Q9NXH3 (PP14D_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 1 regulatory subunit 14D

Alternative name(s):
Gastrointestinal and brain-specific PP1-inhibitory protein 1
Short name=GBPI-1

Gene names

Name:	PPP1R14D
Synonyms:	GBPI

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	145 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibitor of PPP1CA. Has inhibitory activity only when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction. Ref.1
Subcellular location	Cytoplasm Probable.
Tissue specificity	Detected in colon, intestine, kidney and brain cortex. Ref.1
Post-translational modification	Phosphorylated on several residues. Ref.1
Sequence similarities	Belongs to the PP1 inhibitor family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Protein phosphatase inhibitor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein phosphatase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 145	145	Protein phosphatase 1 regulatory subunit 14D		PRO_0000071497
Regions
	Region	21 – 25	5	Interaction with protein phosphatase 1
Amino acid modifications
	Modified residue	58	1	Phosphothreonine
Experimental info
	Mutagenesis	21 – 22	2	KK → EE: Reduces inhibitory activity by 57%.
	Mutagenesis	25	1	W → A: Reduces inhibitory activity by 13%. Ref.1
	Mutagenesis	58	1	T → E: Reduces inhibitory activity by 16%. Reduces phosphorylation. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9NXH3 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 012899C72C5760A4	FASTA	145	16,508
	10 20 30 40 50 60 MLSSSPASCT SPSPDGENPC KKVHWASGRR RTSSTDSESK SHPDSSKIPR SRRPSRLTVK 70 80 90 100 110 120 YDRGQLQRWL EMEQWVDAQV QELFQDQATP SEPEIDLEAL MDLSTEEQKT QLEAILGNCP 130 140 RPTEAFISEL LSQLKKLRRL SRPQK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"GBPI, a novel gastrointestinal- and brain-specific PP1-inhibitory protein, is activated by PKC and inactivated by PKA." Liu Q.-R., Zhang P.-W., Lin Z., Li Q.-F., Woods A.S., Troncoso J., Uhl G.R. Biochem. J. 377:171-181(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, PHOSPHORYLATION, MUTAGENESIS OF 21-LYS-LYS-22; TRP-25 AND THR-58, TISSUE SPECIFICITY.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon mucosa.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY050671 mRNA. Translation: AAL25829.1. AK000258 mRNA. Translation: BAA91037.1. BC096716 mRNA. Translation: AAH96716.1. BC098124 mRNA. Translation: AAH98124.1.
IPI	IPI00015106.
RefSeq	NP_060196.1. NM_017726.7.
UniGene	Hs.192927.
3D structure databases
ProteinModelPortal	Q9NXH3.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000398342.
PTM databases
PhosphoSite	Q9NXH3.
Polymorphism databases
DMDM	55583999.
Proteomic databases
PaxDb	Q9NXH3.
PRIDE	Q9NXH3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000299174; ENSP00000299174; ENSG00000166143.
GeneID	54866.
KEGG	hsa:54866.
UCSC	uc001zmy.3. human.
Organism-specific databases
CTD	54866.
GeneCards	GC15M041107.
HGNC	HGNC:14953. PPP1R14D.
HPA	HPA041846.
MIM	613256. gene.
neXtProt	NX_Q9NXH3.
PharmGKB	PA33631.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG81055.
HOGENOM	HOG000231993.
HOVERGEN	HBG054419.
InParanoid	Q9NXH3.
OrthoDB	EOG4MKNHW.
PhylomeDB	Q9NXH3.
Gene expression databases
ArrayExpress	Q9NXH3.
Bgee	Q9NXH3.
CleanEx	HS_PPP1R14D.
Genevestigator	Q9NXH3.
GermOnline	ENSG00000166143. Homo sapiens.
Family and domain databases
Gene3D	1.10.150.220. 1 hit.
InterPro	IPR008025. PP1_inhibitor. [Graphical view]
PANTHER	PTHR16188. PTHR16188. 1 hit.
Pfam	PF05361. PP1_inhibitor. 1 hit. [Graphical view]
SUPFAM	SSF81790. PP1_inhibitor. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	54866.
NextBio	57766.
SOURCE	Search...

Entry information

Entry name

PP14D_HUMAN

Accession

Primary (citable) accession number: Q9NXH3
Secondary accession number(s): Q4V773

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 9, 2004
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.