Q9NVN3 (RIC8B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Synembryn-B

Alternative name(s):
Brain synembrin
Short name=hSyn
Protein Ric-8B

Gene names

Name:

RIC8B

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP By similarity. Able to potentiate G(olf)-alpha-dependent cAMP accumulation suggesting that it may be an important component for odorant signal transduction By similarity.
Subunit structure	Interacts with GDP-bound G alpha proteins GNAI1, GNAL, GNAS and GNAQ. Does not interact with G-alpha proteins when they are in complex with subunits beta and gamma. Ref.4
Subcellular location	Cytoplasm › cell cortex. Note: Localizes to the cell cortex. Ref.4
Sequence similarities	Belongs to the synembryn family.
Sequence caution	The sequence AAO65923.1 differs from that shown. Reason: Intron retention.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Molecular function	Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of G-protein coupled receptor protein signaling pathway Inferred from direct assay Ref.4. Source: MGI
Cellular_component	cell cortex Inferred from electronic annotation. Source: UniProtKB-SubCell centrosome Inferred from direct assay. Source: HPA cytosol Inferred from direct assay Ref.4. Source: MGI plasma membrane Inferred from direct assay Ref.4. Source: MGI
Molecular_function	G-protein alpha-subunit binding Inferred from direct assay Ref.4. Source: MGI guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 5 (identifier: Q9NVN3-5)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: Q9NVN3-1)

The sequence of this isoform differs from the canonical sequence as follows:
     484-520: KEELLKPMGL...VIEETSSDTD → NINLITGHLE...DVKDLHQDGG

Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Isoform 3 (identifier: Q9NVN3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: MDEERALYIVRAGEAGAIERVLRDYSDKHRATFKFESTDEDKRK → MLAS
     484-520: KEELLKPMGL...VIEETSSDTD → NINLITGHLE...DVKDLHQDGG

Note: No experimental confirmation available.

Isoform 4 (identifier: Q9NVN3-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-240: Missing.
     241-247: SWKVHKE → MLSKLEK
     484-520: KEELLKPMGLKPDGTITPLEEALNQYSVIEETSSDTD → NSIEFEERSHLSRGK

Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 520	520	Synembryn-B		PRO_0000235899
Amino acid modifications
	Modified residue	468	1	Phosphoserine Ref.5
	Modified residue	473	1	Phosphothreonine Ref.5
Natural variations
	Alternative sequence	1 – 240	240	Missing in isoform 4.		VSP_018510
	Alternative sequence	1 – 44	44	MDEER…EDKRK → MLAS in isoform 3.		VSP_018511
	Alternative sequence	241 – 247	7	SWKVHKE → MLSKLEK in isoform 4.		VSP_018512
	Alternative sequence	484 – 520	37	KEELL…SSDTD → NINLITGHLEEPMPNPIDEM TEEQKEYEAMKLVNMLDKLS RRADVKDLHQDGG in isoform 1 and isoform 3.		VSP_039866
	Alternative sequence	484 – 520	37	KEELL…SSDTD → NSIEFEERSHLSRGK in isoform 4.		VSP_018513
Experimental info
	Sequence conflict	201	1	S → T in AAO65923. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 5 [UniParc]. Last modified October 5, 2010. Version 2. Checksum: C4D6B01725E6FB22	FASTA	520	58,825
	10 20 30 40 50 60 MDEERALYIV RAGEAGAIER VLRDYSDKHR ATFKFESTDE DKRKKLCEGI FKVLIKDIPT 70 80 90 100 110 120 TCQVSCLEVL RILSRDKKVL VPVTTKENMQ ILLRLAKLNE LDDSLEKVSE FPVIVESLKC 130 140 150 160 170 180 LCNIVFNSQM AQQLSLELNL AAKLCNLLRK CKDRKFINDI KCFDLRLLFL LSLLHTDIRS 190 200 210 220 230 240 QLRYELQGLP LLTQILESAF SIKWTDEYES AIDHNGPPLS PQETDCAIEA LKALFNVTVD 250 260 270 280 290 300 SWKVHKESDS HQFRVMAAVL RHCLLIVGPT EDKTEELHSN AVNLLSNVPV SCLDVLICPL 310 320 330 340 350 360 THEETAQEAT TLDELPSNKT AEKETVLKNN TMVYNGMNME AIHVLLNFME KRIDKGSSYR 370 380 390 400 410 420 EGLTPVLSLL TECSRAHRNI RKFLKDQVLP PLRDVTNRPE VGSTVRNKLV RLMTHVDLGV 430 440 450 460 470 480 KQIAAEFLFV LCKERVDSLL KYTGYGNAAG LLAARGLLAG GRGDNWYSED EDTDTEEYKN 490 500 510 520 AKPKEELLKP MGLKPDGTIT PLEEALNQYS VIEETSSDTD
	Isoform 1 [UniParc]. Checksum: 33630BB5FC93B65A	FASTA	536	60,840
	10 20 30 40 50 60 MDEERALYIV RAGEAGAIER VLRDYSDKHR ATFKFESTDE DKRKKLCEGI FKVLIKDIPT 70 80 90 100 110 120 TCQVSCLEVL RILSRDKKVL VPVTTKENMQ ILLRLAKLNE LDDSLEKVSE FPVIVESLKC 130 140 150 160 170 180 LCNIVFNSQM AQQLSLELNL AAKLCNLLRK CKDRKFINDI KCFDLRLLFL LSLLHTDIRS 190 200 210 220 230 240 QLRYELQGLP LLTQILESAF SIKWTDEYES AIDHNGPPLS PQETDCAIEA LKALFNVTVD 250 260 270 280 290 300 SWKVHKESDS HQFRVMAAVL RHCLLIVGPT EDKTEELHSN AVNLLSNVPV SCLDVLICPL 310 320 330 340 350 360 THEETAQEAT TLDELPSNKT AEKETVLKNN TMVYNGMNME AIHVLLNFME KRIDKGSSYR 370 380 390 400 410 420 EGLTPVLSLL TECSRAHRNI RKFLKDQVLP PLRDVTNRPE VGSTVRNKLV RLMTHVDLGV 430 440 450 460 470 480 KQIAAEFLFV LCKERVDSLL KYTGYGNAAG LLAARGLLAG GRGDNWYSED EDTDTEEYKN 490 500 510 520 530 AKPNINLITG HLEEPMPNPI DEMTEEQKEY EAMKLVNMLD KLSRRADVKD LHQDGG
	Isoform 3 [UniParc]. Checksum: 43D03EB653E815DC	FASTA	496	56,057
	10 20 30 40 50 60 MLASKLCEGI FKVLIKDIPT TCQVSCLEVL RILSRDKKVL VPVTTKENMQ ILLRLAKLNE 70 80 90 100 110 120 LDDSLEKVSE FPVIVESLKC LCNIVFNSQM AQQLSLELNL AAKLCNLLRK CKDRKFINDI 130 140 150 160 170 180 KCFDLRLLFL LSLLHTDIRS QLRYELQGLP LLTQILESAF SIKWTDEYES AIDHNGPPLS 190 200 210 220 230 240 PQETDCAIEA LKALFNVTVD SWKVHKESDS HQFRVMAAVL RHCLLIVGPT EDKTEELHSN 250 260 270 280 290 300 AVNLLSNVPV SCLDVLICPL THEETAQEAT TLDELPSNKT AEKETVLKNN TMVYNGMNME 310 320 330 340 350 360 AIHVLLNFME KRIDKGSSYR EGLTPVLSLL TECSRAHRNI RKFLKDQVLP PLRDVTNRPE 370 380 390 400 410 420 VGSTVRNKLV RLMTHVDLGV KQIAAEFLFV LCKERVDSLL KYTGYGNAAG LLAARGLLAG 430 440 450 460 470 480 GRGDNWYSED EDTDTEEYKN AKPNINLITG HLEEPMPNPI DEMTEEQKEY EAMKLVNMLD 490 KLSRRADVKD LHQDGG
	Isoform 4 [UniParc]. Checksum: 076D3D9809C4FE30	FASTA	258	28,953
	10 20 30 40 50 60 MLSKLEKSDS HQFRVMAAVL RHCLLIVGPT EDKTEELHSN AVNLLSNVPV SCLDVLICPL 70 80 90 100 110 120 THEETAQEAT TLDELPSNKT AEKETVLKNN TMVYNGMNME AIHVLLNFME KRIDKGSSYR 130 140 150 160 170 180 EGLTPVLSLL TECSRAHRNI RKFLKDQVLP PLRDVTNRPE VGSTVRNKLV RLMTHVDLGV 190 200 210 220 230 240 KQIAAEFLFV LCKERVDSLL KYTGYGNAAG LLAARGLLAG GRGDNWYSED EDTDTEEYKN 250 AKPNSIEFEE RSHLSRGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4). Tissue: Teratocarcinoma and Testis.
[2]	"The finished DNA sequence of human chromosome 12." Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A. Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 5). Tissue: Brain.
[4]	"Human brain synembryn interacts with Gsalpha and Gqalpha and is translocated to the plasma membrane in response to isoproterenol and carbachol." Klattenhoff C., Montecino M., Soto X., Guzman L., Romo X., Garcia M.A., Mellstrom B., Naranjo J.R., Hinrichs M.V., Olate J. J. Cell. Physiol. 195:151-157(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 23-483 (ISOFORM 1), SUBCELLULAR LOCATION, INTERACTION WITH GNAS AND GNAQ. Tissue: Brain.
[5]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-468 AND THR-473, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK001482 mRNA. Translation: BAA91717.1. AK128102 mRNA. No translation available. AC007695 Genomic DNA. No translation available. AC079385 Genomic DNA. No translation available. AC090011 Genomic DNA. No translation available. BC034220 mRNA. Translation: AAH34220.1. BC132689 mRNA. Translation: AAI32690.1. BC144056 mRNA. Translation: AAI44057.1. AY221116 mRNA. Translation: AAO65923.1. Sequence problems.
IPI	IPI00018771. IPI00443951. IPI00746112. IPI00853186.
RefSeq	NP_060627.2. NM_018157.2.
UniGene	Hs.131306.
3D structure databases
ProteinModelPortal	Q9NVN3.
SMR	Q9NVN3. Positions 409-434.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000376583.
PTM databases
PhosphoSite	Q9NVN3.
Polymorphism databases
DMDM	308153488.
Proteomic databases
PaxDb	Q9NVN3.
PRIDE	Q9NVN3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000355478; ENSP00000347662; ENSG00000111785. ENST00000392839; ENSP00000376583; ENSG00000111785. ENST00000462949; ENSP00000435301; ENSG00000111785. ENST00000470960; ENSP00000436359; ENSG00000111785.
GeneID	55188.
KEGG	hsa:55188.
UCSC	uc001tlw.3. human. uc001tlx.3. human. uc001tly.3. human.
Organism-specific databases
CTD	55188.
GeneCards	GC12P107168.
HGNC	HGNC:25555. RIC8B.
HPA	HPA042746.
MIM	609147. gene.
neXtProt	NX_Q9NVN3.
PharmGKB	PA142671064.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG322219.
HOGENOM	HOG000236345.
HOVERGEN	HBG054867.
Gene expression databases
ArrayExpress	Q9NVN3.
Bgee	Q9NVN3.
CleanEx	HS_RIC8B.
Genevestigator	Q9NVN3.
GermOnline	ENSG00000111785. Homo sapiens.
Family and domain databases
Gene3D	1.25.10.10. 1 hit.
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR019318. Gua_nucleotide_exch_fac_Ric8. IPR008376. Synembryn. [Graphical view]
Pfam	PF10165. Ric8. 1 hit. [Graphical view]
PRINTS	PR01802. SYNEMBRYN.
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	55188.
NextBio	59035.
SOURCE	Search...

Entry information

Entry name

RIC8B_HUMAN

Accession

Primary (citable) accession number: Q9NVN3
Secondary accession number(s): A2RTZ0 , Q4G103, Q6ZRN4, Q86WD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	October 5, 2010
Last modified:	May 1, 2013
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.