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Protein

Adaptin ear-binding coat-associated protein 2

Gene

NECAP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in endocytosis.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Endocytosis, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Adaptin ear-binding coat-associated protein 2
Alternative name(s):
NECAP endocytosis-associated protein 2
Short name:
NECAP-2
Gene namesi
Name:NECAP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:25528. NECAP2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671268.

Polymorphism and mutation databases

BioMutaiNECAP2.
DMDMi62287168.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 263263Adaptin ear-binding coat-associated protein 2PRO_0000213071Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei181 – 1811PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9NVZ3.
MaxQBiQ9NVZ3.
PaxDbiQ9NVZ3.
PeptideAtlasiQ9NVZ3.
PRIDEiQ9NVZ3.

PTM databases

iPTMnetiQ9NVZ3.
PhosphoSiteiQ9NVZ3.

Expressioni

Gene expression databases

BgeeiQ9NVZ3.
CleanExiHS_NECAP2.
ExpressionAtlasiQ9NVZ3. baseline and differential.
GenevisibleiQ9NVZ3. HS.

Organism-specific databases

HPAiHPA028077.

Interactioni

Subunit structurei

Interacts with AP1G1 and AP2A1 components of the adapter protein complexes AP-1 and AP-2. Interacts with the GAE domain proteins GGA1, GGA2 and GGA3 (By similarity).By similarity

Protein-protein interaction databases

BioGridi120832. 31 interactions.
IntActiQ9NVZ3. 10 interactions.
MINTiMINT-1468695.
STRINGi9606.ENSP00000391942.

Structurei

3D structure databases

ProteinModelPortaliQ9NVZ3.
SMRiQ9NVZ3. Positions 3-132.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi240 – 2434WXXF motif 1
Motifi260 – 2634WXXF motif 2

Domaini

The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself (By similarity).By similarity

Sequence similaritiesi

Belongs to the NECAP family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2500. Eukaryota.
ENOG41113PS. LUCA.
GeneTreeiENSGT00390000009359.
HOGENOMiHOG000231188.
HOVERGENiHBG060621.
InParanoidiQ9NVZ3.
KOiK20069.
OMAiSGHRAND.
OrthoDBiEOG76X61J.
PhylomeDBiQ9NVZ3.
TreeFamiTF314482.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR012466. NECAP-1_N.
IPR011993. PH_dom-like.
[Graphical view]
PfamiPF07933. DUF1681. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NVZ3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEESGYESVL CVKPDVHVYR IPPRATNRGY RAAEWQLDQP SWSGRLRITA
60 70 80 90 100
KGQMAYIKLE DRTSGELFAQ APVDQFPGTA VESVTDSSRY FVIRIEDGNG
110 120 130 140 150
RRAFIGIGFG DRGDAFDFNV ALQDHFKWVK QQCEFAKQAQ NPDQGPKLDL
160 170 180 190 200
GFKEGQTIKL NIANMKKKEG AAGNPRVRPA STGGLSLLPP PPGGKTSTLI
210 220 230 240 250
PPPGEQLAVG GSLVQPAVAP SSGGAPVPWP QPNPATADIW GDFTKSTGST
260
SSQTQPGTGW VQF
Length:263
Mass (Da):28,339
Last modified:October 1, 2000 - v1
Checksum:i6F4DE1B8335852D4
GO
Isoform 2 (identifier: Q9NVZ3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     223-263: GGAPVPWPQP...TQPGTGWVQF → DQLPARPSQA...ALLSWPVFGA

Show »
Length:273
Mass (Da):29,464
Checksum:i463D31DEFCA0102E
GO
Isoform 3 (identifier: Q9NVZ3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     164-172: NMKKKEGAA → VSSTLAWLW
     173-263: Missing.

Note: No experimental confirmation available.
Show »
Length:172
Mass (Da):19,419
Checksum:i0EBC5856B326788A
GO
Isoform 4 (identifier: Q9NVZ3-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     6-31: Missing.

Show »
Length:237
Mass (Da):25,267
Checksum:i8F5B5B501EEE8E8E
GO

Sequence cautioni

The sequence BAB15758.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti192 – 1921P → S in BAG63581 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti149 – 1491D → A.
Corresponds to variant rs35056694 [ dbSNP | Ensembl ].
VAR_034154

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei6 – 3126Missing in isoform 4. 1 PublicationVSP_041726Add
BLAST
Alternative sequencei164 – 1729NMKKKEGAA → VSSTLAWLW in isoform 3. 1 PublicationVSP_013234
Alternative sequencei173 – 26391Missing in isoform 3. 1 PublicationVSP_013235Add
BLAST
Alternative sequencei223 – 26341GGAPV…GWVQF → DQLPARPSQAQAGSSSDLST VFPHVTSGKALPHLGQRKED EALLSWPVFGA in isoform 2. 1 PublicationVSP_013236Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK024468 mRNA. Translation: BAB15758.1. Different initiation.
AK302227 mRNA. Translation: BAG63581.1.
AK001282 mRNA. Translation: BAA91598.1.
AK023545 mRNA. Translation: BAB14605.1.
AL669962, AL137802 Genomic DNA. Translation: CAH71454.1.
AL137802, AL669962 Genomic DNA. Translation: CAI22843.1.
BC017014 mRNA. Translation: AAH17014.1.
BC018914 mRNA. Translation: AAH18914.1.
CCDSiCCDS173.1. [Q9NVZ3-1]
CCDS44066.1. [Q9NVZ3-2]
CCDS44067.1. [Q9NVZ3-4]
RefSeqiNP_001138749.1. NM_001145277.1. [Q9NVZ3-2]
NP_001138750.1. NM_001145278.1. [Q9NVZ3-4]
NP_060560.1. NM_018090.4. [Q9NVZ3-1]
UniGeneiHs.437385.

