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Q9NVP1 (DDX18_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent RNA helicase DDX18

EC=3.6.4.13
Alternative name(s):
DEAD box protein 18
Myc-regulated DEAD box protein
Short name=MrDb
Gene names
Name:DDX18
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length670 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable RNA-dependent helicase.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Interacts with NOL8; the interaction is RNA-dependent. Ref.6

Subcellular location

Nucleusnucleolus Ref.6.

Sequence similarities

Belongs to the DEAD box helicase family. DDX18/HAS1 subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Sequence caution

The sequence CAA67295.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 670670ATP-dependent RNA helicase DDX18
PRO_0000055001

Regions

Domain210 – 385176Helicase ATP-binding
Domain399 – 569171Helicase C-terminal
Nucleotide binding223 – 2308ATP Probable
Motif179 – 20729Q motif
Motif333 – 3364DEAD box
Compositional bias108 – 1169Poly-Lys
Compositional bias636 – 6427Poly-Gly

Natural variations

Natural variant411G → R in a breast cancer sample; somatic mutation. Ref.10
VAR_035841
Natural variant941T → S. Ref.2 Ref.4
Corresponds to variant rs1052637 [ dbSNP | Ensembl ].
VAR_013293
Natural variant6471K → R.
Corresponds to variant rs10179772 [ dbSNP | Ensembl ].
VAR_033857

Experimental info

Sequence conflict2481P → T in BAA91709. Ref.1
Sequence conflict3821E → G in CAG33341. Ref.4

Secondary structure

.................................... 670
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9NVP1 [UniParc].

Last modified May 10, 2002. Version 2.
Checksum: 2FE653A98751F9DC

FASTA67075,407
        10         20         30         40         50         60 
MSHLPMKLLR KKIEKRNLKL RQRNLKFQGA SNLTLSETQN GDVSEETMGS RKVKKSKQKP 

        70         80         90        100        110        120 
MNVGLSETQN GGMSQEAVGN IKVTKSPQKS TVLTNGEAAM QSSNSESKKK KKKKRKMVND 

       130        140        150        160        170        180 
AEPDTKKAKT ENKGKSEEES AETTKETENN VEKPDNDEDE SEVPSLPLGL TGAFEDTSFA 

       190        200        210        220        230        240 
SLCNLVNENT LKAIKEMGFT NMTEIQHKSI RPLLEGRDLL AAAKTGSGKT LAFLIPAVEL 

       250        260        270        280        290        300 
IVKLRFMPRN GTGVLILSPT RELAMQTFGV LKELMTHHVH TYGLIMGGSN RSAEAQKLGN 

       310        320        330        340        350        360 
GINIIVATPG RLLDHMQNTP GFMYKNLQCL VIDEADRILD VGFEEELKQI IKLLPTRRQT 

       370        380        390        400        410        420 
MLFSATQTRK VEDLARISLK KEPLYVGVDD DKANATVDGL EQGYVVCPSE KRFLLLFTFL 

       430        440        450        460        470        480 
KKNRKKKLMV FFSSCMSVKY HYELLNYIDL PVLAIHGKQK QNKRTTTFFQ FCNADSGTLL 

       490        500        510        520        530        540 
CTDVAARGLD IPEVDWIVQY DPPDDPKEYI HRVGRTARGL NGRGHALLIL RPEELGFLRY 

       550        560        570        580        590        600 
LKQSKVPLSE FDFSWSKISD IQSQLEKLIE KNYFLHKSAQ EAYKSYIRAY DSHSLKQIFN 

       610        620        630        640        650        660 
VNNLNLPQVA LSFGFKVPPF VDLNVNSNEG KQKKRGGGGG FGYQKTKKVE KSKIFKHISK 

       670 
KSSDSRQFSH 

« Hide

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-94.
Tissue: Placenta.
[3]"Myc-Max heterodimers activate a DEAD box gene and interact with multiple E box-related sites in vivo."
Grandori C., Mac J., Siebelt F., Ayer D.A., Eisenman R.E.
EMBO J. 15:4344-4357(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 56-670.
[4]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 61-670, VARIANT SER-94.
[5]"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 370-376.
Tissue: Platelet.
[6]"NOP132 is required for proper nucleolus localization of DEAD-box RNA helicase DDX47."
Sekiguchi T., Hayano T., Yanagida M., Takahashi N., Nishimoto T.
Nucleic Acids Res. 34:4593-4608(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NOL8, SUBCELLULAR LOCATION.
[7]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Comparative structural analysis of human DEAD-box RNA helicases."
Schutz P., Karlberg T., van den Berg S., Collins R., Lehtio L., Hogbom M., Holmberg-Schiavone L., Tempel W., Park H.W., Hammarstrom M., Moche M., Thorsell A.G., Schuler H.
PLoS ONE 5:E12791-E12791(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 149-387 IN COMPLEX WITH PHOSPHATE.
[10]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] ARG-41.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK001467 mRNA. Translation: BAA91709.1.
BC001238 mRNA. Translation: AAH01238.1.
BC003360 mRNA. Translation: AAH03360.1.
BC024739 mRNA. Translation: AAH24739.1.
X98743 mRNA. Translation: CAA67295.1. Different initiation.
CR457060 mRNA. Translation: CAG33341.1.
CCDSCCDS2120.1.
PIRS71758.
RefSeqNP_006764.3. NM_006773.3.
UniGeneHs.744922.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3LY5X-ray2.80A/B149-387[»]
ProteinModelPortalQ9NVP1.
SMRQ9NVP1. Positions 169-614.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114404. 24 interactions.
IntActQ9NVP1. 7 interactions.
MINTMINT-1382348.
STRING9606.ENSP00000263239.

PTM databases

PhosphoSiteQ9NVP1.

Polymorphism databases

DMDM20532388.

2D gel databases

SWISS-2DPAGEQ9NVP1.

Proteomic databases

MaxQBQ9NVP1.
PaxDbQ9NVP1.
PeptideAtlasQ9NVP1.
PRIDEQ9NVP1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000263239; ENSP00000263239; ENSG00000088205.
GeneID8886.
KEGGhsa:8886.
UCSCuc002tlh.1. human.

Organism-specific databases

CTD8886.
GeneCardsGC02P118572.
HGNCHGNC:2741. DDX18.
HPAHPA035400.
HPA041056.
MIM606355. gene.
neXtProtNX_Q9NVP1.
PharmGKBPA27207.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0513.
HOGENOMHOG000268799.
HOVERGENHBG105702.
InParanoidQ9NVP1.
KOK13179.
OMAKGFVFRN.
OrthoDBEOG7GFB4K.
PhylomeDBQ9NVP1.
TreeFamTF300471.

Gene expression databases

ArrayExpressQ9NVP1.
BgeeQ9NVP1.
CleanExHS_DDX18.
GenevestigatorQ9NVP1.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR025313. DUF4217.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF13959. DUF4217. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSDDX18. human.
EvolutionaryTraceQ9NVP1.
GenomeRNAi8886.
NextBio33369.
PROQ9NVP1.
SOURCESearch...

Entry information

Entry nameDDX18_HUMAN
AccessionPrimary (citable) accession number: Q9NVP1
Secondary accession number(s): Q6GTZ9 expand/collapse secondary AC list , Q6IAU4, Q92732, Q9BQB7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 10, 2002
Last modified: July 9, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM