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Q9NX70

- MED29_HUMAN

UniProt

Q9NX70 - MED29_HUMAN

Protein

Mediator of RNA polymerase II transcription subunit 29

Gene

MED29

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    ReactomeiREACT_116145. PPARA activates gene expression.
    REACT_27161. Transcriptional regulation of white adipocyte differentiation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 29
    Alternative name(s):
    Intersex-like protein
    Mediator complex subunit 29
    Gene namesi
    Name:MED29
    Synonyms:IXL
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:23074. MED29.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. mediator complex Source: MGI
    2. nucleus Source: HPA

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162395655.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 200200Mediator of RNA polymerase II transcription subunit 29PRO_0000288058Add
    BLAST

    Proteomic databases

    MaxQBiQ9NX70.
    PaxDbiQ9NX70.
    PeptideAtlasiQ9NX70.
    PRIDEiQ9NX70.

    PTM databases

    PhosphoSiteiQ9NX70.

    Expressioni

    Tissue specificityi

    Widely expressed in embryo and adult.1 Publication

    Gene expression databases

    ArrayExpressiQ9NX70.
    BgeeiQ9NX70.
    CleanExiHS_MED29.
    GenevestigatoriQ9NX70.

    Organism-specific databases

    HPAiHPA055956.

    Interactioni

    Subunit structurei

    Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Associates with the MED18/MED20 heteromer.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MED20Q9H9445EBI-394656,EBI-394644

    Protein-protein interaction databases

    BioGridi120734. 52 interactions.
    IntActiQ9NX70. 83 interactions.
    MINTiMINT-275786.
    STRINGi9606.ENSP00000314343.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi29 – 8456Gln-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Mediator complex subunit 29 family.Curated

    Phylogenomic databases

    eggNOGiNOG48023.
    HOVERGENiHBG060489.
    InParanoidiQ9NX70.
    KOiK15142.
    PhylomeDBiQ9NX70.
    TreeFamiTF326632.

    Family and domain databases

    InterProiIPR021018. Mediator_Med29_met.
    [Graphical view]
    PfamiPF11568. Med29. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9NX70-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAASQQQASA ASSAAGVSGP SSAGGPGPQQ QPQPPAQLVG PAQSGLLQQQ    50
    QQDFDPVQRY KMLIPQLKES LQTLMKVAAQ NLIQNTNIDN GQKSSDGPIQ 100
    RFDKCLEEFY ALCDQLELCL RLAHECLSQS CDSAKHSPTL VPTATKPDAV 150
    QPDSLPYPQY LAVIKAQISC AKDIHTALLD CANKVTGKTP APPAGPGGTL 200
    Length:200
    Mass (Da):21,073
    Last modified:October 1, 2000 - v1
    Checksum:iE0CDA1DDD89635CA
    GO
    Isoform 2 (identifier: Q9NX70-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         121-196: RLAHECLSQS...GKTPAPPAGP → PPSPTQCSLT...QWLVGGVQRE
         197-200: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:196
    Mass (Da):21,362
    Checksum:i5A3367EFACBD5356
    GO

    Sequence cautioni

    The sequence AAU43732.1 differs from that shown. Reason: Erroneous initiation.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei121 – 19676RLAHE…PPAGP → PPSPTQCSLTASPTHSTWRS SKPRFPVPRTFTPPCWTVPT RSRARHPHHLLALGALCEVG DREWGRQWLVGGVQRE in isoform 2. 1 PublicationVSP_056133Add
    BLAST
    Alternative sequencei197 – 2004Missing in isoform 2. 1 PublicationVSP_056134

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY729650 mRNA. Translation: AAU43732.1. Different initiation.
    AK000411 mRNA. Translation: BAA91147.1.
    AK298014 mRNA. Translation: BAG60318.1.
    AL137304 mRNA. Translation: CAB70687.2.
    AC005239 Genomic DNA. No translation available.
    AC011500 Genomic DNA. No translation available.
    AC104106 Genomic DNA. No translation available.
    BC019015 mRNA. Translation: AAH19015.1.
    PIRiT46497.
    RefSeqiNP_060062.1. NM_017592.1.
    UniGeneiHs.611541.

