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Q9NX70

- MED29_HUMAN

UniProt

Q9NX70 - MED29_HUMAN

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Protein

Mediator of RNA polymerase II transcription subunit 29

Gene

MED29

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.1 Publication

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_116145. PPARA activates gene expression.
REACT_27161. Transcriptional regulation of white adipocyte differentiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 29
Alternative name(s):
Intersex-like protein
Mediator complex subunit 29
Gene namesi
Name:MED29
Synonyms:IXL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:23074. MED29.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. mediator complex Source: MGI
  2. nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162395655.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 200200Mediator of RNA polymerase II transcription subunit 29PRO_0000288058Add
BLAST

Proteomic databases

MaxQBiQ9NX70.
PaxDbiQ9NX70.
PeptideAtlasiQ9NX70.
PRIDEiQ9NX70.

PTM databases

PhosphoSiteiQ9NX70.

Expressioni

Tissue specificityi

Widely expressed in embryo and adult.1 Publication

Gene expression databases

BgeeiQ9NX70.
CleanExiHS_MED29.
ExpressionAtlasiQ9NX70. baseline and differential.
GenevestigatoriQ9NX70.

Organism-specific databases

HPAiHPA055956.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Associates with the MED18/MED20 heteromer.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MED20Q9H9445EBI-394656,EBI-394644

Protein-protein interaction databases

BioGridi120734. 54 interactions.
IntActiQ9NX70. 83 interactions.
MINTiMINT-275786.
STRINGi9606.ENSP00000314343.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi29 – 8456Gln-richAdd
BLAST

Sequence similaritiesi

Belongs to the Mediator complex subunit 29 family.Curated

Phylogenomic databases

eggNOGiNOG48023.
GeneTreeiENSGT00390000007540.
HOVERGENiHBG060489.
InParanoidiQ9NX70.
KOiK15142.
PhylomeDBiQ9NX70.
TreeFamiTF326632.

Family and domain databases

InterProiIPR021018. Mediator_Med29_met.
[Graphical view]
PfamiPF11568. Med29. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9NX70-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAASQQQASA ASSAAGVSGP SSAGGPGPQQ QPQPPAQLVG PAQSGLLQQQ
60 70 80 90 100
QQDFDPVQRY KMLIPQLKES LQTLMKVAAQ NLIQNTNIDN GQKSSDGPIQ
110 120 130 140 150
RFDKCLEEFY ALCDQLELCL RLAHECLSQS CDSAKHSPTL VPTATKPDAV
160 170 180 190 200
QPDSLPYPQY LAVIKAQISC AKDIHTALLD CANKVTGKTP APPAGPGGTL
Length:200
Mass (Da):21,073
Last modified:October 1, 2000 - v1
Checksum:iE0CDA1DDD89635CA
GO
Isoform 2 (identifier: Q9NX70-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     121-196: RLAHECLSQS...GKTPAPPAGP → PPSPTQCSLT...QWLVGGVQRE
     197-200: Missing.

Note: No experimental confirmation available.

Show »
Length:196
Mass (Da):21,362
Checksum:i5A3367EFACBD5356
GO

Sequence cautioni

The sequence AAU43732.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei121 – 19676RLAHE…PPAGP → PPSPTQCSLTASPTHSTWRS SKPRFPVPRTFTPPCWTVPT RSRARHPHHLLALGALCEVG DREWGRQWLVGGVQRE in isoform 2. 1 PublicationVSP_056133Add
BLAST
Alternative sequencei197 – 2004Missing in isoform 2. 1 PublicationVSP_056134

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY729650 mRNA. Translation: AAU43732.1. Different initiation.
AK000411 mRNA. Translation: BAA91147.1.
AK298014 mRNA. Translation: BAG60318.1.
AL137304 mRNA. Translation: CAB70687.2.
AC005239 Genomic DNA. No translation available.
AC011500 Genomic DNA. No translation available.
AC104106 Genomic DNA. No translation available.
BC019015 mRNA. Translation: AAH19015.1.
PIRiT46497.
RefSeqiNP_060062.1. NM_017592.1.
UniGeneiHs.611541.

