ID   RIC8B_HUMAN             Reviewed;         520 AA.
AC   Q9NVN3; A2RTZ0; Q4G103; Q6ZRN4; Q86WD3;
DT   16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2010, sequence version 2.
DT   24-JAN-2024, entry version 157.
DE   RecName: Full=Synembryn-B;
DE   AltName: Full=Brain synembryn;
DE            Short=hSyn;
DE   AltName: Full=Protein Ric-8B;
GN   Name=RIC8B;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
RC   TISSUE=Teratocarcinoma, and Testis;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16541075; DOI=10.1038/nature04569;
RA   Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y.,
RA   Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C.,
RA   Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C.,
RA   Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R.,
RA   Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E.,
RA   Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y.,
RA   Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G.,
RA   Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H.,
RA   Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S.,
RA   Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M.,
RA   Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H.,
RA   Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q.,
RA   Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V.,
RA   Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E.,
RA   Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K.,
RA   Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D.,
RA   Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R.,
RA   David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E.,
RA   D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N.,
RA   Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N.,
RA   Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R.,
RA   Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S.,
RA   LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H.,
RA   Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P.,
RA   Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G.,
RA   Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E.,
RA   Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S.,
RA   Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O.,
RA   Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J.,
RA   Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A.,
RA   Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M.,
RA   Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I.,
RA   Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A.,
RA   Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y.,
RA   Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A.,
RA   Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F.,
RA   Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L.,
RA   Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G.,
RA   Gibbs R.A.;
RT   "The finished DNA sequence of human chromosome 12.";
RL   Nature 440:346-351(2006).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 5).
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 23-483 (ISOFORM 1), SUBCELLULAR LOCATION, AND
RP   INTERACTION WITH GNAS AND GNAQ.
RC   TISSUE=Brain;
RX   PubMed=12652642; DOI=10.1002/jcp.10300;
RA   Klattenhoff C., Montecino M., Soto X., Guzman L., Romo X., Garcia M.A.,
RA   Mellstrom B., Naranjo J.R., Hinrichs M.V., Olate J.;
RT   "Human brain synembryn interacts with Gsalpha and Gqalpha and is
RT   translocated to the plasma membrane in response to isoproterenol and
RT   carbachol.";
RL   J. Cell. Physiol. 195:151-157(2003).
RN   [5]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-468 AND THR-473, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Leukemic T-cell;
RX   PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA   Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA   Rodionov V., Han D.K.;
RT   "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT   reveals system-wide modulation of protein-protein interactions.";
RL   Sci. Signal. 2:RA46-RA46(2009).
CC   -!- FUNCTION: Guanine nucleotide exchange factor (GEF), which can activate
CC       some, but not all, G-alpha proteins by exchanging bound GDP for free
CC       GTP. Able to potentiate G(olf)-alpha-dependent cAMP accumulation
CC       suggesting that it may be an important component for odorant signal
CC       transduction. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with GDP-bound G alpha proteins GNAI1, GNAL, GNAS
CC       and GNAQ. Does not interact with G-alpha proteins when they are in
CC       complex with subunits beta and gamma. {ECO:0000269|PubMed:12652642}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cell cortex
CC       {ECO:0000269|PubMed:12652642}. Note=Localizes to the cell cortex.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=4;
CC       Name=5;
CC         IsoId=Q9NVN3-5; Sequence=Displayed;
CC       Name=1;
CC         IsoId=Q9NVN3-1; Sequence=VSP_039866;
CC       Name=3;
CC         IsoId=Q9NVN3-3; Sequence=VSP_018511, VSP_039866;
CC       Name=4;
CC         IsoId=Q9NVN3-4; Sequence=VSP_018510, VSP_018512, VSP_018513;
CC   -!- MISCELLANEOUS: [Isoform 1]: May be produced at very low levels due to a
CC       premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC       decay. {ECO:0000305}.
CC   -!- MISCELLANEOUS: [Isoform 4]: May be produced at very low levels due to a
CC       premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC       decay. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the synembryn family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAO65923.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
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DR   EMBL; AK001482; BAA91717.1; -; mRNA.
DR   EMBL; AK128102; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; AC007695; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC079385; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC090011; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC034220; AAH34220.1; -; mRNA.
