Q9NXF8 (ZDHC7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Palmitoyltransferase ZDHHC7
EC=2.3.1.-

Alternative name(s):
Zinc finger DHHC domain-containing protein 7
Short name=DHHC-7
Zinc finger protein 370

Gene names

Name:	ZDHHC7
Synonyms:	ZNF370

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Palmitoyltransferase with broad specificity. Palmitoylates SNAP25 and DLG4/PSD95. May palmitoylate GABA receptors on their gamma subunit (GABRG1, GABRG2 and GABRG3) and thereby regulate their synaptic clustering and/or cell surface stability By similarity. Palmitoylates sex steroid hormone receptors, including ESR1, PGR and AR, thereby regulating their targeting to the plasma membrane. This affects rapid intracellular signaling by sex hormones via ERK and AKT kinases and the generation of cAMP, but does not affect that mediated by their nuclear receptors. Ref.4
Catalytic activity	Palmitoyl-CoA + protein-cysteine = S-palmitoyl protein + CoA.
Subcellular location	Membrane; Multi-pass membrane protein Potential. Golgi apparatus Ref.4.
Domain	The DHHC domain is required for palmitoyltransferase activity By similarity.
Sequence similarities	Belongs to the DHHC palmitoyltransferase family. Contains 1 DHHC-type zinc finger.
Sequence caution	The sequence AAH17702.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAA91814.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptidyl-L-cysteine S-palmitoylation Inferred from mutant phenotype Ref.4. Source: UniProtKB
Cellular_component	Golgi apparatus Inferred from direct assay Ref.4. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	palmitoyltransferase activity Inferred from electronic annotation. Source: Compara zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NXF8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NXF8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     105-105: P → PEKSSDCRPSACTVKTGLDPTLVGICGEGTESVQSLLL

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Palmitoyltransferase ZDHHC7		PRO_0000212874
Regions
	Transmembrane	51 – 71	21	Helical; Potential
	Transmembrane	76 – 96	21	Helical; Potential
	Transmembrane	175 – 195	21	Helical; Potential
	Transmembrane	218 – 238	21	Helical; Potential
	Zinc finger	130 – 180	51	DHHC-type
Natural variations
	Alternative sequence	105	1	P → PEKSSDCRPSACTVKTGLDP TLVGICGEGTESVQSLLL in isoform 2.		VSP_006942
	Natural variant	44	1	D → N in a colorectal cancer sample; somatic mutation. Ref.5		VAR_036261
	Natural variant	201	1	V → I. Corresponds to variant rs13334011 [ dbSNP | Ensembl ].		VAR_028360
Experimental info
	Sequence conflict	22	1	N → D in BAA91055. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 17, 2006. Version 2. Checksum: 0E4F2E69A62C90C3	FASTA	308	35,140
	10 20 30 40 50 60 MQPSGHRLRD VEHHPLLAEN DNYDSSSSSS SEADVADRVW FIRDGCGMIC AVMTWLLVAY 70 80 90 100 110 120 ADFVVTFVML LPSKDFWYSV VNGVIFNCLA VLALSSHLRT MLTDPGAVPK GNATKEYMES 130 140 150 160 170 180 LQLKPGEVIY KCPKCCCIKP ERAHHCSICK RCIRKMDHHC PWVNNCVGEK NQRFFVLFTM 190 200 210 220 230 240 YIALSSVHAL ILCGFQFISC VRGQWTECSD FSPPITVILL IFLCLEGLLF FTFTAVMFGT 250 260 270 280 290 300 QIHSICNDET EIERLKSEKP TWERRLRWEG MKSVFGGPPS LLWMNPFVGF RFRRLPTRPR KGGPEFSV
	Isoform 2 [UniParc]. Checksum: 70448128ABCF6626	FASTA	345	38,917
	10 20 30 40 50 60 MQPSGHRLRD VEHHPLLAEN DNYDSSSSSS SEADVADRVW FIRDGCGMIC AVMTWLLVAY 70 80 90 100 110 120 ADFVVTFVML LPSKDFWYSV VNGVIFNCLA VLALSSHLRT MLTDPEKSSD CRPSACTVKT 130 140 150 160 170 180 GLDPTLVGIC GEGTESVQSL LLGAVPKGNA TKEYMESLQL KPGEVIYKCP KCCCIKPERA 190 200 210 220 230 240 HHCSICKRCI RKMDHHCPWV NNCVGEKNQR FFVLFTMYIA LSSVHALILC GFQFISCVRG 250 260 270 280 290 300 QWTECSDFSP PITVILLIFL CLEGLLFFTF TAVMFGTQIH SICNDETEIE RLKSEKPTWE 310 320 330 340 RRLRWEGMKS VFGGPPSLLW MNPFVGFRFR RLPTRPRKGG PEFSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Hepatoma and Teratocarcinoma.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 97-308 (ISOFORM 1). Tissue: Duodenal mucosa and Skin.
[4]	"DHHC-7 and -21 are palmitoylacyltransferases for sex steroid receptors." Pedram A., Razandi M., Deschenes R.J., Levin E.R. Mol. Biol. Cell 23:188-199(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] ASN-44.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK000286 mRNA. Translation: BAA91055.1. AK001654 mRNA. Translation: BAA91814.1. Different initiation. CH471114 Genomic DNA. Translation: EAW95464.1. CH471114 Genomic DNA. Translation: EAW95465.1. BC017702 mRNA. Translation: AAH17702.1. Different initiation. BC018772 mRNA. Translation: AAH18772.1.
IPI	IPI00015608. IPI00221179.
RefSeq	NP_001139020.1. NM_001145548.1. NP_060210.2. NM_017740.2.
UniGene	Hs.461610. Hs.723447.
3D structure databases
ProteinModelPortal	Q9NXF8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NXF8. 2 interactions.
MINT	MINT-1036602.
STRING	9606.ENSP00000341681.
PTM databases
PhosphoSite	Q9NXF8.
Polymorphism databases
DMDM	116242853.
Proteomic databases
PaxDb	Q9NXF8.
PRIDE	Q9NXF8.
Protocols and materials databases
DNASU	55625.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000313732; ENSP00000315604; ENSG00000153786. ENST00000344861; ENSP00000341681; ENSG00000153786. ENST00000564466; ENSP00000456782; ENSG00000153786.
GeneID	55625.
KEGG	hsa:55625.
UCSC	uc002fiq.2. human. uc010voi.1. human.
Organism-specific databases
CTD	55625.
GeneCards	GC16M085007.
HGNC	HGNC:18459. ZDHHC7.
MIM	614604. gene.
neXtProt	NX_Q9NXF8.
PharmGKB	PA38335.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5273.
HOGENOM	HOG000234755.
HOVERGEN	HBG056583.
OMA	FRFRRLQ.
OrthoDB	EOG4FBHT8.
Gene expression databases
ArrayExpress	Q9NXF8.
Bgee	Q9NXF8.
CleanEx	HS_ZDHHC7.
Genevestigator	Q9NXF8.
GermOnline	ENSG00000153786. Homo sapiens.
Family and domain databases
InterPro	IPR001594. Znf_DHHC_palmitoyltrfase. [Graphical view]
Pfam	PF01529. zf-DHHC. 1 hit. [Graphical view]
PROSITE	PS50216. ZF_DHHC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ZDHHC7. human.
GenomeRNAi	55625.
NextBio	60244.
SOURCE	Search...

Entry information

Entry name

ZDHC7_HUMAN

Accession

Primary (citable) accession number: Q9NXF8
Secondary accession number(s): D3DUM1, Q8WV42, Q9NVD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2003
Last sequence update:	October 17, 2006
Last modified:	April 3, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.