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Protein

DNA-directed RNA polymerase III subunit RPC2

Gene

POLR3B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.By similarity2 Publications

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi1080ZincBy similarity1
Metal bindingi1083ZincBy similarity1
Metal bindingi1092ZincBy similarity1
Metal bindingi1095ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1080 – 1095C4-typeAdd BLAST16

GO - Molecular functioni

GO - Biological processi

  • defense response to virus Source: UniProtKB-KW
  • innate immune response Source: UniProtKB-KW
  • positive regulation of innate immune response Source: UniProtKB
  • positive regulation of interferon-beta production Source: UniProtKB
  • positive regulation of type I interferon production Source: Reactome
  • transcription from RNA polymerase III promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Antiviral defense, Immunity, Innate immunity, Transcription

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:HS12049-MONOMER.
ReactomeiR-HSA-1834949. Cytosolic sensors of pathogen-associated DNA.
R-HSA-73780. RNA Polymerase III Chain Elongation.
R-HSA-73980. RNA Polymerase III Transcription Termination.
R-HSA-749476. RNA Polymerase III Abortive And Retractive Initiation.
R-HSA-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-HSA-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-HSA-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase III subunit RPC2 (EC:2.7.7.6)
Short name:
RNA polymerase III subunit C2
Alternative name(s):
C128
DNA-directed RNA polymerase III 127.6 kDa polypeptide
DNA-directed RNA polymerase III subunit B
Gene namesi
Name:POLR3B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:30348. POLR3B.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • DNA-directed RNA polymerase III complex Source: MGI
  • nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Involvement in diseasei

Leukodystrophy, hypomyelinating, 8, with or without oligodontia and/or hypogonadotropic hypogonadism (HLD8)3 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionAn autosomal recessive neurodegenerative disorder characterized by early childhood onset of cerebellar ataxia and mild intellectual disabilities associated with diffuse hypomyelination apparent on brain MRI. Variable features include oligodontia and/or hypogonadotropic hypogonadism.
See also OMIM:614381
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_072344104L → F in HLD8. 1 Publication1
Natural variantiVAR_072345268S → G in HLD8. 1 Publication1
Natural variantiVAR_072346442R → C in HLD8. 1 Publication1
Natural variantiVAR_067005503T → K in HLD8. 1 PublicationCorresponds to variant rs267608683dbSNPEnsembl.1
Natural variantiVAR_067006523V → E in HLD8. 2 PublicationsCorresponds to variant rs138249161dbSNPEnsembl.1
Natural variantiVAR_072347527C → R in HLD8. 1 Publication1
Natural variantiVAR_067007620 – 652Missing in HLD8. 1 PublicationAdd BLAST33
Natural variantiVAR_067008768R → H in HLD8. 1 PublicationCorresponds to variant rs267608687dbSNPEnsembl.1
Natural variantiVAR_067009926D → E in HLD8. 1 PublicationCorresponds to variant rs267608689dbSNPEnsembl.1

Keywords - Diseasei

Disease mutation, Leukodystrophy

Organism-specific databases

DisGeNETi55703.
MalaCardsiPOLR3B.
MIMi614381. phenotype.
OpenTargetsiENSG00000013503.
Orphaneti88637. Hypomyelination - hypogonadotropic hypogonadism - hypodontia.
PharmGKBiPA134867680.

Polymorphism and mutation databases

BioMutaiPOLR3B.
DMDMi29428029.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000480921 – 1133DNA-directed RNA polymerase III subunit RPC2Add BLAST1133

Proteomic databases

EPDiQ9NW08.
MaxQBiQ9NW08.
PaxDbiQ9NW08.
PeptideAtlasiQ9NW08.
PRIDEiQ9NW08.

PTM databases

iPTMnetiQ9NW08.
PhosphoSitePlusiQ9NW08.

Expressioni

Gene expression databases

BgeeiENSG00000013503.
CleanExiHS_POLR3B.
ExpressionAtlasiQ9NW08. baseline and differential.
GenevisibleiQ9NW08. HS.

Organism-specific databases

HPAiHPA036466.

Interactioni

Subunit structurei

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.By similarity

Protein-protein interaction databases

BioGridi120828. 50 interactors.
IntActiQ9NW08. 10 interactors.
STRINGi9606.ENSP00000228347.

Structurei

3D structure databases

ProteinModelPortaliQ9NW08.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA polymerase beta chain family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1080 – 1095C4-typeAdd BLAST16

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0215. Eukaryota.
COG0085. LUCA.
GeneTreeiENSGT00860000133818.
HOGENOMiHOG000222962.
HOVERGENiHBG017744.
InParanoidiQ9NW08.
KOiK03021.
OMAiPYIIVKN.
OrthoDBiEOG091G00PZ.
PhylomeDBiQ9NW08.
TreeFamiTF103047.

Family and domain databases

CDDicd00653. RNA_pol_B_RPB2. 1 hit.
Gene3Di2.40.270.10. 2 hits.
2.40.50.150. 1 hit.
3.90.1110.10. 1 hit.
InterProiIPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007646. RNA_pol_Rpb2_4.
IPR007647. RNA_pol_Rpb2_5.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERiPTHR20856. PTHR20856. 1 hit.
PfamiPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF04566. RNA_pol_Rpb2_4. 1 hit.
PF04567. RNA_pol_Rpb2_5. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
PROSITEiPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NW08-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDVLAEEFGN LTPEQLAAPI PTVEEKWRLL PAFLKVKGLV KQHIDSFNYF
60 70 80 90 100
INVEIKKIMK ANEKVTSDAD PMWYLKYLNI YVGLPDVEES FNVTRPVSPH
110 120 130 140 150
ECRLRDMTYS APITVDIEYT RGSQRIIRNA LPIGRMPIML RSSNCVLTGK
160 170 180 190 200
TPAEFAKLNE CPLDPGGYFI VKGVEKVILI QEQLSKNRII VEADRKGAVG
210 220 230 240 250
ASVTSSTHEK KSRTNMAVKQ GRFYLRHNTL SEDIPIVIIF KAMGVESDQE
260 270 280 290 300
IVQMIGTEEH VMAAFGPSLE ECQKAQIFTQ MQALKYIGNK VRRQRMWGGG
310 320 330 340 350
PKKTKIEEAR ELLASTILTH VPVKEFNFRA KCIYTAVMVR RVILAQGDNK
360 370 380 390 400
VDDRDYYGNK RLELAGQLLS LLFEDLFKKF NSEMKKIADQ VIPKQRAAQF
410 420 430 440 450
DVVKHMRQDQ ITNGMVNAIS TGNWSLKRFK MDRQGVTQVL SRLSYISALG
460 470 480 490 500
MMTRISSQFE KTRKVSGPRS LQPSQWGMLC PSDTPEGEAC GLVKNLALMT
510 520 530 540 550
HITTDMEDGP IVKLASNLGV EDVNLLCGEE LSYPNVFLVF LNGNILGVIR
560 570 580 590 600
DHKKLVNTFR LMRRAGYINE FVSISTNLTD RCVYISSDGG RLCRPYIIVK
610 620 630 640 650
KQKPAVTNKH MEELAQGYRN FEDFLHESLV EYLDVNEEND CNIALYEHTI
660 670 680 690 700
NKDTTHLEIE PFTLLGVCAG LIPYPHHNQS PRNTYQCAMG KQAMGTIGYN
710 720 730 740 750
QRNRIDTLMY LLAYPQKPMV KTKTIELIEF EKLPAGQNAT VAVMSYSGYD
760 770 780 790 800
IEDALVLNKA SLDRGFGRCL VYKNAKCTLK RYTNQTFDKV MGPMLDAATR
810 820 830 840 850
KPIWRHEILD ADGICSPGEK VENKQVLVNK SMPTVTQIPL EGSNVPQQPQ
860 870 880 890 900
YKDVPITYKG ATDSYIEKVM ISSNAEDAFL IKMLLRQTRR PEIGDKFSSR
910 920 930 940 950
HGQKGVCGLI VPQEDMPFCD SGICPDIIMN PHGFPSRMTV GKLIELLAGK
960 970 980 990 1000
AGVLDGRFHY GTAFGGSKVK DVCEDLVRHG YNYLGKDYVT SGITGEPLEA
1010 1020 1030 1040 1050
YIYFGPVYYQ KLKHMVLDKM HARARGPRAV LTRQPTEGRS RDGGLRLGEM
1060 1070 1080 1090 1100
ERDCLIGYGA SMLLLERLMI SSDAFEVDVC GQCGLLGYSG WCHYCKSSCH
1110 1120 1130
VSSLRIPYAC KLLFQELQSM NIIPRLKLSK YNE
Length:1,133
Mass (Da):127,785
Last modified:March 28, 2003 - v2
Checksum:iF0B3AFF892DDED7D
GO
Isoform 2 (identifier: Q9NW08-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-58: Missing.

Note: No experimental confirmation available.
Show »
Length:1,075
Mass (Da):121,120
Checksum:i48188014A09A87FA
GO

Sequence cautioni

The sequence BAA91527 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAA91581 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti258E → A in AAM18214 (PubMed:12391170).Curated1
Sequence conflicti607T → I in BAG53685 (PubMed:14702039).Curated1
Sequence conflicti978R → C in BAA91527 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_072344104L → F in HLD8. 1 Publication1
Natural variantiVAR_072345268S → G in HLD8. 1 Publication1
Natural variantiVAR_072346442R → C in HLD8. 1 Publication1
Natural variantiVAR_067005503T → K in HLD8. 1 PublicationCorresponds to variant rs267608683dbSNPEnsembl.1
Natural variantiVAR_067006523V → E in HLD8. 2 PublicationsCorresponds to variant rs138249161dbSNPEnsembl.1
Natural variantiVAR_072347527C → R in HLD8. 1 Publication1
Natural variantiVAR_067007620 – 652Missing in HLD8. 1 PublicationAdd BLAST33
Natural variantiVAR_057255740T → A.Corresponds to variant rs17038460dbSNPEnsembl.1
Natural variantiVAR_067008768R → H in HLD8. 1 PublicationCorresponds to variant rs267608687dbSNPEnsembl.1
Natural variantiVAR_067009926D → E in HLD8. 1 PublicationCorresponds to variant rs267608689dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0452861 – 58Missing in isoform 2. 1 PublicationAdd BLAST58

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY092084 mRNA. Translation: AAM18214.1.
AK001161 mRNA. Translation: BAA91527.1. Different initiation.
AK001250 mRNA. Translation: BAA91581.1. Different initiation.
AK122713 mRNA. Translation: BAG53685.1.
AK291635 mRNA. Translation: BAF84324.1.
AC009721 Genomic DNA. No translation available.
AC078992 Genomic DNA. No translation available.
AC080012 Genomic DNA. No translation available.
CH471054 Genomic DNA. Translation: EAW97780.1.
BC046238 mRNA. Translation: AAH46238.1.
CCDSiCCDS53824.1. [Q9NW08-2]
CCDS9105.1. [Q9NW08-1]
RefSeqiNP_001154180.1. NM_001160708.1. [Q9NW08-2]
NP_060552.4. NM_018082.5. [Q9NW08-1]
UniGeneiHs.610795.
Hs.62696.

