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Protein

HRAS-like suppressor 2

Gene

HRASLS2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Exhibits PLA1/2 activity, catalyzing the calcium-independent hydrolysis of acyl groups in various phosphatidylcholines (PC) and phosphatidylethanolamine (PE). For most substrates, PLA1 activity is much higher than PLA2 activity. Catalyzes N-acylation of PE using both sn-1 and sn-2 palmitoyl groups of PC as acyl donor. Also catalyzes O-acylation converting lyso-PC into PC.1 Publication

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.1 Publication
Phosphatidylcholine + H2O = 2-acylglycerophosphocholine + a carboxylate.1 Publication
Phosphatidylcholine + phosphoethanolamine = 1-acylglycerophosphocholine + N-palmitoyl-phosphoethanolamine.1 Publication

Kineticsi

  1. KM=300 µM for dipalmitoyl-PC1 Publication
  1. Vmax=670 nmol/min/mg enzyme with dipalmitoyl-PC as substrate1 Publication
  2. Vmax=122 nmol/min/mg enzyme with dipalmitoyl-PE as substrate1 Publication
  3. Vmax=103 nmol/min/mg enzyme using dipalmitoyl-PC as an acyl donor and PE as an acyl acceptor1 Publication

pH dependencei

Optimum pH is 8.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei23 – 2311 Publication
Active sitei35 – 3511 Publication
Active sitei113 – 1131Acyl-thioester intermediate1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Hydrolase, Transferase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BRENDAi2.7.1.22. 2681.

Chemistry

SwissLipidsiSLP:000001075.

Names & Taxonomyi

Protein namesi
Recommended name:
HRAS-like suppressor 2 (EC:2.3.1.-, EC:3.1.1.-)
Gene namesi
Name:HRASLS2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:17824. HRASLS2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 132132CytoplasmicSequence analysisAdd
BLAST
Transmembranei133 – 15523HelicalSequence analysisAdd
BLAST
Topological domaini156 – 1627LumenalSequence analysis

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29446.

Polymorphism and mutation databases

BioMutaiHRASLS2.
DMDMi23396611.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 162162HRAS-like suppressor 2PRO_0000152483Add
BLAST

Proteomic databases

PaxDbiQ9NWW9.
PeptideAtlasiQ9NWW9.
PRIDEiQ9NWW9.

PTM databases

iPTMnetiQ9NWW9.
PhosphoSiteiQ9NWW9.

Expressioni

Tissue specificityi

Expressed in liver, kidney, small intestine testis and colon (PubMed:19615464). Undetectable in testis, placenta, salivary gland and fetal brain (PubMed:18163183).2 Publications

Gene expression databases

BgeeiENSG00000133328.
CleanExiHS_HRASLS2.
GenevisibleiQ9NWW9. HS.

Organism-specific databases

HPAiHPA058997.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000255695.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4DPZX-ray1.25X1-129[»]
ProteinModelPortaliQ9NWW9.
SMRiQ9NWW9. Positions 6-125.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the H-rev107 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410JBZ4. Eukaryota.
ENOG4111M0Q. LUCA.
GeneTreeiENSGT00390000013327.
HOGENOMiHOG000293227.
HOVERGENiHBG028837.
InParanoidiQ9NWW9.
OMAiFGYAHWA.
OrthoDBiEOG091G08MK.
PhylomeDBiQ9NWW9.
TreeFamiTF330836.

Family and domain databases

InterProiIPR007053. LRAT-like_dom.
[Graphical view]
PfamiPF04970. LRAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9NWW9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALARPRPRL GDLIEISRFG YAHWAIYVGD GYVVHLAPAS EIAGAGAASV
60 70 80 90 100
LSALTNKAIV KKELLSVVAG GDNYRVNNKH DDRYTPLPSN KIVKRAEELV
110 120 130 140 150
GQELPYSLTS DNCEHFVNHL RYGVSRSDQV TGAVTTVGVA AGLLAAASLV
160
GILLARSKRE RQ
Length:162
Mass (Da):17,394
Last modified:October 1, 2000 - v1
Checksum:iBF89C10698D4F1D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB453251 mRNA. Translation: BAH22445.1.
AK000563 mRNA. Translation: BAA91256.1.
CH471076 Genomic DNA. Translation: EAW74157.1.
CCDSiCCDS8046.1.
RefSeqiNP_060348.1. NM_017878.1.
UniGeneiHs.272805.

Genome annotation databases

EnsembliENST00000255695; ENSP00000255695; ENSG00000133328.
GeneIDi54979.
KEGGihsa:54979.
UCSCiuc001nxg.1. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB453251 mRNA. Translation: BAH22445.1.
AK000563 mRNA. Translation: BAA91256.1.
CH471076 Genomic DNA. Translation: EAW74157.1.
CCDSiCCDS8046.1.
RefSeqiNP_060348.1. NM_017878.1.
UniGeneiHs.272805.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4DPZX-ray1.25X1-129[»]
ProteinModelPortaliQ9NWW9.
SMRiQ9NWW9. Positions 6-125.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000255695.

Chemistry

SwissLipidsiSLP:000001075.

PTM databases

iPTMnetiQ9NWW9.
PhosphoSiteiQ9NWW9.

Polymorphism and mutation databases

BioMutaiHRASLS2.
DMDMi23396611.

Proteomic databases

PaxDbiQ9NWW9.
PeptideAtlasiQ9NWW9.
PRIDEiQ9NWW9.

Protocols and materials databases

DNASUi54979.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000255695; ENSP00000255695; ENSG00000133328.
GeneIDi54979.
KEGGihsa:54979.
UCSCiuc001nxg.1. human.

Organism-specific databases

CTDi54979.
GeneCardsiHRASLS2.
HGNCiHGNC:17824. HRASLS2.
HPAiHPA058997.
MIMi613866. gene.
neXtProtiNX_Q9NWW9.
PharmGKBiPA29446.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410JBZ4. Eukaryota.
ENOG4111M0Q. LUCA.
GeneTreeiENSGT00390000013327.
HOGENOMiHOG000293227.
HOVERGENiHBG028837.
InParanoidiQ9NWW9.
OMAiFGYAHWA.
OrthoDBiEOG091G08MK.
PhylomeDBiQ9NWW9.
TreeFamiTF330836.

Enzyme and pathway databases

BRENDAi2.7.1.22. 2681.

Miscellaneous databases

GenomeRNAii54979.
PROiQ9NWW9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000133328.
CleanExiHS_HRASLS2.
GenevisibleiQ9NWW9. HS.

Family and domain databases

InterProiIPR007053. LRAT-like_dom.
[Graphical view]
PfamiPF04970. LRAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHRSL2_HUMAN
AccessioniPrimary (citable) accession number: Q9NWW9
Secondary accession number(s): B9A7L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.