Q9NXE4 (NSMA3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sphingomyelin phosphodiesterase 4
EC=3.1.4.12

Alternative name(s):
Neutral sphingomyelinase 3
Short name=nSMase-3
Short name=nSMase3
Neutral sphingomyelinase III

Gene names

Name:	SMPD4
Synonyms:	KIAA1418

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	827 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. Ref.7
Catalytic activity	Sphingomyelin + H2O = N-acylsphingosine + phosphocholine.
Cofactor	Magnesium. Ref.7
Enzyme regulation	Activated by phosphatidylserine and tumor necrosis factor (TNF). Inhibited by scyphostatin. Ref.7
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein. Golgi apparatus membrane; Single-pass membrane protein Ref.7.
Tissue specificity	Widely expressed, with highest levels in heart and skeletal muscle. Ref.7
Biophysicochemical properties	pH dependence: Optimum pH is 7.0. Ref.7
Sequence caution	The sequence BAA91368.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA91567.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA92656.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to tumor necrosis factor Inferred from direct assay Ref.7. Source: BHF-UCL ceramide biosynthetic process Inferred from direct assay Ref.7. Source: BHF-UCL glycerophospholipid catabolic process Inferred from direct assay Ref.7. Source: BHF-UCL glycosphingolipid metabolic process Traceable author statement. Source: Reactome sphingomyelin catabolic process Inferred from direct assay Ref.7. Source: HGNC
Cellular_component	Golgi apparatus Inferred from direct assay Ref.7. Source: HGNC Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Traceable author statement. Source: Reactome integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW trans-Golgi network Inferred from direct assay Ref.7. Source: HGNC
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW sphingomyelin phosphodiesterase D activity Inferred from direct assay Ref.7. Source: HGNC sphingomyelin phosphodiesterase activity Traceable author statement. Source: Reactome
Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NXE4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NXE4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     289-317: Missing.

Isoform 3 (identifier: Q9NXE4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-92: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q9NXE4-4)

The sequence of this isoform differs from the canonical sequence as follows:
     365-365: K → KRMGLNLPEVPSALPRRSQPSLMGALGSSVSRASLLGKQQVTLPIPC

Note: No experimental confirmation available.

Isoform 5 (identifier: Q9NXE4-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-429: Missing.
     430-484: WAPFVQENLL...QPNLAEMIQK → MSATYCVGDA...VGARFVSCLL

Note: No experimental confirmation available.

