Q9NX70 (MED29_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mediator of RNA polymerase II transcription subunit 29

Alternative name(s):
Intersex-like protein
Mediator complex subunit 29

Gene names

Name:	MED29
Synonyms:	IXL

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Ref.1
Subunit structure	Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Associates with the MED18/MED20 heteromer. Ref.5 Ref.6 Ref.7 Ref.8
Subcellular location	Nucleus Ref.1.
Tissue specificity	Widely expressed in embryo and adult. Ref.1
Sequence similarities	Belongs to the Mediator complex subunit 29 family.
Sequence caution	The sequence AAU43732.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mediator complex Inferred from direct assay Ref.6. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MED20	Q9H944	5	EBI-394656,EBI-394644

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 200	200	Mediator of RNA polymerase II transcription subunit 29		PRO_0000288058
Regions
	Compositional bias	29 – 84	56	Gln-rich

Sequences

	Sequence		Length	Mass (Da)
	Q9NX70 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: E0CDA1DDD89635CA	FASTA	200	21,073
	10 20 30 40 50 60 MAASQQQASA ASSAAGVSGP SSAGGPGPQQ QPQPPAQLVG PAQSGLLQQQ QQDFDPVQRY 70 80 90 100 110 120 KMLIPQLKES LQTLMKVAAQ NLIQNTNIDN GQKSSDGPIQ RFDKCLEEFY ALCDQLELCL 130 140 150 160 170 180 RLAHECLSQS CDSAKHSPTL VPTATKPDAV QPDSLPYPQY LAVIKAQISC AKDIHTALLD 190 200 CANKVTGKTP APPAGPGGTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"IXL, a new subunit of the mammalian Mediator complex, functions as a transcriptional suppressor." Wang Y., Li Y., Zeng W., Zhu C., Xiao J., Yuan W., Wang Y., Cai Z., Zhou J., Liu M., Wu X. Biochem. Biophys. Res. Commun. 325:1330-1338(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION. Tissue: Embryonic heart.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Signet-ring cell carcinoma.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex." Sato S., Tomomori-Sato C., Banks C.A.S., Parmely T.J., Sorokina I., Brower C.S., Conaway R.C., Conaway J.W. J. Biol. Chem. 278:49671-49674(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE TRAP/SMCC MEDIATOR COMPLEX, MASS SPECTROMETRY, INTERACTION WITH MED18 AND MED20.
[6]	"A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2." Tomomori-Sato C., Sato S., Parmely T.J., Banks C.A.S., Sorokina I., Florens L., Zybailov B., Washburn M.P., Brower C.S., Conaway R.C., Conaway J.W. J. Biol. Chem. 279:5846-5851(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE TRAP/SMCC MEDIATOR COMPLEX.
[7]	"A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology." Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C. Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
[8]	"MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription." Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G. Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CCNC; MED1; MED12; MED13; MED17; MED20 AND MED21, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY729650 mRNA. Translation: AAU43732.1. Different initiation. AK000411 mRNA. Translation: BAA91147.1. AL137304 mRNA. Translation: CAB70687.2. BC019015 mRNA. Translation: AAH19015.1.
IPI	IPI00032780.
PIR	T46497.
RefSeq	NP_060062.1. NM_017592.1.
UniGene	Hs.611541.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NX70. 83 interactions.
MINT	MINT-275786.
STRING	9606.ENSP00000314343.
PTM databases
PhosphoSite	Q9NX70.
Polymorphism databases
DMDM	74734688.
Proteomic databases
PaxDb	Q9NX70.
PeptideAtlas	Q9NX70.
PRIDE	Q9NX70.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000315588; ENSP00000314343; ENSG00000063322.
GeneID	55588.
KEGG	hsa:55588.
Organism-specific databases
CTD	55588.
GeneCards	GC19P039881.
H-InvDB	HIX0015117.
HGNC	HGNC:23074. MED29.
MIM	612914. gene.
neXtProt	NX_Q9NX70.
PharmGKB	PA162395655.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG48023.
HOVERGEN	HBG060489.
InParanoid	Q9NX70.
KO	K15142.
OrthoDB	EOG4DJJXT.
Enzyme and pathway databases
Reactome	REACT_111045. Developmental Biology.
Gene expression databases
ArrayExpress	Q9NX70.
Bgee	Q9NX70.
CleanEx	HS_MED29.
Genevestigator	Q9NX70.
Family and domain databases
InterPro	IPR021018. Mediator_Med29_met. [Graphical view]
Pfam	PF11568. Med29. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	MED29. human.
GenomeRNAi	55588.
NextBio	60108.
SOURCE	Search...

Entry information

Entry name

MED29_HUMAN

Accession

Primary (citable) accession number: Q9NX70
Secondary accession number(s): Q5XX09, Q9NTF4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	October 1, 2000
Last modified:	April 3, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.