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Q9NX70 (MED29_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mediator of RNA polymerase II transcription subunit 29
Alternative name(s):
Intersex-like protein
Mediator complex subunit 29
Gene names
Name:MED29
Synonyms:IXL
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length200 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Ref.1

Subunit structure

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Associates with the MED18/MED20 heteromer. Ref.5 Ref.6 Ref.7 Ref.8

Subcellular location

Nucleus Ref.1.

Tissue specificity

Widely expressed in embryo and adult. Ref.1

Sequence similarities

Belongs to the Mediator complex subunit 29 family.

Sequence caution

The sequence AAU43732.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Molecular functionActivator
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processregulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentmediator complex

Inferred from direct assay Ref.6. Source: MGI

nucleus

Inferred from direct assay. Source: HPA

   Molecular_functionprotein binding

Inferred from physical interaction Ref.5Ref.7. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MED20Q9H9445EBI-394656,EBI-394644

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 200200Mediator of RNA polymerase II transcription subunit 29
PRO_0000288058

Regions

Compositional bias29 – 8456Gln-rich

Sequences

Sequence LengthMass (Da)Tools
Q9NX70 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: E0CDA1DDD89635CA

FASTA20021,073
        10         20         30         40         50         60 
MAASQQQASA ASSAAGVSGP SSAGGPGPQQ QPQPPAQLVG PAQSGLLQQQ QQDFDPVQRY 

        70         80         90        100        110        120 
KMLIPQLKES LQTLMKVAAQ NLIQNTNIDN GQKSSDGPIQ RFDKCLEEFY ALCDQLELCL 

       130        140        150        160        170        180 
RLAHECLSQS CDSAKHSPTL VPTATKPDAV QPDSLPYPQY LAVIKAQISC AKDIHTALLD 

       190        200 
CANKVTGKTP APPAGPGGTL 

« Hide

References

« Hide 'large scale' references
[1]"IXL, a new subunit of the mammalian Mediator complex, functions as a transcriptional suppressor."
Wang Y., Li Y., Zeng W., Zhu C., Xiao J., Yuan W., Wang Y., Cai Z., Zhou J., Liu M., Wu X.
Biochem. Biophys. Res. Commun. 325:1330-1338(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
Tissue: Embryonic heart.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Signet-ring cell carcinoma.
[3]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[5]"A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex."
Sato S., Tomomori-Sato C., Banks C.A.S., Parmely T.J., Sorokina I., Brower C.S., Conaway R.C., Conaway J.W.
J. Biol. Chem. 278:49671-49674(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TRAP/SMCC MEDIATOR COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH MED18 AND MED20.
[6]"A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2."
Tomomori-Sato C., Sato S., Parmely T.J., Banks C.A.S., Sorokina I., Florens L., Zybailov B., Washburn M.P., Brower C.S., Conaway R.C., Conaway J.W.
J. Biol. Chem. 279:5846-5851(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TRAP/SMCC MEDIATOR COMPLEX.
[7]"A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
[8]"MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CCNC; MED1; MED12; MED13; MED17; MED20 AND MED21, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY729650 mRNA. Translation: AAU43732.1. Different initiation.
AK000411 mRNA. Translation: BAA91147.1.
AL137304 mRNA. Translation: CAB70687.2.
BC019015 mRNA. Translation: AAH19015.1.
PIRT46497.
RefSeqNP_060062.1. NM_017592.1.
UniGeneHs.611541.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120734. 52 interactions.
IntActQ9NX70. 83 interactions.
MINTMINT-275786.
STRING9606.ENSP00000314343.

PTM databases

PhosphoSiteQ9NX70.

Polymorphism databases

DMDM74734688.

Proteomic databases

MaxQBQ9NX70.
PaxDbQ9NX70.
PeptideAtlasQ9NX70.
PRIDEQ9NX70.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000599213; ENSP00000471802; ENSG00000063322.
GeneID55588.
KEGGhsa:55588.

Organism-specific databases

CTD55588.
GeneCardsGC19P039881.
H-InvDBHIX0015117.
HGNCHGNC:23074. MED29.
HPAHPA055956.
MIM612914. gene.
neXtProtNX_Q9NX70.
PharmGKBPA162395655.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG48023.
HOVERGENHBG060489.
InParanoidQ9NX70.
KOK15142.
PhylomeDBQ9NX70.
TreeFamTF326632.

Enzyme and pathway databases

ReactomeREACT_111045. Developmental Biology.

Gene expression databases

ArrayExpressQ9NX70.
BgeeQ9NX70.
CleanExHS_MED29.
GenevestigatorQ9NX70.

Family and domain databases

InterProIPR021018. Mediator_Med29_met.
[Graphical view]
PfamPF11568. Med29. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMED29. human.
GenomeRNAi55588.
NextBio60108.
PROQ9NX70.
SOURCESearch...

Entry information

Entry nameMED29_HUMAN
AccessionPrimary (citable) accession number: Q9NX70
Secondary accession number(s): Q5XX09, Q9NTF4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM