Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable tRNA(His) guanylyltransferase

Gene

THG1L

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. This step is essential for proper recognition of the tRNA and for the fidelity of protein synthesis.1 Publication

Catalytic activityi

p-tRNA(His) + ATP + GTP = pppG-P-tRNA(His) + AMP + diphosphate.

Cofactori

Mg2+1 PublicationNote: Binds 2 magnesium ions per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi58Magnesium 1; catalytic1 Publication1
Metal bindingi58Magnesium 2; catalytic1 Publication1
Metal bindingi59Magnesium 1; via carbonyl oxygen; catalytic1 Publication1
Metal bindingi105Magnesium 1; catalytic1 Publication1
Metal bindingi105Magnesium 2; catalytic1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi58 – 63GTP1 Publication6
Nucleotide bindingi104 – 105GTP1 Publication2

GO - Molecular functioni

  • ATP binding Source: BHF-UCL
  • GTP binding Source: BHF-UCL
  • identical protein binding Source: IntAct
  • magnesium ion binding Source: BHF-UCL
  • nucleotidyltransferase activity Source: Reactome
  • tRNA binding Source: BHF-UCL
  • tRNA guanylyltransferase activity Source: UniProtKB

GO - Biological processi

  • protein homotetramerization Source: BHF-UCL
  • tRNA modification Source: UniProtKB
  • tRNA processing Source: UniProtKB

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processtRNA processing
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.7.79 2681
ReactomeiR-HSA-6782315 tRNA modification in the nucleus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA(His) guanylyltransferase (EC:2.7.7.79)
Alternative name(s):
Interphase cytoplasmic foci protein 45
tRNA-histidine guanylyltransferase
Gene namesi
Name:THG1L
Synonyms:ICF45
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000113272.13
HGNCiHGNC:26053 THG1L
neXtProtiNX_Q9NWX6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi58D → A: Reduces activity by 99.5%. 1 Publication1
Mutagenesisi63H → A: Slightly reduced enzyme activity. 1 Publication1
Mutagenesisi104S → A: Slightly reduced enzyme activity. 1 Publication1
Mutagenesisi105D → A: Loss of enzyme activity. 1 Publication1
Mutagenesisi106E → A: Reduces activity by 95%. 1 Publication1
Mutagenesisi127T → A: Abolishes oligomerization. Loss of enzyme activity. 1 Publication1
Mutagenesisi181H → A: Loss of enzyme activity. 1 Publication1
Mutagenesisi216K → A: Reduces activity by 98.5%. 1 Publication1
Mutagenesisi227N → A: Loss of enzyme activity. 1 Publication1

Organism-specific databases

DisGeNETi54974
OpenTargetsiENSG00000113272
PharmGKBiPA162405691

Polymorphism and mutation databases

BioMutaiTHG1L
DMDMi146325755

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002849841 – 298Probable tRNA(His) guanylyltransferaseAdd BLAST298

Proteomic databases

EPDiQ9NWX6
MaxQBiQ9NWX6
PaxDbiQ9NWX6
PeptideAtlasiQ9NWX6
PRIDEiQ9NWX6

PTM databases

iPTMnetiQ9NWX6
PhosphoSitePlusiQ9NWX6

Expressioni

Tissue specificityi

Expressed in many tissues.1 Publication

Gene expression databases

BgeeiENSG00000113272
CleanExiHS_THG1L
ExpressionAtlasiQ9NWX6 baseline and differential
GenevisibleiQ9NWX6 HS

Organism-specific databases

HPAiHPA035877
HPA035878

Interactioni

Subunit structurei

Homotetramer.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi120311, 29 interactors
DIPiDIP-59479N
IntActiQ9NWX6, 8 interactors
MINTiQ9NWX6
STRINGi9606.ENSP00000231198

Structurei

Secondary structure

1298
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi36 – 41Combined sources6
Beta strandi51 – 59Combined sources9
Helixi62 – 68Combined sources7
Helixi77 – 93Combined sources17
Beta strandi94 – 103Combined sources10
Beta strandi106 – 111Combined sources6
Turni117 – 120Combined sources4
Helixi122 – 139Combined sources18
Helixi141 – 144Combined sources4
Beta strandi146 – 148Combined sources3
Beta strandi156 – 165Combined sources10
Helixi166 – 195Combined sources30
Helixi201 – 208Combined sources8
Helixi213 – 224Combined sources12
Helixi228 – 230Combined sources3
Helixi233 – 237Combined sources5
Beta strandi239 – 243Combined sources5
Beta strandi274 – 277Combined sources4
Beta strandi281 – 283Combined sources3
Helixi284 – 289Combined sources6
Helixi292 – 295Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3OTBX-ray2.95A/B30-298[»]
3OTCX-ray3.01A/B30-298[»]
3OTDX-ray2.28A/B30-298[»]
3OTEX-ray2.56A/B30-298[»]
ProteinModelPortaliQ9NWX6
SMRiQ9NWX6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NWX6

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2721 Eukaryota
COG4021 LUCA
GeneTreeiENSGT00390000011705
HOGENOMiHOG000204890
HOVERGENiHBG054289
InParanoidiQ9NWX6
KOiK10761
OMAiNTAFWKL
OrthoDBiEOG091G0GYS
PhylomeDBiQ9NWX6
TreeFamiTF325119

Family and domain databases

Gene3Di3.30.70.3000, 1 hit
InterProiView protein in InterPro
IPR025845 Thg1_C_dom
IPR024956 tRNAHis_GuaTrfase_cat
IPR007537 tRNAHis_GuaTrfase_Thg1
IPR038469 tRNAHis_GuaTrfase_Thg1_sf
PANTHERiPTHR12729 PTHR12729, 1 hit
PfamiView protein in Pfam
PF04446 Thg1, 1 hit
PF14413 Thg1C, 1 hit
PIRSFiPIRSF028980 tRNAHis_guanylyltransferase, 1 hit

Sequencei

Sequence statusi: Complete.

Q9NWX6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWGACKVKVH DSLATISITL RRYLRLGATM AKSKFEYVRD FEADDTCLAH
60 70 80 90 100
CWVVVRLDGR NFHRFAEKHN FAKPNDSRAL QLMTKCAQTV MEELEDIVIA
110 120 130 140 150
YGQSDEYSFV FKRKTNWFKR RASKFMTHVA SQFASSYVFY WRDYFEDQPL
160 170 180 190 200
LYPPGFDGRV VVYPSNQTLK DYLSWRQADC HINNLYNTVF WALIQQSGLT
210 220 230 240 250
PVQAQGRLQG TLAADKNEIL FSEFNINYNN ELPMYRKGTV LIWQKVDEVM
260 270 280 290
TKEIKLPTEM EGKKMAVTRT RTKPVPLHCD IIGDAFWKEH PEILDEDS
Length:298
Mass (Da):34,831
Last modified:May 1, 2007 - v2
Checksum:iA6C941B75611C448
GO

Sequence cautioni

The sequence AAH01523 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH01852 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti57L → Q in BAD96839 (Ref. 5) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_031871232L → P4 PublicationsCorresponds to variant dbSNP:rs2270812Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY463216 mRNA Translation: AAS21134.1
AL136669 mRNA Translation: CAB66604.1
AK000553 mRNA Translation: BAA91249.1
AK021663 mRNA Translation: BAB13870.1
CR533503 mRNA Translation: CAG38534.1
AK223119 mRNA Translation: BAD96839.1
CH471062 Genomic DNA Translation: EAW61590.1
CH471062 Genomic DNA Translation: EAW61591.1
BC001523 mRNA Translation: AAH01523.2 Different initiation.
BC001852 mRNA Translation: AAH01852.2 Different initiation.
BC023521 mRNA Translation: AAH23521.1
CCDSiCCDS4341.1
RefSeqiNP_001304753.1, NM_001317824.1
NP_001304754.1, NM_001317825.1
NP_001304755.1, NM_001317826.1
NP_060342.2, NM_017872.4
UniGeneiHs.353090
Hs.734786

Genome annotation databases

EnsembliENST00000231198; ENSP00000231198; ENSG00000113272
GeneIDi54974
KEGGihsa:54974
UCSCiuc003lxd.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTHG1_HUMAN
AccessioniPrimary (citable) accession number: Q9NWX6
Secondary accession number(s): D3DQJ5
, Q53G12, Q7L5R3, Q9H0S2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: March 28, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health