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Protein

Probable tRNA(His) guanylyltransferase

Gene

THG1L

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. This step is essential for proper recognition of the tRNA and for the fidelity of protein synthesis.1 Publication

Catalytic activityi

p-tRNA(His) + ATP + GTP = pppG-P-tRNA(His) + AMP + diphosphate.

Cofactori

Mg2+1 PublicationNote: Binds 2 magnesium ions per subunit.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi58 – 581Magnesium 1; catalytic1 Publication
Metal bindingi58 – 581Magnesium 2; catalytic1 Publication
Metal bindingi59 – 591Magnesium 1; via carbonyl oxygen; catalytic1 Publication
Metal bindingi105 – 1051Magnesium 1; catalytic1 Publication
Metal bindingi105 – 1051Magnesium 2; catalytic1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi58 – 636GTP1 Publication
Nucleotide bindingi104 – 1052GTP1 Publication

GO - Molecular functioni

  • ATP binding Source: BHF-UCL
  • GTP binding Source: BHF-UCL
  • identical protein binding Source: BHF-UCL
  • magnesium ion binding Source: BHF-UCL
  • tRNA binding Source: BHF-UCL
  • tRNA guanylyltransferase activity Source: UniProtKB

GO - Biological processi

  • protein homotetramerization Source: UniProtKB
  • tRNA modification Source: UniProtKB
  • tRNA processing Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.7.79. 2681.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA(His) guanylyltransferase (EC:2.7.7.79)
Alternative name(s):
Interphase cytoplasmic foci protein 45
tRNA-histidine guanylyltransferase
Gene namesi
Name:THG1L
Synonyms:ICF45
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:26053. THG1L.

Subcellular locationi

  • Cytoplasm 1 Publication

  • Note: Found near the nuclear membrane.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi58 – 581D → A: Reduces activity by 99.5%. 1 Publication
Mutagenesisi63 – 631H → A: Slightly reduced enzyme activity. 1 Publication
Mutagenesisi104 – 1041S → A: Slightly reduced enzyme activity. 1 Publication
Mutagenesisi105 – 1051D → A: Loss of enzyme activity. 1 Publication
Mutagenesisi106 – 1061E → A: Reduces activity by 95%. 1 Publication
Mutagenesisi127 – 1271T → A: Abolishes oligomerization. Loss of enzyme activity. 1 Publication
Mutagenesisi181 – 1811H → A: Loss of enzyme activity. 1 Publication
Mutagenesisi216 – 2161K → A: Reduces activity by 98.5%. 1 Publication
Mutagenesisi227 – 2271N → A: Loss of enzyme activity. 1 Publication

Organism-specific databases

PharmGKBiPA162405691.

Polymorphism and mutation databases

BioMutaiTHG1L.
DMDMi146325755.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298Probable tRNA(His) guanylyltransferasePRO_0000284984Add
BLAST

Proteomic databases

MaxQBiQ9NWX6.
PaxDbiQ9NWX6.
PeptideAtlasiQ9NWX6.
PRIDEiQ9NWX6.

PTM databases

PhosphoSiteiQ9NWX6.

Expressioni

Tissue specificityi

Expressed in many tissues.1 Publication

Gene expression databases

BgeeiQ9NWX6.
CleanExiHS_THG1L.
ExpressionAtlasiQ9NWX6. baseline and differential.
GenevisibleiQ9NWX6. HS.

Organism-specific databases

HPAiHPA035877.
HPA035878.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SDCBPO005603EBI-746510,EBI-727004

Protein-protein interaction databases

BioGridi120311. 27 interactions.
DIPiDIP-59479N.
IntActiQ9NWX6. 2 interactions.
MINTiMINT-4873002.
STRINGi9606.ENSP00000231198.

