Reviewed, UniProtKB/Swiss-Prot Q9NXA8 (SIRT5_HUMAN)

Names and origin

Protein names

Recommended name:
    NAD-dependent deacetylase sirtuin-5
    EC=3.5.1.-
Alternative name(s):
    SIR2-like protein 5

Gene names

Name:	SIRT5
Synonyms:	SIR2L5

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	NAD-dependent protein deacetylase that activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting. Deacetylates CPS1 and increases CPS1 activity in response to elevated NAD levels during fasting By similarity. Can deacetylate cytochrome c and a number of other proteins (in vitro). Ref.8
Catalytic activity	NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein. Ref.11
Cofactor	Binds 1 zinc ion per subunit.
Enzyme regulation	Inhibited by suramin. Ref.11
Subunit structure	Monomer. Homodimer. Forms homodimers upon suramin binding. Interacts with CPS1 By similarity. Ref.11
Subcellular location	Mitochondrion matrix. Mitochondrion intermembrane space Potential Ref.8 Ref.7.
Tissue specificity	Widely expressed. Ref.1
Sequence similarities	Belongs to the sirtuin family. Contains 1 deacetylase sirtuin-type domain.

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transit peptide
Ligand	Metal-binding NAD Zinc
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	chromatin silencing Ref.1 Traceable author statement. Source: ProtInc protein amino acid ADP-ribosylation Ref.1 Traceable author statement. Source: ProtInc protein amino acid deacetylation Ref.8 Inferred from direct assay. Source: UniProtKB
Cellular component	mitochondrial intermembrane space Ref.8 Inferred from direct assay. Source: UniProtKB mitochondrial matrix Ref.8 Inferred from direct assay. Source: UniProtKB
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro NAD+ ADP-ribosyltransferase activity Ref.1 Traceable author statement. Source: ProtInc hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NXA8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NXA8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     286-299: RFHFQGPCGTTLPE → SHLISISSLIIIKN
     300-310: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 36

36

Mitochondrion By similarity

Chain

37 – 310

274

NAD-dependent deacetylase sirtuin-5

PRO_0000110266

Regions

Domain

41 – 309

269

Deacetylase sirtuin-type

Nucleotide binding

58 – 77

20

NAD

Nucleotide binding

140 – 144

5

NAD

Nucleotide binding

275 – 276

2

NAD

Sites

Active site

158

1

Proton acceptor

Metal binding

166

1

Zinc

Metal binding

169

1

Zinc

Metal binding

207

1

Zinc

Metal binding

212

1

Zinc

Natural variations

Alternative sequence

286 – 299

14

RFHFQ…TTLPE → SHLISISSLIIIKN in isoform 2.

VSP_008730

Alternative sequence

300 – 310

11

Missing in isoform 2.

VSP_008731

Natural variant

285

1

F → L: dbSNP rs9464003.

VAR_029042

Natural variant

305

1

E → G: dbSNP rs34162626.

VAR_051980

Secondary structure

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310

Helix Strand Turn

Details...

Helix

40 – 49

10

Beta strand

51 – 57

7

Helix

59 – 65

7

Helix

82 – 85

4

Helix

88 – 93

6

Helix

95 – 110

16

Helix

116 – 129

14

Turn

130 – 132

3

Beta strand

134 – 139

6

Helix

145 – 149

5

Beta strand

153 – 156

4

Beta strand

159 – 166

8

Turn

167 – 169

3

Beta strand

172 – 174

3

Beta strand

178 – 181

4

Helix

182 – 184

3

Helix

201 – 203

3

Turn

210 – 212

3

Beta strand

215 – 220

6

Helix

229 – 241

13

Beta strand

243 – 249

7

Helix

257 – 259

3

Helix

260 – 266

7

Beta strand

271 – 277

7

Helix

282 – 284

3

Beta strand

285 – 291

7

Helix

293 – 300

8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 31, 2003. Version 2. Checksum: 022DA32CDB43AC3A	FASTA	310	33,881
	10 20 30 40 50 60 MRPLQIVPSR LISQLYCGLK PPASTRNQIC LKMARPSSSM ADFRKFFAKA KHIVIISGAG 70 80 90 100 110 120 VSAESGVPTF RGAGGYWRKW QAQDLATPLA FAHNPSRVWE FYHYRREVMG SKEPNAGHRA 130 140 150 160 170 180 IAECETRLGK QGRRVVVITQ NIDELHRKAG TKNLLEIHGS LFKTRCTSCG VVAENYKSPI 190 200 210 220 230 240 CPALSGKGAP EPGTQDASIP VEKLPRCEEA GCGGLLRPHV VWFGENLDPA ILEEVDRELA 250 260 270 280 290 300 HCDLCLVVGT SSVVYPAAMF APQVAARGVP VAEFNTETTP ATNRFRFHFQ GPCGTTLPEA 310 LACHENETVS
	Isoform 2. Checksum: 2EE7C311AAA34CBA	FASTA	299	32,674
	10 20 30 40 50 60 MRPLQIVPSR LISQLYCGLK PPASTRNQIC LKMARPSSSM ADFRKFFAKA KHIVIISGAG 70 80 90 100 110 120 VSAESGVPTF RGAGGYWRKW QAQDLATPLA FAHNPSRVWE FYHYRREVMG SKEPNAGHRA 130 140 150 160 170 180 IAECETRLGK QGRRVVVITQ NIDELHRKAG TKNLLEIHGS LFKTRCTSCG VVAENYKSPI 190 200 210 220 230 240 CPALSGKGAP EPGTQDASIP VEKLPRCEEA GCGGLLRPHV VWFGENLDPA ILEEVDRELA 250 260 270 280 290 HCDLCLVVGT SSVVYPAAMF APQVAARGVP VAEFNTETTP ATNRFSHLIS ISSLIIIKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of five human cDNAs with homology to the yeast SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity." Frye R.A. Biochem. Biophys. Res. Commun. 260:273-279(1999) [PubMed: 10381378] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Tissue: Testis.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Hepatoblastoma.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Eye.
[7]	"Evolutionarily conserved and nonconserved cellular localizations and functions of human SIRT proteins." Michishita E., Park J.Y., Burneskis J.M., Barrett J.C., Horikawa I. Mol. Biol. Cell 16:4623-4635(2005) [PubMed: 16079181] [Abstract] Cited for: SUBCELLULAR LOCATION.
[8]	"Substrates and regulation mechanisms for the human mitochondrial sirtuins Sirt3 and Sirt5." Schlicker C., Gertz M., Papatheodorou P., Kachholz B., Becker C.F.W., Steegborn C. J. Mol. Biol. 382:790-801(2008) [PubMed: 18680753] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[9]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[10]	"Crystal structure of human sirtuin homolog 5 in complex with NAD." Structural genomics consortium (SGC) Submitted (FEB-2006) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 34-302 IN COMPLEX WITH NAD AND ZINC IONS.
[11]	"Structural basis of inhibition of the human NAD+-dependent deacetylase SIRT5 by suramin." Schuetz A., Min J., Antoshenko T., Wang C.-L., Allali-Hassani A., Dong A., Loppnau P., Vedadi M., Bochkarev A., Sternglanz R., Plotnikov A.N. Structure 15:377-389(2007) [PubMed: 17355872] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.06 ANGSTROMS) OF 34-302 IN COMPLEXES WITH ZINC IONS; SURAMIN AND ADP-RIBOSE, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF083110 mRNA. Translation: AAD40853.1.
AK000355 mRNA. Translation: BAA91107.1.
AM393414 mRNA. Translation: CAL38292.1.
AL441883 Genomic DNA. Translation: CAI19837.1.
AL441883 Genomic DNA. Translation: CAI19838.1.
CH471087 Genomic DNA. Translation: EAW55332.1.
BC000126 mRNA. Translation: AAH00126.1.

IPI

IPI00010331.
IPI00016807.

RefSeq

NP_036373.1.
NP_112534.1.

UniGene

Hs.567431
Hs.594133

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2B4Y	X-ray	1.90	A/B/C/D	34-302	[»]
2NYR	X-ray	2.06	A/B	34-302	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

Q9NXA8.

Proteomic databases

PRIDE

Q9NXA8.

Genome annotation databases

Ensembl

ENST00000379250; ENSP00000368552; ENSG00000124523; Homo sapiens. [Genome view]

GeneID

23408.

KEGG

hsa:23408.

NMPDR

fig|9606.3.peg.26474.

UCSC

uc003naw.1. human.
uc003nay.1. human.

Organism-specific databases

CTD

23408.

GeneCards

GC06P013682.

H-InvDB

HIX0005588.

HGNC

HGNC:14933. SIRT5.

HPA

HPA021798.
HPA022002.
HPA022992.

MIM

604483. gene.

PharmGKB

PA37938.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG18556.

HOGENOM

HBG641281.

HOVERGEN

Q9NXA8.

InParanoid

Q9NXA8.

OMA

FVHEARL.

OrthoDB

EOG9CVJS7.

PhylomeDB

Q9NXA8.

Enzyme and pathway databases

Pathway_Interaction_DB

hdac_classi_pathway. Signaling events mediated by HDAC Class I.

Gene expression databases

ArrayExpress

Q9NXA8.

Bgee

Q9NXA8.

CleanEx

HS_SIRT5.

Genevestigator

Q9NXA8.

GermOnline

ENSG00000124523. Homo sapiens.

Family and domain databases

InterPro

IPR003000. NAD-dep_histone_deAcase_SIR2.
[Graphical view]

PANTHER

PTHR11085. SIR2. 1 hit.

Pfam

PF02146. SIR2. 1 hit.
[Graphical view]

PROSITE

PS50305. SIRTUIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

DrugBank

DB04786. Suramin.

NextBio

45587.

SOURCE

Search...

Entry information

Entry name

SIRT5_HUMAN

Accession

Primary (citable) accession number: Q9NXA8
Secondary accession number(s): Q5T294, Q5T295, Q9Y6E6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	October 31, 2003
Last modified:	January 19, 2010
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.