Genome annotation databases

EnsembliENST00000228347; ENSP00000228347; ENSG00000013503. [Q9NW08-1]
ENST00000539066; ENSP00000445721; ENSG00000013503. [Q9NW08-2]
GeneIDi55703.
KEGGihsa:55703.
UCSCiuc001tlp.3. human. [Q9NW08-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY092084 mRNA. Translation: AAM18214.1.
AK001161 mRNA. Translation: BAA91527.1. Different initiation.
AK001250 mRNA. Translation: BAA91581.1. Different initiation.
AK122713 mRNA. Translation: BAG53685.1.
AK291635 mRNA. Translation: BAF84324.1.
AC009721 Genomic DNA. No translation available.
AC078992 Genomic DNA. No translation available.
AC080012 Genomic DNA. No translation available.
CH471054 Genomic DNA. Translation: EAW97780.1.
BC046238 mRNA. Translation: AAH46238.1.
CCDSiCCDS53824.1. [Q9NW08-2]
CCDS9105.1. [Q9NW08-1]
RefSeqiNP_001154180.1. NM_001160708.1. [Q9NW08-2]
NP_060552.4. NM_018082.5. [Q9NW08-1]
UniGeneiHs.610795.
Hs.62696.

3D structure databases

ProteinModelPortaliQ9NW08.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120828. 50 interactors.
IntActiQ9NW08. 10 interactors.
STRINGi9606.ENSP00000228347.

PTM databases

iPTMnetiQ9NW08.
PhosphoSitePlusiQ9NW08.

Polymorphism and mutation databases

BioMutaiPOLR3B.
DMDMi29428029.

Proteomic databases

EPDiQ9NW08.
MaxQBiQ9NW08.
PaxDbiQ9NW08.
PeptideAtlasiQ9NW08.
PRIDEiQ9NW08.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000228347; ENSP00000228347; ENSG00000013503. [Q9NW08-1]
ENST00000539066; ENSP00000445721; ENSG00000013503. [Q9NW08-2]
GeneIDi55703.
KEGGihsa:55703.
UCSCiuc001tlp.3. human. [Q9NW08-1]

Organism-specific databases

CTDi55703.
DisGeNETi55703.
GeneCardsiPOLR3B.
GeneReviewsiPOLR3B.
H-InvDBHIX0037055.
HGNCiHGNC:30348. POLR3B.
HPAiHPA036466.
MalaCardsiPOLR3B.
MIMi614366. gene.
614381. phenotype.
neXtProtiNX_Q9NW08.
OpenTargetsiENSG00000013503.
Orphaneti88637. Hypomyelination - hypogonadotropic hypogonadism - hypodontia.
PharmGKBiPA134867680.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0215. Eukaryota.
COG0085. LUCA.
GeneTreeiENSGT00860000133818.
HOGENOMiHOG000222962.
HOVERGENiHBG017744.
InParanoidiQ9NW08.
KOiK03021.
OMAiPYIIVKN.
OrthoDBiEOG091G00PZ.
PhylomeDBiQ9NW08.
TreeFamiTF103047.

Enzyme and pathway databases

BioCyciZFISH:HS12049-MONOMER.
ReactomeiR-HSA-1834949. Cytosolic sensors of pathogen-associated DNA.
R-HSA-73780. RNA Polymerase III Chain Elongation.
R-HSA-73980. RNA Polymerase III Transcription Termination.
R-HSA-749476. RNA Polymerase III Abortive And Retractive Initiation.
R-HSA-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-HSA-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-HSA-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Miscellaneous databases

ChiTaRSiPOLR3B. human.
GenomeRNAii55703.
PROiQ9NW08.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000013503.
CleanExiHS_POLR3B.
ExpressionAtlasiQ9NW08. baseline and differential.
GenevisibleiQ9NW08. HS.

Family and domain databases

CDDicd00653. RNA_pol_B_RPB2. 1 hit.
Gene3Di2.40.270.10. 2 hits.
2.40.50.150. 1 hit.
3.90.1110.10. 1 hit.
InterProiIPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007646. RNA_pol_Rpb2_4.
IPR007647. RNA_pol_Rpb2_5.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERiPTHR20856. PTHR20856. 1 hit.
PfamiPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF04566. RNA_pol_Rpb2_4. 1 hit.
PF04567. RNA_pol_Rpb2_5. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
PROSITEiPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPC2_HUMAN
AccessioniPrimary (citable) accession number: Q9NW08
Secondary accession number(s): A8K6H0
, B3KV73, F5H1E6, Q9NW59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 28, 2003
Last modified: November 30, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.