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Q9NVP1

- DDX18_HUMAN

UniProt

Q9NVP1 - DDX18_HUMAN

Protein

ATP-dependent RNA helicase DDX18

Gene

DDX18

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 2 (10 May 2002)
      Previous versions | rss
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    Functioni

    Probable RNA-dependent helicase.

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi223 – 2308ATPCurated

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent RNA helicase activity Source: ProtInc
    3. poly(A) RNA binding Source: UniProtKB
    4. protein binding Source: UniProtKB

    GO - Biological processi

    1. ATP catabolic process Source: GOC

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent RNA helicase DDX18 (EC:3.6.4.13)
    Alternative name(s):
    DEAD box protein 18
    Myc-regulated DEAD box protein
    Short name:
    MrDb
    Gene namesi
    Name:DDX18
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:2741. DDX18.

    Subcellular locationi

    Nucleusnucleolus 1 Publication

    GO - Cellular componenti

    1. membrane Source: UniProtKB
    2. nucleolus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA27207.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 670670ATP-dependent RNA helicase DDX18PRO_0000055001Add
    BLAST

    Proteomic databases

    MaxQBiQ9NVP1.
    PaxDbiQ9NVP1.
    PeptideAtlasiQ9NVP1.
    PRIDEiQ9NVP1.

    2D gel databases

    SWISS-2DPAGEQ9NVP1.

    PTM databases

    PhosphoSiteiQ9NVP1.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9NVP1.
    BgeeiQ9NVP1.
    CleanExiHS_DDX18.
    GenevestigatoriQ9NVP1.

    Organism-specific databases

    HPAiHPA035400.
    HPA041056.

    Interactioni

    Subunit structurei

    Interacts with NOL8; the interaction is RNA-dependent.2 Publications

    Protein-protein interaction databases

    BioGridi114404. 23 interactions.
    IntActiQ9NVP1. 7 interactions.
    MINTiMINT-1382348.
    STRINGi9606.ENSP00000263239.

    Structurei

    Secondary structure

    1
    670
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi175 – 1773
    Helixi188 – 1969
    Helixi204 – 21512
    Helixi229 – 24315
    Helixi248 – 2503
    Beta strandi254 – 2574
    Helixi261 – 27414
    Turni275 – 2773
    Beta strandi282 – 2854
    Beta strandi287 – 2893
    Helixi291 – 30010
    Beta strandi303 – 3075
    Helixi309 – 31810
    Beta strandi329 – 3324
    Helixi335 – 3406
    Helixi344 – 35310
    Beta strandi356 – 3638
    Helixi369 – 37810

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3LY5X-ray2.80A/B149-387[»]
    ProteinModelPortaliQ9NVP1.
    SMRiQ9NVP1. Positions 169-614.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9NVP1.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini210 – 385176Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini399 – 569171Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi179 – 20729Q motifAdd
    BLAST
    Motifi333 – 3364DEAD box

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi108 – 1169Poly-Lys
    Compositional biasi636 – 6427Poly-Gly

    Sequence similaritiesi

    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0513.
    HOGENOMiHOG000268799.
    HOVERGENiHBG105702.
    InParanoidiQ9NVP1.
    KOiK13179.
    OMAiKGFVFRN.
    OrthoDBiEOG7GFB4K.
    PhylomeDBiQ9NVP1.
    TreeFamiTF300471.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR025313. DUF4217.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000629. RNA-helicase_DEAD-box_CS.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF13959. DUF4217. 1 hit.
    PF00271. Helicase_C. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9NVP1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSHLPMKLLR KKIEKRNLKL RQRNLKFQGA SNLTLSETQN GDVSEETMGS    50
    RKVKKSKQKP MNVGLSETQN GGMSQEAVGN IKVTKSPQKS TVLTNGEAAM 100
    QSSNSESKKK KKKKRKMVND AEPDTKKAKT ENKGKSEEES AETTKETENN 150
    VEKPDNDEDE SEVPSLPLGL TGAFEDTSFA SLCNLVNENT LKAIKEMGFT 200
    NMTEIQHKSI RPLLEGRDLL AAAKTGSGKT LAFLIPAVEL IVKLRFMPRN 250
    GTGVLILSPT RELAMQTFGV LKELMTHHVH TYGLIMGGSN RSAEAQKLGN 300
    GINIIVATPG RLLDHMQNTP GFMYKNLQCL VIDEADRILD VGFEEELKQI 350
    IKLLPTRRQT MLFSATQTRK VEDLARISLK KEPLYVGVDD DKANATVDGL 400
    EQGYVVCPSE KRFLLLFTFL KKNRKKKLMV FFSSCMSVKY HYELLNYIDL 450
    PVLAIHGKQK QNKRTTTFFQ FCNADSGTLL CTDVAARGLD IPEVDWIVQY 500
    DPPDDPKEYI HRVGRTARGL NGRGHALLIL RPEELGFLRY LKQSKVPLSE 550
    FDFSWSKISD IQSQLEKLIE KNYFLHKSAQ EAYKSYIRAY DSHSLKQIFN 600
    VNNLNLPQVA LSFGFKVPPF VDLNVNSNEG KQKKRGGGGG FGYQKTKKVE 650
    KSKIFKHISK KSSDSRQFSH 670
    Length:670
    Mass (Da):75,407
    Last modified:May 10, 2002 - v2
    Checksum:i2FE653A98751F9DC
    GO

