ID   INT10_HUMAN             Reviewed;         710 AA.
AC   Q9NVR2; Q6IA93; Q7L538; Q7L8C8; Q9H3W8;
DT   16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2006, sequence version 2.
DT   25-JAN-2012, entry version 81.
DE   RecName: Full=Integrator complex subunit 10;
DE            Short=Int10;
GN   Name=INTS10; Synonyms=C8orf35;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Teratocarcinoma;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 74-710.
RC   TISSUE=Lung, and Neuroblastoma;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 114-710.
RA   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT   "Cloning of human full open reading frames in Gateway(TM) system entry
RT   vector (pDONR201).";
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 377-710.
RC   TISSUE=Lymph node;
RX   PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA   Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H.,
RA   Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K.,
RA   Ottenwaelder B., Poustka A., Wiemann S., Schupp I.;
RT   "The full-ORF clone resource of the German cDNA consortium.";
RL   BMC Genomics 8:399-399(2007).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE INTEGRATOR
RP   COMPLEX, AND SUBCELLULAR LOCATION.
RX   PubMed=16239144; DOI=10.1016/j.cell.2005.08.019;
RA   Baillat D., Hakimi M.-A., Naeaer A.M., Shilatifard A., Cooch N.,
RA   Shiekhattar R.;
RT   "Integrator, a multiprotein mediator of small nuclear RNA processing,
RT   associates with the C-terminal repeat of RNA polymerase II.";
RL   Cell 123:265-276(2005).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-231, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Cervix carcinoma;
RX   PubMed=17924679; DOI=10.1021/pr070152u;
RA   Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D.;
RT   "Improved titanium dioxide enrichment of phosphopeptides from HeLa
RT   cells and high confident phosphopeptide identification by cross-
RT   validation of MS/MS and MS/MS/MS spectra.";
RL   J. Proteome Res. 6:4150-4162(2007).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-231, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Cervix carcinoma;
RX   PubMed=18691976; DOI=10.1016/j.molcel.2008.07.007;
RA   Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,
RA   Greff Z., Keri G., Stemmann O., Mann M.;
RT   "Kinase-selective enrichment enables quantitative phosphoproteomics of
RT   the kinome across the cell cycle.";
RL   Mol. Cell 31:438-448(2008).
RN   [8]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-231, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Leukemic T-cell;
RX   PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA   Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA   Rodionov V., Han D.K.;
RT   "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT   reveals system-wide modulation of protein-protein interactions.";
RL   Sci. Signal. 2:RA46-RA46(2009).
CC   -!- FUNCTION: Component of the Integrator complex, a complex involved
CC       in the small nuclear RNAs (snRNA) U1 and U2 transcription and in
CC       their 3'-box-dependent processing. The Integrator complex is
CC       associated with the C-terminal domain (CTD) of RNA polymerase II
CC       largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs
CC       genes.
CC   -!- SUBUNIT: Belongs to the multiprotein complex Integrator, at least
CC       composed of INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7,
CC       INTS8, INTS9/RC74, INTS10, CPSF3L/INTS11 and INTS12. Interacts
CC       with POLR2A.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- SIMILARITY: Belongs to the Integrator subunit 10 family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA91687.1; Type=Frameshift; Positions=90;
CC       Sequence=CAG33543.1; Type=Erroneous initiation;
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DR   EMBL; AK001431; BAA91687.1; ALT_FRAME; mRNA.
DR   EMBL; BC001915; AAH01915.2; -; mRNA.
DR   EMBL; BC006209; AAH06209.2; -; mRNA.
DR   EMBL; BC051282; AAH51282.2; -; mRNA.
DR   EMBL; CR457262; CAG33543.1; ALT_INIT; mRNA.
DR   EMBL; AL442078; CAC09444.1; -; mRNA.
DR   EMBL; BK005727; DAA05727.1; ALT_FRAME; mRNA.
DR   IPI; IPI00550272; -.
DR   RefSeq; NP_060612.2; NM_018142.2.
DR   UniGene; Hs.512627; -.
DR   ProteinModelPortal; Q9NVR2; -.
DR   DIP; DIP-34063N; -.
DR   IntAct; Q9NVR2; 1.
DR   STRING; Q9NVR2; -.
DR   PhosphoSite; Q9NVR2; -.
DR   DMDM; 97052274; -.
DR   PRIDE; Q9NVR2; -.
DR   Ensembl; ENST00000397977; ENSP00000381064; ENSG00000104613.
DR   GeneID; 55174; -.
DR   KEGG; hsa:55174; -.
DR   UCSC; uc003wzj.1; human.
DR   CTD; 55174; -.
DR   GeneCards; GC08P019674; -.
DR   H-InvDB; HIX0007350; -.
DR   HGNC; HGNC:25548; INTS10.
DR   HPA; HPA039129; -.
DR   MIM; 611353; gene.
DR   neXtProt; NX_Q9NVR2; -.
DR   PharmGKB; PA142672354; -.
DR   eggNOG; prNOG10124; -.
DR   GeneTree; ENSGT00390000010950; -.
DR   HOGENOM; HBG444146; -.
DR   HOVERGEN; HBG081797; -.
DR   InParanoid; Q9NVR2; -.
DR   OMA; DDEDCSA; -.
DR   OrthoDB; EOG43FGWD; -.
DR   PhylomeDB; Q9NVR2; -.
DR   NextBio; 58967; -.
DR   ArrayExpress; Q9NVR2; -.
DR   Bgee; Q9NVR2; -.
DR   CleanEx; HS_INTS10; -.
DR   Genevestigator; Q9NVR2; -.
DR   GermOnline; ENSG00000104613; Homo sapiens.
DR   GO; GO:0005737; C:cytoplasm; IDA:HPA.
DR   GO; GO:0032039; C:integrator complex; IDA:HGNC.
DR   GO; GO:0016180; P:snRNA processing; IDA:HGNC.
DR   KO; K13147; -.
PE   1: Evidence at protein level;
KW   Complete proteome; Nucleus; Phosphoprotein; Reference proteome.
FT   CHAIN         1    710       Integrator complex subunit 10.
FT                                /FTId=PRO_0000235687.
FT   COMPBIAS    132    136       Poly-Leu.
FT   MOD_RES     231    231       Phosphoserine.
FT   CONFLICT    412    412       S -> N (in Ref. 3; CAG33543).
SQ   SEQUENCE   710 AA;  82236 MW;  3E9945064EC4BEFB CRC64;
     MSAQGDCEFL VQRARELVPQ DLWAAKAWLI TARSLYPADF NIQYEMYTIE RNAERTATAG
     RLLYDMFVNF PDQPVVWREI SIITSALRND SQDKQTQFLR SLFETLPGRV QCEMLLKVTE
     QCFNTLERSE MLLLLLRRFP ETVVQHGVGL GEALLEAETI EEQESPVNCF RKLFVCDVLP
     LIINNHDVRL PANLLYKYLN KAAEFYINYV TRSTQIENQH QGAQDTSDLM SPSKRSSQKY
     IIEGLTEKSS QIVDPWERLF KILNVVGMRC EWQMDKGRRS YGDILHRMKD LCRYMNNFDS
     EAHAKYKNQV VYSTMLVFFK NAFQYVNSIQ PSLFQGPNAP SQVPLVLLED VSNVYGDVEI
     DRNKHIHKKR KLAEGREKTM SSDDEDCSAK GRNRHIVVNK AELANSTEVL ESFKLARESW
     ELLYSLEFLD KEFTRICLAW KTDTWLWLRI FLTDMIIYQG QYKKAIASLH HLAALQGSIS
     QPQITGQGTL EHQRALIQLA TCHFALGEYR MTCEKVLDLM CYMVLPIQDG GKSQEEPSKV
     KPKFRKGSDL KLLPCTSKAI MPYCLHLMLA CFKLRAFTDN RDDMALGHVI VLLQQEWPRG
     ENLFLKAVNK ICQQGNFQYE NFFNYVTNID MLEEFAYLRT QEGGKIHLEL LPNQGMLIKH
     HTVTRGITKG VKEDFRLAME RQVSRCGENL MVVLHRFCIN EKILLLQTLT
//