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Protein

Guanine nucleotide-binding protein-like 3-like protein

Gene

GNL3L

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Stabilizes TERF1 telomeric association by preventing TERF1 recruitment by PML. Stabilizes TERF1 protein by preventing its ubiquitination and hence proteasomal degradation. Does so by interfering with TERF1-binding to FBXO4 E3 ubiquitin-protein ligase. Required for cell proliferation. By stabilizing TRF1 protein during mitosis, promotes metaphase-to-anaphase transition. Stabilizes MDM2 protein by preventing its ubiquitination, and hence proteasomal degradation. By acting on MDM2, may affect TP53 activity. Required for normal processing of ribosomal pre-rRNA. Binds GTP.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi173 – 176GTPSequence analysis4
Nucleotide bindingi259 – 266GTPSequence analysis8
Nucleotide bindingi303 – 306GTPSequence analysis4

GO - Molecular functioni

GO - Biological processi

  • negative regulation of protein binding Source: BHF-UCL
  • negative regulation of protein sumoylation Source: BHF-UCL
  • negative regulation of protein ubiquitination Source: BHF-UCL
  • negative regulation of telomere maintenance via telomerase Source: BHF-UCL
  • positive regulation of protein homodimerization activity Source: BHF-UCL
  • positive regulation of protein localization to chromosome, telomeric region Source: BHF-UCL
  • regulation of protein stability Source: BHF-UCL
  • ribosome biogenesis Source: GO_Central

Keywordsi

Biological processRibosome biogenesis
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein-like 3-like protein
Gene namesi
Name:GNL3L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

EuPathDBiHostDB:ENSG00000130119.15
HGNCiHGNC:25553 GNL3L
MIMi300873 gene
neXtProtiNX_Q9NVN8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi9 – 10KK → AA: Loss of nucleolar localization; when associated with 34-A-A-35. Loss of nuclear location; when associated with 19-A-A-20. 1 Publication2
Mutagenesisi19 – 20KK → AA: Loss of nuclear location; when associated with 9-A-A-10. Loss of nuclear location; when associated with 34-A-A-35. 1 Publication2
Mutagenesisi34 – 35KK → AA: Loss of nucleolar localization; when associated with 9-A-A-10. Loss of nuclear location; when associated with 19-A-A-20. 1 Publication2
Mutagenesisi145 – 147RDP → AAA: Loss of GTP binding. Loss of nucleolar localization. No effect on nuclear localization. 1 Publication3
Mutagenesisi309 – 310PG → AA: Loss of nucleolar localization. No effect on nuclear localization. 1 Publication2

Organism-specific databases

DisGeNETi54552
OpenTargetsiENSG00000130119
PharmGKBiPA134922876

Polymorphism and mutation databases

BioMutaiGNL3L
DMDMi74752999

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002843811 – 582Guanine nucleotide-binding protein-like 3-like proteinAdd BLAST582

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki477Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ9NVN8
MaxQBiQ9NVN8
PaxDbiQ9NVN8
PeptideAtlasiQ9NVN8
PRIDEiQ9NVN8
ProteomicsDBi82836

PTM databases

iPTMnetiQ9NVN8
PhosphoSitePlusiQ9NVN8

Expressioni

Developmental stagei

Up-regulated during mitosis and down-regulated in the G1 phase.1 Publication

Gene expression databases

BgeeiENSG00000130119
CleanExiHS_GNL3L
ExpressionAtlasiQ9NVN8 baseline and differential
GenevisibleiQ9NVN8 HS

Organism-specific databases

HPAiHPA036314
HPA036315

Interactioni

Subunit structurei

Interacts with MDM2; this interaction, which occurs in the nucleoplasm, stabilizes MDM2. Indirectly interacts with TP53, via MDM2-binding. Interacts with TERF1; this interaction probably occurs in the nucleoplasm and is increased during mitosis, when the nucleolus is disassembled. This binding may promote TERF1 homodimerization. Interacts with TERT.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi120037, 45 interactors
IntActiQ9NVN8, 25 interactors
MINTiQ9NVN8
STRINGi9606.ENSP00000338573

Structurei

3D structure databases

ProteinModelPortaliQ9NVN8
SMRiQ9NVN8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini125 – 310CP-type GPROSITE-ProRule annotationAdd BLAST186

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni9 – 35Required for nucleolar localizationAdd BLAST27

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili58 – 88Sequence analysisAdd BLAST31

Domaini

In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.

