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Protein

Protein phosphatase 1 regulatory subunit 14D

Gene

PPP1R14D

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor of PPP1CA. Has inhibitory activity only when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protein phosphatase inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory subunit 14D
Alternative name(s):
Gastrointestinal and brain-specific PP1-inhibitory protein 1
Short name:
GBPI-1
Gene namesi
Name:PPP1R14D
Synonyms:GBPI
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:14953. PPP1R14D.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi21 – 222KK → EE: Reduces inhibitory activity by 57%. 1 Publication
Mutagenesisi25 – 251W → A: Reduces inhibitory activity by 13%. 1 Publication
Mutagenesisi58 – 581T → E: Reduces inhibitory activity by 16%. Reduces phosphorylation. 1 Publication

Organism-specific databases

PharmGKBiPA33631.

Polymorphism and mutation databases

BioMutaiPPP1R14D.
DMDMi55583999.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 145145Protein phosphatase 1 regulatory subunit 14DPRO_0000071497Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei58 – 581Phosphothreonine1 Publication

Post-translational modificationi

Phosphorylated on several residues.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9NXH3.
PRIDEiQ9NXH3.

PTM databases

iPTMnetiQ9NXH3.
PhosphoSiteiQ9NXH3.

Expressioni

Tissue specificityi

Detected in colon, intestine, kidney and brain cortex.1 Publication

Gene expression databases

BgeeiQ9NXH3.
CleanExiHS_PPP1R14D.
ExpressionAtlasiQ9NXH3. baseline and differential.
GenevisibleiQ9NXH3. HS.

Organism-specific databases

HPAiHPA041846.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000398342.

Structurei

3D structure databases

ProteinModelPortaliQ9NXH3.
SMRiQ9NXH3. Positions 56-139.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 255Interaction with protein phosphatase 1

Sequence similaritiesi

Belongs to the PP1 inhibitor family.Curated

Phylogenomic databases

eggNOGiENOG410J01A. Eukaryota.
ENOG41115QK. LUCA.
GeneTreeiENSGT00390000003608.
HOGENOMiHOG000231993.
HOVERGENiHBG054419.
InParanoidiQ9NXH3.
KOiK17557.
OrthoDBiEOG7RNK21.
PhylomeDBiQ9NXH3.

Family and domain databases

Gene3Di1.10.150.220. 1 hit.
InterProiIPR008025. PP1_inhibitor.
[Graphical view]
PANTHERiPTHR16188. PTHR16188. 1 hit.
PfamiPF05361. PP1_inhibitor. 1 hit.
[Graphical view]
SUPFAMiSSF81790. SSF81790. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9NXH3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSSSPASCT SPSPDGENPC KKVHWASGRR RTSSTDSESK SHPDSSKIPR
60 70 80 90 100
SRRPSRLTVK YDRGQLQRWL EMEQWVDAQV QELFQDQATP SEPEIDLEAL
110 120 130 140
MDLSTEEQKT QLEAILGNCP RPTEAFISEL LSQLKKLRRL SRPQK
Length:145
Mass (Da):16,508
Last modified:October 1, 2000 - v1
Checksum:i012899C72C5760A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY050671 mRNA. Translation: AAL25829.1.
AK000258 mRNA. Translation: BAA91037.1.
BC096716 mRNA. Translation: AAH96716.1.
BC098124 mRNA. Translation: AAH98124.1.
CCDSiCCDS10066.1.
RefSeqiNP_060196.1. NM_017726.7.
UniGeneiHs.192927.

Genome annotation databases

EnsembliENST00000299174; ENSP00000299174; ENSG00000166143.
GeneIDi54866.
KEGGihsa:54866.
UCSCiuc001zmy.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY050671 mRNA. Translation: AAL25829.1.
AK000258 mRNA. Translation: BAA91037.1.
BC096716 mRNA. Translation: AAH96716.1.
BC098124 mRNA. Translation: AAH98124.1.
CCDSiCCDS10066.1.
RefSeqiNP_060196.1. NM_017726.7.
UniGeneiHs.192927.

3D structure databases

ProteinModelPortaliQ9NXH3.
SMRiQ9NXH3. Positions 56-139.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000398342.

PTM databases

iPTMnetiQ9NXH3.
PhosphoSiteiQ9NXH3.

Polymorphism and mutation databases

BioMutaiPPP1R14D.
DMDMi55583999.

Proteomic databases

PaxDbiQ9NXH3.
PRIDEiQ9NXH3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000299174; ENSP00000299174; ENSG00000166143.
GeneIDi54866.
KEGGihsa:54866.
UCSCiuc001zmy.3. human.

Organism-specific databases

CTDi54866.
GeneCardsiPPP1R14D.
HGNCiHGNC:14953. PPP1R14D.
HPAiHPA041846.
MIMi613256. gene.
neXtProtiNX_Q9NXH3.
PharmGKBiPA33631.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410J01A. Eukaryota.
ENOG41115QK. LUCA.
GeneTreeiENSGT00390000003608.
HOGENOMiHOG000231993.
HOVERGENiHBG054419.
InParanoidiQ9NXH3.
KOiK17557.
OrthoDBiEOG7RNK21.
PhylomeDBiQ9NXH3.

Miscellaneous databases

GenomeRNAii54866.
PROiQ9NXH3.
SOURCEiSearch...

Gene expression databases

BgeeiQ9NXH3.
CleanExiHS_PPP1R14D.
ExpressionAtlasiQ9NXH3. baseline and differential.
GenevisibleiQ9NXH3. HS.

Family and domain databases

Gene3Di1.10.150.220. 1 hit.
InterProiIPR008025. PP1_inhibitor.
[Graphical view]
PANTHERiPTHR16188. PTHR16188. 1 hit.
PfamiPF05361. PP1_inhibitor. 1 hit.
[Graphical view]
SUPFAMiSSF81790. SSF81790. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "GBPI, a novel gastrointestinal- and brain-specific PP1-inhibitory protein, is activated by PKC and inactivated by PKA."
    Liu Q.-R., Zhang P.-W., Lin Z., Li Q.-F., Woods A.S., Troncoso J., Uhl G.R.
    Biochem. J. 377:171-181(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, PHOSPHORYLATION, MUTAGENESIS OF 21-LYS-LYS-22; TRP-25 AND THR-58, TISSUE SPECIFICITY.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Colon mucosa.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiPP14D_HUMAN
AccessioniPrimary (citable) accession number: Q9NXH3
Secondary accession number(s): Q4V773
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 9, 2004
Last sequence update: October 1, 2000
Last modified: June 8, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.