DR   EMBL; BC132689; AAI32690.1; -; mRNA.
DR   EMBL; BC144056; AAI44057.1; -; mRNA.
DR   EMBL; AY221116; AAO65923.1; ALT_SEQ; mRNA.
DR   CCDS; CCDS81733.1; -. [Q9NVN3-1]
DR   CCDS; CCDS86331.1; -. [Q9NVN3-3]
DR   CCDS; CCDS9109.2; -. [Q9NVN3-5]
DR   RefSeq; NP_001317074.1; NM_001330145.1.
DR   RefSeq; NP_001317075.1; NM_001330146.1.
DR   RefSeq; NP_001317076.1; NM_001330147.1. [Q9NVN3-1]
DR   RefSeq; NP_060627.2; NM_018157.3. [Q9NVN3-5]
DR   AlphaFoldDB; Q9NVN3; -.
DR   SMR; Q9NVN3; -.
DR   BioGRID; 120486; 48.
DR   IntAct; Q9NVN3; 10.
DR   STRING; 9606.ENSP00000376582; -.
DR   iPTMnet; Q9NVN3; -.
DR   PhosphoSitePlus; Q9NVN3; -.
DR   BioMuta; RIC8B; -.
DR   DMDM; 308153488; -.
DR   EPD; Q9NVN3; -.
DR   jPOST; Q9NVN3; -.
DR   MassIVE; Q9NVN3; -.
DR   MaxQB; Q9NVN3; -.
DR   PaxDb; 9606-ENSP00000376583; -.
DR   PeptideAtlas; Q9NVN3; -.
DR   ProteomicsDB; 82832; -. [Q9NVN3-5]
DR   ProteomicsDB; 82833; -. [Q9NVN3-1]
DR   ProteomicsDB; 82834; -. [Q9NVN3-3]
DR   ProteomicsDB; 82835; -. [Q9NVN3-4]
DR   Pumba; Q9NVN3; -.
DR   Antibodypedia; 45122; 63 antibodies from 18 providers.
DR   DNASU; 55188; -.
DR   Ensembl; ENST00000355478.6; ENSP00000347662.2; ENSG00000111785.21. [Q9NVN3-3]
DR   Ensembl; ENST00000392839.6; ENSP00000376583.2; ENSG00000111785.21. [Q9NVN3-5]
DR   Ensembl; ENST00000462949.5; ENSP00000435301.1; ENSG00000111785.21. [Q9NVN3-1]
DR   Ensembl; ENST00000470960.1; ENSP00000436359.1; ENSG00000111785.21. [Q9NVN3-4]
DR   GeneID; 55188; -.
DR   KEGG; hsa:55188; -.
DR   UCSC; uc001tlx.4; human. [Q9NVN3-5]
DR   AGR; HGNC:25555; -.
DR   CTD; 55188; -.
DR   DisGeNET; 55188; -.
DR   GeneCards; RIC8B; -.
DR   HGNC; HGNC:25555; RIC8B.
DR   HPA; ENSG00000111785; Low tissue specificity.
DR   MIM; 609147; gene.
DR   neXtProt; NX_Q9NVN3; -.
DR   OpenTargets; ENSG00000111785; -.
DR   PharmGKB; PA142671064; -.
DR   VEuPathDB; HostDB:ENSG00000111785; -.
DR   eggNOG; KOG4464; Eukaryota.
DR   GeneTree; ENSGT00390000014700; -.
DR   HOGENOM; CLU_018602_1_0_1; -.
DR   InParanoid; Q9NVN3; -.
DR   OrthoDB; 5357832at2759; -.
DR   PhylomeDB; Q9NVN3; -.
DR   TreeFam; TF314907; -.
DR   PathwayCommons; Q9NVN3; -.
DR   SignaLink; Q9NVN3; -.
DR   BioGRID-ORCS; 55188; 10 hits in 1154 CRISPR screens.
DR   ChiTaRS; RIC8B; human.
DR   GenomeRNAi; 55188; -.
DR   Pharos; Q9NVN3; Tbio.
DR   PRO; PR:Q9NVN3; -.
DR   Proteomes; UP000005640; Chromosome 12.
