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Q9NVP1

- DDX18_HUMAN

UniProt

Q9NVP1 - DDX18_HUMAN

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Protein

ATP-dependent RNA helicase DDX18

Gene

DDX18

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Probable RNA-dependent helicase.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi223 – 2308ATPCurated

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent RNA helicase activity Source: ProtInc
  3. poly(A) RNA binding Source: UniProtKB

GO - Biological processi

  1. ATP catabolic process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DDX18 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 18
Myc-regulated DEAD box protein
Short name:
MrDb
Gene namesi
Name:DDX18
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 2

Organism-specific databases

HGNCiHGNC:2741. DDX18.

Subcellular locationi

Nucleusnucleolus 1 Publication

GO - Cellular componenti

  1. membrane Source: UniProtKB
  2. nucleolus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27207.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 670670ATP-dependent RNA helicase DDX18PRO_0000055001Add
BLAST

Proteomic databases

MaxQBiQ9NVP1.
PaxDbiQ9NVP1.
PeptideAtlasiQ9NVP1.
PRIDEiQ9NVP1.

2D gel databases

SWISS-2DPAGEQ9NVP1.

PTM databases

PhosphoSiteiQ9NVP1.

Expressioni

Gene expression databases

BgeeiQ9NVP1.
CleanExiHS_DDX18.
ExpressionAtlasiQ9NVP1. baseline and differential.
GenevestigatoriQ9NVP1.

Organism-specific databases

HPAiHPA035400.
HPA041056.

Interactioni

Subunit structurei

Interacts with NOL8; the interaction is RNA-dependent.2 Publications

Protein-protein interaction databases

BioGridi114404. 29 interactions.
IntActiQ9NVP1. 7 interactions.
MINTiMINT-1382348.
STRINGi9606.ENSP00000263239.

Structurei

Secondary structure

1
670
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi175 – 1773Combined sources
Helixi188 – 1969Combined sources
Helixi204 – 21512Combined sources
Helixi229 – 24315Combined sources
Helixi248 – 2503Combined sources
Beta strandi254 – 2574Combined sources
Helixi261 – 27414Combined sources
Turni275 – 2773Combined sources
Beta strandi282 – 2854Combined sources
Beta strandi287 – 2893Combined sources
Helixi291 – 30010Combined sources
Beta strandi303 – 3075Combined sources
Helixi309 – 31810Combined sources
Beta strandi329 – 3324Combined sources
Helixi335 – 3406Combined sources
Helixi344 – 35310Combined sources
Beta strandi356 – 3638Combined sources
Helixi369 – 37810Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3LY5X-ray2.80A/B149-387[»]
ProteinModelPortaliQ9NVP1.
SMRiQ9NVP1. Positions 169-614.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NVP1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini210 – 385176Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini399 – 569171Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi179 – 20729Q motifAdd
BLAST
Motifi333 – 3364DEAD box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi108 – 1169Poly-Lys
Compositional biasi636 – 6427Poly-Gly

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0513.
GeneTreeiENSGT00680000100037.
HOGENOMiHOG000268799.
HOVERGENiHBG105702.
InParanoidiQ9NVP1.
KOiK13179.
OMAiKGFVFRN.
OrthoDBiEOG7GFB4K.
PhylomeDBiQ9NVP1.
TreeFamiTF300471.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR025313. DUF4217.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF13959. DUF4217. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9NVP1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSHLPMKLLR KKIEKRNLKL RQRNLKFQGA SNLTLSETQN GDVSEETMGS
60 70 80 90 100
RKVKKSKQKP MNVGLSETQN GGMSQEAVGN IKVTKSPQKS TVLTNGEAAM
110 120 130 140 150
QSSNSESKKK KKKKRKMVND AEPDTKKAKT ENKGKSEEES AETTKETENN
160 170 180 190 200
VEKPDNDEDE SEVPSLPLGL TGAFEDTSFA SLCNLVNENT LKAIKEMGFT
210 220 230 240 250
NMTEIQHKSI RPLLEGRDLL AAAKTGSGKT LAFLIPAVEL IVKLRFMPRN
260 270 280 290 300
GTGVLILSPT RELAMQTFGV LKELMTHHVH TYGLIMGGSN RSAEAQKLGN
310 320 330 340 350
GINIIVATPG RLLDHMQNTP GFMYKNLQCL VIDEADRILD VGFEEELKQI
360 370 380 390 400
IKLLPTRRQT MLFSATQTRK VEDLARISLK KEPLYVGVDD DKANATVDGL
410 420 430 440 450
EQGYVVCPSE KRFLLLFTFL KKNRKKKLMV FFSSCMSVKY HYELLNYIDL
460 470 480 490 500
PVLAIHGKQK QNKRTTTFFQ FCNADSGTLL CTDVAARGLD IPEVDWIVQY
510 520 530 540 550
DPPDDPKEYI HRVGRTARGL NGRGHALLIL RPEELGFLRY LKQSKVPLSE
560 570 580 590 600
FDFSWSKISD IQSQLEKLIE KNYFLHKSAQ EAYKSYIRAY DSHSLKQIFN
610 620 630 640 650
VNNLNLPQVA LSFGFKVPPF VDLNVNSNEG KQKKRGGGGG FGYQKTKKVE
660 670
KSKIFKHISK KSSDSRQFSH
Length:670
Mass (Da):75,407
Last modified:May 10, 2002 - v2
Checksum:i2FE653A98751F9DC
GO

Sequence cautioni

The sequence CAA67295.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti248 – 2481P → T in BAA91709. (PubMed:14702039)Curated
Sequence conflicti382 – 3821E → G in CAG33341. 1 PublicationCurated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti41 – 411G → R in a breast cancer sample; somatic mutation. 1 Publication
VAR_035841
Natural varianti94 – 941T → S.2 Publications
Corresponds to variant rs1052637 [ dbSNP | Ensembl ].
VAR_013293
Natural varianti647 – 6471K → R.
Corresponds to variant rs10179772 [ dbSNP | Ensembl ].
VAR_033857

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001467 mRNA. Translation: BAA91709.1.
BC001238 mRNA. Translation: AAH01238.1.
BC003360 mRNA. Translation: AAH03360.1.
BC024739 mRNA. Translation: AAH24739.1.
X98743 mRNA. Translation: CAA67295.1. Different initiation.
CR457060 mRNA. Translation: CAG33341.1.
CCDSiCCDS2120.1.
PIRiS71758.
RefSeqiNP_006764.3. NM_006773.3.
UniGeneiHs.744922.

Genome annotation databases

EnsembliENST00000263239; ENSP00000263239; ENSG00000088205.
GeneIDi8886.
KEGGihsa:8886.
UCSCiuc002tlh.1. human.

Polymorphism databases

DMDMi20532388.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001467 mRNA. Translation: BAA91709.1 .
BC001238 mRNA. Translation: AAH01238.1 .
BC003360 mRNA. Translation: AAH03360.1 .
BC024739 mRNA. Translation: AAH24739.1 .
X98743 mRNA. Translation: CAA67295.1 . Different initiation.
CR457060 mRNA. Translation: CAG33341.1 .
CCDSi CCDS2120.1.
PIRi S71758.
RefSeqi NP_006764.3. NM_006773.3.
UniGenei Hs.744922.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3LY5 X-ray 2.80 A/B 149-387 [» ]
ProteinModelPortali Q9NVP1.
SMRi Q9NVP1. Positions 169-614.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 114404. 29 interactions.
IntActi Q9NVP1. 7 interactions.
MINTi MINT-1382348.
STRINGi 9606.ENSP00000263239.

PTM databases

PhosphoSitei Q9NVP1.

Polymorphism databases

DMDMi 20532388.

2D gel databases

SWISS-2DPAGE Q9NVP1.

Proteomic databases

MaxQBi Q9NVP1.
PaxDbi Q9NVP1.
PeptideAtlasi Q9NVP1.
PRIDEi Q9NVP1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000263239 ; ENSP00000263239 ; ENSG00000088205 .
GeneIDi 8886.
KEGGi hsa:8886.
UCSCi uc002tlh.1. human.

Organism-specific databases

CTDi 8886.
GeneCardsi GC02P118572.
HGNCi HGNC:2741. DDX18.
HPAi HPA035400.
HPA041056.
MIMi 606355. gene.
neXtProti NX_Q9NVP1.
PharmGKBi PA27207.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0513.
GeneTreei ENSGT00680000100037.
HOGENOMi HOG000268799.
HOVERGENi HBG105702.
InParanoidi Q9NVP1.
KOi K13179.
OMAi KGFVFRN.
OrthoDBi EOG7GFB4K.
PhylomeDBi Q9NVP1.
TreeFami TF300471.

Miscellaneous databases

ChiTaRSi DDX18. human.
EvolutionaryTracei Q9NVP1.
GenomeRNAii 8886.
NextBioi 33369.
PROi Q9NVP1.
SOURCEi Search...

Gene expression databases

Bgeei Q9NVP1.
CleanExi HS_DDX18.
ExpressionAtlasi Q9NVP1. baseline and differential.
Genevestigatori Q9NVP1.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR011545. DEAD/DEAH_box_helicase_dom.
IPR025313. DUF4217.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF13959. DUF4217. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-94.
    Tissue: Placenta.
  3. "Myc-Max heterodimers activate a DEAD box gene and interact with multiple E box-related sites in vivo."
    Grandori C., Mac J., Siebelt F., Ayer D.A., Eisenman R.E.
    EMBO J. 15:4344-4357(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 56-670.
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 61-670, VARIANT SER-94.
  5. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
    Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
    Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 370-376.
    Tissue: Platelet.
  6. "NOP132 is required for proper nucleolus localization of DEAD-box RNA helicase DDX47."
    Sekiguchi T., Hayano T., Yanagida M., Takahashi N., Nishimoto T.
    Nucleic Acids Res. 34:4593-4608(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NOL8, SUBCELLULAR LOCATION.
  7. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 149-387 IN COMPLEX WITH PHOSPHATE.
  10. Cited for: VARIANT [LARGE SCALE ANALYSIS] ARG-41.

Entry informationi

Entry nameiDDX18_HUMAN
AccessioniPrimary (citable) accession number: Q9NVP1
Secondary accession number(s): Q6GTZ9
, Q6IAU4, Q92732, Q9BQB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 10, 2002
Last modified: November 26, 2014
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3