Sequence similaritiesi

Belongs to the TRAFAC class YlqF/YawG GTPase family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2484 Eukaryota
COG1161 LUCA
GeneTreeiENSGT00810000125472
HOGENOMiHOG000207716
HOVERGENiHBG051747
InParanoidiQ9NVN8
KOiK14538
OMAiHQMGWAK
OrthoDBiEOG091G08IZ
PhylomeDBiQ9NVN8
TreeFamiTF313085

Family and domain databases

Gene3Di1.10.1580.10, 1 hit
InterProiView protein in InterPro
IPR030378 G_CP_dom
IPR023179 GTP-bd_ortho_bundle_sf
IPR006073 GTP_binding_domain
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF01926 MMR_HSR1, 1 hit
PRINTSiPR00326 GTP1OBG
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51721 G_CP, 1 hit

Sequencei

Sequence statusi: Complete.

Q9NVN8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMKLRHKNKK PGEGSKGHKK ISWPYPQPAK QNGKKATSKV PSAPHFVHPN
60 70 80 90 100
DHANREAELK KKWVEEMREK QQAAREQERQ KRRTIESYCQ DVLRRQEEFE
110 120 130 140 150
HKEEVLQELN MFPQLDDEAT RKAYYKEFRK VVEYSDVILE VLDARDPLGC
160 170 180 190 200
RCFQMEEAVL RAQGNKKLVL VLNKIDLVPK EVVEKWLDYL RNELPTVAFK
210 220 230 240 250
ASTQHQVKNL NRCSVPVDQA SESLLKSKAC FGAENLMRVL GNYCRLGEVR
260 270 280 290 300
THIRVGVVGL PNVGKSSLIN SLKRSRACSV GAVPGITKFM QEVYLDKFIR
310 320 330 340 350
LLDAPGIVPG PNSEVGTILR NCVHVQKLAD PVTPVETILQ RCNLEEISNY
360 370 380 390 400
YGVSGFQTTE HFLTAVAHRL GKKKKGGLYS QEQAAKAVLA DWVSGKISFY
410 420 430 440 450
IPPPATHTLP THLSAEIVKE MTEVFDIEDT EQANEDTMEC LATGESDELL
460 470 480 490 500
GDTDPLEMEI KLLHSPMTKI ADAIENKTTV YKIGDLTGYC TNPNRHQMGW
510 520 530 540 550
AKRNVDHRPK SNSMVDVCSV DRRSVLQRIM ETDPLQQGQA LASALKNKKK
560 570 580
MQKRADKIAS KLSDSMMSAL DLSGNADDGV GD
Length:582
Mass (Da):65,573
Last modified:October 1, 2000 - v1
Checksum:iB65EE072424F54BC
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049495320R → H. Corresponds to variant dbSNP:rs2298284Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001475 mRNA Translation: BAA91712.1
AL391139 Genomic DNA No translation available.
BC011720 mRNA Translation: AAH11720.1
CCDSiCCDS14360.1
RefSeqiNP_001171748.1, NM_001184819.1
NP_061940.1, NM_019067.5
UniGeneiHs.654677

Genome annotation databases

EnsembliENST00000336470; ENSP00000338573; ENSG00000130119
ENST00000360845; ENSP00000354091; ENSG00000130119
GeneIDi54552
KEGGihsa:54552
UCSCiuc004dth.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiGNL3L_HUMAN
AccessioniPrimary (citable) accession number: Q9NVN8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: October 1, 2000
Last modified: June 20, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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