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Protein

tRNA-dihydrouridine(20) synthase [NAD(P)+]-like

Gene

DUS2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dihydrouridine synthase. Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Negatively regulates the activation of EIF2AK2/PKR.2 Publications

Cofactori

FMNBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87FMNBy similarity1
Active sitei116Proton donorBy similarity1
Binding sitei155FMNBy similarity1
Binding sitei183FMNBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 20FMNBy similarity3
Nucleotide bindingi214 – 216FMNBy similarity3
Nucleotide bindingi242 – 243FMNBy similarity2

GO - Molecular functioni

  • double-stranded RNA binding Source: UniProtKB
  • flavin adenine dinucleotide binding Source: InterPro
  • protein kinase inhibitor activity Source: UniProtKB
  • tRNA dihydrouridine synthase activity Source: GO_Central

GO - Biological processi

  • negative regulation of cell death Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Flavoprotein, FMN, RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000167264-MONOMER.
BRENDAi1.3.1.91. 2681.
ReactomeiR-HSA-6782315. tRNA modification in the nucleus and cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-dihydrouridine(20) synthase [NAD(P)+]-like (EC:1.3.1.-)
Alternative name(s):
Dihydrouridine synthase 2
Up-regulated in lung cancer protein 8
Short name:
URLC8
tRNA-dihydrouridine synthase 2-like
Short name:
hDUS2
Gene namesi
Name:DUS2
Synonyms:DUS2L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:26014. DUS2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum

Pathology & Biotechi

Organism-specific databases

DisGeNETi54920.
OpenTargetsiENSG00000167264.
PharmGKBiPA142671937.

Polymorphism and mutation databases

DMDMi73620832.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001621571 – 493tRNA-dihydrouridine(20) synthase [NAD(P)+]-likeAdd BLAST493

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei445PhosphoserineCombined sources1
Modified residuei488PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9NX74.
MaxQBiQ9NX74.
PaxDbiQ9NX74.
PeptideAtlasiQ9NX74.
PRIDEiQ9NX74.

PTM databases

iPTMnetiQ9NX74.
PhosphoSitePlusiQ9NX74.

Expressioni

Tissue specificityi

Weak expression in heart, placenta and skeletal muscle. Up-regulated in most lung cancer cells (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000167264.
CleanExiHS_DUS2L.
ExpressionAtlasiQ9NX74. baseline and differential.
GenevisibleiQ9NX74. HS.

Organism-specific databases

HPAiHPA042560.
HPA043528.

Interactioni

Subunit structurei

Interacts with EPRS. Interacts (via DRBM domain) with PRKRA and EIF2AK2/PKR (via DRBM 1 domain).2 Publications

Protein-protein interaction databases

BioGridi120261. 4 interactors.
IntActiQ9NX74. 11 interactors.
MINTiMINT-3076069.
STRINGi9606.ENSP00000351769.

Structurei

Secondary structure

1493
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 16Combined sources3
Turni20 – 23Combined sources4
Helixi25 – 33Combined sources9
Beta strandi37 – 40Combined sources4
Helixi46 – 49Combined sources4
Beta strandi53 – 57Combined sources5
Turni58 – 61Combined sources4
Beta strandi62 – 66Combined sources5
Beta strandi72 – 76Combined sources5
Helixi78 – 80Combined sources3
Turni81 – 83Combined sources3
Beta strandi84 – 89Combined sources6
Helixi93 – 103Combined sources11
Helixi104 – 106Combined sources3
Beta strandi108 – 113Combined sources6
Helixi130 – 132Combined sources3
Helixi134 – 147Combined sources14
Beta strandi152 – 158Combined sources7
Helixi162 – 173Combined sources12
Turni174 – 176Combined sources3
Beta strandi178 – 186Combined sources9
Helixi197 – 206Combined sources10
Beta strandi211 – 214Combined sources4
Turni218 – 220Combined sources3
Beta strandi221 – 223Combined sources3
Helixi224 – 234Combined sources11
Beta strandi237 – 242Combined sources6
Helixi243 – 247Combined sources5
Helixi249 – 252Combined sources4
Helixi260 – 273Combined sources14
Helixi278 – 288Combined sources11
Turni289 – 291Combined sources3
Helixi296 – 303Combined sources8
Helixi307 – 313Combined sources7
Helixi317 – 333Combined sources17
Helixi336 – 338Combined sources3
Beta strandi351 – 355Combined sources5
Helixi361 – 363Combined sources3
Helixi370 – 380Combined sources11
Beta strandi387 – 393Combined sources7
Helixi394 – 396Combined sources3
Beta strandi398 – 405Combined sources8
Beta strandi408 – 414Combined sources7
Beta strandi416 – 418Combined sources3
Helixi419 – 433Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4WFSX-ray2.68A14-333[»]
4WFTX-ray1.70A/B/C338-450[»]
4XP7X-ray1.90A1-340[»]
ProteinModelPortaliQ9NX74.
SMRiQ9NX74.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini369 – 436DRBMAdd BLAST68

Sequence similaritiesi

Belongs to the Dus family. Dus2 subfamily.Curated

Phylogenomic databases

eggNOGiKOG2334. Eukaryota.
COG0042. LUCA.
GeneTreeiENSGT00550000075019.
HOGENOMiHOG000195580.
HOVERGENiHBG079551.
InParanoidiQ9NX74.
KOiK05543.
OMAiTSGVIKM.
OrthoDBiEOG091G0CCW.
PhylomeDBiQ9NX74.
TreeFamiTF106151.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.30.160.20. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR014720. dsRBD_dom.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
[Graphical view]
PANTHERiPTHR11082. PTHR11082. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF01207. Dus. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
[Graphical view]
PROSITEiPS01136. UPF0034. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9NX74-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILNSLSLCY HNKLILAPMV RVGTLPMRLL ALDYGADIVY CEELIDLKMI
60 70 80 90 100
QCKRVVNEVL STVDFVAPDD RVVFRTCERE QNRVVFQMGT SDAERALAVA
110 120 130 140 150
RLVENDVAGI DVNMGCPKQY STKGGMGAAL LSDPDKIEKI LSTLVKGTRR
160 170 180 190 200
PVTCKIRILP SLEDTLSLVK RIERTGIAAI AVHGRKREER PQHPVSCEVI
210 220 230 240 250
KAIADTLSIP VIANGGSHDH IQQYSDIEDF RQATAASSVM VARAAMWNPS
260 270 280 290 300
IFLKEGLRPL EEVMQKYIRY AVQYDNHYTN TKYCLCQMLR EQLESPQGRL
310 320 330 340 350
LHAAQSSREI CEAFGLGAFY EETTQELDAQ QARLSAKTSE QTGEPAEDTS
360 370 380 390 400
GVIKMAVKFD RRAYPAQITP KMCLLEWCRR EKLAQPVYET VQRPLDRLFS
410 420 430 440 450
SIVTVAEQKY QSTLWDKSKK LAEQAAAIVC LRSQGLPEGR LGEESPSLHK
460 470 480 490
RKREAPDQDP GGPRAQELAQ PGDLCKKPFV ALGSGEESPL EGW
Length:493
Mass (Da):55,050
Last modified:October 1, 2000 - v1
Checksum:i8CFE5046CCF79DCD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB101210 mRNA. Translation: BAE07219.1.
AK000406 mRNA. Translation: BAA91143.1.
AK290578 mRNA. Translation: BAF83267.1.
AC130462 Genomic DNA. No translation available.
CH471092 Genomic DNA. Translation: EAW83207.1.
BC006527 mRNA. Translation: AAH06527.1.
CCDSiCCDS10859.1.
RefSeqiNP_001258691.1. NM_001271762.1.
NP_001258692.1. NM_001271763.1.
NP_060273.1. NM_017803.4.
UniGeneiHs.534460.
Hs.744492.

Genome annotation databases

EnsembliENST00000358896; ENSP00000351769; ENSG00000167264.
ENST00000565263; ENSP00000455229; ENSG00000167264.
GeneIDi54920.
KEGGihsa:54920.
UCSCiuc002evi.5. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB101210 mRNA. Translation: BAE07219.1.
AK000406 mRNA. Translation: BAA91143.1.
AK290578 mRNA. Translation: BAF83267.1.
AC130462 Genomic DNA. No translation available.
CH471092 Genomic DNA. Translation: EAW83207.1.
BC006527 mRNA. Translation: AAH06527.1.
CCDSiCCDS10859.1.
RefSeqiNP_001258691.1. NM_001271762.1.
NP_001258692.1. NM_001271763.1.
NP_060273.1. NM_017803.4.
UniGeneiHs.534460.
Hs.744492.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4WFSX-ray2.68A14-333[»]
4WFTX-ray1.70A/B/C338-450[»]
4XP7X-ray1.90A1-340[»]
ProteinModelPortaliQ9NX74.
SMRiQ9NX74.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120261. 4 interactors.
IntActiQ9NX74. 11 interactors.
MINTiMINT-3076069.
STRINGi9606.ENSP00000351769.

PTM databases

iPTMnetiQ9NX74.
PhosphoSitePlusiQ9NX74.

Polymorphism and mutation databases

DMDMi73620832.

Proteomic databases

EPDiQ9NX74.
MaxQBiQ9NX74.
PaxDbiQ9NX74.
PeptideAtlasiQ9NX74.
PRIDEiQ9NX74.

Protocols and materials databases

DNASUi54920.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000358896; ENSP00000351769; ENSG00000167264.
ENST00000565263; ENSP00000455229; ENSG00000167264.
GeneIDi54920.
KEGGihsa:54920.
UCSCiuc002evi.5. human.

Organism-specific databases

CTDi54920.
DisGeNETi54920.
GeneCardsiDUS2.
HGNCiHGNC:26014. DUS2.
HPAiHPA042560.
HPA043528.
MIMi609707. gene.
neXtProtiNX_Q9NX74.
OpenTargetsiENSG00000167264.
PharmGKBiPA142671937.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2334. Eukaryota.
COG0042. LUCA.
GeneTreeiENSGT00550000075019.
HOGENOMiHOG000195580.
HOVERGENiHBG079551.
InParanoidiQ9NX74.
KOiK05543.
OMAiTSGVIKM.
OrthoDBiEOG091G0CCW.
PhylomeDBiQ9NX74.
TreeFamiTF106151.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000167264-MONOMER.
BRENDAi1.3.1.91. 2681.
ReactomeiR-HSA-6782315. tRNA modification in the nucleus and cytosol.

Miscellaneous databases

GenomeRNAii54920.
PROiQ9NX74.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000167264.
CleanExiHS_DUS2L.
ExpressionAtlasiQ9NX74. baseline and differential.
GenevisibleiQ9NX74. HS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.30.160.20. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR014720. dsRBD_dom.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
[Graphical view]
PANTHERiPTHR11082. PTHR11082. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF01207. Dus. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
[Graphical view]
PROSITEiPS01136. UPF0034. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDUS2L_HUMAN
AccessioniPrimary (citable) accession number: Q9NX74
Secondary accession number(s): A8K3G3, Q4H4D9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.