Histone chaperone ASF1B - Homo sapiens (Human)

Names and origin

Protein names

Recommended name:
    Histone chaperone ASF1B
Alternative name(s):
    Anti-silencing function protein 1 homolog B
      Short name=hAsf1
      Short name=hAsf1b
    CCG1-interacting factor A-II
      Short name=CIA-II
      Short name=hCIA-II

Gene names

Name:

ASF1B

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis. Ref.2 Ref.7 Ref.8 Ref.9 Ref.10
Subunit structure	Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex. Interacts with HAT1, NASP, TAF1, TLK1 and TLK2. Ref.2 Ref.7 Ref.10 Ref.1 Ref.11 Ref.12
Subcellular location	Nucleus. Ref.2
Tissue specificity	Highly expressed in testis and at lower levels in colon, small intestine and thymus. Ref.2
Post-translational modification	Phosphorylated by TLK1 and TLK2. Ref.1 Ref.13
Sequence similarities	Belongs to the ASF1 family.

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Ontologies

Keywords
Biological process	Differentiation Spermatogenesis Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Chaperone Chromatin regulator Developmental protein
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW spermatogenesis Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
C15orf41	Q9Y2V0	1	EBI-1055650,EBI-1047601
C6orf167	Q6ZRQ5	1	EBI-1055650,EBI-718662
CDAN1	Q8IWY9	1	EBI-1055650,EBI-726307
CHAF1A	Q13111	1	EBI-1055650,EBI-1020839
CHAF1B	Q13112	1	EBI-1055650,EBI-1052944
DOK2	O60496	1	EBI-1055650,EBI-1046024
DYNLL1	P63167	1	EBI-1055650,EBI-349105
HIST1H3A	P68431	1	EBI-1055650,EBI-79722
IPO4	Q8TEX9	1	EBI-1055650,EBI-395967
MRPS7	Q9Y2R9	1	EBI-1055650,EBI-1054973
NFKBIL2	Q96HA7	1	EBI-1055650,EBI-1052467
PSPH	P78330	1	EBI-1055650,EBI-1042956
RBBP4	Q09028	1	EBI-1055650,EBI-620823
RPL31	P62899	1	EBI-1055650,EBI-1053664
RPS3A	P61247	1	EBI-1055650,EBI-352378
TLK1	Q9UKI8	1	EBI-1055650,EBI-740492
TLK2	Q86UE8	1	EBI-1055650,EBI-1047967
TTN	Q8WZ42	1	EBI-1055650,EBI-681210

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 202

202

Histone chaperone ASF1B

PRO_0000284015

Regions

Region

1 – 156

156

Interaction with histone H3 By similarity

Region

1 – 155

155

Interaction with CHAF1B

Amino acid modifications

Modified residue

142

1

Phosphoserine Ref.13

Experimental info

Sequence conflict

11

1

V → A in BAA91602. Ref.4

Sequence conflict

23

1

R → Q in BAD96800. Ref.5

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Sequences

Sequence

Length

Mass (Da)

Tools

Q9NVP2-1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: BD62F726610E3A70
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FASTA

202

22,434

        10         20         30         40         50         60 
MAKVSVLNVA VLENPSPFHS PFRFEISFEC SEALADDLEW KIIYVGSAES EEFDQILDSV 

        70         80         90        100        110        120 
LVGPVPAGRH MFVFQADAPN PSLIPETDAV GVTVVLITCT YHGQEFIRVG YYVNNEYLNP 

       130        140        150        160        170        180 
ELRENPPMKP DFSQLQRNIL ASNPRVTRFH INWDNNMDRL EAIETQDPSL GCGLPLNCTP 

