Reviewed, UniProtKB/Swiss-Prot Q9NW15 (ANO10_HUMAN)

Names and origin

Protein names

Recommended name:
    Anoctamin-10
Alternative name(s):
    Transmembrane protein 16K

Gene names

Name:	ANO10
Synonyms:	TMEM16K

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	660 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May act as a calcium-activated chloride channel.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the anoctamin family.
Sequence caution	The sequence BAA91573.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. The sequence BC038855 differs from that shown. Reason: Frameshift at position 42.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane
Ligand	Calcium Chloride
Molecular function	Chloride channel Ionic channel
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloride channel complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW chloride channel activity Inferred from electronic annotation. Source: UniProtKB-KW chloride ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NW15-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NW15-2)

The sequence of this isoform differs from the canonical sequence as follows:
     198-387: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9NW15-3)

The sequence of this isoform differs from the canonical sequence as follows:
     47-112: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q9NW15-4)

The sequence of this isoform differs from the canonical sequence as follows:
     47-158: GAQLLFRPLL...AKLYPGKSLL → V

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 660	660	Anoctamin-10		PRO_0000289957
Regions
	Topological domain	1 – 207	207	Cytoplasmic Potential
	Transmembrane	208 – 228	21	Potential
	Topological domain	229 – 240	12	Extracellular Potential
	Transmembrane	241 – 261	21	Potential
	Topological domain	262 – 316	55	Cytoplasmic Potential
	Transmembrane	317 – 337	21	Potential
	Topological domain	338 – 352	15	Extracellular Potential
	Transmembrane	353 – 373	21	Potential
	Topological domain	374 – 400	27	Cytoplasmic Potential
	Transmembrane	401 – 421	21	Potential
	Topological domain	422 – 500	79	Extracellular Potential
	Transmembrane	501 – 521	21	Potential
	Topological domain	522 – 553	32	Cytoplasmic Potential
	Transmembrane	554 – 574	21	Potential
	Topological domain	575 – 590	16	Extracellular Potential
	Transmembrane	591 – 611	21	Potential
	Topological domain	612 – 660	49	Cytoplasmic Potential
Natural variations
	Alternative sequence	47 – 158	112	GAQLL…GKSLL → V in isoform 4.		VSP_038211
	Alternative sequence	47 – 112	66	Missing in isoform 3.		VSP_038212
	Alternative sequence	198 – 387	190	Missing in isoform 2.		VSP_026033
	Natural variant	462	1	R → Q: dbSNP rs3772165. Ref.1		VAR_032638
	Natural variant	561	1	T → M: dbSNP rs17409162. Ref.3		VAR_032639
	Natural variant	583	1	V → A: dbSNP rs17853862. Ref.3		VAR_032640

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 29, 2007. Version 2. Checksum: 21582D364497ADFD	FASTA	660	76,329
	10 20 30 40 50 60 MKVTLSALDT SESSFTPLVV IELAQDVKEE TKEWLKNRII AKKKDGGAQL LFRPLLNKYE 70 80 90 100 110 120 QETLENQNLY LVGASKIRML LGAEAVGLVK ECNDNTMRAF TYRTRQNFKG FDDNNDDFLT 130 140 150 160 170 180 MAECQFIIKH ELENLRAKDE KMIPGYPQAK LYPGKSLLRR LLTSGIVIQV FPLHDSEALK 190 200 210 220 230 240 KLEDTWYTRF ALKYQPIDSI RGYFGETIAL YFGFLEYFTF ALIPMAVIGL PYYLFVWEDY 250 260 270 280 290 300 DKYVIFASFN LIWSTVILEL WKRGCANMTY RWGTLLMKRK FEEPRPGFHG VLGINSITGK 310 320 330 340 350 360 EEPLYPSYKR QLRIYLVSLP FVCLCLYFSL YVMMIYFDME VWALGLHENS GSEWTSVLLY 370 380 390 400 410 420 VPSIIYAIVI EIMNRLYRYA AEFLTSWENH RLESAYQNHL ILKVLVFNFL NCFASLFYIA 430 440 450 460 470 480 FVLKDMKLLR QSLATLLITS QILNQIMESF LPYWLQRKHG VRVKRKVQAL KADIDATLYE 490 500 510 520 530 540 QVILEKEMGT YLGTFDDYLE LFLQFGYVSL FSCVYPLAAA FAVLNNFTEV NSDALKMCRV 550 560 570 580 590 600 FKRPFSEPSA NIGVWQLAFE TMSVISVVTN CALIGMSPQV NAVFPESKAD LILIVVAVEH 610 620 630 640 650 660 ALLALKFILA FAIPDKPRHI QMKLARLEFE SLEALKQQQM KLVTENLKEE PMESGKEKAT
	Isoform 2. Checksum: E19D897F9DE194AE	FASTA	470	53,843
	10 20 30 40 50 60 MKVTLSALDT SESSFTPLVV IELAQDVKEE TKEWLKNRII AKKKDGGAQL LFRPLLNKYE 70 80 90 100 110 120 QETLENQNLY LVGASKIRML LGAEAVGLVK ECNDNTMRAF TYRTRQNFKG FDDNNDDFLT 130 140 150 160 170 180 MAECQFIIKH ELENLRAKDE KMIPGYPQAK LYPGKSLLRR LLTSGIVIQV FPLHDSEALK 190 200 210 220 230 240 KLEDTWYTRF ALKYQPIENH RLESAYQNHL ILKVLVFNFL NCFASLFYIA FVLKDMKLLR 250 260 270 280 290 300 QSLATLLITS QILNQIMESF LPYWLQRKHG VRVKRKVQAL KADIDATLYE QVILEKEMGT 310 320 330 340 350 360 YLGTFDDYLE LFLQFGYVSL FSCVYPLAAA FAVLNNFTEV NSDALKMCRV FKRPFSEPSA 370 380 390 400 410 420 NIGVWQLAFE TMSVISVVTN CALIGMSPQV NAVFPESKAD LILIVVAVEH ALLALKFILA 430 440 450 460 470 FAIPDKPRHI QMKLARLEFE SLEALKQQQM KLVTENLKEE PMESGKEKAT
	Isoform 3. Checksum: C9DEE8503AC34E78	FASTA	594	68,748
	10 20 30 40 50 60 MKVTLSALDT SESSFTPLVV IELAQDVKEE TKEWLKNRII AKKKDGDNND DFLTMAECQF 70 80 90 100 110 120 IIKHELENLR AKDEKMIPGY PQAKLYPGKS LLRRLLTSGI VIQVFPLHDS EALKKLEDTW 130 140 150 160 170 180 YTRFALKYQP IDSIRGYFGE TIALYFGFLE YFTFALIPMA VIGLPYYLFV WEDYDKYVIF 190 200 210 220 230 240 ASFNLIWSTV ILELWKRGCA NMTYRWGTLL MKRKFEEPRP GFHGVLGINS ITGKEEPLYP 250 260 270 280 290 300 SYKRQLRIYL VSLPFVCLCL YFSLYVMMIY FDMEVWALGL HENSGSEWTS VLLYVPSIIY 310 320 330 340 350 360 AIVIEIMNRL YRYAAEFLTS WENHRLESAY QNHLILKVLV FNFLNCFASL FYIAFVLKDM 370 380 390 400 410 420 KLLRQSLATL LITSQILNQI MESFLPYWLQ RKHGVRVKRK VQALKADIDA TLYEQVILEK 430 440 450 460 470 480 EMGTYLGTFD DYLELFLQFG YVSLFSCVYP LAAAFAVLNN FTEVNSDALK MCRVFKRPFS 490 500 510 520 530 540 EPSANIGVWQ LAFETMSVIS VVTNCALIGM SPQVNAVFPE SKADLILIVV AVEHALLALK 550 560 570 580 590 FILAFAIPDK PRHIQMKLAR LEFESLEALK QQQMKLVTEN LKEEPMESGK EKAT
	Isoform 4. Checksum: 2BDE5BF63C2BD6E0	FASTA	549	63,526
	10 20 30 40 50 60 MKVTLSALDT SESSFTPLVV IELAQDVKEE TKEWLKNRII AKKKDGVRRL LTSGIVIQVF 70 80 90 100 110 120 PLHDSEALKK LEDTWYTRFA LKYQPIDSIR GYFGETIALY FGFLEYFTFA LIPMAVIGLP 130 140 150 160 170 180 YYLFVWEDYD KYVIFASFNL IWSTVILELW KRGCANMTYR WGTLLMKRKF EEPRPGFHGV 190 200 210 220 230 240 LGINSITGKE EPLYPSYKRQ LRIYLVSLPF VCLCLYFSLY VMMIYFDMEV WALGLHENSG 250 260 270 280 290 300 SEWTSVLLYV PSIIYAIVIE IMNRLYRYAA EFLTSWENHR LESAYQNHLI LKVLVFNFLN 310 320 330 340 350 360 CFASLFYIAF VLKDMKLLRQ SLATLLITSQ ILNQIMESFL PYWLQRKHGV RVKRKVQALK 370 380 390 400 410 420 ADIDATLYEQ VILEKEMGTY LGTFDDYLEL FLQFGYVSLF SCVYPLAAAF AVLNNFTEVN 430 440 450 460 470 480 SDALKMCRVF KRPFSEPSAN IGVWQLAFET MSVISVVTNC ALIGMSPQVN AVFPESKADL 490 500 510 520 530 540 ILIVVAVEHA LLALKFILAF AIPDKPRHIQ MKLARLEFES LEALKQQQMK LVTENLKEEP MESGKEKAT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 3 AND 4), VARIANT GLN-462. Tissue: Substantia nigra, Teratocarcinoma, Testis and Urinary bladder.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS MET-561 AND ALA-583. Tissue: Leukocyte.
[4]	"TMEM16A confers receptor-activated calcium-dependent chloride conductance." Yang Y.D., Cho H., Koo J.Y., Tak M.H., Cho Y., Shim W.-S., Park S.P., Lee J., Lee B., Kim B.-M., Raouf R., Shin Y.K., Oh U. Nature 455:1210-1215(2008) [PubMed: 18724360] [Abstract] Cited for: IDENTIFICATION AS PUTATIVE CHLORIDE CHANNEL.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK001237 mRNA. Translation: BAA91573.1. Sequence problems. AK096302 mRNA. Translation: BAG53253.1. AK131223 mRNA. Translation: BAG54755.1. AK292368 mRNA. Translation: BAF85057.1. AK295969 mRNA. Translation: BAG58745.1. CH471055 Genomic DNA. Translation: EAW64696.1. BC038855 mRNA. No translation available.
IPI	IPI00794169. IPI00797182. IPI00908702. IPI00941544.
RefSeq	NP_060545.3.
UniGene	Hs.656657
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9NW15.
Genome annotation databases
Ensembl	ENST00000292246; ENSP00000292246; ENSG00000160746; Homo sapiens. [Genome view] ENST00000396092; ENSP00000379399; ENSG00000160746; Homo sapiens. [Genome view] ENST00000418371; ENSP00000391537; ENSG00000160746; Homo sapiens. [Genome view]
GeneID	55129.
KEGG	hsa:55129.
NMPDR	fig|9606.3.peg.22368.
UCSC	uc003cmv.1. human.
Organism-specific databases
CTD	55129.
GeneCards	GC03M043385.
HGNC	HGNC:25519. ANO10.
PharmGKB	PA142670790.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9NW15.
HOVERGEN	Q9NW15.
OMA	HGVLGIN
OrthoDB	EOG94F8VN
Gene expression databases
ArrayExpress	Q9NW15.
Bgee	Q9NW15.
CleanEx	HS_ANO10.
Genevestigator	Q9NW15.
Family and domain databases
InterPro	IPR007632. DUF590. [Graphical view]
PANTHER	PTHR12308. DUF590. 1 hit.
Pfam	PF04547. DUF590. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	58794.

Entry information

Entry name

ANO10_HUMAN

Accession

Primary (citable) accession number: Q9NW15
Secondary accession number(s): A8K8K3 , B3KTZ1, B3KY93, B4DJ83, Q8IXX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	May 29, 2007
Last modified:	November 24, 2009
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.