Genome annotation databases

EnsembliENST00000594368; ENSP00000472501; ENSG00000063322. [Q9NX70-2]
ENST00000599213; ENSP00000471802; ENSG00000063322. [Q9NX70-1]
GeneIDi55588.
KEGGihsa:55588.

Polymorphism databases

DMDMi74734688.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY729650 mRNA. Translation: AAU43732.1 . Different initiation.
AK000411 mRNA. Translation: BAA91147.1 .
AK298014 mRNA. Translation: BAG60318.1 .
AL137304 mRNA. Translation: CAB70687.2 .
AC005239 Genomic DNA. No translation available.
AC011500 Genomic DNA. No translation available.
AC104106 Genomic DNA. No translation available.
BC019015 mRNA. Translation: AAH19015.1 .
PIRi T46497.
RefSeqi NP_060062.1. NM_017592.1.
UniGenei Hs.611541.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 120734. 54 interactions.
IntActi Q9NX70. 83 interactions.
MINTi MINT-275786.
STRINGi 9606.ENSP00000314343.

PTM databases

PhosphoSitei Q9NX70.

Polymorphism databases

DMDMi 74734688.

Proteomic databases

MaxQBi Q9NX70.
PaxDbi Q9NX70.
PeptideAtlasi Q9NX70.
PRIDEi Q9NX70.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000594368 ; ENSP00000472501 ; ENSG00000063322 . [Q9NX70-2 ]
ENST00000599213 ; ENSP00000471802 ; ENSG00000063322 . [Q9NX70-1 ]
GeneIDi 55588.
KEGGi hsa:55588.

Organism-specific databases

CTDi 55588.
GeneCardsi GC19P039881.
H-InvDB HIX0015117.
HGNCi HGNC:23074. MED29.
HPAi HPA055956.
MIMi 612914. gene.
neXtProti NX_Q9NX70.
PharmGKBi PA162395655.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG48023.
GeneTreei ENSGT00390000007540.
HOVERGENi HBG060489.
InParanoidi Q9NX70.
KOi K15142.
PhylomeDBi Q9NX70.
TreeFami TF326632.

Enzyme and pathway databases

Reactomei REACT_116145. PPARA activates gene expression.
REACT_27161. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

ChiTaRSi MED29. human.
GenomeRNAii 55588.
NextBioi 35473673.
PROi Q9NX70.
SOURCEi Search...

Gene expression databases

Bgeei Q9NX70.
CleanExi HS_MED29.
ExpressionAtlasi Q9NX70. baseline and differential.
Genevestigatori Q9NX70.

Family and domain databases

InterProi IPR021018. Mediator_Med29_met.
[Graphical view ]
Pfami PF11568. Med29. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "IXL, a new subunit of the mammalian Mediator complex, functions as a transcriptional suppressor."
    Wang Y., Li Y., Zeng W., Zhu C., Xiao J., Yuan W., Wang Y., Cai Z., Zhou J., Liu M., Wu X.
    Biochem. Biophys. Res. Commun. 325:1330-1338(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Tissue: Embryonic heart.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Lung and Signet-ring cell carcinoma.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Testis.
  4. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  6. "A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex."
    Sato S., Tomomori-Sato C., Banks C.A.S., Parmely T.J., Sorokina I., Brower C.S., Conaway R.C., Conaway J.W.
    J. Biol. Chem. 278:49671-49674(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE TRAP/SMCC MEDIATOR COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH MED18 AND MED20.
  7. "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2."
    Tomomori-Sato C., Sato S., Parmely T.J., Banks C.A.S., Sorokina I., Florens L., Zybailov B., Washburn M.P., Brower C.S., Conaway R.C., Conaway J.W.
    J. Biol. Chem. 279:5846-5851(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE TRAP/SMCC MEDIATOR COMPLEX.
  8. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
    Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
    Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
  9. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
    Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
    Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CCNC; MED1; MED12; MED13; MED17; MED20 AND MED21, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.

Entry informationi

Entry nameiMED29_HUMAN
AccessioniPrimary (citable) accession number: Q9NX70
Secondary accession number(s): B4DNQ6
, M0R2E4, Q5XX09, Q9NTF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3