       190        200 
IKGLGLPGCI PGLLPENSMD CI

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases." Sillje H.H.W., Nigg E.A. Curr. Biol. 11:1068-1073(2001) [PubMed: 11470414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH TLK1 AND TLK2, PHOSPHORYLATION BY TLK1 AND TLK2.
[2]	"Transcription initiation factor IID-interactive histone chaperone CIA-II implicated in mammalian spermatogenesis." Umehara T., Horikoshi M. J. Biol. Chem. 278:35660-35667(2003) [PubMed: 12842904] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH HISTONE H3.3; HISTONE H4 AND TAF1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Teratocarcinoma.
[5]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis and Uterus.
[7]	"Human Asf1 and CAF-1 interact and synergize in a repair-coupled nucleosome assembly pathway." Mello J.A., Sillje H.H.W., Roche D.M.J., Kirschner D.B., Nigg E.A., Almouzni G. EMBO Rep. 3:329-334(2002) [PubMed: 11897662] [Abstract] Cited for: FUNCTION, INTERACTION WITH CHAF1A; CHAF1B AND RBBP4.
[8]	"Histone H3.1 and H3.3 complexes mediate nucleosome assembly pathways dependent or independent of DNA synthesis." Tagami H., Ray-Gallet D., Almouzni G., Nakatani Y. Cell 116:51-61(2004) [PubMed: 14718166] [Abstract] Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN COMPLEXES WITH CHAF1A; CHAF1B; HAT1; HISTONE H3.1; HISTONE H3.3; HISTONE H4; NASP AND RBBP4.
[9]	"Functional conservation and specialization among eukaryotic anti-silencing function 1 histone chaperones." Tamburini B.A., Carson J.J., Adkins M.W., Tyler J.K. Eukaryot. Cell 4:1583-1590(2005) [PubMed: 16151251] [Abstract] Cited for: FUNCTION.
[10]	"Human Asf1 regulates the flow of S phase histones during replicational stress." Groth A., Ray-Gallet D., Quivy J.-P., Lukas J., Bartek J., Almouzni G. Mol. Cell 17:301-311(2005) [PubMed: 15664198] [Abstract] Cited for: FUNCTION, INTERACTION WITH HISTONE H3.1; HISTONE H3.3 AND HISTONE H4.
[11]	"Asf1 is required for viability and chromatin assembly during DNA replication in vertebrate cells." Sanematsu F., Takami Y., Barman H.K., Fukagawa T., Ono T., Shibahara K., Nakayama T. J. Biol. Chem. 281:13817-13827(2006) [PubMed: 16537536] [Abstract] Cited for: INTERACTION WITH CHAF1B; HISTONE H3 AND HISTONE H4.
[12]	"Structure of a human ASF1a-HIRA complex and insights into specificity of histone chaperone complex assembly." Tang Y., Poustovoitov M.V., Zhao K., Garfinkel M., Canutescu A., Dunbrack R., Adams P.D., Marmorstein R. Nat. Struct. Mol. Biol. 13:921-929(2006) [PubMed: 16980972] [Abstract] Cited for: INTERACTION WITH CHAF1B.
[13]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142, MASS SPECTROMETRY.
[14]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	AF279307 mRNA. Translation: AAK82973.1. AB104486 mRNA. Translation: BAC87709.1. CR457235 mRNA. Translation: CAG33516.1. AK001288 mRNA. Translation: BAA91602.1. AK001466 mRNA. Translation: BAA91708.1. AK223080 mRNA. Translation: BAD96800.1. BC007726 mRNA. Translation: AAH07726.1. BC010014 mRNA. Translation: AAH10014.1. BC036521 mRNA. Translation: AAH36521.1.
IPI	IPI00041127.
RefSeq	NP_060624.1.
UniGene	Hs.26516
3D structure databases
HSSP	HSSP built from PDB template 1ROC based on UniProtKB P32447.
SMR	Q9NVP2. Positions 1-154.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NVP2. 22 interactions.
PTM databases
PhosphoSite	Q9NVP2.
Proteomic databases
PeptideAtlas	Q9NVP2.
PRIDE	Q9NVP2.
Genome annotation databases
Ensembl	ENSG00000105011. Homo sapiens. [Contig view]
GeneID	55723.
KEGG	hsa:55723.
Organism-specific databases
GeneCards	GC19M014091.
HGNC	HGNC:20996. ASF1B.
MIM	609190. gene.
PharmGKB	PA134931112.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q9NVP2.
OMA	Q9NVP2. LSCTPVK.
Gene expression databases
ArrayExpress	Q9NVP2.
Bgee	Q9NVP2.
CleanEx	HS_ASF1B.
Family and domain databases
InterPro	IPR006818. Histone_chaperone_ASF1-like. [Graphical view]
Gene3D	G3DSA:2.60.40.1490. Anti-silence. 1 hit.
PANTHER	PTHR12040. Anti-silence. 1 hit.
Pfam	PF04729. ASF1_hist_chap. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	60631.
SOURCE	Search...

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Entry information

Entry name

ASF1B_HUMAN

Accession

Primary (citable) accession number: Q9NVP2
Secondary accession number(s): Q53G51, Q9NVZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents