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Protein

DNA-directed RNA polymerase III subunit RPC5

Gene

POLR3E

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAntiviral defense, Immunity, Innate immunity, Transcription

Enzyme and pathway databases

ReactomeiR-HSA-1834949 Cytosolic sensors of pathogen-associated DNA
R-HSA-73780 RNA Polymerase III Chain Elongation
R-HSA-73980 RNA Polymerase III Transcription Termination
R-HSA-749476 RNA Polymerase III Abortive And Retractive Initiation
R-HSA-76061 RNA Polymerase III Transcription Initiation From Type 1 Promoter
R-HSA-76066 RNA Polymerase III Transcription Initiation From Type 2 Promoter
R-HSA-76071 RNA Polymerase III Transcription Initiation From Type 3 Promoter

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase III subunit RPC5
Short name:
RNA polymerase III subunit C5
Alternative name(s):
DNA-directed RNA polymerase III 80 kDa polypeptide
Gene namesi
Name:POLR3E
Synonyms:KIAA1452
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000058600.15
HGNCiHGNC:30347 POLR3E
MIMi617815 gene
neXtProtiNX_Q9NVU0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi101060521
55718
OpenTargetsiENSG00000058600
PharmGKBiPA134964025

Polymorphism and mutation databases

BioMutaiPOLR3E
DMDMi29428028

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000739701 – 708DNA-directed RNA polymerase III subunit RPC5Add BLAST708

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei161PhosphoserineCombined sources1
Modified residuei162PhosphoserineCombined sources1
Cross-linki171Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei192PhosphoserineCombined sources1
Modified residuei224PhosphotyrosineCombined sources1
Cross-linki432Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki498Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources
Cross-linki498Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei503PhosphoserineCombined sources1
Modified residuei522PhosphoserineCombined sources1
Cross-linki659Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9NVU0
MaxQBiQ9NVU0
PaxDbiQ9NVU0
PeptideAtlasiQ9NVU0
PRIDEiQ9NVU0
ProteomicsDBi82857
82858 [Q9NVU0-2]
82859 [Q9NVU0-3]

PTM databases

iPTMnetiQ9NVU0
PhosphoSitePlusiQ9NVU0

Expressioni

Gene expression databases

BgeeiENSG00000058600
CleanExiHS_POLR3E
ExpressionAtlasiQ9NVU0 baseline and differential
GenevisibleiQ9NVU0 HS

Organism-specific databases

HPAiHPA041419
HPA041477

Interactioni

Subunit structurei

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). Interacts with POLR3D/RPC4.By similarity1 Publication

Protein-protein interaction databases

BioGridi120840, 54 interactors
3190820, 9 interactors
IntActiQ9NVU0, 39 interactors
MINTiQ9NVU0
STRINGi9606.ENSP00000299853

Structurei

3D structure databases

ProteinModelPortaliQ9NVU0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiKOG2354 Eukaryota
ENOG410Y1X8 LUCA
GeneTreeiENSGT00390000016123
HOGENOMiHOG000069875
HOVERGENiHBG039706
InParanoidiQ9NVU0
KOiK14721
OMAiRNMIQSR
OrthoDBiEOG091G0BTS
PhylomeDBiQ9NVU0
TreeFamiTF103050

Family and domain databases

InterProiView protein in InterPro
IPR006886 RNA_pol_III_Rpc5
PfamiView protein in Pfam
PF04801 Sin_N, 1 hit

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NVU0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MANEEDDPVV QEIDVYLAKS LAEKLYLFQY PVRPASMTYD DIPHLSAKIK
60 70 80 90 100
PKQQKVELEM AIDTLNPNYC RSKGEQIALN VDGACADETS TYSSKLMDKQ
110 120 130 140 150
TFCSSQTTSN TSRYAAALYR QGELHLTPLH GILQLRPSFS YLDKADAKHR
160 170 180 190 200
EREAANEAGD SSQDEAEDDV KQITVRFSRP ESEQARQRRV QSYEFLQKKH
210 220 230 240 250
AEEPWVHLHY YGLRDSRSEH ERQYLLCPGS SGVENTELVK SPSEYLMMLM
260 270 280 290 300
PPSQEEEKDK PVAPSNVLSM AQLRTLPLAD QIKILMKNVK VMPFANLMSL
310 320 330 340 350
LGPSIDSVAV LRGIQKVAML VQGNWVVKSD ILYPKDSSSP HSGVPAEVLC
360 370 380 390 400
RGRDFVMWKF TQSRWVVRKE VATVTKLCAE DVKDFLEHMA VVRINKGWEF
410 420 430 440 450
ILPYDGEFIK KHPDVVQRQH MLWTGIQAKL EKVYNLVKET MPKKPDAQSG
460 470 480 490 500
PAGLVCGDQR IQVAKTKAQQ NHALLERELQ RRKEQLRVPA VPPGVRIKEE
510 520 530 540 550
PVSEEGEEDE EQEAEEEPMD TSPSGLHSKL ANGLPLGRAA GTDSFNGHPP
560 570 580 590 600
QGCASTPVAR ELKAFVEATF QRQFVLTLSE LKRLFNLHLA SLPPGHTLFS
610 620 630 640 650
GISDRMLQDT VLAAGCKQIL VPFPPQTAAS PDEQKVFALW ESGDMSDQHR
660 670 680 690 700
QVLLEIFSKN YRVRRNMIQS RLTQECGEDL SKQEVDKVLK DCCVSYGGMW

YLKGTVQS
Length:708
Mass (Da):79,898
Last modified:October 1, 2000 - v1
Checksum:iE9DB71615CC367A5
GO
Isoform 2 (identifier: Q9NVU0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     649-690: Missing.

Show »
Length:666
Mass (Da):74,827
Checksum:iA4E140C8BB0288F4
GO
Isoform 3 (identifier: Q9NVU0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     623-708: FPPQTAASPD...MWYLKGTVQS → VSRALPARGMGGGGWGEPAVGRPRGDTGGHAW

Note: No experimental confirmation available. May result from the retention of an intron in the cDNA.
Show »
Length:654
Mass (Da):73,139
Checksum:iCE65F08FBD429AE7
GO
Isoform 4 (identifier: Q9NVU0-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     648-668: Missing.

Note: No experimental confirmation available.
Show »
Length:687
Mass (Da):77,215
Checksum:i0E1AEF649E9F5B70
GO
Isoform 5 (identifier: Q9NVU0-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     13-48: Missing.

Note: No experimental confirmation available.
Show »
Length:672
Mass (Da):75,758
Checksum:i3B8AC9C189AF29DB
GO

Sequence cautioni

The sequence BAA95976 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB14481 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14D → N in BAB14437 (PubMed:10819331).Curated1
Sequence conflicti80N → D in BAG62408 (PubMed:14702039).Curated1
Sequence conflicti148K → E in BAB14437 (PubMed:10819331).Curated1
Sequence conflicti316K → Q in AAM18215 (PubMed:12391170).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02462346S → A. Corresponds to variant dbSNP:rs2347Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04637413 – 48Missing in isoform 5. 1 PublicationAdd BLAST36
Alternative sequenceiVSP_007064623 – 708FPPQT…GTVQS → VSRALPARGMGGGGWGEPAV GRPRGDTGGHAW in isoform 3. 1 PublicationAdd BLAST86
Alternative sequenceiVSP_044710648 – 668Missing in isoform 4. 1 PublicationAdd BLAST21
Alternative sequenceiVSP_007065649 – 690Missing in isoform 2. 2 PublicationsAdd BLAST42

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY092085 mRNA Translation: AAM18215.1
AB040885 mRNA Translation: BAA95976.1 Different initiation.
AK001371 mRNA Translation: BAA91655.1
AK023160 mRNA Translation: BAB14437.1
AK023231 mRNA Translation: BAB14481.1 Different initiation.
AK296813 mRNA Translation: BAG59386.1
AK300735 mRNA Translation: BAG62408.1
AC009034 Genomic DNA No translation available.
AC092338 Genomic DNA No translation available.
BC000285 mRNA Translation: AAH00285.1
CCDSiCCDS10605.1 [Q9NVU0-1]
CCDS58432.1 [Q9NVU0-4]
CCDS58433.1 [Q9NVU0-2]
CCDS58434.1 [Q9NVU0-5]
RefSeqiNP_001244962.1, NM_001258033.1 [Q9NVU0-4]
NP_001244963.1, NM_001258034.1 [Q9NVU0-5]
NP_001244964.1, NM_001258035.1
NP_001244965.1, NM_001258036.1 [Q9NVU0-2]
NP_060589.1, NM_018119.3 [Q9NVU0-1]
XP_006721126.1, XM_006721063.2 [Q9NVU0-1]
XP_006726676.1, XM_006726613.1 [Q9NVU0-1]
UniGeneiHs.460298

Genome annotation databases

EnsembliENST00000299853; ENSP00000299853; ENSG00000058600 [Q9NVU0-1]
ENST00000359210; ENSP00000352140; ENSG00000058600 [Q9NVU0-2]
ENST00000418581; ENSP00000399254; ENSG00000058600 [Q9NVU0-5]
ENST00000564209; ENSP00000456967; ENSG00000058600 [Q9NVU0-4]
ENST00000638476; ENSP00000492534; ENSG00000284282 [Q9NVU0-5]
ENST00000638525; ENSP00000492639; ENSG00000284282 [Q9NVU0-2]
ENST00000639149; ENSP00000492586; ENSG00000284282 [Q9NVU0-4]
ENST00000640588; ENSP00000492263; ENSG00000284282 [Q9NVU0-1]
GeneIDi55718
KEGGihsa:101060521
hsa:55718
UCSCiuc002dkk.5 human [Q9NVU0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiRPC5_HUMAN
AccessioniPrimary (citable) accession number: Q9NVU0
Secondary accession number(s): B4DL24
, B4DUP6, H3BT11, Q9BWF7, Q9H8W8, Q9H907, Q9P276
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: October 1, 2000
Last modified: June 20, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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