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Protein

Mediator of RNA polymerase II transcription subunit 29

Gene

MED29

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_116145. PPARA activates gene expression.
REACT_27161. Transcriptional regulation of white adipocyte differentiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 29
Alternative name(s):
Intersex-like protein
Mediator complex subunit 29
Gene namesi
Name:MED29
Synonyms:IXL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:23074. MED29.

Subcellular locationi

GO - Cellular componenti

  • mediator complex Source: MGI
  • nucleoplasm Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162395655.

Polymorphism and mutation databases

DMDMi74734688.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 200199Mediator of RNA polymerase II transcription subunit 29PRO_0000288058Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9NX70.
PaxDbiQ9NX70.
PeptideAtlasiQ9NX70.
PRIDEiQ9NX70.

PTM databases

PhosphoSiteiQ9NX70.

Expressioni

Tissue specificityi

Widely expressed in embryo and adult.1 Publication

Gene expression databases

BgeeiQ9NX70.
CleanExiHS_MED29.
ExpressionAtlasiQ9NX70. baseline and differential.
GenevisibleiQ9NX70. HS.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Associates with the MED18/MED20 heteromer.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Med11Q9D8C62EBI-394656,EBI-6260909From a different organism.
MED18Q9BUE05EBI-394656,EBI-394640
MED19A0JLT28EBI-394656,EBI-394430
MED20Q9H9445EBI-394656,EBI-394644
Med20Q9R0X02EBI-394656,EBI-398698From a different organism.
MED22Q155284EBI-394656,EBI-394687
MED28Q9H2043EBI-394656,EBI-514199
Med30Q9CQI92EBI-394656,EBI-309220From a different organism.
MED6O755863EBI-394656,EBI-394624
Med8Q9D7W52EBI-394656,EBI-7990252From a different organism.

Protein-protein interaction databases

BioGridi120734. 56 interactions.
IntActiQ9NX70. 87 interactions.
MINTiMINT-275786.
STRINGi9606.ENSP00000314343.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi29 – 8456Gln-richAdd
BLAST

Sequence similaritiesi

Belongs to the Mediator complex subunit 29 family.Curated

Phylogenomic databases

eggNOGiNOG48023.
GeneTreeiENSGT00390000007540.
HOVERGENiHBG060489.
InParanoidiQ9NX70.
KOiK15142.
PhylomeDBiQ9NX70.
TreeFamiTF326632.

Family and domain databases

InterProiIPR021018. Mediator_Med29_met.
[Graphical view]
PfamiPF11568. Med29. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NX70-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAASQQQASA ASSAAGVSGP SSAGGPGPQQ QPQPPAQLVG PAQSGLLQQQ
60 70 80 90 100
QQDFDPVQRY KMLIPQLKES LQTLMKVAAQ NLIQNTNIDN GQKSSDGPIQ
110 120 130 140 150
RFDKCLEEFY ALCDQLELCL RLAHECLSQS CDSAKHSPTL VPTATKPDAV
160 170 180 190 200
QPDSLPYPQY LAVIKAQISC AKDIHTALLD CANKVTGKTP APPAGPGGTL
Length:200
Mass (Da):21,073
Last modified:October 1, 2000 - v1
Checksum:iE0CDA1DDD89635CA
GO
Isoform 2 (identifier: Q9NX70-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     121-196: RLAHECLSQS...GKTPAPPAGP → PPSPTQCSLT...QWLVGGVQRE
     197-200: Missing.

Note: No experimental confirmation available.
Show »
Length:196
Mass (Da):21,362
Checksum:i5A3367EFACBD5356
GO

Sequence cautioni

The sequence AAU43732.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei121 – 19676RLAHE…PPAGP → PPSPTQCSLTASPTHSTWRS SKPRFPVPRTFTPPCWTVPT RSRARHPHHLLALGALCEVG DREWGRQWLVGGVQRE in isoform 2. 1 PublicationVSP_056133Add
BLAST
Alternative sequencei197 – 2004Missing in isoform 2. 1 PublicationVSP_056134

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY729650 mRNA. Translation: AAU43732.1. Different initiation.
AK000411 mRNA. Translation: BAA91147.1.
AK298014 mRNA. Translation: BAG60318.1.
AL137304 mRNA. Translation: CAB70687.2.
AC005239 Genomic DNA. No translation available.
AC011500 Genomic DNA. No translation available.
AC104106 Genomic DNA. No translation available.
BC019015 mRNA. Translation: AAH19015.1.
PIRiT46497.
RefSeqiNP_060062.1. NM_017592.1.
UniGeneiHs.611541.

Genome annotation databases

EnsembliENST00000594368; ENSP00000472501; ENSG00000063322. [Q9NX70-2]
ENST00000599213; ENSP00000471802; ENSG00000063322. [Q9NX70-1]
GeneIDi55588.
KEGGihsa:55588.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY729650 mRNA. Translation: AAU43732.1. Different initiation.
AK000411 mRNA. Translation: BAA91147.1.
AK298014 mRNA. Translation: BAG60318.1.
AL137304 mRNA. Translation: CAB70687.2.
AC005239 Genomic DNA. No translation available.
AC011500 Genomic DNA. No translation available.
AC104106 Genomic DNA. No translation available.
BC019015 mRNA. Translation: AAH19015.1.
PIRiT46497.
RefSeqiNP_060062.1. NM_017592.1.
UniGeneiHs.611541.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120734. 56 interactions.
IntActiQ9NX70. 87 interactions.
MINTiMINT-275786.
STRINGi9606.ENSP00000314343.

PTM databases

PhosphoSiteiQ9NX70.

Polymorphism and mutation databases

DMDMi74734688.

Proteomic databases

MaxQBiQ9NX70.
PaxDbiQ9NX70.
PeptideAtlasiQ9NX70.
PRIDEiQ9NX70.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000594368; ENSP00000472501; ENSG00000063322. [Q9NX70-2]
ENST00000599213; ENSP00000471802; ENSG00000063322. [Q9NX70-1]
GeneIDi55588.
KEGGihsa:55588.

Organism-specific databases

CTDi55588.
GeneCardsiGC19P039881.
H-InvDBHIX0015117.
HGNCiHGNC:23074. MED29.
MIMi612914. gene.
neXtProtiNX_Q9NX70.
PharmGKBiPA162395655.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG48023.
GeneTreeiENSGT00390000007540.
HOVERGENiHBG060489.
InParanoidiQ9NX70.
KOiK15142.
PhylomeDBiQ9NX70.
TreeFamiTF326632.

Enzyme and pathway databases

ReactomeiREACT_116145. PPARA activates gene expression.
REACT_27161. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

ChiTaRSiMED29. human.
GenomeRNAii55588.
NextBioi35473673.
PROiQ9NX70.
SOURCEiSearch...

Gene expression databases

BgeeiQ9NX70.
CleanExiHS_MED29.
ExpressionAtlasiQ9NX70. baseline and differential.
GenevisibleiQ9NX70. HS.

Family and domain databases

InterProiIPR021018. Mediator_Med29_met.
[Graphical view]
PfamiPF11568. Med29. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "IXL, a new subunit of the mammalian Mediator complex, functions as a transcriptional suppressor."
    Wang Y., Li Y., Zeng W., Zhu C., Xiao J., Yuan W., Wang Y., Cai Z., Zhou J., Liu M., Wu X.
    Biochem. Biophys. Res. Commun. 325:1330-1338(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Tissue: Embryonic heart.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Lung and Signet-ring cell carcinoma.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Testis.
  4. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  6. "A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex."
    Sato S., Tomomori-Sato C., Banks C.A.S., Parmely T.J., Sorokina I., Brower C.S., Conaway R.C., Conaway J.W.
    J. Biol. Chem. 278:49671-49674(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE TRAP/SMCC MEDIATOR COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH MED18 AND MED20.
  7. "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2."
    Tomomori-Sato C., Sato S., Parmely T.J., Banks C.A.S., Sorokina I., Florens L., Zybailov B., Washburn M.P., Brower C.S., Conaway R.C., Conaway J.W.
    J. Biol. Chem. 279:5846-5851(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE TRAP/SMCC MEDIATOR COMPLEX.
  8. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
    Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
    Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
  9. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
    Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
    Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CCNC; MED1; MED12; MED13; MED17; MED20 AND MED21, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
  10. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMED29_HUMAN
AccessioniPrimary (citable) accession number: Q9NX70
Secondary accession number(s): B4DNQ6
, M0R2E4, Q5XX09, Q9NTF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 1, 2000
Last modified: June 24, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.