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Protein

Mediator of RNA polymerase II transcription subunit 29

Gene

MED29

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-1989781. PPARA activates gene expression.
R-HSA-381340. Transcriptional regulation of white adipocyte differentiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 29
Alternative name(s):
Intersex-like protein
Mediator complex subunit 29
Gene namesi
Name:MED29
Synonyms:IXL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:23074. MED29.

Subcellular locationi

GO - Cellular componenti

  • mediator complex Source: MGI
  • nucleoplasm Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi55588.
OpenTargetsiENSG00000063322.
PharmGKBiPA162395655.

Polymorphism and mutation databases

DMDMi74734688.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002880582 – 200Mediator of RNA polymerase II transcription subunit 29Add BLAST199

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9NX70.
MaxQBiQ9NX70.
PaxDbiQ9NX70.
PeptideAtlasiQ9NX70.
PRIDEiQ9NX70.

PTM databases

iPTMnetiQ9NX70.
PhosphoSitePlusiQ9NX70.

Expressioni

Tissue specificityi

Widely expressed in embryo and adult.1 Publication

Gene expression databases

BgeeiENSG00000063322.
CleanExiHS_MED29.
ExpressionAtlasiQ9NX70. baseline and differential.
GenevisibleiQ9NX70. HS.

Organism-specific databases

HPAiHPA055956.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Associates with the MED18/MED20 heteromer.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Med11Q9D8C62EBI-394656,EBI-6260909From a different organism.
MED18Q9BUE05EBI-394656,EBI-394640
MED19A0JLT28EBI-394656,EBI-394430
MED20Q9H9445EBI-394656,EBI-394644
Med20Q9R0X02EBI-394656,EBI-398698From a different organism.
MED22Q155284EBI-394656,EBI-394687
MED28Q9H2043EBI-394656,EBI-514199
Med30Q9CQI92EBI-394656,EBI-309220From a different organism.
MED6O755863EBI-394656,EBI-394624
Med8Q9D7W52EBI-394656,EBI-7990252From a different organism.

Protein-protein interaction databases

BioGridi120734. 59 interactors.
IntActiQ9NX70. 90 interactors.
MINTiMINT-275786.
STRINGi9606.ENSP00000314343.

Structurei

3D structure databases

ProteinModelPortaliQ9NX70.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi29 – 84Gln-richAdd BLAST56

Sequence similaritiesi

Belongs to the Mediator complex subunit 29 family.Curated

Phylogenomic databases

eggNOGiENOG410IXGN. Eukaryota.
ENOG4111K6Y. LUCA.
GeneTreeiENSGT00390000007540.
HOVERGENiHBG060489.
InParanoidiQ9NX70.
KOiK15142.
PhylomeDBiQ9NX70.
TreeFamiTF326632.

Family and domain databases

InterProiIPR021018. Mediator_Med29_met.
[Graphical view]
PfamiPF11568. Med29. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NX70-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAASQQQASA ASSAAGVSGP SSAGGPGPQQ QPQPPAQLVG PAQSGLLQQQ
60 70 80 90 100
QQDFDPVQRY KMLIPQLKES LQTLMKVAAQ NLIQNTNIDN GQKSSDGPIQ
110 120 130 140 150
RFDKCLEEFY ALCDQLELCL RLAHECLSQS CDSAKHSPTL VPTATKPDAV
160 170 180 190 200
QPDSLPYPQY LAVIKAQISC AKDIHTALLD CANKVTGKTP APPAGPGGTL
Length:200
Mass (Da):21,073
Last modified:October 1, 2000 - v1
Checksum:iE0CDA1DDD89635CA
GO
Isoform 2 (identifier: Q9NX70-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     121-196: RLAHECLSQS...GKTPAPPAGP → PPSPTQCSLT...QWLVGGVQRE
     197-200: Missing.

Note: No experimental confirmation available.
Show »
Length:196
Mass (Da):21,362
Checksum:i5A3367EFACBD5356
GO

Sequence cautioni

The sequence AAU43732 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056133121 – 196RLAHE…PPAGP → PPSPTQCSLTASPTHSTWRS SKPRFPVPRTFTPPCWTVPT RSRARHPHHLLALGALCEVG DREWGRQWLVGGVQRE in isoform 2. 1 PublicationAdd BLAST76
Alternative sequenceiVSP_056134197 – 200Missing in isoform 2. 1 Publication4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY729650 mRNA. Translation: AAU43732.1. Different initiation.
AK000411 mRNA. Translation: BAA91147.1.
AK298014 mRNA. Translation: BAG60318.1.
AL137304 mRNA. Translation: CAB70687.2.
AC005239 Genomic DNA. No translation available.
AC011500 Genomic DNA. No translation available.
AC104106 Genomic DNA. No translation available.
BC019015 mRNA. Translation: AAH19015.1.
PIRiT46497.
RefSeqiNP_001304699.1. NM_001317770.1.
NP_060062.1. NM_017592.2.
UniGeneiHs.611541.

Genome annotation databases

EnsembliENST00000594368; ENSP00000472501; ENSG00000063322. [Q9NX70-2]
ENST00000599213; ENSP00000471802; ENSG00000063322. [Q9NX70-1]
GeneIDi55588.
KEGGihsa:55588.
UCSCiuc060ykk.1. human. [Q9NX70-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY729650 mRNA. Translation: AAU43732.1. Different initiation.
AK000411 mRNA. Translation: BAA91147.1.
AK298014 mRNA. Translation: BAG60318.1.
AL137304 mRNA. Translation: CAB70687.2.
AC005239 Genomic DNA. No translation available.
AC011500 Genomic DNA. No translation available.
AC104106 Genomic DNA. No translation available.
BC019015 mRNA. Translation: AAH19015.1.
PIRiT46497.
RefSeqiNP_001304699.1. NM_001317770.1.
NP_060062.1. NM_017592.2.
UniGeneiHs.611541.

3D structure databases

ProteinModelPortaliQ9NX70.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120734. 59 interactors.
IntActiQ9NX70. 90 interactors.
MINTiMINT-275786.
STRINGi9606.ENSP00000314343.

PTM databases

iPTMnetiQ9NX70.
PhosphoSitePlusiQ9NX70.

Polymorphism and mutation databases

DMDMi74734688.

Proteomic databases

EPDiQ9NX70.
MaxQBiQ9NX70.
PaxDbiQ9NX70.
PeptideAtlasiQ9NX70.
PRIDEiQ9NX70.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000594368; ENSP00000472501; ENSG00000063322. [Q9NX70-2]
ENST00000599213; ENSP00000471802; ENSG00000063322. [Q9NX70-1]
GeneIDi55588.
KEGGihsa:55588.
UCSCiuc060ykk.1. human. [Q9NX70-1]

Organism-specific databases

CTDi55588.
DisGeNETi55588.
GeneCardsiMED29.
H-InvDBHIX0015117.
HGNCiHGNC:23074. MED29.
HPAiHPA055956.
MIMi612914. gene.
neXtProtiNX_Q9NX70.
OpenTargetsiENSG00000063322.
PharmGKBiPA162395655.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IXGN. Eukaryota.
ENOG4111K6Y. LUCA.
GeneTreeiENSGT00390000007540.
HOVERGENiHBG060489.
InParanoidiQ9NX70.
KOiK15142.
PhylomeDBiQ9NX70.
TreeFamiTF326632.

Enzyme and pathway databases

ReactomeiR-HSA-1989781. PPARA activates gene expression.
R-HSA-381340. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

ChiTaRSiMED29. human.
GenomeRNAii55588.
PROiQ9NX70.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000063322.
CleanExiHS_MED29.
ExpressionAtlasiQ9NX70. baseline and differential.
GenevisibleiQ9NX70. HS.

Family and domain databases

InterProiIPR021018. Mediator_Med29_met.
[Graphical view]
PfamiPF11568. Med29. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMED29_HUMAN
AccessioniPrimary (citable) accession number: Q9NX70
Secondary accession number(s): B4DNQ6
, M0R2E4, Q5XX09, Q9NTF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.