Structurei

Secondary structure

1
298
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi36 – 416Combined sources
Beta strandi51 – 599Combined sources
Helixi62 – 687Combined sources
Helixi77 – 9317Combined sources
Beta strandi94 – 10310Combined sources
Beta strandi106 – 1116Combined sources
Turni117 – 1204Combined sources
Helixi122 – 13918Combined sources
Helixi141 – 1444Combined sources
Beta strandi146 – 1483Combined sources
Beta strandi156 – 16510Combined sources
Helixi166 – 19530Combined sources
Helixi201 – 2088Combined sources
Helixi213 – 22412Combined sources
Helixi228 – 2303Combined sources
Helixi233 – 2375Combined sources
Beta strandi239 – 2435Combined sources
Beta strandi274 – 2774Combined sources
Beta strandi281 – 2833Combined sources
Helixi284 – 2896Combined sources
Helixi292 – 2954Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3OTBX-ray2.95A/B30-298[»]
3OTCX-ray3.01A/B30-298[»]
3OTDX-ray2.28A/B30-298[»]
3OTEX-ray2.56A/B30-298[»]
ProteinModelPortaliQ9NWX6.
SMRiQ9NWX6. Positions 33-296.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NWX6.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG4021.
GeneTreeiENSGT00390000011705.
HOGENOMiHOG000204890.
HOVERGENiHBG054289.
InParanoidiQ9NWX6.
KOiK10761.
OMAiKFEYVRN.
OrthoDBiEOG7X0VHR.
PhylomeDBiQ9NWX6.
TreeFamiTF325119.

Family and domain databases

InterProiIPR025845. Thg1_C_dom.
IPR024956. tRNAHis_GuaTrfase_cat.
IPR007537. tRNAHis_GuaTrfase_Thg1.
[Graphical view]
PANTHERiPTHR12729. PTHR12729. 1 hit.
PfamiPF04446. Thg1. 1 hit.
PF14413. Thg1C. 1 hit.
[Graphical view]
PIRSFiPIRSF028980. tRNAHis_guanylyltransferase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9NWX6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWGACKVKVH DSLATISITL RRYLRLGATM AKSKFEYVRD FEADDTCLAH
60 70 80 90 100
CWVVVRLDGR NFHRFAEKHN FAKPNDSRAL QLMTKCAQTV MEELEDIVIA
110 120 130 140 150
YGQSDEYSFV FKRKTNWFKR RASKFMTHVA SQFASSYVFY WRDYFEDQPL
160 170 180 190 200
LYPPGFDGRV VVYPSNQTLK DYLSWRQADC HINNLYNTVF WALIQQSGLT
210 220 230 240 250
PVQAQGRLQG TLAADKNEIL FSEFNINYNN ELPMYRKGTV LIWQKVDEVM
260 270 280 290
TKEIKLPTEM EGKKMAVTRT RTKPVPLHCD IIGDAFWKEH PEILDEDS
Length:298
Mass (Da):34,831
Last modified:May 1, 2007 - v2
Checksum:iA6C941B75611C448
GO

Sequence cautioni

The sequence AAH01523.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH01852.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti57 – 571L → Q in BAD96839 (Ref. 5) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti232 – 2321L → P.4 Publications
Corresponds to variant rs2270812 [ dbSNP | Ensembl ].
VAR_031871

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY463216 mRNA. Translation: AAS21134.1.
AL136669 mRNA. Translation: CAB66604.1.
AK000553 mRNA. Translation: BAA91249.1.
AK021663 mRNA. Translation: BAB13870.1.
CR533503 mRNA. Translation: CAG38534.1.
AK223119 mRNA. Translation: BAD96839.1.
CH471062 Genomic DNA. Translation: EAW61590.1.
CH471062 Genomic DNA. Translation: EAW61591.1.
BC001523 mRNA. Translation: AAH01523.2. Different initiation.
BC001852 mRNA. Translation: AAH01852.2. Different initiation.
BC023521 mRNA. Translation: AAH23521.1.
CCDSiCCDS4341.1.
RefSeqiNP_060342.2. NM_017872.3.
UniGeneiHs.353090.

Genome annotation databases

EnsembliENST00000231198; ENSP00000231198; ENSG00000113272.
GeneIDi54974.
KEGGihsa:54974.
UCSCiuc003lxd.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY463216 mRNA. Translation: AAS21134.1.
AL136669 mRNA. Translation: CAB66604.1.
AK000553 mRNA. Translation: BAA91249.1.
AK021663 mRNA. Translation: BAB13870.1.
CR533503 mRNA. Translation: CAG38534.1.
AK223119 mRNA. Translation: BAD96839.1.
CH471062 Genomic DNA. Translation: EAW61590.1.
CH471062 Genomic DNA. Translation: EAW61591.1.
BC001523 mRNA. Translation: AAH01523.2. Different initiation.
BC001852 mRNA. Translation: AAH01852.2. Different initiation.
BC023521 mRNA. Translation: AAH23521.1.
CCDSiCCDS4341.1.
RefSeqiNP_060342.2. NM_017872.3.
UniGeneiHs.353090.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3OTBX-ray2.95A/B30-298[»]
3OTCX-ray3.01A/B30-298[»]
3OTDX-ray2.28A/B30-298[»]
3OTEX-ray2.56A/B30-298[»]
ProteinModelPortaliQ9NWX6.
SMRiQ9NWX6. Positions 33-296.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120311. 27 interactions.
DIPiDIP-59479N.
IntActiQ9NWX6. 2 interactions.
MINTiMINT-4873002.
STRINGi9606.ENSP00000231198.

PTM databases

PhosphoSiteiQ9NWX6.

Polymorphism and mutation databases

BioMutaiTHG1L.
DMDMi146325755.

Proteomic databases

MaxQBiQ9NWX6.
PaxDbiQ9NWX6.
PeptideAtlasiQ9NWX6.
PRIDEiQ9NWX6.

Protocols and materials databases

DNASUi54974.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000231198; ENSP00000231198; ENSG00000113272.
GeneIDi54974.
KEGGihsa:54974.
UCSCiuc003lxd.3. human.

Organism-specific databases

CTDi54974.
GeneCardsiGC05P157159.
HGNCiHGNC:26053. THG1L.
HPAiHPA035877.
HPA035878.
neXtProtiNX_Q9NWX6.
PharmGKBiPA162405691.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG4021.
GeneTreeiENSGT00390000011705.
HOGENOMiHOG000204890.
HOVERGENiHBG054289.
InParanoidiQ9NWX6.
KOiK10761.
OMAiKFEYVRN.
OrthoDBiEOG7X0VHR.
PhylomeDBiQ9NWX6.
TreeFamiTF325119.

Enzyme and pathway databases

BRENDAi2.7.7.79. 2681.

Miscellaneous databases

ChiTaRSiTHG1L. human.
EvolutionaryTraceiQ9NWX6.
GeneWikiiTHG1L.
GenomeRNAii54974.
NextBioi58226.
PROiQ9NWX6.

Gene expression databases

BgeeiQ9NWX6.
CleanExiHS_THG1L.
ExpressionAtlasiQ9NWX6. baseline and differential.
GenevisibleiQ9NWX6. HS.

Family and domain databases

InterProiIPR025845. Thg1_C_dom.
IPR024956. tRNAHis_GuaTrfase_cat.
IPR007537. tRNAHis_GuaTrfase_Thg1.
[Graphical view]
PANTHERiPTHR12729. PTHR12729. 1 hit.
PfamiPF04446. Thg1. 1 hit.
PF14413. Thg1C. 1 hit.
[Graphical view]
PIRSFiPIRSF028980. tRNAHis_guanylyltransferase. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of a novel cytoplasm protein ICF45 that is involved in cell cycle regulation."
    Guo D., Hu K., Lei Y., Wang Y., Ma T., He D.
    J. Biol. Chem. 279:53498-53505(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-232.
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-232.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT PRO-232.
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-232.
    Tissue: Brain and Muscle.
  8. "tRNA(His) guanylyltransferase (THG1), a unique 3'-5' nucleotidyl transferase, shares unexpected structural homology with canonical 5'-3' DNA polymerases."
    Hyde S.J., Eckenroth B.E., Smith B.A., Eberley W.A., Heintz N.H., Jackman J.E., Doublie S.
    Proc. Natl. Acad. Sci. U.S.A. 107:20305-20310(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.28 ANGSTROMS) OF 30-298 IN COMPLEX WITH MAGNESIUM; TRIPHOSPHATE AND GTP, FUNCTION, COFACTOR, SUBUNIT, MUTAGENESIS OF ASP-58; HIS-63; SER-104; ASP-105; GLU-106; THR-127; HIS-181; LYS-216 AND ASN-227.

Entry informationi

Entry nameiTHG1_HUMAN
AccessioniPrimary (citable) accession number: Q9NWX6
Secondary accession number(s): D3DQJ5
, Q53G12, Q7L5R3, Q9H0S2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: July 22, 2015
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.