DR   RNAct; Q9NVN3; Protein.
DR   Bgee; ENSG00000111785; Expressed in right uterine tube and 182 other cell types or tissues.
DR   ExpressionAtlas; Q9NVN3; baseline and differential.
DR   GO; GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
DR   GO; GO:0005813; C:centrosome; IDA:HPA.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005829; C:cytosol; IDA:HPA.
DR   GO; GO:0005886; C:plasma membrane; IDA:HPA.
DR   GO; GO:0001965; F:G-protein alpha-subunit binding; IDA:MGI.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR   GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR   GO; GO:0008277; P:regulation of G protein-coupled receptor signaling pathway; IDA:MGI.
DR   Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR019318; Gua_nucleotide_exch_fac_Ric8.
DR   InterPro; IPR008376; Synembryn.
DR   PANTHER; PTHR12425; SYNEMBRYN; 1.
DR   PANTHER; PTHR12425:SF2; SYNEMBRYN-B; 1.
DR   Pfam; PF10165; Ric8; 1.
DR   PRINTS; PR01802; SYNEMBRYN.
DR   SUPFAM; SSF48371; ARM repeat; 1.
DR   Genevisible; Q9NVN3; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cytoplasm; Guanine-nucleotide releasing factor;
KW   Phosphoprotein; Reference proteome.
FT   CHAIN           1..520
FT                   /note="Synembryn-B"
FT                   /id="PRO_0000235899"
FT   REGION          468..494
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        475..491
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         468
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:19690332"
FT   MOD_RES         473
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0007744|PubMed:19690332"
FT   VAR_SEQ         1..240
FT                   /note="Missing (in isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_018510"
FT   VAR_SEQ         1..44
FT                   /note="MDEERALYIVRAGEAGAIERVLRDYSDKHRATFKFESTDEDKRK -> MLAS
FT                   (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_018511"
FT   VAR_SEQ         241..247
FT                   /note="SWKVHKE -> MLSKLEK (in isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_018512"
FT   VAR_SEQ         484..520
FT                   /note="KEELLKPMGLKPDGTITPLEEALNQYSVIEETSSDTD -> NINLITGHLEE
FT                   PMPNPIDEMTEEQKEYEAMKLVNMLDKLSRRADVKDLHQDGG (in isoform 1
FT                   and isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:12652642,
FT                   ECO:0000303|PubMed:14702039, ECO:0000303|PubMed:15489334"
FT                   /id="VSP_039866"
FT   VAR_SEQ         484..520
FT                   /note="KEELLKPMGLKPDGTITPLEEALNQYSVIEETSSDTD -> NSIEFEERSHL
FT                   SRGK (in isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_018513"
FT   CONFLICT        201
FT                   /note="S -> T (in Ref. 3; AAO65923)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   520 AA;  58825 MW;  C4D6B01725E6FB22 CRC64;
     MDEERALYIV RAGEAGAIER VLRDYSDKHR ATFKFESTDE DKRKKLCEGI FKVLIKDIPT
     TCQVSCLEVL RILSRDKKVL VPVTTKENMQ ILLRLAKLNE LDDSLEKVSE FPVIVESLKC
     LCNIVFNSQM AQQLSLELNL AAKLCNLLRK CKDRKFINDI KCFDLRLLFL LSLLHTDIRS
     QLRYELQGLP LLTQILESAF SIKWTDEYES AIDHNGPPLS PQETDCAIEA LKALFNVTVD
     SWKVHKESDS HQFRVMAAVL RHCLLIVGPT EDKTEELHSN AVNLLSNVPV SCLDVLICPL
     THEETAQEAT TLDELPSNKT AEKETVLKNN TMVYNGMNME AIHVLLNFME KRIDKGSSYR
     EGLTPVLSLL TECSRAHRNI RKFLKDQVLP PLRDVTNRPE VGSTVRNKLV RLMTHVDLGV
     KQIAAEFLFV LCKERVDSLL KYTGYGNAAG LLAARGLLAG GRGDNWYSED EDTDTEEYKN
     AKPKEELLKP MGLKPDGTIT PLEEALNQYS VIEETSSDTD
//