Isoform 6 (identifier: Q9NXE4-6)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MTTFGAVAEWRLPSLRRATLWIPQWFAKKAIFNSPLEAAM
     43-115: Missing.
     289-317: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 827	827	Sphingomyelin phosphodiesterase 4		PRO_0000273163
Regions
	Transmembrane	783 – 803	21	Helical; Potential
Amino acid modifications
	Modified residue	130	1	Phosphoserine Ref.8 Ref.9
	Modified residue	669	1	Phosphothreonine Ref.8
Natural variations
	Alternative sequence	1 – 429	429	Missing in isoform 5.		VSP_022479
	Alternative sequence	1 – 92	92	Missing in isoform 3.		VSP_022480
	Alternative sequence	1	1	M → MTTFGAVAEWRLPSLRRATL WIPQWFAKKAIFNSPLEAAM in isoform 6.		VSP_044494
	Alternative sequence	43 – 115	73	Missing in isoform 6.		VSP_044495
	Alternative sequence	289 – 317	29	Missing in isoform 2 and isoform 6.		VSP_022481
	Alternative sequence	365	1	K → KRMGLNLPEVPSALPRRSQP SLMGALGSSVSRASLLGKQQ VTLPIPC in isoform 4.		VSP_022482
	Alternative sequence	430 – 484	55	WAPFV…EMIQK → MSATYCVGDAARQGLRRTPL GHGSPDTSETPQNPKGSSRV LCLREVGARFVSCLL in isoform 5.		VSP_022483
Experimental info
	Sequence conflict	164	1	S → G in BAG61230. Ref.2
	Sequence conflict	540	1	C → R in BAA91567. Ref.2
	Sequence conflict	692	1	E → K in BAA91368. Ref.2
	Sequence conflict	693	1	L → Q in BAA91567. Ref.2
	Sequence conflict	703	1	S → G in BAC86751. Ref.2
	Sequence conflict	823	1	K → E in BAG61230. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: E9B68BF1D0895EC8	FASTA	827	93,352
	10 20 30 40 50 60 MAFPHLQQPS FLLASLKADS INKPFAQQCQ DLVKVIEDFP AKELHTIFPW LVESIFGSLD 70 80 90 100 110 120 GVLVGWNLRC LQGRVNPVEY SIVMEFLDPG GPMMKLVYKL QAEDYKFDFP VSYLPGPVKA 130 140 150 160 170 180 SIQECILPDS PLYHNKVQFT PTGGLGLNLA LNPFEYYIFF FALSLITQKP LPVSLHVRTS 190 200 210 220 230 240 DCAYFILVDR YLSWFLPTEG SVPPPLSSSP GGTSPSPPPR TPAIPFASYG LHHTSLLKRH 250 260 270 280 290 300 ISHQTSVNAD PASHEIWRSE TLLQVFVEMW LHHYSLEMYQ KMQSPHAKLE VLHYRLSVSS 310 320 330 340 350 360 ALYSPAQPSL QALHAYQESF TPTEEHVLVV RLLLKHLHAF ANSLKPEQAS PSAHSHATSP 370 380 390 400 410 420 LEEFKRAAVP RFVQQKLYLF LQHCFGHWPL DASFRAVLEM WLSYLQPWRY APDKQAPGSD 430 440 450 460 470 480 SQPRCVSEKW APFVQENLLM YTKLFVGFLN RALRTDLVSP KHALMVFRVA KVFAQPNLAE 490 500 510 520 530 540 MIQKGEQLFL EPELVIPHRQ HRLFTAPTFT GSFLSPWPPA VTDASFKVKS HVYSLEGQDC 550 560 570 580 590 600 KYTPMFGPEA RTLVLRLAQL ITQAKHTAKS ISDQCAESPA GHSFLSWLGF SSMDTNGSYT 610 620 630 640 650 660 ANDLDEMGQD SVRKTDEYLE KALEYLRQIF RLSEAQLRQF TLALGTTQDE NGKKQLPDCI 670 680 690 700 710 720 VGEDGLILTP LGRYQIINGL RRFEIEYQGD PELQPIRSYE IASLVRTLFR LSSAINHRFA 730 740 750 760 770 780 GQMAALCSRD DFLGSFCRYH LTEPGLASRH LLSPVGRRQV AGHTRGPRLS LRFLGSYRTL 790 800 810 820 VSLLLAFFVA SLFCVGPLPC TLLLTLGYVL YASAMTLLTE RGKLHQP
	Isoform 2 [UniParc]. Checksum: CB503C2FE7FF5260	FASTA	798	90,127
	10 20 30 40 50 60 MAFPHLQQPS FLLASLKADS INKPFAQQCQ DLVKVIEDFP AKELHTIFPW LVESIFGSLD 70 80 90 100 110 120 GVLVGWNLRC LQGRVNPVEY SIVMEFLDPG GPMMKLVYKL QAEDYKFDFP VSYLPGPVKA 130 140 150 160 170 180 SIQECILPDS PLYHNKVQFT PTGGLGLNLA LNPFEYYIFF FALSLITQKP LPVSLHVRTS 190 200 210 220 230 240 DCAYFILVDR YLSWFLPTEG SVPPPLSSSP GGTSPSPPPR TPAIPFASYG LHHTSLLKRH 250 260 270 280 290 300 ISHQTSVNAD PASHEIWRSE TLLQVFVEMW LHHYSLEMYQ KMQSPHAKES FTPTEEHVLV 310 320 330 340 350 360 VRLLLKHLHA FANSLKPEQA SPSAHSHATS PLEEFKRAAV PRFVQQKLYL FLQHCFGHWP 370 380 390 400 410 420 LDASFRAVLE MWLSYLQPWR YAPDKQAPGS DSQPRCVSEK WAPFVQENLL MYTKLFVGFL 430 440 450 460 470 480 NRALRTDLVS PKHALMVFRV AKVFAQPNLA EMIQKGEQLF LEPELVIPHR QHRLFTAPTF 490 500 510 520 530 540 TGSFLSPWPP AVTDASFKVK SHVYSLEGQD CKYTPMFGPE ARTLVLRLAQ LITQAKHTAK 550 560 570 580 590 600 SISDQCAESP AGHSFLSWLG FSSMDTNGSY TANDLDEMGQ DSVRKTDEYL EKALEYLRQI 610 620 630 640 650 660 FRLSEAQLRQ FTLALGTTQD ENGKKQLPDC IVGEDGLILT PLGRYQIING LRRFEIEYQG 670 680 690 700 710 720 DPELQPIRSY EIASLVRTLF RLSSAINHRF AGQMAALCSR DDFLGSFCRY HLTEPGLASR 730 740 750 760 770 780 HLLSPVGRRQ VAGHTRGPRL SLRFLGSYRT LVSLLLAFFV ASLFCVGPLP CTLLLTLGYV 790 LYASAMTLLT ERGKLHQP
	Isoform 3 [UniParc]. Checksum: D8AB07DB58C4FC79	FASTA	735	83,071
	10 20 30 40 50 60 MMKLVYKLQA EDYKFDFPVS YLPGPVKASI QECILPDSPL YHNKVQFTPT GGLGLNLALN 70 80 90 100 110 120 PFEYYIFFFA LSLITQKPLP VSLHVRTSDC AYFILVDRYL SWFLPTEGSV PPPLSSSPGG 130 140 150 160 170 180 TSPSPPPRTP AIPFASYGLH HTSLLKRHIS HQTSVNADPA SHEIWRSETL LQVFVEMWLH 190 200 210 220 230 240 HYSLEMYQKM QSPHAKLEVL HYRLSVSSAL YSPAQPSLQA LHAYQESFTP TEEHVLVVRL 250 260 270 280 290 300 LLKHLHAFAN SLKPEQASPS AHSHATSPLE EFKRAAVPRF VQQKLYLFLQ HCFGHWPLDA 310 320 330 340 350 360 SFRAVLEMWL SYLQPWRYAP DKQAPGSDSQ PRCVSEKWAP FVQENLLMYT KLFVGFLNRA 370 380 390 400 410 420 LRTDLVSPKH ALMVFRVAKV FAQPNLAEMI QKGEQLFLEP ELVIPHRQHR LFTAPTFTGS 430 440 450 460 470 480 FLSPWPPAVT DASFKVKSHV YSLEGQDCKY TPMFGPEART LVLRLAQLIT QAKHTAKSIS 490 500 510 520 530 540 DQCAESPAGH SFLSWLGFSS MDTNGSYTAN DLDEMGQDSV RKTDEYLEKA LEYLRQIFRL 550 560 570 580 590 600 SEAQLRQFTL ALGTTQDENG KKQLPDCIVG EDGLILTPLG RYQIINGLRR FEIEYQGDPE 610 620 630 640 650 660 LQPIRSYEIA SLVRTLFRLS SAINHRFAGQ MAALCSRDDF LGSFCRYHLT EPGLASRHLL 670 680 690 700 710 720 SPVGRRQVAG HTRGPRLSLR FLGSYRTLVS LLLAFFVASL FCVGPLPCTL LLTLGYVLYA 730 SAMTLLTERG KLHQP
	Isoform 4 [UniParc]. Checksum: 404647021BE6D555	FASTA	873	98,148
	10 20 30 40 50 60 MAFPHLQQPS FLLASLKADS INKPFAQQCQ DLVKVIEDFP AKELHTIFPW LVESIFGSLD 70 80 90 100 110 120 GVLVGWNLRC LQGRVNPVEY SIVMEFLDPG GPMMKLVYKL QAEDYKFDFP VSYLPGPVKA 130 140 150 160 170 180 SIQECILPDS PLYHNKVQFT PTGGLGLNLA LNPFEYYIFF FALSLITQKP LPVSLHVRTS 190 200 210 220 230 240 DCAYFILVDR YLSWFLPTEG SVPPPLSSSP GGTSPSPPPR TPAIPFASYG LHHTSLLKRH 250 260 270 280 290 300 ISHQTSVNAD PASHEIWRSE TLLQVFVEMW LHHYSLEMYQ KMQSPHAKLE VLHYRLSVSS 310 320 330 340 350 360 ALYSPAQPSL QALHAYQESF TPTEEHVLVV RLLLKHLHAF ANSLKPEQAS PSAHSHATSP 370 380 390 400 410 420 LEEFKRMGLN LPEVPSALPR RSQPSLMGAL GSSVSRASLL GKQQVTLPIP CRAAVPRFVQ 430 440 450 460 470 480 QKLYLFLQHC FGHWPLDASF RAVLEMWLSY LQPWRYAPDK QAPGSDSQPR CVSEKWAPFV 490 500 510 520 530 540 QENLLMYTKL FVGFLNRALR TDLVSPKHAL MVFRVAKVFA QPNLAEMIQK GEQLFLEPEL 550 560 570 580 590 600 VIPHRQHRLF TAPTFTGSFL SPWPPAVTDA SFKVKSHVYS LEGQDCKYTP MFGPEARTLV 610 620 630 640 650 660 LRLAQLITQA KHTAKSISDQ CAESPAGHSF LSWLGFSSMD TNGSYTANDL DEMGQDSVRK 670 680 690 700 710 720 TDEYLEKALE YLRQIFRLSE AQLRQFTLAL GTTQDENGKK QLPDCIVGED GLILTPLGRY 730 740 750 760 770 780 QIINGLRRFE IEYQGDPELQ PIRSYEIASL VRTLFRLSSA INHRFAGQMA ALCSRDDFLG 790 800 810 820 830 840 SFCRYHLTEP GLASRHLLSP VGRRQVAGHT RGPRLSLRFL GSYRTLVSLL LAFFVASLFC 850 860 870 VGPLPCTLLL TLGYVLYASA MTLLTERGKL HQP
	Isoform 5 [UniParc]. Checksum: 93961860F90846E4	FASTA	398	44,223
	10 20 30 40 50 60 MSATYCVGDA ARQGLRRTPL GHGSPDTSET PQNPKGSSRV LCLREVGARF VSCLLGEQLF 70 80 90 100 110 120 LEPELVIPHR QHRLFTAPTF TGSFLSPWPP AVTDASFKVK SHVYSLEGQD CKYTPMFGPE 130 140 150 160 170 180 ARTLVLRLAQ LITQAKHTAK SISDQCAESP AGHSFLSWLG FSSMDTNGSY TANDLDEMGQ 190 200 210 220 230 240 DSVRKTDEYL EKALEYLRQI FRLSEAQLRQ FTLALGTTQD ENGKKQLPDC IVGEDGLILT 250 260 270 280 290 300 PLGRYQIING LRRFEIEYQG DPELQPIRSY EIASLVRTLF RLSSAINHRF AGQMAALCSR 310 320 330 340 350 360 DDFLGSFCRY HLTEPGLASR HLLSPVGRRQ VAGHTRGPRL SLRFLGSYRT LVSLLLAFFV 370 380 390 ASLFCVGPLP CTLLLTLGYV LYASAMTLLT ERGKLHQP
	Isoform 6 [UniParc]. Checksum: B9110FD449F73E04	FASTA	764	86,193
	10 20 30 40 50 60 MTTFGAVAEW RLPSLRRATL WIPQWFAKKA IFNSPLEAAM AFPHLQQPSF LLASLKADSI 70 80 90 100 110 120 NKPFAQQCQD LVKVIEDFPA KGPVKASIQE CILPDSPLYH NKVQFTPTGG LGLNLALNPF 130 140 150 160 170 180 EYYIFFFALS LITQKPLPVS LHVRTSDCAY FILVDRYLSW FLPTEGSVPP PLSSSPGGTS 190 200 210 220 230 240 PSPPPRTPAI PFASYGLHHT SLLKRHISHQ TSVNADPASH EIWRSETLLQ VFVEMWLHHY 250 260 270 280 290 300 SLEMYQKMQS PHAKESFTPT EEHVLVVRLL LKHLHAFANS LKPEQASPSA HSHATSPLEE 310 320 330 340 350 360 FKRAAVPRFV QQKLYLFLQH CFGHWPLDAS FRAVLEMWLS YLQPWRYAPD KQAPGSDSQP 370 380 390 400 410 420 RCVSEKWAPF VQENLLMYTK LFVGFLNRAL RTDLVSPKHA LMVFRVAKVF AQPNLAEMIQ 430 440 450 460 470 480 KGEQLFLEPE LVIPHRQHRL FTAPTFTGSF LSPWPPAVTD ASFKVKSHVY SLEGQDCKYT 490 500 510 520 530 540 PMFGPEARTL VLRLAQLITQ AKHTAKSISD QCAESPAGHS FLSWLGFSSM DTNGSYTAND 550 560 570 580 590 600 LDEMGQDSVR KTDEYLEKAL EYLRQIFRLS EAQLRQFTLA LGTTQDENGK KQLPDCIVGE 610 620 630 640 650 660 DGLILTPLGR YQIINGLRRF EIEYQGDPEL QPIRSYEIAS LVRTLFRLSS AINHRFAGQM 670 680 690 700 710 720 AALCSRDDFL GSFCRYHLTE PGLASRHLLS PVGRRQVAGH TRGPRLSLRF LGSYRTLVSL 730 740 750 760 LLAFFVASLF CVGPLPCTLL LTLGYVLYAS AMTLLTERGK LHQP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 5 AND 6), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 134-827 (ISOFORM 4).
