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Q9NXE8 (CWC25_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pre-mRNA-splicing factor CWC25 homolog
Alternative name(s):
Coiled-coil domain-containing protein 49
Spliceosome-associated protein homolog CWC25
Gene names
Name:CWC25
Synonyms:CCDC49
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length425 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the CWC25 family.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9NXE8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9NXE8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-347: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 425425Pre-mRNA-splicing factor CWC25 homolog
PRO_0000288865

Regions

Coiled coil20 – 7051 Potential
Coiled coil333 – 37745 Potential
Compositional bias144 – 19047Lys-rich

Amino acid modifications

Modified residue2181Phosphoserine Ref.5 Ref.6
Modified residue2221Phosphoserine Ref.5

Natural variations

Alternative sequence1 – 347347Missing in isoform 2.
VSP_025797

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: F8288E143A3FF764

FASTA42549,647
        10         20         30         40         50         60 
MGGGDLNLKK SWHPQTLRNV EKVWKAEQKH EAERKKIEEL QRELREERAR EEMQRYAEDV 

        70         80         90        100        110        120 
GAVKKKEEKL DWMYQGPGGM VNRDEYLLGR PIDKYVFEKM EEKEAGCSSE TGLLPGSIFA 

       130        140        150        160        170        180 
PSGANSLLDM ASKIREDPLF IIRKKEEEKK REVLNNPVKM KKIKELLQMS LEKKEKKKKK 

       190        200        210        220        230        240 
EKKKKHKKHK HRSSSSDRSS SEDEHSAGRS QKKMANSSPV LSKVPGYGLQ VRNSDRNQGL 

       250        260        270        280        290        300 
QGPLTAEQKR GHGMKNHSRS RSSSHSPPRH ASKKSTREAG SRDRRSRSLG RRSRSPRPSK 

       310        320        330        340        350        360 
LHNSKVNRRE TGQTRSPSPK KEVYQRRHAP GYTRKLSAEE LERKRQEMME NAKWREEERL 

       370        380        390        400        410        420 
NILKRHAKDE EREQRLEKLD SRDGKFIHRM KLESASTSSL EDRVKRNIYS LQRTSVALEK 


NFMKR 

« Hide

Isoform 2 [UniParc].

Checksum: AF7DB596F46C98C4
Show »

FASTA789,522

References

[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Hepatoma.
[2]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Liver.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Eye and Uterus.
[4]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[5]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-218 AND SER-222, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[6]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-218, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK000298 mRNA. Translation: BAA91065.1.
CR749362 mRNA. Translation: CAH18215.1.
BC003085 mRNA. Translation: AAH03085.1. Different termination.
BC008833 mRNA. Translation: AAH08833.1.
CCDSCCDS45663.1. [Q9NXE8-1]
RefSeqNP_060218.1. NM_017748.4. [Q9NXE8-1]
UniGeneHs.406223.

3D structure databases

ProteinModelPortalQ9NXE8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120231. 4 interactions.
IntActQ9NXE8. 3 interactions.
MINTMINT-1440092.

PTM databases

PhosphoSiteQ9NXE8.

Polymorphism databases

DMDM74734693.

Proteomic databases

MaxQBQ9NXE8.
PaxDbQ9NXE8.
PRIDEQ9NXE8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000225428; ENSP00000225428; ENSG00000108296. [Q9NXE8-1]
GeneID54883.
KEGGhsa:54883.
UCSCuc002hqu.4. human. [Q9NXE8-1]

Organism-specific databases

CTD54883.
GeneCardsGC17M036957.
HGNCHGNC:25989. CWC25.
HPACAB014874.
HPA044334.
neXtProtNX_Q9NXE8.
PharmGKBPA165431769.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG296370.
HOGENOMHOG000006950.
HOVERGENHBG061012.
InParanoidQ9NXE8.
OMADRNQGLQ.
OrthoDBEOG7TTQ83.
PhylomeDBQ9NXE8.
TreeFamTF320801.

Gene expression databases

ArrayExpressQ9NXE8.
BgeeQ9NXE8.
CleanExHS_CCDC49.
GenevestigatorQ9NXE8.

Family and domain databases

InterProIPR019339. CIR_N_dom.
IPR022209. CWC25.
[Graphical view]
PfamPF10197. Cir_N. 1 hit.
PF12542. CWC25. 1 hit.
[Graphical view]
SMARTSM01083. Cir_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi54883.
NextBio57855.
PROQ9NXE8.

Entry information

Entry nameCWC25_HUMAN
AccessionPrimary (citable) accession number: Q9NXE8
Secondary accession number(s): A0JLM3, Q68DK5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM