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Protein

tRNA selenocysteine 1-associated protein 1

Gene

TRNAU1AP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the early steps of selenocysteine biosynthesis and tRNA(Sec) charging to the later steps resulting in the cotranslational incorporation of selenocysteine into selenoproteins. Stabilizes the SECISBP2, EEFSEC and tRNA(Sec) complex. May be involved in the methylation of tRNA(Sec). Enhances efficiency of selenoproteins synthesis (By similarity).By similarity

GO - Molecular functioni

  1. nucleotide binding Source: InterPro
  2. poly(A) RNA binding Source: UniProtKB

GO - Biological processi

  1. selenocysteine incorporation Source: HGNC
Complete GO annotation...

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA selenocysteine 1-associated protein 1
Alternative name(s):
SECp43
tRNA selenocysteine-associated protein 1
Gene namesi
Name:TRNAU1AP
Synonyms:SECP43, TRSPAP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 1

Organism-specific databases

HGNCiHGNC:30813. TRNAU1AP.

Subcellular locationi

Nucleus By similarity. Cytoplasm By similarity
Note: Abundant in the nucleus.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA164727240.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 287287tRNA selenocysteine 1-associated protein 1PRO_0000304917Add
BLAST

Proteomic databases

MaxQBiQ9NX07.
PaxDbiQ9NX07.
PRIDEiQ9NX07.

PTM databases

PhosphoSiteiQ9NX07.

Expressioni

Gene expression databases

BgeeiQ9NX07.
CleanExiHS_TRNAU1AP.
GenevestigatoriQ9NX07.

Organism-specific databases

HPAiHPA032069.

Interactioni

Subunit structurei

Component of the tRNA(Sec) complex composed at least of EEFSEC, SECISBP2, SEPHS1, SEPSECS, TRNAU1AP and tRNA(Sec). Found in a complex with tRNA(Sec). Interacts with SEPSECS. Associates with mRNP and/or polysomes (By similarity). Found in a complex with EEFSEC, SECISBP2, TRNAU1AP and tRNA(Sec).By similarity1 Publication

Protein-protein interaction databases

BioGridi120290. 5 interactions.
STRINGi9606.ENSP00000362936.

Structurei

Secondary structure

287
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1 – 77Combined sources
Helixi16 – 2510Combined sources
Beta strandi32 – 376Combined sources
Beta strandi39 – 413Combined sources
Beta strandi44 – 518Combined sources
Helixi55 – 639Combined sources
Turni64 – 674Combined sources
Beta strandi68 – 725Combined sources
Beta strandi81 – 833Combined sources
Beta strandi98 – 1014Combined sources
Helixi109 – 11911Combined sources
Beta strandi123 – 1308Combined sources
Beta strandi136 – 14510Combined sources
Helixi147 – 15610Combined sources
Turni157 – 1593Combined sources
Beta strandi162 – 1665Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DHGNMR-A94-184[»]
2DIVNMR-A1-86[»]
ProteinModelPortaliQ9NX07.
SMRiQ9NX07. Positions 1-184.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NX07.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 8684RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini96 – 17580RRM 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi185 – 22541Tyr-richAdd
BLAST

Sequence similaritiesi

Belongs to the RRM TRSPAP family.Curated
Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0724.
GeneTreeiENSGT00510000047100.
HOGENOMiHOG000234338.
HOVERGENiHBG056959.
InParanoidiQ9NX07.
OMAiDENFVSR.
OrthoDBiEOG76473F.
PhylomeDBiQ9NX07.
TreeFamiTF313275.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9NX07-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAASLWMGDL EPYMDENFIS RAFATMGETV MSVKIIRNRL TGIPAGYCFV
60 70 80 90 100
EFADLATAEK CLHKINGKPL PGATPAKRFK LNYATYGKQP DNSPEYSLFV
110 120 130 140 150
GDLTPDVDDG MLYEFFVKVY PSCRGGKVVL DQTGVSKGYG FVKFTDELEQ
160 170 180 190 200
KRALTECQGA VGLGSKPVRL SVAIPKASRV KPVEYSQMYS YSYNQYYQQY
210 220 230 240 250
QNYYAQWGYD QNTGSYSYSY PQYGYTQSTM QTYEEVGDDA LEDPMPQLDV
260 270 280
TEANKEFMEQ SEELYDALMD CHWQPLDTVS SEIPAMM
Length:287
Mass (Da):32,499
Last modified:October 1, 2000 - v1
Checksum:i4F85AA839FF256F5
GO
Isoform 2 (identifier: Q9NX07-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-110: Missing.

Show »
Length:177
Mass (Da):20,403
Checksum:i5E08BFD70AA68A7C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 110110Missing in isoform 2. 1 PublicationVSP_028130Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000510 mRNA. Translation: BAA91217.1.
AL513497 Genomic DNA. Translation: CAI22288.1.
CH471059 Genomic DNA. Translation: EAX07689.1.
BC000680 mRNA. Translation: AAH00680.1.
BC039879 mRNA. Translation: AAH39879.1.
CCDSiCCDS324.1. [Q9NX07-1]
RefSeqiNP_060316.1. NM_017846.4. [Q9NX07-1]
UniGeneiHs.533626.

Genome annotation databases

EnsembliENST00000373830; ENSP00000362936; ENSG00000180098. [Q9NX07-1]
GeneIDi54952.
KEGGihsa:54952.
UCSCiuc001bqi.3. human. [Q9NX07-1]

Polymorphism databases

DMDMi74761781.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000510 mRNA. Translation: BAA91217.1.
AL513497 Genomic DNA. Translation: CAI22288.1.
CH471059 Genomic DNA. Translation: EAX07689.1.
BC000680 mRNA. Translation: AAH00680.1.
BC039879 mRNA. Translation: AAH39879.1.
CCDSiCCDS324.1. [Q9NX07-1]
RefSeqiNP_060316.1. NM_017846.4. [Q9NX07-1]
UniGeneiHs.533626.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DHGNMR-A94-184[»]
2DIVNMR-A1-86[»]
ProteinModelPortaliQ9NX07.
SMRiQ9NX07. Positions 1-184.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120290. 5 interactions.
STRINGi9606.ENSP00000362936.

PTM databases

PhosphoSiteiQ9NX07.

Polymorphism databases

DMDMi74761781.

Proteomic databases

MaxQBiQ9NX07.
PaxDbiQ9NX07.
PRIDEiQ9NX07.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000373830; ENSP00000362936; ENSG00000180098. [Q9NX07-1]
GeneIDi54952.
KEGGihsa:54952.
UCSCiuc001bqi.3. human. [Q9NX07-1]

Organism-specific databases

CTDi54952.
GeneCardsiGC01P028879.
H-InvDBHIX0000337.
HGNCiHGNC:30813. TRNAU1AP.
HPAiHPA032069.
neXtProtiNX_Q9NX07.
PharmGKBiPA164727240.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0724.
GeneTreeiENSGT00510000047100.
HOGENOMiHOG000234338.
HOVERGENiHBG056959.
InParanoidiQ9NX07.
OMAiDENFVSR.
OrthoDBiEOG76473F.
PhylomeDBiQ9NX07.
TreeFamiTF313275.

Miscellaneous databases

ChiTaRSiTRNAU1AP. human.
EvolutionaryTraceiQ9NX07.
GenomeRNAii54952.
NextBioi58127.
PROiQ9NX07.

Gene expression databases

BgeeiQ9NX07.
CleanExiHS_TRNAU1AP.
GenevestigatoriQ9NX07.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  2. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain and Colon.
  5. Cited for: FUNCTION, IDENTIFICATION IN A COMPLEX WITH EEFSEC; SECISBP2 AND TRNA(SEC).
  6. "Solution structure of the RRM domain and of the C-terminal RNA recognition motif of tRNA selenocysteine associated protein."
    RIKEN structural genomics initiative (RSGI)
    Submitted (SEP-2006) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 1-184.

Entry informationi

Entry nameiTSAP1_HUMAN
AccessioniPrimary (citable) accession number: Q9NX07
Secondary accession number(s): Q86SU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 1, 2000
Last modified: January 7, 2015
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.