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Protein

Integrator complex subunit 13

Gene

INTS13

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Crucial regulator of the mitotic cell cycle and development. At prophase, required for dynein anchoring to the nuclear envelope important for proper centrosome-nucleus coupling. At G2/M phase, may be required for proper spindle formation and execution of cytokinesis. Probable component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing (PubMed:23904267).2 Publications3 Publications

Miscellaneous

RNAi in HeLa cells results in a multinucleated phenotype.

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • centrosome localization Source: MGI
  • flagellated sperm motility Source: UniProtKB
  • mitotic spindle organization Source: MGI
  • protein localization to nuclear envelope Source: MGI
  • regulation of fertilization Source: UniProtKB
  • regulation of mitotic cell cycle Source: UniProtKB
  • snRNA transcription from RNA polymerase II promoter Source: Reactome

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-HSA-6807505. RNA polymerase II transcribes snRNA genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Integrator complex subunit 13Imported
Alternative name(s):
Cell cycle regulator Mat89Bb homolog
Germ cell tumor 1
Protein asunder homolog
Sarcoma antigen NY-SAR-95
Gene namesi
Name:INTS13Imported
Synonyms:ASUNImported, C12orf11, GCT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000064102.14.
HGNCiHGNC:20174. INTS13.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi577 – 582REDKED → AAAAAA: Loss of nuclear location. Location is mainly cytoplasmic or diffuse. Loss of Dynein recruitment to nuclear envelope. 1 Publication6

Organism-specific databases

DisGeNETi55726.
OpenTargetsiENSG00000064102.
PharmGKBiPA134892469.

Polymorphism and mutation databases

BioMutaiASUN.
DMDMi71153010.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000898451 – 706Integrator complex subunit 13Add BLAST706

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki611Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei623PhosphoserineCombined sources1
Modified residuei626PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9NVM9.
MaxQBiQ9NVM9.
PaxDbiQ9NVM9.
PeptideAtlasiQ9NVM9.
PRIDEiQ9NVM9.

PTM databases

iPTMnetiQ9NVM9.
PhosphoSitePlusiQ9NVM9.

Expressioni

Tissue specificityi

Widely expressed. Tends to be up-regulated in seminomas compared to normal testis.1 Publication

Gene expression databases

BgeeiENSG00000064102.
CleanExiHS_C12orf11.
ExpressionAtlasiQ9NVM9. baseline and differential.
GenevisibleiQ9NVM9. HS.

Organism-specific databases

HPAiHPA039543.

Interactioni

Subunit structurei

Interacts with PAFAH1B1; this interaction may be required for proper recruitment of dynein complexes to the nuclear envelope at prophase.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CBFBQ139512EBI-741429,EBI-718750

Protein-protein interaction databases

BioGridi120846. 21 interactors.
IntActiQ9NVM9. 7 interactors.
MINTiMINT-6782559.
STRINGi9606.ENSP00000261191.

Structurei

3D structure databases

ProteinModelPortaliQ9NVM9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili567 – 622Sequence analysisAdd BLAST56

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi572 – 582Nuclear localization signal (NLS)1 PublicationAdd BLAST11

Sequence similaritiesi

Belongs to the asunder family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3711. Eukaryota.
ENOG410XS4B. LUCA.
GeneTreeiENSGT00390000002793.
HOGENOMiHOG000046389.
InParanoidiQ9NVM9.
OMAiLKSGDTH.
OrthoDBiEOG091G03Z1.
PhylomeDBiQ9NVM9.
TreeFamiTF105815.

Family and domain databases

InterProiView protein in InterPro
IPR019355. Cell_cycle_regulator_Mat89Bb.
PANTHERiPTHR12955:SF2. PTHR12955:SF2. 1 hit.
PfamiView protein in Pfam
PF10221. DUF2151. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NVM9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKIFSESHKT VFVVDHCPYM AESCRQHVEF DMLVKNRTQG IIPLAPISKS
60 70 80 90 100
LWTCSVESSM EYCRIMYDIF PFKKLVNFIV SDSGAHVLNS WTQEDQNLQE
110 120 130 140 150
LMAALAAVGP PNPRADPECC SILHGLVAAV ETLCKITEYQ HEARTLLMEN
160 170 180 190 200
AERVGNRGRI ICITNAKSDS HVRMLEDCVQ ETIHEHNKLA ANSDHLMQIQ
210 220 230 240 250
KCELVLIHTY PVGEDSLVSD RSKKELSPVL TSEVHSVRAG RHLATKLNIL
260 270 280 290 300
VQQHFDLAST TITNIPMKEE QHANTSANYD VELLHHKDAH VDFLKSGDSH
310 320 330 340 350
LGGGSREGSF KETITLKWCT PRTNNIELHY CTGAYRISPV DVNSRPSSCL
360 370 380 390 400
TNFLLNGRSV LLEQPRKSGS KVISHMLSSH GGEIFLHVLS SSRSILEDPP
410 420 430 440 450
SISEGCGGRV TDYRITDFGE FMRENRLTPF LDPRYKIDGS LEVPLERAKD
460 470 480 490 500
QLEKHTRYWP MIISQTTIFN MQAVVPLASV IVKESLTEED VLNCQKTIYN
510 520 530 540 550
LVDMERKNDP LPISTVGTRG KGPKRDEQYR IMWNELETLV RAHINNSEKH
560 570 580 590 600
QRVLECLMAC RSKPPEEEER KKRGRKREDK EDKSEKAVKD YEQEKSWQDS
610 620 630 640 650
ERLKGILERG KEELAEAEII KDSPDSPEPP NKKPLVEMDE TPQVEKSKGP
660 670 680 690 700
VSLLSLWSNR INTANSRKHQ EFAGRLNSVN NRAELYQHLK EENGMETTEN

GKASRQ
Length:706
Mass (Da):80,225
Last modified:July 19, 2005 - v2
Checksum:i925C733184461EA6
GO
Isoform 2 (identifier: Q9NVM9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-101: Missing.

Note: No experimental confirmation available.
Show »
Length:605
Mass (Da):68,494
Checksum:i7D9017DE546CF1BD
GO

Sequence cautioni

The sequence AAO65180 differs from that shown. Reason: Frameshift at positions 414, 445, 483, 498 and 507.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti29E → G in BAA91725 (PubMed:14702039).Curated1
Sequence conflicti53T → P in BAA91725 (PubMed:14702039).Curated1
Sequence conflicti75L → P in BAA91725 (PubMed:14702039).Curated1
Sequence conflicti454K → R in BAA91721 (PubMed:14702039).Curated1
Sequence conflicti457R → P in BAA91721 (PubMed:14702039).Curated1
Sequence conflicti459W → R in BAA91725 (PubMed:14702039).Curated1
Sequence conflicti484E → K in AAO65180 (PubMed:12601173).Curated1
Sequence conflicti490D → N in AAO65180 (PubMed:12601173).Curated1
Sequence conflicti699E → K in AAH03081 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05086466M → T. Corresponds to variant dbSNP:rs2306852Ensembl.1
Natural variantiVAR_035673227S → P in a colorectal cancer sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0565141 – 101Missing in isoform 2. 1 PublicationAdd BLAST101

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001492 mRNA. Translation: BAA91721.1.
AK001499 mRNA. Translation: BAA91725.1.
AK297948 mRNA. Translation: BAG60263.1.
AC024093 Genomic DNA. No translation available.
BC003081 mRNA. Translation: AAH03081.1.
BC008368 mRNA. Translation: AAH08368.1.
AY211927 mRNA. Translation: AAO65180.1. Frameshift.
AL137401 mRNA. Translation: CAB70726.1.
CCDSiCCDS8708.1. [Q9NVM9-1]
PIRiT46457.
RefSeqiNP_060634.2. NM_018164.2. [Q9NVM9-1]
XP_016875121.1. XM_017019632.1. [Q9NVM9-1]
UniGeneiHs.505077.

Genome annotation databases

EnsembliENST00000261191; ENSP00000261191; ENSG00000064102. [Q9NVM9-1]
GeneIDi55726.
KEGGihsa:55726.
UCSCiuc001rhk.5. human. [Q9NVM9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiINT13_HUMAN
AccessioniPrimary (citable) accession number: Q9NVM9
Secondary accession number(s): B4DNK1
, Q86WE2, Q96HM2, Q9BTX2, Q9NTB6, Q9NVM5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: September 27, 2017
This is version 114 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families