    Genome annotation databases

    EnsembliENST00000594368; ENSP00000472501; ENSG00000063322.
    ENST00000599213; ENSP00000471802; ENSG00000063322.
    GeneIDi55588.
    KEGGihsa:55588.

    Polymorphism databases

    DMDMi74734688.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY729650 mRNA. Translation: AAU43732.1 . Different initiation.
    AK000411 mRNA. Translation: BAA91147.1 .
    AK298014 mRNA. Translation: BAG60318.1 .
    AL137304 mRNA. Translation: CAB70687.2 .
    AC005239 Genomic DNA. No translation available.
    AC011500 Genomic DNA. No translation available.
    AC104106 Genomic DNA. No translation available.
    BC019015 mRNA. Translation: AAH19015.1 .
    PIRi T46497.
    RefSeqi NP_060062.1. NM_017592.1.
    UniGenei Hs.611541.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 120734. 52 interactions.
    IntActi Q9NX70. 83 interactions.
    MINTi MINT-275786.
    STRINGi 9606.ENSP00000314343.

    PTM databases

    PhosphoSitei Q9NX70.

    Polymorphism databases

    DMDMi 74734688.

    Proteomic databases

    MaxQBi Q9NX70.
    PaxDbi Q9NX70.
    PeptideAtlasi Q9NX70.
    PRIDEi Q9NX70.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000594368 ; ENSP00000472501 ; ENSG00000063322 .
    ENST00000599213 ; ENSP00000471802 ; ENSG00000063322 .
    GeneIDi 55588.
    KEGGi hsa:55588.

    Organism-specific databases

    CTDi 55588.
    GeneCardsi GC19P039881.
    H-InvDB HIX0015117.
    HGNCi HGNC:23074. MED29.
    HPAi HPA055956.
    MIMi 612914. gene.
    neXtProti NX_Q9NX70.
    PharmGKBi PA162395655.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG48023.
    HOVERGENi HBG060489.
    InParanoidi Q9NX70.
    KOi K15142.
    PhylomeDBi Q9NX70.
    TreeFami TF326632.

    Enzyme and pathway databases

    Reactomei REACT_116145. PPARA activates gene expression.
    REACT_27161. Transcriptional regulation of white adipocyte differentiation.

    Miscellaneous databases

    ChiTaRSi MED29. human.
    GenomeRNAii 55588.
    NextBioi 60108.
    PROi Q9NX70.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9NX70.
    Bgeei Q9NX70.
    CleanExi HS_MED29.
    Genevestigatori Q9NX70.

    Family and domain databases

    InterProi IPR021018. Mediator_Med29_met.
    [Graphical view ]
    Pfami PF11568. Med29. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "IXL, a new subunit of the mammalian Mediator complex, functions as a transcriptional suppressor."
      Wang Y., Li Y., Zeng W., Zhu C., Xiao J., Yuan W., Wang Y., Cai Z., Zhou J., Liu M., Wu X.
      Biochem. Biophys. Res. Commun. 325:1330-1338(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
      Tissue: Embryonic heart.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Lung and Signet-ring cell carcinoma.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Testis.
    4. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    6. "A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex."
      Sato S., Tomomori-Sato C., Banks C.A.S., Parmely T.J., Sorokina I., Brower C.S., Conaway R.C., Conaway J.W.
      J. Biol. Chem. 278:49671-49674(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE TRAP/SMCC MEDIATOR COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH MED18 AND MED20.
    7. "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2."
      Tomomori-Sato C., Sato S., Parmely T.J., Banks C.A.S., Sorokina I., Florens L., Zybailov B., Washburn M.P., Brower C.S., Conaway R.C., Conaway J.W.
      J. Biol. Chem. 279:5846-5851(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE TRAP/SMCC MEDIATOR COMPLEX.
    8. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
      Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
      Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
    9. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
      Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
      Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CCNC; MED1; MED12; MED13; MED17; MED20 AND MED21, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.

    Entry informationi

    Entry nameiMED29_HUMAN
    AccessioniPrimary (citable) accession number: Q9NX70
    Secondary accession number(s): B4DNQ6
    , M0R2E4, Q5XX09, Q9NTF4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3