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Protein

Pre-mRNA-splicing factor CWC25 homolog

Gene

CWC25

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor CWC25 homolog
Alternative name(s):
Coiled-coil domain-containing protein 49
Spliceosome-associated protein homolog CWC25
Gene namesi
Name:CWC25
Synonyms:CCDC49
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:25989. CWC25.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA165431769.

Polymorphism and mutation databases

DMDMi74734693.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 425425Pre-mRNA-splicing factor CWC25 homologPRO_0000288865Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei218 – 2181Phosphoserine2 Publications
Modified residuei222 – 2221Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9NXE8.
PaxDbiQ9NXE8.
PRIDEiQ9NXE8.

PTM databases

PhosphoSiteiQ9NXE8.

Expressioni

Gene expression databases

BgeeiQ9NXE8.
CleanExiHS_CCDC49.
ExpressionAtlasiQ9NXE8. baseline and differential.
GenevisibleiQ9NXE8. HS.

Organism-specific databases

HPAiCAB014874.
HPA044334.

Interactioni

Protein-protein interaction databases

BioGridi120231. 4 interactions.
IntActiQ9NXE8. 3 interactions.
MINTiMINT-1440092.
STRINGi9606.ENSP00000225428.

Structurei

3D structure databases

ProteinModelPortaliQ9NXE8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili20 – 7051Sequence AnalysisAdd
BLAST
Coiled coili333 – 37745Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi144 – 19047Lys-richAdd
BLAST

Sequence similaritiesi

Belongs to the CWC25 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG296370.
GeneTreeiENSGT00440000039055.
HOGENOMiHOG000006950.
HOVERGENiHBG061012.
InParanoidiQ9NXE8.
OMAiLEDPLML.
OrthoDBiEOG7TTQ83.
PhylomeDBiQ9NXE8.
TreeFamiTF320801.

Family and domain databases

InterProiIPR019339. CIR_N_dom.
IPR022209. CWC25.
[Graphical view]
PfamiPF10197. Cir_N. 1 hit.
PF12542. CWC25. 1 hit.
[Graphical view]
SMARTiSM01083. Cir_N. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NXE8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGGGDLNLKK SWHPQTLRNV EKVWKAEQKH EAERKKIEEL QRELREERAR
60 70 80 90 100
EEMQRYAEDV GAVKKKEEKL DWMYQGPGGM VNRDEYLLGR PIDKYVFEKM
110 120 130 140 150
EEKEAGCSSE TGLLPGSIFA PSGANSLLDM ASKIREDPLF IIRKKEEEKK
160 170 180 190 200
REVLNNPVKM KKIKELLQMS LEKKEKKKKK EKKKKHKKHK HRSSSSDRSS
210 220 230 240 250
SEDEHSAGRS QKKMANSSPV LSKVPGYGLQ VRNSDRNQGL QGPLTAEQKR
260 270 280 290 300
GHGMKNHSRS RSSSHSPPRH ASKKSTREAG SRDRRSRSLG RRSRSPRPSK
310 320 330 340 350
LHNSKVNRRE TGQTRSPSPK KEVYQRRHAP GYTRKLSAEE LERKRQEMME
360 370 380 390 400
NAKWREEERL NILKRHAKDE EREQRLEKLD SRDGKFIHRM KLESASTSSL
410 420
EDRVKRNIYS LQRTSVALEK NFMKR
Length:425
Mass (Da):49,647
Last modified:October 1, 2000 - v1
Checksum:iF8288E143A3FF764
GO
Isoform 2 (identifier: Q9NXE8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-347: Missing.

Show »
Length:78
Mass (Da):9,522
Checksum:iAF7DB596F46C98C4
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 347347Missing in isoform 2. 1 PublicationVSP_025797Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000298 mRNA. Translation: BAA91065.1.
CR749362 mRNA. Translation: CAH18215.1.
BC003085 mRNA. Translation: AAH03085.1. Different termination.
BC008833 mRNA. Translation: AAH08833.1.
CCDSiCCDS45663.1. [Q9NXE8-1]
RefSeqiNP_060218.1. NM_017748.4. [Q9NXE8-1]
UniGeneiHs.406223.

Genome annotation databases

EnsembliENST00000614790; ENSP00000478070; ENSG00000273559. [Q9NXE8-1]
GeneIDi54883.
KEGGihsa:54883.
UCSCiuc002hqu.4. human. [Q9NXE8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000298 mRNA. Translation: BAA91065.1.
CR749362 mRNA. Translation: CAH18215.1.
BC003085 mRNA. Translation: AAH03085.1. Different termination.
BC008833 mRNA. Translation: AAH08833.1.
CCDSiCCDS45663.1. [Q9NXE8-1]
RefSeqiNP_060218.1. NM_017748.4. [Q9NXE8-1]
UniGeneiHs.406223.

3D structure databases

ProteinModelPortaliQ9NXE8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120231. 4 interactions.
IntActiQ9NXE8. 3 interactions.
MINTiMINT-1440092.
STRINGi9606.ENSP00000225428.

PTM databases

PhosphoSiteiQ9NXE8.

Polymorphism and mutation databases

DMDMi74734693.

Proteomic databases

MaxQBiQ9NXE8.
PaxDbiQ9NXE8.
PRIDEiQ9NXE8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000614790; ENSP00000478070; ENSG00000273559. [Q9NXE8-1]
GeneIDi54883.
KEGGihsa:54883.
UCSCiuc002hqu.4. human. [Q9NXE8-1]

Organism-specific databases

CTDi54883.
GeneCardsiGC17M036957.
HGNCiHGNC:25989. CWC25.
HPAiCAB014874.
HPA044334.
neXtProtiNX_Q9NXE8.
PharmGKBiPA165431769.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG296370.
GeneTreeiENSGT00440000039055.
HOGENOMiHOG000006950.
HOVERGENiHBG061012.
InParanoidiQ9NXE8.
OMAiLEDPLML.
OrthoDBiEOG7TTQ83.
PhylomeDBiQ9NXE8.
TreeFamiTF320801.

Miscellaneous databases

GenomeRNAii54883.
NextBioi57855.
PROiQ9NXE8.

Gene expression databases

BgeeiQ9NXE8.
CleanExiHS_CCDC49.
ExpressionAtlasiQ9NXE8. baseline and differential.
GenevisibleiQ9NXE8. HS.

Family and domain databases

InterProiIPR019339. CIR_N_dom.
IPR022209. CWC25.
[Graphical view]
PfamiPF10197. Cir_N. 1 hit.
PF12542. CWC25. 1 hit.
[Graphical view]
SMARTiSM01083. Cir_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Hepatoma.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Eye and Uterus.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-218 AND SER-222, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-218, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCWC25_HUMAN
AccessioniPrimary (citable) accession number: Q9NXE8
Secondary accession number(s): A0JLM3, Q68DK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 1, 2000
Last modified: June 24, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.