Q9NVU7 (SDA1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein SDA1 homolog

Alternative name(s):
Nucleolar protein 130
SDA1 domain-containing protein 1
Short name=hSDA

Gene names

Name:	SDAD1
Synonyms:	NUC130

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	687 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for 60S pre-ribosomal subunits export to the cytoplasm By similarity.
Subcellular location	Nucleus › nucleolus Ref.5 Ref.6.
Tissue specificity	Highly expressed in testis, kidney, spleen, brain and fetal tissues. Also expressed at lower level in heart, lung, liver, small intestine, ovary, uterus, mammary gland and placenta. Ref.6 Ref.7
Polymorphism	Variations in SDAD1 may be a cause of susceptibility to seasonal allergic rhinitis (SAR). SAR is a common allergic disorder characterized by episodes of sneezing, rhinorrhea, and swelling of the nasal mucosa.
Miscellaneous	DAZL and PUM2 bind its 3'-UTR mRNA, suggesting that these proteins may regulate its translation.
Sequence similarities	Belongs to the SDA1 family.
Sequence caution	The sequence AAI07896.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence BAA91648.1 differs from that shown. Reason: Frameshift at position 53. The sequence BAB14177.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAB14790.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Protein transport Ribosome biogenesis Transport
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin cytoskeleton organization Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW ribosomal large subunit biogenesis Inferred from sequence or structural similarity. Source: UniProtKB ribosomal large subunit export from nucleus Inferred from electronic annotation. Source: InterPro
Cellular_component	nucleolus Inferred from direct assay Ref.5. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TGIF1	Q15583	1	EBI-1043768,EBI-714215

