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Q9NWX6

- THG1_HUMAN

UniProt

Q9NWX6 - THG1_HUMAN

Protein

Probable tRNA(His) guanylyltransferase

Gene

THG1L

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (01 May 2007)
      Previous versions | rss
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    Functioni

    Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. This step is essential for proper recognition of the tRNA and for the fidelity of protein synthesis.1 Publication

    Catalytic activityi

    p-tRNA(His) + ATP + GTP = pppG-P-tRNA(His) + AMP + diphosphate.

    Cofactori

    Binds 2 magnesium ions per subunit.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi58 – 581Magnesium 1; catalytic1 Publication
    Metal bindingi58 – 581Magnesium 2; catalytic1 Publication
    Metal bindingi59 – 591Magnesium 1; via carbonyl oxygen; catalytic1 Publication
    Metal bindingi105 – 1051Magnesium 1; catalytic1 Publication
    Metal bindingi105 – 1051Magnesium 2; catalytic1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi58 – 636GTP1 Publication
    Nucleotide bindingi104 – 1052GTP1 Publication

    GO - Molecular functioni

    1. ATP binding Source: BHF-UCL
    2. GTP binding Source: BHF-UCL
    3. identical protein binding Source: BHF-UCL
    4. magnesium ion binding Source: BHF-UCL
    5. tRNA binding Source: BHF-UCL
    6. tRNA guanylyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. protein homotetramerization Source: UniProtKB
    2. tRNA modification Source: UniProtKB
    3. tRNA processing Source: UniProtKB

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable tRNA(His) guanylyltransferase (EC:2.7.7.79)
    Alternative name(s):
    Interphase cytoplasmic foci protein 45
    tRNA-histidine guanylyltransferase
    Gene namesi
    Name:THG1L
    Synonyms:ICF45
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:26053. THG1L.

    Subcellular locationi

    Cytoplasm 1 Publication
    Note: Found near the nuclear membrane.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. mitochondrion Source: LIFEdb

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi58 – 581D → A: Reduces activity by 99.5%. 1 Publication
    Mutagenesisi63 – 631H → A: Slightly reduced enzyme activity. 1 Publication
    Mutagenesisi104 – 1041S → A: Slightly reduced enzyme activity. 1 Publication
    Mutagenesisi105 – 1051D → A: Loss of enzyme activity. 1 Publication
    Mutagenesisi106 – 1061E → A: Reduces activity by 95%. 1 Publication
    Mutagenesisi127 – 1271T → A: Abolishes oligomerization. Loss of enzyme activity. 1 Publication
    Mutagenesisi181 – 1811H → A: Loss of enzyme activity. 1 Publication
    Mutagenesisi216 – 2161K → A: Reduces activity by 98.5%. 1 Publication
    Mutagenesisi227 – 2271N → A: Loss of enzyme activity. 1 Publication

    Organism-specific databases

    PharmGKBiPA162405691.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 298298Probable tRNA(His) guanylyltransferasePRO_0000284984Add
    BLAST

    Proteomic databases

    MaxQBiQ9NWX6.
    PaxDbiQ9NWX6.
    PeptideAtlasiQ9NWX6.
    PRIDEiQ9NWX6.

    PTM databases

    PhosphoSiteiQ9NWX6.

    Expressioni

    Tissue specificityi

    Expressed in many tissues.1 Publication

    Gene expression databases

    ArrayExpressiQ9NWX6.
    BgeeiQ9NWX6.
    CleanExiHS_THG1L.
    GenevestigatoriQ9NWX6.

    Organism-specific databases

    HPAiHPA035877.
    HPA035878.

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    BioGridi120311. 23 interactions.
    DIPiDIP-59479N.
    IntActiQ9NWX6. 1 interaction.
    MINTiMINT-4873002.
    STRINGi9606.ENSP00000231198.

    Structurei

    Secondary structure

    1
    298
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi36 – 416
    Beta strandi51 – 599
    Helixi62 – 687
    Helixi77 – 9317
    Beta strandi94 – 10310
    Beta strandi106 – 1116
    Turni117 – 1204
    Helixi122 – 13918
    Helixi141 – 1444
    Beta strandi146 – 1483
    Beta strandi156 – 16510
    Helixi166 – 19530
    Helixi201 – 2088
    Helixi213 – 22412
    Helixi228 – 2303
    Helixi233 – 2375
    Beta strandi239 – 2435
    Beta strandi274 – 2774
    Beta strandi281 – 2833
    Helixi284 – 2896
    Helixi292 – 2954

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3OTBX-ray2.95A/B30-298[»]
    3OTCX-ray3.01A/B30-298[»]
    3OTDX-ray2.28A/B30-298[»]
    3OTEX-ray2.56A/B30-298[»]
    ProteinModelPortaliQ9NWX6.
    SMRiQ9NWX6. Positions 33-296.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9NWX6.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG4021.
    HOGENOMiHOG000204890.
    HOVERGENiHBG054289.
    InParanoidiQ9NWX6.
    KOiK10761.
    OMAiKFEYVRN.
    OrthoDBiEOG7X0VHR.
    PhylomeDBiQ9NWX6.
    TreeFamiTF325119.

    Family and domain databases

    InterProiIPR025845. Thg1_C_dom.
    IPR024956. tRNAHis_GuaTrfase_cat.
    IPR007537. tRNAHis_GuaTrfase_Thg1.
    [Graphical view]
    PANTHERiPTHR12729. PTHR12729. 1 hit.
    PfamiPF04446. Thg1. 1 hit.
    PF14413. Thg1C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF028980. tRNAHis_guanylyltransferase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9NWX6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWGACKVKVH DSLATISITL RRYLRLGATM AKSKFEYVRD FEADDTCLAH    50
    CWVVVRLDGR NFHRFAEKHN FAKPNDSRAL QLMTKCAQTV MEELEDIVIA 100
    YGQSDEYSFV FKRKTNWFKR RASKFMTHVA SQFASSYVFY WRDYFEDQPL 150
    LYPPGFDGRV VVYPSNQTLK DYLSWRQADC HINNLYNTVF WALIQQSGLT 200
    PVQAQGRLQG TLAADKNEIL FSEFNINYNN ELPMYRKGTV LIWQKVDEVM 250
    TKEIKLPTEM EGKKMAVTRT RTKPVPLHCD IIGDAFWKEH PEILDEDS 298
    Length:298
    Mass (Da):34,831
    Last modified:May 1, 2007 - v2
    Checksum:iA6C941B75611C448
    GO

    Sequence cautioni

    The sequence AAH01523.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence AAH01852.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti57 – 571L → Q in BAD96839. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti232 – 2321L → P.4 Publications
    Corresponds to variant rs2270812 [ dbSNP | Ensembl ].
    VAR_031871

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY463216 mRNA. Translation: AAS21134.1.
    AL136669 mRNA. Translation: CAB66604.1.
    AK000553 mRNA. Translation: BAA91249.1.
    AK021663 mRNA. Translation: BAB13870.1.
    CR533503 mRNA. Translation: CAG38534.1.
    AK223119 mRNA. Translation: BAD96839.1.
    CH471062 Genomic DNA. Translation: EAW61590.1.
    CH471062 Genomic DNA. Translation: EAW61591.1.
    BC001523 mRNA. Translation: AAH01523.2. Different initiation.
    BC001852 mRNA. Translation: AAH01852.2. Different initiation.
    BC023521 mRNA. Translation: AAH23521.1.
    CCDSiCCDS4341.1.
    RefSeqiNP_060342.2. NM_017872.3.
    UniGeneiHs.353090.

    Genome annotation databases

    EnsembliENST00000231198; ENSP00000231198; ENSG00000113272.
    GeneIDi54974.
    KEGGihsa:54974.
    UCSCiuc003lxd.3. human.

