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Protein

Mediator of RNA polymerase II transcription subunit 9

Gene

MED9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-1989781. PPARA activates gene expression.
R-HSA-381340. Transcriptional regulation of white adipocyte differentiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 9
Alternative name(s):
Mediator complex subunit 9
Gene namesi
Name:MED9
Synonyms:MED25
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:25487. MED9.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134925396.

Polymorphism and mutation databases

BioMutaiMED9.
DMDMi74734632.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 146145Mediator of RNA polymerase II transcription subunit 9PRO_0000304147Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources
Modified residuei110 – 1101PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9NWA0.
MaxQBiQ9NWA0.
PaxDbiQ9NWA0.
PeptideAtlasiQ9NWA0.
PRIDEiQ9NWA0.

PTM databases

iPTMnetiQ9NWA0.
PhosphoSiteiQ9NWA0.

Expressioni

Gene expression databases

BgeeiQ9NWA0.
CleanExiHS_MED25.
HS_MED9.
ExpressionAtlasiQ9NWA0. baseline and differential.
GenevisibleiQ9NWA0. HS.

Organism-specific databases

HPAiHPA056415.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MED21Q135033EBI-394653,EBI-394678
MED4Q9NPJ610EBI-394653,EBI-394607

Protein-protein interaction databases

BioGridi120403. 51 interactions.
IntActiQ9NWA0. 70 interactions.
STRINGi9606.ENSP00000268711.

Structurei

3D structure databases

ProteinModelPortaliQ9NWA0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili112 – 13928Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi17 – 5438Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the Mediator complex subunit 9 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410J0JM. Eukaryota.
ENOG4111U35. LUCA.
GeneTreeiENSGT00390000017379.
HOGENOMiHOG000113551.
HOVERGENiHBG105579.
InParanoidiQ9NWA0.
KOiK15149.
OMAiNCSFLPL.
OrthoDBiEOG7JHM80.
PhylomeDBiQ9NWA0.
TreeFamiTF324926.

Family and domain databases

InterProiIPR011425. Med9.
[Graphical view]
PfamiPF07544. Med9. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9NWA0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASAGVAAGR QAEDVLPPTS DQPLPDTKPL PPPQPPPVPA PQPQQSPAPR
60 70 80 90 100
PQSPARAREE ENYSFLPLVH NIIKCMDKDS PEVHQDLNAL KSKFQEMRKL
110 120 130 140
ISTMPGIHLS PEQQQQQLQS LREQVRTKNE LLQKYKSLCM FEIPKE
Length:146
Mass (Da):16,403
Last modified:October 1, 2000 - v1
Checksum:i24CDB7CBDFD36D1A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001055 mRNA. Translation: BAA91483.1.
CH471196 Genomic DNA. Translation: EAW55706.1.
BC104988 mRNA. Translation: AAI04989.1.
BC112029 mRNA. Translation: AAI12030.1.
CCDSiCCDS11184.1.
RefSeqiNP_060489.1. NM_018019.2.
UniGeneiHs.244595.

Genome annotation databases

EnsembliENST00000268711; ENSP00000268711; ENSG00000141026.
GeneIDi55090.
KEGGihsa:55090.
UCSCiuc002grh.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001055 mRNA. Translation: BAA91483.1.
CH471196 Genomic DNA. Translation: EAW55706.1.
BC104988 mRNA. Translation: AAI04989.1.
BC112029 mRNA. Translation: AAI12030.1.
CCDSiCCDS11184.1.
RefSeqiNP_060489.1. NM_018019.2.
UniGeneiHs.244595.

3D structure databases

ProteinModelPortaliQ9NWA0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120403. 51 interactions.
IntActiQ9NWA0. 70 interactions.
STRINGi9606.ENSP00000268711.

PTM databases

iPTMnetiQ9NWA0.
PhosphoSiteiQ9NWA0.

Polymorphism and mutation databases

BioMutaiMED9.
DMDMi74734632.

Proteomic databases

EPDiQ9NWA0.
MaxQBiQ9NWA0.
PaxDbiQ9NWA0.
PeptideAtlasiQ9NWA0.
PRIDEiQ9NWA0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000268711; ENSP00000268711; ENSG00000141026.
GeneIDi55090.
KEGGihsa:55090.
UCSCiuc002grh.2. human.

Organism-specific databases

CTDi55090.
GeneCardsiMED9.
HGNCiHGNC:25487. MED9.
HPAiHPA056415.
MIMi609878. gene.
neXtProtiNX_Q9NWA0.
PharmGKBiPA134925396.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410J0JM. Eukaryota.
ENOG4111U35. LUCA.
GeneTreeiENSGT00390000017379.
HOGENOMiHOG000113551.
HOVERGENiHBG105579.
InParanoidiQ9NWA0.
KOiK15149.
OMAiNCSFLPL.
OrthoDBiEOG7JHM80.
PhylomeDBiQ9NWA0.
TreeFamiTF324926.

Enzyme and pathway databases

ReactomeiR-HSA-1989781. PPARA activates gene expression.
R-HSA-381340. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

ChiTaRSiMED9. human.
GenomeRNAii55090.
PROiQ9NWA0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9NWA0.
CleanExiHS_MED25.
HS_MED9.
ExpressionAtlasiQ9NWA0. baseline and differential.
GenevisibleiQ9NWA0. HS.

Family and domain databases

InterProiIPR011425. Med9.
[Graphical view]
PfamiPF07544. Med9. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
    Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
    Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
  5. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
    Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
    Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
  6. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Toward a comprehensive characterization of a human cancer cell phosphoproteome."
    Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J., Mohammed S.
    J. Proteome Res. 12:260-271(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Erythroleukemia.

Entry informationi

Entry nameiMED9_HUMAN
AccessioniPrimary (citable) accession number: Q9NWA0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: October 1, 2000
Last modified: July 6, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.