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Protein

tRNA-dihydrouridine(20) synthase [NAD(P)+]-like

Gene

DUS2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Dihydrouridine synthase. Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Negatively regulates the activation of EIF2AK2/PKR.2 Publications

Cofactori

FMNBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87FMNBy similarity1
Active sitei116Proton donorBy similarity1
Binding sitei155FMNBy similarity1
Binding sitei183FMNBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 20FMNBy similarity3
Nucleotide bindingi214 – 216FMNBy similarity3
Nucleotide bindingi242 – 243FMNBy similarity2

GO - Molecular functioni

  • double-stranded RNA binding Source: UniProtKB
  • flavin adenine dinucleotide binding Source: InterPro
  • protein kinase inhibitor activity Source: UniProtKB
  • tRNA dihydrouridine synthase activity Source: GO_Central

GO - Biological processi

  • negative regulation of cell death Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase, RNA-binding
Biological processtRNA processing
LigandFlavoprotein, FMN

Enzyme and pathway databases

BRENDAi1.3.1.91 2681
ReactomeiR-HSA-6782315 tRNA modification in the nucleus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-dihydrouridine(20) synthase [NAD(P)+]-like (EC:1.3.1.-)
Alternative name(s):
Dihydrouridine synthase 2
Up-regulated in lung cancer protein 8
Short name:
URLC8
tRNA-dihydrouridine synthase 2-like
Short name:
hDUS2
Gene namesi
Name:DUS2
Synonyms:DUS2L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000167264.17
HGNCiHGNC:26014 DUS2
MIMi609707 gene
neXtProtiNX_Q9NX74

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum

Pathology & Biotechi

Organism-specific databases

DisGeNETi54920
OpenTargetsiENSG00000167264
PharmGKBiPA142671937

Polymorphism and mutation databases

DMDMi73620832

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001621571 – 493tRNA-dihydrouridine(20) synthase [NAD(P)+]-likeAdd BLAST493

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei445PhosphoserineCombined sources1
Modified residuei488PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9NX74
MaxQBiQ9NX74
PaxDbiQ9NX74
PeptideAtlasiQ9NX74
PRIDEiQ9NX74

PTM databases

iPTMnetiQ9NX74
PhosphoSitePlusiQ9NX74

Expressioni

Tissue specificityi

Weak expression in heart, placenta and skeletal muscle. Up-regulated in most lung cancer cells (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000167264
CleanExiHS_DUS2L
ExpressionAtlasiQ9NX74 baseline and differential
GenevisibleiQ9NX74 HS

Organism-specific databases

HPAiHPA042560
HPA043528

Interactioni

Subunit structurei

Interacts with EPRS. Interacts (via DRBM domain) with PRKRA and EIF2AK2/PKR (via DRBM 1 domain).2 Publications

Protein-protein interaction databases

BioGridi120261, 4 interactors
IntActiQ9NX74, 11 interactors
MINTiQ9NX74
STRINGi9606.ENSP00000351769

Structurei

Secondary structure

1493
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 16Combined sources3
Turni20 – 23Combined sources4
Helixi25 – 33Combined sources9
Beta strandi37 – 40Combined sources4
Helixi46 – 49Combined sources4
Beta strandi53 – 57Combined sources5
Turni58 – 61Combined sources4
Beta strandi62 – 66Combined sources5
Beta strandi72 – 76Combined sources5
Helixi78 – 80Combined sources3
Turni81 – 83Combined sources3
Beta strandi84 – 89Combined sources6
Helixi93 – 103Combined sources11
Helixi104 – 106Combined sources3
Beta strandi108 – 113Combined sources6
Helixi130 – 132Combined sources3
Helixi134 – 147Combined sources14
Beta strandi152 – 158Combined sources7
Helixi162 – 173Combined sources12
Turni174 – 176Combined sources3
Beta strandi178 – 186Combined sources9
Helixi197 – 206Combined sources10
Beta strandi211 – 214Combined sources4
Turni218 – 220Combined sources3
Beta strandi221 – 223Combined sources3
Helixi224 – 234Combined sources11
Beta strandi237 – 242Combined sources6
Helixi243 – 247Combined sources5
Helixi249 – 252Combined sources4
Helixi260 – 273Combined sources14
Helixi278 – 288Combined sources11
Turni289 – 291Combined sources3
Helixi296 – 303Combined sources8
Helixi307 – 313Combined sources7
Helixi317 – 333Combined sources17
Helixi336 – 338Combined sources3
Beta strandi351 – 355Combined sources5
Helixi361 – 363Combined sources3
Helixi370 – 380Combined sources11
Beta strandi387 – 393Combined sources7
Helixi394 – 396Combined sources3
Beta strandi398 – 405Combined sources8
Beta strandi408 – 414Combined sources7
Beta strandi416 – 418Combined sources3
Helixi419 – 433Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4WFSX-ray2.68A14-333[»]
4WFTX-ray1.70A/B/C338-450[»]
4XP7X-ray1.90A1-340[»]
ProteinModelPortaliQ9NX74
SMRiQ9NX74
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini369 – 436DRBMAdd BLAST68

Sequence similaritiesi

Belongs to the Dus family. Dus2 subfamily.Curated

Phylogenomic databases

eggNOGiKOG2334 Eukaryota
COG0042 LUCA
GeneTreeiENSGT00550000075019
HOGENOMiHOG000195580
HOVERGENiHBG079551
InParanoidiQ9NX74
KOiK05543
OMAiTSGVIKM
OrthoDBiEOG091G0CCW
PhylomeDBiQ9NX74
TreeFamiTF106151

Family and domain databases

CDDicd02801 DUS_like_FMN, 1 hit
Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR014720 dsRBD_dom
IPR035587 DUS-like_FMN-bd
IPR001269 tRNA_hU_synthase
IPR018517 tRNA_hU_synthase_CS
PANTHERiPTHR11082 PTHR11082, 1 hit
PfamiView protein in Pfam
PF00035 dsrm, 1 hit
PF01207 Dus, 1 hit
SMARTiView protein in SMART
SM00358 DSRM, 1 hit
PROSITEiView protein in PROSITE
PS01136 UPF0034, 1 hit

Sequencei

Sequence statusi: Complete.

Q9NX74-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILNSLSLCY HNKLILAPMV RVGTLPMRLL ALDYGADIVY CEELIDLKMI
60 70 80 90 100
QCKRVVNEVL STVDFVAPDD RVVFRTCERE QNRVVFQMGT SDAERALAVA
110 120 130 140 150
RLVENDVAGI DVNMGCPKQY STKGGMGAAL LSDPDKIEKI LSTLVKGTRR
160 170 180 190 200
PVTCKIRILP SLEDTLSLVK RIERTGIAAI AVHGRKREER PQHPVSCEVI
210 220 230 240 250
KAIADTLSIP VIANGGSHDH IQQYSDIEDF RQATAASSVM VARAAMWNPS
260 270 280 290 300
IFLKEGLRPL EEVMQKYIRY AVQYDNHYTN TKYCLCQMLR EQLESPQGRL
310 320 330 340 350
LHAAQSSREI CEAFGLGAFY EETTQELDAQ QARLSAKTSE QTGEPAEDTS
360 370 380 390 400
GVIKMAVKFD RRAYPAQITP KMCLLEWCRR EKLAQPVYET VQRPLDRLFS
410 420 430 440 450
SIVTVAEQKY QSTLWDKSKK LAEQAAAIVC LRSQGLPEGR LGEESPSLHK
460 470 480 490
RKREAPDQDP GGPRAQELAQ PGDLCKKPFV ALGSGEESPL EGW
Length:493
Mass (Da):55,050
Last modified:October 1, 2000 - v1
Checksum:i8CFE5046CCF79DCD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB101210 mRNA Translation: BAE07219.1
AK000406 mRNA Translation: BAA91143.1
AK290578 mRNA Translation: BAF83267.1
AC130462 Genomic DNA No translation available.
CH471092 Genomic DNA Translation: EAW83207.1
BC006527 mRNA Translation: AAH06527.1
CCDSiCCDS10859.1
RefSeqiNP_001258691.1, NM_001271762.1
NP_001258692.1, NM_001271763.1
NP_060273.1, NM_017803.4
UniGeneiHs.534460
Hs.744492

Genome annotation databases

EnsembliENST00000358896; ENSP00000351769; ENSG00000167264
ENST00000565263; ENSP00000455229; ENSG00000167264
GeneIDi54920
KEGGihsa:54920
UCSCiuc002evi.5 human

Similar proteinsi

Entry informationi

Entry nameiDUS2L_HUMAN
AccessioniPrimary (citable) accession number: Q9NX74
Secondary accession number(s): A8K3G3, Q4H4D9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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