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Lung.
[5]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 397-827. Tissue: Testis.
[6]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 400-827.
[7]	"Novel tumor necrosis factor-responsive mammalian neutral sphingomyelinase-3 is a C-tail-anchored protein." Krut O., Wiegmann K., Kashkar H., Yazdanpanah B., Kroenke M. J. Biol. Chem. 281:13784-13793(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[8]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-130 AND THR-669, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-130, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB037839 mRNA. Translation: BAA92656.1. Different initiation. AK000304 mRNA. Translation: BAA91070.1. AK000763 mRNA. Translation: BAA91368.1. Different initiation. AK001227 mRNA. Translation: BAA91567.1. Different initiation. AK126920 mRNA. Translation: BAC86751.1. AK299185 mRNA. Translation: BAG61230.1. AC018804 Genomic DNA. Translation: AAY14883.1. BC064947 mRNA. Translation: AAH64947.1. AL136737 mRNA. Translation: CAB66671.2. CR533550 mRNA. Translation: CAG38581.1.
IPI	IPI00793691. IPI00827746. IPI00828125. IPI00828176. IPI00903175. IPI00910464.
RefSeq	NP_001164554.1. NM_001171083.2. NP_060221.2. NM_017751.4.
UniGene	Hs.516450.
3D structure databases
ProteinModelPortal	Q9NXE4.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NXE4. 1 interaction.
STRING	9606.ENSP00000376704.
PTM databases
PhosphoSite	Q9NXE4.
Polymorphism databases
DMDM	124015179.
Proteomic databases
PaxDb	Q9NXE4.
PRIDE	Q9NXE4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000431183; ENSP00000405187; ENSG00000136699.
GeneID	55627.
KEGG	hsa:55627.
UCSC	uc002tqo.2. human. uc002tqp.2. human. uc002tqr.2. human. uc002tqs.2. human.
Organism-specific databases
CTD	55627.
GeneCards	GC02M130908.
HGNC	HGNC:32949. SMPD4.
MIM	610457. gene.
neXtProt	NX_Q9NXE4.
PharmGKB	PA145148067.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG240539.
HOVERGEN	HBG082060.
InParanoid	Q9NXE4.
KO	K12353.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	Q9NXE4.
Bgee	Q9NXE4.
CleanEx	HS_SMPD4.
Genevestigator	Q9NXE4.
Family and domain databases
InterPro	IPR024129. Sphingomy_SMPD4. [Graphical view]
PANTHER	PTHR12988. PTHR12988. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	SMPD4. human.
DrugBank	DB00144. Phosphatidylserine.
GenomeRNAi	55627.
NextBio	60251.
SOURCE	Search...

Entry information

Entry name

NSMA3_HUMAN

Accession

Primary (citable) accession number: Q9NXE4
Secondary accession number(s): B4DRB8 , E7ESA2, Q6FI76, Q6P1P7, Q6ZT43, Q9H0M2, Q9NW20, Q9NWL2, Q9P2C9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.