    Sequence cautioni

    The sequence CAA67295.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti248 – 2481P → T in BAA91709. (PubMed:14702039)Curated
    Sequence conflicti382 – 3821E → G in CAG33341. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti41 – 411G → R in a breast cancer sample; somatic mutation. 1 Publication
    VAR_035841
    Natural varianti94 – 941T → S.2 Publications
    Corresponds to variant rs1052637 [ dbSNP | Ensembl ].
    VAR_013293
    Natural varianti647 – 6471K → R.
    Corresponds to variant rs10179772 [ dbSNP | Ensembl ].
    VAR_033857

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK001467 mRNA. Translation: BAA91709.1.
    BC001238 mRNA. Translation: AAH01238.1.
    BC003360 mRNA. Translation: AAH03360.1.
    BC024739 mRNA. Translation: AAH24739.1.
    X98743 mRNA. Translation: CAA67295.1. Different initiation.
    CR457060 mRNA. Translation: CAG33341.1.
    CCDSiCCDS2120.1.
    PIRiS71758.
    RefSeqiNP_006764.3. NM_006773.3.
    UniGeneiHs.744922.

    Genome annotation databases

    EnsembliENST00000263239; ENSP00000263239; ENSG00000088205.
    GeneIDi8886.
    KEGGihsa:8886.
    UCSCiuc002tlh.1. human.

    Polymorphism databases

    DMDMi20532388.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK001467 mRNA. Translation: BAA91709.1 .
    BC001238 mRNA. Translation: AAH01238.1 .
    BC003360 mRNA. Translation: AAH03360.1 .
    BC024739 mRNA. Translation: AAH24739.1 .
    X98743 mRNA. Translation: CAA67295.1 . Different initiation.
    CR457060 mRNA. Translation: CAG33341.1 .
    CCDSi CCDS2120.1.
    PIRi S71758.
    RefSeqi NP_006764.3. NM_006773.3.
    UniGenei Hs.744922.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3LY5 X-ray 2.80 A/B 149-387 [» ]
    ProteinModelPortali Q9NVP1.
    SMRi Q9NVP1. Positions 169-614.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114404. 23 interactions.
    IntActi Q9NVP1. 7 interactions.
    MINTi MINT-1382348.
    STRINGi 9606.ENSP00000263239.

    PTM databases

    PhosphoSitei Q9NVP1.

    Polymorphism databases

    DMDMi 20532388.

    2D gel databases

    SWISS-2DPAGE Q9NVP1.

    Proteomic databases

    MaxQBi Q9NVP1.
    PaxDbi Q9NVP1.
    PeptideAtlasi Q9NVP1.
    PRIDEi Q9NVP1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000263239 ; ENSP00000263239 ; ENSG00000088205 .
    GeneIDi 8886.
    KEGGi hsa:8886.
    UCSCi uc002tlh.1. human.

    Organism-specific databases

    CTDi 8886.
    GeneCardsi GC02P118572.
    HGNCi HGNC:2741. DDX18.
    HPAi HPA035400.
    HPA041056.
    MIMi 606355. gene.
    neXtProti NX_Q9NVP1.
    PharmGKBi PA27207.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0513.
    HOGENOMi HOG000268799.
    HOVERGENi HBG105702.
    InParanoidi Q9NVP1.
    KOi K13179.
    OMAi KGFVFRN.
    OrthoDBi EOG7GFB4K.
    PhylomeDBi Q9NVP1.
    TreeFami TF300471.

    Miscellaneous databases

    ChiTaRSi DDX18. human.
    EvolutionaryTracei Q9NVP1.
    GenomeRNAii 8886.
    NextBioi 33369.
    PROi Q9NVP1.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9NVP1.
    Bgeei Q9NVP1.
    CleanExi HS_DDX18.
    Genevestigatori Q9NVP1.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR025313. DUF4217.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000629. RNA-helicase_DEAD-box_CS.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF13959. DUF4217. 1 hit.
    PF00271. Helicase_C. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00039. DEAD_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-94.
      Tissue: Placenta.
    3. "Myc-Max heterodimers activate a DEAD box gene and interact with multiple E box-related sites in vivo."
      Grandori C., Mac J., Siebelt F., Ayer D.A., Eisenman R.E.
      EMBO J. 15:4344-4357(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 56-670.
    4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 61-670, VARIANT SER-94.
    5. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
      Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
      Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 370-376.
      Tissue: Platelet.
    6. "NOP132 is required for proper nucleolus localization of DEAD-box RNA helicase DDX47."
      Sekiguchi T., Hayano T., Yanagida M., Takahashi N., Nishimoto T.
      Nucleic Acids Res. 34:4593-4608(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NOL8, SUBCELLULAR LOCATION.
    7. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
      Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 149-387 IN COMPLEX WITH PHOSPHATE.
    10. Cited for: VARIANT [LARGE SCALE ANALYSIS] ARG-41.

    Entry informationi

    Entry nameiDDX18_HUMAN
    AccessioniPrimary (citable) accession number: Q9NVP1
    Secondary accession number(s): Q6GTZ9
    , Q6IAU4, Q92732, Q9BQB7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: May 10, 2002
    Last modified: October 1, 2014
    This is version 132 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3