    Polymorphism databases

    DMDMi146325755.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY463216 mRNA. Translation: AAS21134.1 .
    AL136669 mRNA. Translation: CAB66604.1 .
    AK000553 mRNA. Translation: BAA91249.1 .
    AK021663 mRNA. Translation: BAB13870.1 .
    CR533503 mRNA. Translation: CAG38534.1 .
    AK223119 mRNA. Translation: BAD96839.1 .
    CH471062 Genomic DNA. Translation: EAW61590.1 .
    CH471062 Genomic DNA. Translation: EAW61591.1 .
    BC001523 mRNA. Translation: AAH01523.2 . Different initiation.
    BC001852 mRNA. Translation: AAH01852.2 . Different initiation.
    BC023521 mRNA. Translation: AAH23521.1 .
    CCDSi CCDS4341.1.
    RefSeqi NP_060342.2. NM_017872.3.
    UniGenei Hs.353090.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3OTB X-ray 2.95 A/B 30-298 [» ]
    3OTC X-ray 3.01 A/B 30-298 [» ]
    3OTD X-ray 2.28 A/B 30-298 [» ]
    3OTE X-ray 2.56 A/B 30-298 [» ]
    ProteinModelPortali Q9NWX6.
    SMRi Q9NWX6. Positions 33-296.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 120311. 23 interactions.
    DIPi DIP-59479N.
    IntActi Q9NWX6. 1 interaction.
    MINTi MINT-4873002.
    STRINGi 9606.ENSP00000231198.

    PTM databases

    PhosphoSitei Q9NWX6.

    Polymorphism databases

    DMDMi 146325755.

    Proteomic databases

    MaxQBi Q9NWX6.
    PaxDbi Q9NWX6.
    PeptideAtlasi Q9NWX6.
    PRIDEi Q9NWX6.

    Protocols and materials databases

    DNASUi 54974.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000231198 ; ENSP00000231198 ; ENSG00000113272 .
    GeneIDi 54974.
    KEGGi hsa:54974.
    UCSCi uc003lxd.3. human.

    Organism-specific databases

    CTDi 54974.
    GeneCardsi GC05P157159.
    HGNCi HGNC:26053. THG1L.
    HPAi HPA035877.
    HPA035878.
    neXtProti NX_Q9NWX6.
    PharmGKBi PA162405691.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG4021.
    HOGENOMi HOG000204890.
    HOVERGENi HBG054289.
    InParanoidi Q9NWX6.
    KOi K10761.
    OMAi KFEYVRN.
    OrthoDBi EOG7X0VHR.
    PhylomeDBi Q9NWX6.
    TreeFami TF325119.

    Miscellaneous databases

    ChiTaRSi THG1L. human.
    EvolutionaryTracei Q9NWX6.
    GeneWikii THG1L.
    GenomeRNAii 54974.
    NextBioi 58226.
    PROi Q9NWX6.

    Gene expression databases

    ArrayExpressi Q9NWX6.
    Bgeei Q9NWX6.
    CleanExi HS_THG1L.
    Genevestigatori Q9NWX6.

    Family and domain databases

    InterProi IPR025845. Thg1_C_dom.
    IPR024956. tRNAHis_GuaTrfase_cat.
    IPR007537. tRNAHis_GuaTrfase_Thg1.
    [Graphical view ]
    PANTHERi PTHR12729. PTHR12729. 1 hit.
    Pfami PF04446. Thg1. 1 hit.
    PF14413. Thg1C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF028980. tRNAHis_guanylyltransferase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of a novel cytoplasm protein ICF45 that is involved in cell cycle regulation."
      Guo D., Hu K., Lei Y., Wang Y., Ma T., He D.
      J. Biol. Chem. 279:53498-53505(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-232.
    4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
      Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-232.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT PRO-232.
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-232.
      Tissue: Brain and Muscle.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "tRNA(His) guanylyltransferase (THG1), a unique 3'-5' nucleotidyl transferase, shares unexpected structural homology with canonical 5'-3' DNA polymerases."
      Hyde S.J., Eckenroth B.E., Smith B.A., Eberley W.A., Heintz N.H., Jackman J.E., Doublie S.
      Proc. Natl. Acad. Sci. U.S.A. 107:20305-20310(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.28 ANGSTROMS) OF 30-298 IN COMPLEX WITH MAGNESIUM; TRIPHOSPHATE AND GTP, FUNCTION, COFACTOR, SUBUNIT, MUTAGENESIS OF ASP-58; HIS-63; SER-104; ASP-105; GLU-106; THR-127; HIS-181; LYS-216 AND ASN-227.

    Entry informationi

    Entry nameiTHG1_HUMAN
    AccessioniPrimary (citable) accession number: Q9NWX6
    Secondary accession number(s): D3DQJ5
    , Q53G12, Q7L5R3, Q9H0S2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 2007
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3