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Protein

ATP-dependent RNA helicase DDX18

Gene

DDX18

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable RNA-dependent helicase.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi223 – 230ATPCurated8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: ProtInc
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000088205-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DDX18 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 18
Myc-regulated DEAD box protein
Short name:
MrDb
Gene namesi
Name:DDX18
Synonyms:cPERP-D1 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:2741. DDX18.

Subcellular locationi

GO - Cellular componenti

  • chromosome Source: UniProtKB
  • membrane Source: UniProtKB
  • nucleolus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi8886.
OpenTargetsiENSG00000088205.
PharmGKBiPA27207.

Polymorphism and mutation databases

BioMutaiDDX18.
DMDMi20532388.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000550011 – 670ATP-dependent RNA helicase DDX18Add BLAST670

Proteomic databases

EPDiQ9NVP1.
MaxQBiQ9NVP1.
PaxDbiQ9NVP1.
PeptideAtlasiQ9NVP1.
PRIDEiQ9NVP1.

2D gel databases

SWISS-2DPAGEQ9NVP1.

PTM databases

iPTMnetiQ9NVP1.
PhosphoSitePlusiQ9NVP1.
SwissPalmiQ9NVP1.

Expressioni

Gene expression databases

BgeeiENSG00000088205.
CleanExiHS_DDX18.
ExpressionAtlasiQ9NVP1. baseline and differential.
GenevisibleiQ9NVP1. HS.

Organism-specific databases

HPAiHPA035400.
HPA041056.

Interactioni

Subunit structurei

Interacts with NOL8; the interaction is RNA-dependent.2 Publications

Protein-protein interaction databases

BioGridi114404. 109 interactors.
IntActiQ9NVP1. 16 interactors.
MINTiMINT-1382348.
STRINGi9606.ENSP00000263239.

Structurei

Secondary structure

1670
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi175 – 177Combined sources3
Helixi188 – 196Combined sources9
Helixi204 – 215Combined sources12
Helixi229 – 243Combined sources15
Helixi248 – 250Combined sources3
Beta strandi254 – 257Combined sources4
Helixi261 – 274Combined sources14
Turni275 – 277Combined sources3
Beta strandi282 – 285Combined sources4
Beta strandi287 – 289Combined sources3
Helixi291 – 300Combined sources10
Beta strandi303 – 307Combined sources5
Helixi309 – 318Combined sources10
Beta strandi329 – 332Combined sources4
Helixi335 – 340Combined sources6
Helixi344 – 353Combined sources10
Beta strandi356 – 363Combined sources8
Helixi369 – 378Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LY5X-ray2.80A/B149-387[»]
ProteinModelPortaliQ9NVP1.
SMRiQ9NVP1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NVP1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini210 – 385Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST176
Domaini399 – 569Helicase C-terminalPROSITE-ProRule annotationAdd BLAST171

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi179 – 207Q motifAdd BLAST29
Motifi333 – 336DEAD box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi108 – 116Poly-Lys9
Compositional biasi636 – 642Poly-Gly7

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0342. Eukaryota.
COG0513. LUCA.
GeneTreeiENSGT00680000100037.
HOGENOMiHOG000268799.
HOVERGENiHBG105702.
InParanoidiQ9NVP1.
KOiK13179.
OMAiIQHKTIR.
OrthoDBiEOG091G06N7.
PhylomeDBiQ9NVP1.
TreeFamiTF300471.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR025313. DUF4217.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF13959. DUF4217. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM01178. DUF4217. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9NVP1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHLPMKLLR KKIEKRNLKL RQRNLKFQGA SNLTLSETQN GDVSEETMGS
60 70 80 90 100
RKVKKSKQKP MNVGLSETQN GGMSQEAVGN IKVTKSPQKS TVLTNGEAAM
110 120 130 140 150
QSSNSESKKK KKKKRKMVND AEPDTKKAKT ENKGKSEEES AETTKETENN
160 170 180 190 200
VEKPDNDEDE SEVPSLPLGL TGAFEDTSFA SLCNLVNENT LKAIKEMGFT
210 220 230 240 250
NMTEIQHKSI RPLLEGRDLL AAAKTGSGKT LAFLIPAVEL IVKLRFMPRN
260 270 280 290 300
GTGVLILSPT RELAMQTFGV LKELMTHHVH TYGLIMGGSN RSAEAQKLGN
310 320 330 340 350
GINIIVATPG RLLDHMQNTP GFMYKNLQCL VIDEADRILD VGFEEELKQI
360 370 380 390 400
IKLLPTRRQT MLFSATQTRK VEDLARISLK KEPLYVGVDD DKANATVDGL
410 420 430 440 450
EQGYVVCPSE KRFLLLFTFL KKNRKKKLMV FFSSCMSVKY HYELLNYIDL
460 470 480 490 500
PVLAIHGKQK QNKRTTTFFQ FCNADSGTLL CTDVAARGLD IPEVDWIVQY
510 520 530 540 550
DPPDDPKEYI HRVGRTARGL NGRGHALLIL RPEELGFLRY LKQSKVPLSE
560 570 580 590 600
FDFSWSKISD IQSQLEKLIE KNYFLHKSAQ EAYKSYIRAY DSHSLKQIFN
610 620 630 640 650
VNNLNLPQVA LSFGFKVPPF VDLNVNSNEG KQKKRGGGGG FGYQKTKKVE
660 670
KSKIFKHISK KSSDSRQFSH
Length:670
Mass (Da):75,407
Last modified:May 10, 2002 - v2
Checksum:i2FE653A98751F9DC
GO

Sequence cautioni

The sequence CAA67295 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti248P → T in BAA91709 (PubMed:14702039).Curated1
Sequence conflicti382E → G in CAG33341 (Ref. 4) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03584141G → R in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_01329394T → S.2 PublicationsCorresponds to variant rs1052637dbSNPEnsembl.1
Natural variantiVAR_033857647K → R.Corresponds to variant rs10179772dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001467 mRNA. Translation: BAA91709.1.
BC001238 mRNA. Translation: AAH01238.1.
BC003360 mRNA. Translation: AAH03360.1.
BC024739 mRNA. Translation: AAH24739.1.
X98743 mRNA. Translation: CAA67295.1. Different initiation.
CR457060 mRNA. Translation: CAG33341.1.
CCDSiCCDS2120.1.
PIRiS71758.
RefSeqiNP_006764.3. NM_006773.3.
UniGeneiHs.744922.

Genome annotation databases

EnsembliENST00000263239; ENSP00000263239; ENSG00000088205.
GeneIDi8886.
KEGGihsa:8886.
UCSCiuc002tlh.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001467 mRNA. Translation: BAA91709.1.
BC001238 mRNA. Translation: AAH01238.1.
BC003360 mRNA. Translation: AAH03360.1.
BC024739 mRNA. Translation: AAH24739.1.
X98743 mRNA. Translation: CAA67295.1. Different initiation.
CR457060 mRNA. Translation: CAG33341.1.
CCDSiCCDS2120.1.
PIRiS71758.
RefSeqiNP_006764.3. NM_006773.3.
UniGeneiHs.744922.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LY5X-ray2.80A/B149-387[»]
ProteinModelPortaliQ9NVP1.
SMRiQ9NVP1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114404. 109 interactors.
IntActiQ9NVP1. 16 interactors.
MINTiMINT-1382348.
STRINGi9606.ENSP00000263239.

PTM databases

iPTMnetiQ9NVP1.
PhosphoSitePlusiQ9NVP1.
SwissPalmiQ9NVP1.

Polymorphism and mutation databases

BioMutaiDDX18.
DMDMi20532388.

2D gel databases

SWISS-2DPAGEQ9NVP1.

Proteomic databases

EPDiQ9NVP1.
MaxQBiQ9NVP1.
PaxDbiQ9NVP1.
PeptideAtlasiQ9NVP1.
PRIDEiQ9NVP1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263239; ENSP00000263239; ENSG00000088205.
GeneIDi8886.
KEGGihsa:8886.
UCSCiuc002tlh.2. human.

Organism-specific databases

CTDi8886.
DisGeNETi8886.
GeneCardsiDDX18.
HGNCiHGNC:2741. DDX18.
HPAiHPA035400.
HPA041056.
MIMi606355. gene.
neXtProtiNX_Q9NVP1.
OpenTargetsiENSG00000088205.
PharmGKBiPA27207.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0342. Eukaryota.
COG0513. LUCA.
GeneTreeiENSGT00680000100037.
HOGENOMiHOG000268799.
HOVERGENiHBG105702.
InParanoidiQ9NVP1.
KOiK13179.
OMAiIQHKTIR.
OrthoDBiEOG091G06N7.
PhylomeDBiQ9NVP1.
TreeFamiTF300471.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000088205-MONOMER.

Miscellaneous databases

ChiTaRSiDDX18. human.
EvolutionaryTraceiQ9NVP1.
GenomeRNAii8886.
PROiQ9NVP1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000088205.
CleanExiHS_DDX18.
ExpressionAtlasiQ9NVP1. baseline and differential.
GenevisibleiQ9NVP1. HS.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR025313. DUF4217.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF13959. DUF4217. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM01178. DUF4217. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDDX18_HUMAN
AccessioniPrimary (citable) accession number: Q9NVP1
Secondary accession number(s): Q6GTZ9
, Q6IAU4, Q92732, Q9BQB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 10, 2002
Last modified: November 2, 2016
This is version 153 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.