Q9NVZ3 (NECP2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adaptin ear-binding coat-associated protein 2

Alternative name(s):
NECAP endocytosis-associated protein 2
Short name=NECAP-2

Gene names

Name:

NECAP2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in endocytosis By similarity.
Subunit structure	Interacts with AP1G1 and AP2A1 components of the adapter protein complexes AP-1 and AP-2. Interacts with the GAE domain proteins GGA1, GGA2 and GGA3 By similarity.
Subcellular location	Cytoplasmic vesicle › clathrin-coated vesicle membrane By similarity. Cell membrane By similarity. Note: Colocalizes with AP-2 at the plasma membrane By similarity.
Domain	The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself By similarity.
Sequence similarities	Belongs to the NECAP family.
Sequence caution	The sequence BAB15758.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Endocytosis Protein transport Transport
Cellular component	Cell membrane Cytoplasmic vesicle Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endocytosis Inferred from sequence or structural similarity. Source: UniProtKB protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	clathrin vesicle coat Inferred from sequence or structural similarity. Source: UniProtKB coated pit Inferred from sequence or structural similarity. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NVZ3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NVZ3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     223-263: GGAPVPWPQP...TQPGTGWVQF → DQLPARPSQA...ALLSWPVFGA

Isoform 3 (identifier: Q9NVZ3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     164-172: NMKKKEGAA → VSSTLAWLW
     173-263: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q9NVZ3-4)

The sequence of this isoform differs from the canonical sequence as follows:
     6-31: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	Adaptin ear-binding coat-associated protein 2		PRO_0000213071
Regions
	Motif	240 – 243	4	WXXF motif 1
	Motif	260 – 263	4	WXXF motif 2
Natural variations
	Alternative sequence	6 – 31	26	Missing in isoform 4.		VSP_041726
	Alternative sequence	164 – 172	9	NMKKKEGAA → VSSTLAWLW in isoform 3.		VSP_013234
	Alternative sequence	173 – 263	91	Missing in isoform 3.		VSP_013235
	Alternative sequence	223 – 263	41	GGAPV…GWVQF → DQLPARPSQAQAGSSSDLST VFPHVTSGKALPHLGQRKED EALLSWPVFGA in isoform 2.		VSP_013236
	Natural variant	149	1	D → A. Corresponds to variant rs35056694 [ dbSNP | Ensembl ].		VAR_034154
Experimental info
	Sequence conflict	192	1	P → S in BAG63581. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 6F4DE1B8335852D4	FASTA	263	28,339
	10 20 30 40 50 60 MEESGYESVL CVKPDVHVYR IPPRATNRGY RAAEWQLDQP SWSGRLRITA KGQMAYIKLE 70 80 90 100 110 120 DRTSGELFAQ APVDQFPGTA VESVTDSSRY FVIRIEDGNG RRAFIGIGFG DRGDAFDFNV 130 140 150 160 170 180 ALQDHFKWVK QQCEFAKQAQ NPDQGPKLDL GFKEGQTIKL NIANMKKKEG AAGNPRVRPA 190 200 210 220 230 240 STGGLSLLPP PPGGKTSTLI PPPGEQLAVG GSLVQPAVAP SSGGAPVPWP QPNPATADIW 250 260 GDFTKSTGST SSQTQPGTGW VQF
	Isoform 2 [UniParc]. Checksum: 463D31DEFCA0102E	FASTA	273	29,464
	10 20 30 40 50 60 MEESGYESVL CVKPDVHVYR IPPRATNRGY RAAEWQLDQP SWSGRLRITA KGQMAYIKLE 70 80 90 100 110 120 DRTSGELFAQ APVDQFPGTA VESVTDSSRY FVIRIEDGNG RRAFIGIGFG DRGDAFDFNV 130 140 150 160 170 180 ALQDHFKWVK QQCEFAKQAQ NPDQGPKLDL GFKEGQTIKL NIANMKKKEG AAGNPRVRPA 190 200 210 220 230 240 STGGLSLLPP PPGGKTSTLI PPPGEQLAVG GSLVQPAVAP SSDQLPARPS QAQAGSSSDL 250 260 270 STVFPHVTSG KALPHLGQRK EDEALLSWPV FGA
	Isoform 3 [UniParc]. Checksum: 0EBC5856B326788A	FASTA	172	19,419
	10 20 30 40 50 60 MEESGYESVL CVKPDVHVYR IPPRATNRGY RAAEWQLDQP SWSGRLRITA KGQMAYIKLE 70 80 90 100 110 120 DRTSGELFAQ APVDQFPGTA VESVTDSSRY FVIRIEDGNG RRAFIGIGFG DRGDAFDFNV 130 140 150 160 170 ALQDHFKWVK QQCEFAKQAQ NPDQGPKLDL GFKEGQTIKL NIAVSSTLAW LW
	Isoform 4 [UniParc]. Checksum: 8F5B5B501EEE8E8E	FASTA	237	25,267
	10 20 30 40 50 60 MEESGAAEWQ LDQPSWSGRL RITAKGQMAY IKLEDRTSGE LFAQAPVDQF PGTAVESVTD 70 80 90 100 110 120 SSRYFVIRIE DGNGRRAFIG IGFGDRGDAF DFNVALQDHF KWVKQQCEFA KQAQNPDQGP 130 140 150 160 170 180 KLDLGFKEGQ TIKLNIANMK KKEGAAGNPR VRPASTGGLS LLPPPPGGKT STLIPPPGEQ 190 200 210 220 230 LAVGGSLVQP AVAPSSGGAP VPWPQPNPAT ADIWGDFTKS TGSTSSQTQP GTGWVQF

References

[1]	"Characterization of long cDNA clones from human adult spleen." Hattori A., Okumura K., Nagase T., Kikuno R., Hirosawa M., Ohara O. DNA Res. 7:357-366(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Spleen.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 4). Tissue: Placenta, Spleen and Testis.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Colon and Eye.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK024468 mRNA. Translation: BAB15758.1. Different initiation. AK302227 mRNA. Translation: BAG63581.1. AK001282 mRNA. Translation: BAA91598.1. AK023545 mRNA. Translation: BAB14605.1. AL669962, AL137802 Genomic DNA. Translation: CAH71454.1. AL137802, AL669962 Genomic DNA. Translation: CAI22843.1. BC017014 mRNA. Translation: AAH17014.1. BC018914 mRNA. Translation: AAH18914.1.
IPI	IPI00018188. IPI00073436. IPI00922268. IPI00967036.
RefSeq	NP_001138749.1. NM_001145277.1. NP_001138750.1. NM_001145278.1. NP_060560.1. NM_018090.4.
UniGene	Hs.437385.
3D structure databases
ProteinModelPortal	Q9NVZ3.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NVZ3. 6 interactions.
MINT	MINT-1468695.
STRING	9606.ENSP00000263498.
PTM databases
PhosphoSite	Q9NVZ3.
Polymorphism databases
DMDM	62287168.
Proteomic databases
PaxDb	Q9NVZ3.
PRIDE	Q9NVZ3.
Protocols and materials databases
DNASU	55707.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000337132; ENSP00000338746; ENSG00000157191. ENST00000406746; ENSP00000383925; ENSG00000157191. ENST00000443980; ENSP00000391942; ENSG00000157191. ENST00000457722; ENSP00000407091; ENSG00000157191. ENST00000492095; ENSP00000427620; ENSG00000157191.
GeneID	55707.
KEGG	hsa:55707.
UCSC	uc001ayo.3. human. uc001ayq.3. human. uc010ocd.2. human.
Organism-specific databases
CTD	55707.
GeneCards	GC01P016767.
HGNC	HGNC:25528. NECAP2.
HPA	HPA028077.
MIM	611624. gene.
neXtProt	NX_Q9NVZ3.
PharmGKB	PA142671268.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG329551.
HOGENOM	HOG000231188.
HOVERGEN	HBG060621.
OMA	WPQPKPA.
OrthoDB	EOG4M399M.
Gene expression databases
ArrayExpress	Q9NVZ3.
Bgee	Q9NVZ3.
CleanEx	HS_NECAP2.
Genevestigator	Q9NVZ3.
GermOnline	ENSG00000157191. Homo sapiens.
Family and domain databases
Gene3D	2.30.29.30. 1 hit.
InterPro	IPR012466. NECAP-1. IPR011993. PH_like_dom. [Graphical view]
Pfam	PF07933. DUF1681. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	55707.
NextBio	60567.
SOURCE	Search...

Entry information

Entry name

NECP2_HUMAN

Accession

Primary (citable) accession number: Q9NVZ3
Secondary accession number(s): B4DY19 , E9PGQ8, Q5VSU4, Q5VSU5, Q9H7L1, Q9H8L1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.