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NVU7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NVU7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-97: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 687	687	Protein SDA1 homolog		PRO_0000287482
Regions
	Coiled coil	253 – 315	63	Potential
Amino acid modifications
	Modified residue	232	1	Phosphoserine Ref.12 Ref.15
	Modified residue	234	1	Phosphoserine Ref.12 Ref.15
	Modified residue	236	1	Phosphoserine Ref.12 Ref.15
	Modified residue	585	1	Phosphoserine Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.15
	Modified residue	595	1	Phosphoserine Ref.15
Natural variations
	Alternative sequence	1 – 97	97	Missing in isoform 2.		VSP_025505
	Natural variant	258	1	K → Q. Corresponds to variant rs15481 [ dbSNP | Ensembl ].		VAR_032312
	Natural variant	490	1	A → D. Corresponds to variant rs34627298 [ dbSNP | Ensembl ].		VAR_032313
	Natural variant	575	1	S → C. Ref.1 Ref.4 Corresponds to variant rs2242471 [ dbSNP | Ensembl ].		VAR_032314
	Natural variant	660	1	V → I. Corresponds to variant rs17001276 [ dbSNP | Ensembl ].		VAR_032315
Experimental info
	Sequence conflict	186	1	E → G in CAH18368. Ref.1
	Sequence conflict	288	1	H → L in BAA91648. Ref.4
	Sequence conflict	319	1	N → S in CAH18368. Ref.1
	Sequence conflict	396	1	N → S in CAH18368. Ref.1
	Sequence conflict	617	1	A → S in BAB14177. Ref.4
	Sequence conflict	634	1	T → A in CAH18368. Ref.1
	Sequence conflict	651	1	F → L in CAH18368. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 18, 2010. Version 3. Checksum: B01EE09383EA959C	FASTA	687	79,871
	10 20 30 40 50 60 MSNRNNNKLP SNLPQLQNLI KRDPPAYIEE FLQQYNHYKS NVEIFKLQPN KPSKELAELV 70 80 90 100 110 120 MFMAQISHCY PEYLSNFPQE VKDLLSCNHT VLDPDLRMTF CKALILLRNK NLINPSSLLE 130 140 150 160 170 180 LFFELFRCHD KLLRKTLYTH IVTDIKNINA KHKNNKVNVV LQNFMYTMLR DSNATAAKMS 190 200 210 220 230 240 LDVMIELYRR NIWNDAKTVN VITTACFSKV TKILVAALTF FLGKDEDEKQ DSDSESEDDG 250 260 270 280 290 300 PTARDLLVQY ATGKKSSKNK KKLEKAMKVL KKQKKKKKPE VFNFSAIHLI HDPQDFAEKL 310 320 330 340 350 360 LKQLECCKER FEVKMMLMNL ISRLVGIHEL FLFNFYPFLQ RFLQPHQREV TKILLFAAQA 370 380 390 400 410 420 SHHLVPPEII QSLLMTVANN FVTDKNSGEV MTVGINAIKE ITARCPLAMT EELLQDLAQY 430 440 450 460 470 480 KTHKDKNVMM SARTLIHLFR TLNPQMLQKK FRGKPTEASI EARVQEYGEL DAKDYIPGAE 490 500 510 520 530 540 VLEVEKEENA ENDEDGWEST SLSEEEDADG EWIDVQHSSD EEQQEISKKL NSMPMEERKA 550 560 570 580 590 600 KAAAISTSRV LTQEDFQKIR MAQMRKELDA APGKSQKRKY IEIDSDEEPR GELLSLRDIE 610 620 630 640 650 660 RLHKKPKSDK ETRLATAMAG KTDRKEFVRK KTKTNPFSSS TNKEKKKQKN FMMMRYSQNV 670 680 RSKNKRSFRE KQLALRDALL KKRKRMK
	Isoform 2 [UniParc]. Checksum: 06780B225C43F041	FASTA	590	68,455
	10 20 30 40 50 60 MTFCKALILL RNKNLINPSS LLELFFELFR CHDKLLRKTL YTHIVTDIKN INAKHKNNKV 70 80 90 100 110 120 NVVLQNFMYT MLRDSNATAA KMSLDVMIEL YRRNIWNDAK TVNVITTACF SKVTKILVAA 130 140 150 160 170 180 LTFFLGKDED EKQDSDSESE DDGPTARDLL VQYATGKKSS KNKKKLEKAM KVLKKQKKKK 190 200 210 220 230 240 KPEVFNFSAI HLIHDPQDFA EKLLKQLECC KERFEVKMML MNLISRLVGI HELFLFNFYP 250 260 270 280 290 300 FLQRFLQPHQ REVTKILLFA AQASHHLVPP EIIQSLLMTV ANNFVTDKNS GEVMTVGINA 310 320 330 340 350 360 IKEITARCPL AMTEELLQDL AQYKTHKDKN VMMSARTLIH LFRTLNPQML QKKFRGKPTE 370 380 390 400 410 420 ASIEARVQEY GELDAKDYIP GAEVLEVEKE ENAENDEDGW ESTSLSEEED ADGEWIDVQH 430 440 450 460 470 480 SSDEEQQEIS KKLNSMPMEE RKAKAAAIST SRVLTQEDFQ KIRMAQMRKE LDAAPGKSQK 490 500 510 520 530 540 RKYIEIDSDE EPRGELLSLR DIERLHKKPK SDKETRLATA MAGKTDRKEF VRKKTKTNPF 550 560 570 580 590 SSSTNKEKKK QKNFMMMRYS QNVRSKNKRS FREKQLALRD ALLKKRKRMK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT CYS-575. Tissue: Rectum tumor.
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-277 (ISOFORM 1). Tissue: PNS and Uterus.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 50-687 (ISOFORM 1), VARIANT CYS-575.
[5]	"Directed proteomic analysis of the human nucleolus." Andersen J.S., Lyon C.E., Fox A.H., Leung A.K.L., Lam Y.W., Steen H., Mann M., Lamond A.I. Curr. Biol. 12:1-11(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Expression and localization studies of hSDA, the human ortholog of the yeast SDA1 gene." Babbio F., Farinacci M., Saracino F., Carbone M.L., Privitera E. Cell Cycle 3:486-490(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[7]	"Identification and characterization of RNA sequences to which human PUMILIO-2 (PUM2) and deleted in Azoospermia-like (DAZL) bind." Fox M., Urano J., Reijo Pera R.A. Genomics 85:92-105(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[8]	"Association of a haplotype block spanning SDAD1 gene and CXC chemokine genes with allergic rhinitis." Zhang J., Noguchi E., Migita O., Yokouchi Y., Nakayama J., Shibasaki M., Arinami T. J. Allergy Clin. Immunol. 115:548-554(2005) [PubMed] [Europe PMC] [Abstract] Cited for: POLYMORPHISM.
[9]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-585, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-585, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-585, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232; SER-234; SER-236 AND SER-585, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[13]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-585, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[14]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232; SER-234; SER-236; SER-585 AND SER-595, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR749574 mRNA. Translation: CAH18368.1. AC110615 Genomic DNA. No translation available. AC112719 Genomic DNA. No translation available. AC115628 Genomic DNA. No translation available. BC054040 mRNA. Translation: AAH54040.1. BC063797 mRNA. Translation: AAH63797.1. BC107895 mRNA. Translation: AAI07896.1. Sequence problems. AK001360 mRNA. Translation: BAA91648.1. Frameshift. AK022683 mRNA. Translation: BAB14177.1. Different initiation. AK024031 mRNA. Translation: BAB14790.1. Different initiation.
IPI	IPI00018240. IPI00797421.
RefSeq	NP_060585.2. NM_018115.2.
UniGene	Hs.632604.
3D structure databases
ProteinModelPortal	Q9NVU7.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NVU7. 3 interactions.
STRING	9606.ENSP00000348596.
PTM databases
PhosphoSite	Q9NVU7.
Polymorphism databases
DMDM	296452964.
Proteomic databases
PaxDb	Q9NVU7.
PeptideAtlas	Q9NVU7.
PRIDE	Q9NVU7.
Protocols and materials databases
DNASU	55153.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000356260; ENSP00000348596; ENSG00000198301.
GeneID	55153.
KEGG	hsa:55153.
UCSC	uc003hje.4. human.
Organism-specific databases
CTD	55153.
GeneCards	GC04M076871.
H-InvDB	HIX0025559.
HGNC	HGNC:25537. SDAD1.
HPA	HPA035949.
neXtProt	NX_Q9NVU7.
PharmGKB	PA134961441.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG292808.
HOGENOM	HOG000204601.
HOVERGEN	HBG059552.
InParanoid	Q9NVU7.
KO	K14856.
OMA	RDPPAYV.
OrthoDB	EOG412M53.
Gene expression databases
ArrayExpress	Q9NVU7.
Bgee	Q9NVU7.
CleanEx	HS_SDAD1.
Genevestigator	Q9NVU7.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. IPR027312. Sda1. IPR007949. SDA1_dom. IPR012977. Uncharacterised_NUC130/133_N. [Graphical view]
PANTHER	PTHR12730:SF0. PTHR12730:SF0. 1 hit.
Pfam	PF08158. NUC130_3NT. 1 hit. PF05285. SDA1. 2 hits. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	55153.
NextBio	58895.

Entry information

Entry name

SDA1_HUMAN

Accession

Primary (citable) accession number: Q9NVU7
Secondary accession number(s): Q32Q11 , Q68D52, Q7Z5U4, Q9H831, Q9H9P6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 82 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.