. 1 interaction.
PTM databases
PhosphoSite	Q9NXH9.
Proteomic databases
PRIDE	Q9NXH9.
Genome annotation databases
Ensembl	ENSG00000104907. Homo sapiens. [Contig view]
GeneID	55621.
KEGG	hsa:55621.
UCSC	uc002mwj.1. human. uc002mwk.1. human.
Organism-specific databases
GeneCards	GC19M013077.
H-InvDB	HIX0014819.
HGNC	HGNC:25980. TRMT1.
MIM	611669. gene.
PharmGKB	PA134867808.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9NXH9.
HOVERGEN	Q9NXH9.
OMA	Q9NXH9. AAMENGT.
Enzyme and pathway databases
BRENDA	2.1.1.32. 247.
Gene expression databases
ArrayExpress	Q9NXH9.
Bgee	Q9NXH9.
CleanEx	HS_TRMT1.
GermOnline	ENSG00000104907. Homo sapiens.
Family and domain databases
InterPro	IPR002905. TRM_MeTrfase. IPR000571. Znf_CCCH. [Graphical view]
PANTHER	PTHR10631. TRM_mtfrase. 1 hit.
Pfam	PF02005. TRM. 1 hit. PF00642. zf-CCCH. 1 hit. [Graphical view]
SMART	SM00356. ZnF_C3H1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00308. TRM1. 1 hit.
PROSITE	PS50103. ZF_C3H1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	60228.
SOURCE	Search...

Entry information

Entry name

TRM1_HUMAN

Accession

Primary (citable) accession number: Q9NXH9
Secondary accession number(s): O76103, Q548Y5, Q8WVA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	October 1, 2000
Last modified:	July 7, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)