Reviewed, UniProtKB/Swiss-Prot Q9NVR2 (INT10_HUMAN)

Names and origin

Protein names

Recommended name:
    Integrator complex subunit 10
      Short name=Int10

Gene names

Name:	INTS10
Synonyms:	C8orf35

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	710 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.
Subunit structure	Belongs to the multiprotein complex Integrator, at least composed of INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7, INTS8, INTS9/RC74, INTS10, CPSF3L/INTS11 and INTS12. Interacts with POLR2A.
Subcellular location	Nucleus. Ref.5
Sequence similarities	Belongs to the Integrator subunit 10 family.
Sequence caution	The sequence BAA91687.1 differs from that shown. Reason: Frameshift at position 90.

Ontologies

Keywords
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	snRNA processing Ref.5 Inferred from direct assay. Source: HGNC
Cellular component	integrator complex Ref.5 Inferred from direct assay. Source: HGNC
Molecular function	protein binding Ref.5 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RAF1	P04049	2	EBI-536703,EBI-365996

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 710	710	Integrator complex subunit 10		PRO_0000235687
Regions
	Compositional bias	132 – 136	5	Poly-Leu
Amino acid modifications
	Modified residue	231	1	Phosphoserine Ref.6 Ref.7
Experimental info
	Sequence conflict	412	1	S → N in CAG33543. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9NVR2-1 [UniParc]. Last modified May 16, 2006. Version 2. Checksum: 3E9945064EC4BEFB	FASTA	710	82,236
	10 20 30 40 50 60 MSAQGDCEFL VQRARELVPQ DLWAAKAWLI TARSLYPADF NIQYEMYTIE RNAERTATAG 70 80 90 100 110 120 RLLYDMFVNF PDQPVVWREI SIITSALRND SQDKQTQFLR SLFETLPGRV QCEMLLKVTE 130 140 150 160 170 180 QCFNTLERSE MLLLLLRRFP ETVVQHGVGL GEALLEAETI EEQESPVNCF RKLFVCDVLP 190 200 210 220 230 240 LIINNHDVRL PANLLYKYLN KAAEFYINYV TRSTQIENQH QGAQDTSDLM SPSKRSSQKY 250 260 270 280 290 300 IIEGLTEKSS QIVDPWERLF KILNVVGMRC EWQMDKGRRS YGDILHRMKD LCRYMNNFDS 310 320 330 340 350 360 EAHAKYKNQV VYSTMLVFFK NAFQYVNSIQ PSLFQGPNAP SQVPLVLLED VSNVYGDVEI 370 380 390 400 410 420 DRNKHIHKKR KLAEGREKTM SSDDEDCSAK GRNRHIVVNK AELANSTEVL ESFKLARESW 430 440 450 460 470 480 ELLYSLEFLD KEFTRICLAW KTDTWLWLRI FLTDMIIYQG QYKKAIASLH HLAALQGSIS 490 500 510 520 530 540 QPQITGQGTL EHQRALIQLA TCHFALGEYR MTCEKVLDLM CYMVLPIQDG GKSQEEPSKV 550 560 570 580 590 600 KPKFRKGSDL KLLPCTSKAI MPYCLHLMLA CFKLRAFTDN RDDMALGHVI VLLQQEWPRG 610 620 630 640 650 660 ENLFLKAVNK ICQQGNFQYE NFFNYVTNID MLEEFAYLRT QEGGKIHLEL LPNQGMLIKH 670 680 690 700 710 HTVTRGITKG VKEDFRLAME RQVSRCGENL MVVLHRFCIN EKILLLQTLT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Teratocarcinoma.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 74-710. Tissue: Lung and Neuroblastoma.
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 114-710.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 377-710. Tissue: Lymph node.
[5]	"Integrator, a multiprotein mediator of small nuclear RNA processing, associates with the C-terminal repeat of RNA polymerase II." Baillat D., Hakimi M.-A., Naeaer A.M., Shilatifard A., Cooch N., Shiekhattar R. Cell 123:265-276(2005) [PubMed: 16239144] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE INTEGRATOR COMPLEX, SUBCELLULAR LOCATION.
[6]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-231, MASS SPECTROMETRY. Tissue: Epithelium.
[7]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-231, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK001431 mRNA. Translation: BAA91687.1. Frameshift. BC001915 mRNA. Translation: AAH01915.1. Different initiation. BC006209 mRNA. Translation: AAH06209.2. BC051282 mRNA. Translation: AAH51282.1. Different initiation. CR457262 mRNA. Translation: CAG33543.1. Different initiation. AL442078 mRNA. Translation: CAC09444.1. BK005727 mRNA. Translation: DAA05727.1. Frameshift.
IPI	IPI00550272.
RefSeq	NP_060612.2.
UniGene	Hs.512627
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NVR2. 1 interaction.
PTM databases
PhosphoSite	Q9NVR2.
Proteomic databases
PRIDE	Q9NVR2.
Genome annotation databases
Ensembl	ENSG00000104613. Homo sapiens. [Contig view]
GeneID	55174.
KEGG	hsa:55174.
UCSC	uc003wzj.1. human.
Organism-specific databases
GeneCards	GC08P019721.
HGNC	HGNC:25548. INTS10.
MIM	611353. gene.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9NVR2.
HOVERGEN	Q9NVR2.
OMA	Q9NVR2. DDEDCSA.
Gene expression databases
ArrayExpress	Q9NVR2.
Bgee	Q9NVR2.
CleanEx	HS_INTS10.
GermOnline	ENSG00000104613. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	58967.
SOURCE	Search...

Entry information

Entry name

INT10_HUMAN

Accession

Primary (citable) accession number: Q9NVR2
Secondary accession number(s): Q6IA93 , Q7L538, Q7L8C8, Q9H3W8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	May 16, 2006
Last modified:	July 7, 2009
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)