Genome annotation databases

EnsembliENST00000337132; ENSP00000338746; ENSG00000157191. [Q9NVZ3-1]
ENST00000443980; ENSP00000391942; ENSG00000157191. [Q9NVZ3-2]
ENST00000457722; ENSP00000407091; ENSG00000157191. [Q9NVZ3-4]
ENST00000492095; ENSP00000427620; ENSG00000157191. [Q9NVZ3-1]
GeneIDi55707.
KEGGihsa:55707.
UCSCiuc001ayo.4. human. [Q9NVZ3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK024468 mRNA. Translation: BAB15758.1. Different initiation.
AK302227 mRNA. Translation: BAG63581.1.
AK001282 mRNA. Translation: BAA91598.1.
AK023545 mRNA. Translation: BAB14605.1.
AL669962, AL137802 Genomic DNA. Translation: CAH71454.1.
AL137802, AL669962 Genomic DNA. Translation: CAI22843.1.
BC017014 mRNA. Translation: AAH17014.1.
BC018914 mRNA. Translation: AAH18914.1.
CCDSiCCDS173.1. [Q9NVZ3-1]
CCDS44066.1. [Q9NVZ3-2]
CCDS44067.1. [Q9NVZ3-4]
RefSeqiNP_001138749.1. NM_001145277.1. [Q9NVZ3-2]
NP_001138750.1. NM_001145278.1. [Q9NVZ3-4]
NP_060560.1. NM_018090.4. [Q9NVZ3-1]
UniGeneiHs.437385.

3D structure databases

ProteinModelPortaliQ9NVZ3.
SMRiQ9NVZ3. Positions 3-132.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120832. 31 interactions.
IntActiQ9NVZ3. 10 interactions.
MINTiMINT-1468695.
STRINGi9606.ENSP00000391942.

PTM databases

iPTMnetiQ9NVZ3.
PhosphoSiteiQ9NVZ3.

Polymorphism and mutation databases

BioMutaiNECAP2.
DMDMi62287168.

Proteomic databases

EPDiQ9NVZ3.
MaxQBiQ9NVZ3.
PaxDbiQ9NVZ3.
PeptideAtlasiQ9NVZ3.
PRIDEiQ9NVZ3.

Protocols and materials databases

DNASUi55707.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000337132; ENSP00000338746; ENSG00000157191. [Q9NVZ3-1]
ENST00000443980; ENSP00000391942; ENSG00000157191. [Q9NVZ3-2]
ENST00000457722; ENSP00000407091; ENSG00000157191. [Q9NVZ3-4]
ENST00000492095; ENSP00000427620; ENSG00000157191. [Q9NVZ3-1]
GeneIDi55707.
KEGGihsa:55707.
UCSCiuc001ayo.4. human. [Q9NVZ3-1]

Organism-specific databases

CTDi55707.
GeneCardsiNECAP2.
HGNCiHGNC:25528. NECAP2.
HPAiHPA028077.
MIMi611624. gene.
neXtProtiNX_Q9NVZ3.
PharmGKBiPA142671268.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2500. Eukaryota.
ENOG41113PS. LUCA.
GeneTreeiENSGT00390000009359.
HOGENOMiHOG000231188.
HOVERGENiHBG060621.
InParanoidiQ9NVZ3.
KOiK20069.
OMAiSGHRAND.
OrthoDBiEOG76X61J.
PhylomeDBiQ9NVZ3.
TreeFamiTF314482.

Miscellaneous databases

ChiTaRSiNECAP2. human.
GeneWikiiNECAP2.
GenomeRNAii55707.
PROiQ9NVZ3.
SOURCEiSearch...

Gene expression databases

BgeeiQ9NVZ3.
CleanExiHS_NECAP2.
ExpressionAtlasiQ9NVZ3. baseline and differential.
GenevisibleiQ9NVZ3. HS.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR012466. NECAP-1_N.
IPR011993. PH_dom-like.
[Graphical view]
PfamiPF07933. DUF1681. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Characterization of long cDNA clones from human adult spleen."
    Hattori A., Okumura K., Nagase T., Kikuno R., Hirosawa M., Ohara O.
    DNA Res. 7:357-366(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Spleen.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 4).
    Tissue: Placenta, Spleen and Testis.
  3. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Colon and Eye.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Toward a comprehensive characterization of a human cancer cell phosphoproteome."
    Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J., Mohammed S.
    J. Proteome Res. 12:260-271(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-181, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma and Erythroleukemia.
  8. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNECP2_HUMAN
AccessioniPrimary (citable) accession number: Q9NVZ3
Secondary accession number(s): B4DY19
, E9PGQ8, Q5VSU4, Q5VSU5, Q9H7L1, Q9H8L1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: October 1, 2000
Last modified: July 6, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.