ID TSAP1_HUMAN Reviewed; 287 AA. AC Q9NX07; Q86SU7; DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-2000, sequence version 1. DT 27-MAR-2024, entry version 182. DE RecName: Full=tRNA selenocysteine 1-associated protein 1; DE AltName: Full=SECp43; DE AltName: Full=tRNA selenocysteine-associated protein 1; GN Name=TRNAU1AP; Synonyms=SECP43, TRSPAP1; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; OC Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). RX PubMed=14702039; DOI=10.1038/ng1285; RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., RA Isogai T., Sugano S.; RT "Complete sequencing and characterization of 21,243 full-length human RT cDNAs."; RL Nat. Genet. 36:40-45(2004). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=16710414; DOI=10.1038/nature04727; RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K., RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.; RT "The DNA sequence and biological annotation of human chromosome 1."; RL Nature 441:315-321(2006). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., RA Hunkapiller M.W., Myers E.W., Venter J.C.; RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). RC TISSUE=Brain, and Colon; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [5] RP FUNCTION, AND IDENTIFICATION IN A COMPLEX WITH EEFSEC; SECISBP2 AND RP TRNA(SEC). RX PubMed=16508009; DOI=10.1128/mcb.26.6.2337-2346.2006; RA Small-Howard A., Morozova N., Stoytcheva Z., Forry E.P., Mansell J.B., RA Harney J.W., Carlson B.A., Xu X.M., Hatfield D.L., Berry M.J.; RT "Supramolecular complexes mediate selenocysteine incorporation in vivo."; RL Mol. Cell. Biol. 26:2337-2346(2006). RN [6] RP STRUCTURE BY NMR OF 1-184. RG RIKEN structural genomics initiative (RSGI); RT "Solution structure of the RRM domain and of the C-terminal RNA recognition RT motif of tRNA selenocysteine associated protein."; RL Submitted (SEP-2006) to the PDB data bank. CC -!- FUNCTION: Involved in the early steps of selenocysteine biosynthesis CC and tRNA(Sec) charging to the later steps resulting in the CC cotranslational incorporation of selenocysteine into selenoproteins. CC Stabilizes the SECISBP2, EEFSEC and tRNA(Sec) complex. May be involved CC in the methylation of tRNA(Sec). Enhances efficiency of selenoproteins CC synthesis (By similarity). {ECO:0000250, ECO:0000269|PubMed:16508009}. CC -!- SUBUNIT: Component of the tRNA(Sec) complex composed at least of CC EEFSEC, SECISBP2, SEPHS1, SEPSECS, TRNAU1AP and tRNA(Sec). Found in a CC complex with tRNA(Sec). Interacts with SEPSECS. Associates with mRNP CC and/or polysomes (By similarity). Found in a complex with EEFSEC, CC SECISBP2, TRNAU1AP and tRNA(Sec). {ECO:0000250, CC ECO:0000269|PubMed:16508009}. CC -!- INTERACTION: CC Q9NX07; P27797: CALR; NbExp=3; IntAct=EBI-12581310, EBI-1049597; CC Q9NX07; P36957: DLST; NbExp=3; IntAct=EBI-12581310, EBI-351007; CC Q9NX07; Q92876: KLK6; NbExp=3; IntAct=EBI-12581310, EBI-2432309; CC Q9NX07; Q8TDX7: NEK7; NbExp=3; IntAct=EBI-12581310, EBI-1055945; CC Q9NX07-2; P50570-2: DNM2; NbExp=3; IntAct=EBI-21894090, EBI-10968534; CC Q9NX07-2; Q16637: SMN2; NbExp=3; IntAct=EBI-21894090, EBI-395421; CC Q9NX07-2; O14656-2: TOR1A; NbExp=3; IntAct=EBI-21894090, EBI-25847109; CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}. CC Note=Abundant in the nucleus. {ECO:0000250}. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=2; CC Name=1; CC IsoId=Q9NX07-1; Sequence=Displayed; CC Name=2; CC IsoId=Q9NX07-2; Sequence=VSP_028130; CC -!- SIMILARITY: Belongs to the RRM TRSPAP family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AK000510; BAA91217.1; -; mRNA. DR EMBL; AL513497; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; CH471059; EAX07689.1; -; Genomic_DNA. DR EMBL; BC000680; AAH00680.1; -; mRNA. DR EMBL; BC039879; AAH39879.1; -; mRNA. DR CCDS; CCDS324.1; -. [Q9NX07-1] DR RefSeq; NP_060316.1; NM_017846.4. [Q9NX07-1] DR RefSeq; XP_016857031.1; XM_017001542.1. DR PDB; 2DHG; NMR; -; A=94-184. DR PDB; 2DIV; NMR; -; A=1-86. DR PDBsum; 2DHG; -. DR PDBsum; 2DIV; -. DR AlphaFoldDB; Q9NX07; -. DR SMR; Q9NX07; -. DR BioGRID; 120290; 44. DR IntAct; Q9NX07; 12. DR MINT; Q9NX07; -. DR STRING; 9606.ENSP00000362936; -. DR iPTMnet; Q9NX07; -. DR PhosphoSitePlus; Q9NX07; -. DR BioMuta; TRNAU1AP; -. DR DMDM; 74761781; -. DR EPD; Q9NX07; -. DR jPOST; Q9NX07; -. DR MassIVE; Q9NX07; -. DR MaxQB; Q9NX07; -. DR PaxDb; 9606-ENSP00000362936; -. DR PeptideAtlas; Q9NX07; -. DR ProteomicsDB; 83017; -. [Q9NX07-1] DR ProteomicsDB; 83018; -. [Q9NX07-2] DR Pumba; Q9NX07; -. DR Antibodypedia; 30961; 164 antibodies from 23 providers. DR DNASU; 54952; -. DR Ensembl; ENST00000373830.4; ENSP00000362936.3; ENSG00000180098.9. [Q9NX07-1] DR GeneID; 54952; -. DR KEGG; hsa:54952; -. DR MANE-Select; ENST00000373830.4; ENSP00000362936.3; NM_017846.5; NP_060316.1. DR UCSC; uc001bqi.4; human. [Q9NX07-1] DR AGR; HGNC:30813; -. DR CTD; 54952; -. DR GeneCards; TRNAU1AP; -. DR HGNC; HGNC:30813; TRNAU1AP. DR HPA; ENSG00000180098; Low tissue specificity. DR MIM; 619597; gene. DR neXtProt; NX_Q9NX07; -. DR OpenTargets; ENSG00000180098; -. DR PharmGKB; PA164727240; -. DR VEuPathDB; HostDB:ENSG00000180098; -. DR eggNOG; KOG0118; Eukaryota. DR GeneTree; ENSGT00940000156139; -. DR HOGENOM; CLU_016304_3_0_1; -. DR InParanoid; Q9NX07; -. DR OMA; SSKWIPC; -. DR OrthoDB; 129910at2759; -. DR PhylomeDB; Q9NX07; -. DR TreeFam; TF313275; -. DR PathwayCommons; Q9NX07; -. DR SignaLink; Q9NX07; -. DR BioGRID-ORCS; 54952; 235 hits in 1169 CRISPR screens. DR ChiTaRS; TRNAU1AP; human. DR EvolutionaryTrace; Q9NX07; -. DR GenomeRNAi; 54952; -. DR Pharos; Q9NX07; Tbio. DR PRO; PR:Q9NX07; -. DR Proteomes; UP000005640; Chromosome 1. DR RNAct; Q9NX07; Protein. DR Bgee; ENSG00000180098; Expressed in granulocyte and 167 other cell types or tissues. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0005634; C:nucleus; ISS:HGNC-UCL. DR GO; GO:0003723; F:RNA binding; HDA:UniProtKB. DR GO; GO:0000049; F:tRNA binding; IBA:GO_Central. DR GO; GO:0001514; P:selenocysteine incorporation; ISS:HGNC-UCL. DR CDD; cd12610; RRM1_SECp43; 1. DR CDD; cd12612; RRM2_SECp43; 1. DR Gene3D; 3.30.70.330; -; 2. DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf. DR InterPro; IPR035979; RBD_domain_sf. DR InterPro; IPR000504; RRM_dom. DR InterPro; IPR034510; SECp43_RRM2. DR InterPro; IPR040434; TSAP1. DR InterPro; IPR041085; TSAP1_C. DR PANTHER; PTHR37457:SF2; TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1; 1. DR PANTHER; PTHR37457; TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED; 1. DR Pfam; PF00076; RRM_1; 2. DR Pfam; PF17654; Trnau1ap; 1. DR SMART; SM00360; RRM; 2. DR SUPFAM; SSF54928; RNA-binding domain, RBD; 1. DR PROSITE; PS50102; RRM; 2. DR Genevisible; Q9NX07; HS. PE 1: Evidence at protein level; KW 3D-structure; Alternative splicing; Cytoplasm; Nucleus; KW Protein biosynthesis; Reference proteome; Repeat; RNA-binding. FT CHAIN 1..287 FT /note="tRNA selenocysteine 1-associated protein 1" FT /id="PRO_0000304917" FT DOMAIN 3..86 FT /note="RRM 1" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176" FT DOMAIN 96..175 FT /note="RRM 2" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176" FT VAR_SEQ 1..110 FT /note="Missing (in isoform 2)" FT /evidence="ECO:0000303|PubMed:15489334" FT /id="VSP_028130" FT STRAND 1..7 FT /evidence="ECO:0007829|PDB:2DIV" FT HELIX 16..25 FT /evidence="ECO:0007829|PDB:2DIV" FT STRAND 32..37 FT /evidence="ECO:0007829|PDB:2DIV" FT STRAND 39..41 FT /evidence="ECO:0007829|PDB:2DIV" FT STRAND 44..51 FT /evidence="ECO:0007829|PDB:2DIV" FT HELIX 55..63 FT /evidence="ECO:0007829|PDB:2DIV" FT TURN 64..67 FT /evidence="ECO:0007829|PDB:2DIV" FT STRAND 68..72 FT /evidence="ECO:0007829|PDB:2DIV" FT STRAND 81..83 FT /evidence="ECO:0007829|PDB:2DIV" FT STRAND 98..101 FT /evidence="ECO:0007829|PDB:2DHG" FT HELIX 109..119 FT /evidence="ECO:0007829|PDB:2DHG" FT STRAND 123..130 FT /evidence="ECO:0007829|PDB:2DHG" FT STRAND 136..145 FT /evidence="ECO:0007829|PDB:2DHG" FT HELIX 147..156 FT /evidence="ECO:0007829|PDB:2DHG" FT TURN 157..159 FT /evidence="ECO:0007829|PDB:2DHG" FT STRAND 162..166 FT /evidence="ECO:0007829|PDB:2DHG" SQ SEQUENCE 287 AA; 32499 MW; 4F85AA839FF256F5 CRC64; MAASLWMGDL EPYMDENFIS RAFATMGETV MSVKIIRNRL TGIPAGYCFV EFADLATAEK CLHKINGKPL PGATPAKRFK LNYATYGKQP DNSPEYSLFV GDLTPDVDDG MLYEFFVKVY PSCRGGKVVL DQTGVSKGYG FVKFTDELEQ KRALTECQGA VGLGSKPVRL SVAIPKASRV KPVEYSQMYS YSYNQYYQQY QNYYAQWGYD QNTGSYSYSY PQYGYTQSTM QTYEEVGDDA LEDPMPQLDV TEANKEFMEQ SEELYDALMD CHWQPLDTVS SEIPAMM //