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Q9NX74

- DUS2L_HUMAN

UniProt

Q9NX74 - DUS2L_HUMAN

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Protein

tRNA-dihydrouridine(20) synthase [NAD(P)+]-like

Gene

DUS2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Dihydrouridine synthase. Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Negatively regulates the activation of EIF2AK2/PKR.2 Publications

Cofactori

FAD.By similarity

GO - Molecular functioni

  1. double-stranded RNA binding Source: UniProtKB
  2. flavin adenine dinucleotide binding Source: InterPro
  3. protein kinase inhibitor activity Source: UniProtKB
  4. tRNA dihydrouridine synthase activity Source: InterPro

GO - Biological processi

  1. negative regulation of cell death Source: UniProtKB
  2. negative regulation of protein kinase activity Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-dihydrouridine(20) synthase [NAD(P)+]-like (EC:1.3.1.-)
Alternative name(s):
Dihydrouridine synthase 2
Up-regulated in lung cancer protein 8
Short name:
URLC8
tRNA-dihydrouridine synthase 2-like
Short name:
hDUS2
Gene namesi
Name:DUS2
Synonyms:DUS2L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 16

Organism-specific databases

HGNCiHGNC:26014. DUS2.

Subcellular locationi

Cytoplasm 1 Publication. Endoplasmic reticulum 1 Publication
Note: Mainly at the endoplasmic reticulum.

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. endoplasmic reticulum Source: UniProtKB-KW
  3. mitochondrion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671937.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 493493tRNA-dihydrouridine(20) synthase [NAD(P)+]-likePRO_0000162157Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei488 – 4881Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9NX74.
PaxDbiQ9NX74.
PRIDEiQ9NX74.

PTM databases

PhosphoSiteiQ9NX74.

Expressioni

Tissue specificityi

Weak expression in heart, placenta and skeletal muscle. Up-regulated in most lung cancer cells (at protein level).1 Publication

Gene expression databases

BgeeiQ9NX74.
CleanExiHS_DUS2L.
ExpressionAtlasiQ9NX74. baseline and differential.
GenevestigatoriQ9NX74.

Organism-specific databases

HPAiHPA042560.
HPA043528.

Interactioni

Subunit structurei

Interacts with EPRS. Interacts (via DRBM domain) with PRKRA and EIF2AK2/PKR (via DRBM 1 domain).2 Publications

Protein-protein interaction databases

BioGridi120261. 4 interactions.
IntActiQ9NX74. 3 interactions.
MINTiMINT-3076069.
STRINGi9606.ENSP00000351769.

Structurei

3D structure databases

ProteinModelPortaliQ9NX74.
SMRiQ9NX74. Positions 13-270, 352-465.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini369 – 43668DRBMAdd
BLAST

Sequence similaritiesi

Belongs to the Dus family. Dus2 subfamily.Curated

Phylogenomic databases

eggNOGiCOG0042.
GeneTreeiENSGT00550000075019.
HOGENOMiHOG000195580.
HOVERGENiHBG079551.
InParanoidiQ9NX74.
KOiK05543.
OMAiEDTSGVI.
PhylomeDBiQ9NX74.
TreeFamiTF106151.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.30.160.20. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR014720. dsRNA-bd_dom.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
[Graphical view]
PANTHERiPTHR11082. PTHR11082. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF01207. Dus. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
[Graphical view]
PROSITEiPS01136. UPF0034. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9NX74-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MILNSLSLCY HNKLILAPMV RVGTLPMRLL ALDYGADIVY CEELIDLKMI
60 70 80 90 100
QCKRVVNEVL STVDFVAPDD RVVFRTCERE QNRVVFQMGT SDAERALAVA
110 120 130 140 150
RLVENDVAGI DVNMGCPKQY STKGGMGAAL LSDPDKIEKI LSTLVKGTRR
160 170 180 190 200
PVTCKIRILP SLEDTLSLVK RIERTGIAAI AVHGRKREER PQHPVSCEVI
210 220 230 240 250
KAIADTLSIP VIANGGSHDH IQQYSDIEDF RQATAASSVM VARAAMWNPS
260 270 280 290 300
IFLKEGLRPL EEVMQKYIRY AVQYDNHYTN TKYCLCQMLR EQLESPQGRL
310 320 330 340 350
LHAAQSSREI CEAFGLGAFY EETTQELDAQ QARLSAKTSE QTGEPAEDTS
360 370 380 390 400
GVIKMAVKFD RRAYPAQITP KMCLLEWCRR EKLAQPVYET VQRPLDRLFS
410 420 430 440 450
SIVTVAEQKY QSTLWDKSKK LAEQAAAIVC LRSQGLPEGR LGEESPSLHK
460 470 480 490
RKREAPDQDP GGPRAQELAQ PGDLCKKPFV ALGSGEESPL EGW
Length:493
Mass (Da):55,050
Last modified:October 1, 2000 - v1
Checksum:i8CFE5046CCF79DCD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB101210 mRNA. Translation: BAE07219.1.
AK000406 mRNA. Translation: BAA91143.1.
AK290578 mRNA. Translation: BAF83267.1.
AC130462 Genomic DNA. No translation available.
CH471092 Genomic DNA. Translation: EAW83207.1.
BC006527 mRNA. Translation: AAH06527.1.
CCDSiCCDS10859.1.
RefSeqiNP_001258691.1. NM_001271762.1.
NP_001258692.1. NM_001271763.1.
NP_060273.1. NM_017803.4.
UniGeneiHs.534460.
Hs.744492.

Genome annotation databases

EnsembliENST00000358896; ENSP00000351769; ENSG00000167264.
ENST00000565263; ENSP00000455229; ENSG00000167264.
GeneIDi54920.
KEGGihsa:54920.
UCSCiuc002evi.4. human.

Polymorphism databases

DMDMi73620832.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB101210 mRNA. Translation: BAE07219.1 .
AK000406 mRNA. Translation: BAA91143.1 .
AK290578 mRNA. Translation: BAF83267.1 .
AC130462 Genomic DNA. No translation available.
CH471092 Genomic DNA. Translation: EAW83207.1 .
BC006527 mRNA. Translation: AAH06527.1 .
CCDSi CCDS10859.1.
RefSeqi NP_001258691.1. NM_001271762.1.
NP_001258692.1. NM_001271763.1.
NP_060273.1. NM_017803.4.
UniGenei Hs.534460.
Hs.744492.

3D structure databases

ProteinModelPortali Q9NX74.
SMRi Q9NX74. Positions 13-270, 352-465.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 120261. 4 interactions.
IntActi Q9NX74. 3 interactions.
MINTi MINT-3076069.
STRINGi 9606.ENSP00000351769.

PTM databases

PhosphoSitei Q9NX74.

Polymorphism databases

DMDMi 73620832.

Proteomic databases

MaxQBi Q9NX74.
PaxDbi Q9NX74.
PRIDEi Q9NX74.

Protocols and materials databases

DNASUi 54920.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000358896 ; ENSP00000351769 ; ENSG00000167264 .
ENST00000565263 ; ENSP00000455229 ; ENSG00000167264 .
GeneIDi 54920.
KEGGi hsa:54920.
UCSCi uc002evi.4. human.

Organism-specific databases

CTDi 54920.
GeneCardsi GC16P068021.
HGNCi HGNC:26014. DUS2.
HPAi HPA042560.
HPA043528.
MIMi 609707. gene.
neXtProti NX_Q9NX74.
PharmGKBi PA142671937.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0042.
GeneTreei ENSGT00550000075019.
HOGENOMi HOG000195580.
HOVERGENi HBG079551.
InParanoidi Q9NX74.
KOi K05543.
OMAi EDTSGVI.
PhylomeDBi Q9NX74.
TreeFami TF106151.

Miscellaneous databases

GenomeRNAii 54920.
NextBioi 57992.
PROi Q9NX74.
SOURCEi Search...

Gene expression databases

Bgeei Q9NX74.
CleanExi HS_DUS2L.
ExpressionAtlasi Q9NX74. baseline and differential.
Genevestigatori Q9NX74.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
3.30.160.20. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR014720. dsRNA-bd_dom.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
[Graphical view ]
PANTHERi PTHR11082. PTHR11082. 1 hit.
Pfami PF00035. dsrm. 1 hit.
PF01207. Dus. 1 hit.
[Graphical view ]
SMARTi SM00358. DSRM. 1 hit.
[Graphical view ]
PROSITEi PS01136. UPF0034. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel human tRNA-dihydrouridine synthase involved in pulmonary carcinogenesis."
    Kato T., Daigo Y., Hayama S., Ishikawa N., Yamabuki T., Ito T., Miyamoto M., Kondo S., Nakamura Y.
    Cancer Res. 65:5638-5646(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH EPRS.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Gastric carcinoma.
  3. "The sequence and analysis of duplication-rich human chromosome 16."
    Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
    , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
    Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Colon.
  6. "Interaction of human tRNA-dihydrouridine synthase-2 with interferon-induced protein kinase PKR."
    Mittelstadt M., Frump A., Khuu T., Fowlkes V., Handy I., Patel C.V., Patel R.C.
    Nucleic Acids Res. 36:998-1008(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PRKRA AND EIF2AK2.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-488, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-488, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDUS2L_HUMAN
AccessioniPrimary (citable) accession number: Q9NX74
Secondary accession number(s): A8K3G3, Q4H4D9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3