Q9NVM9 (ASUN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein asunder homolog

Alternative name(s):
Cell cycle regulator Mat89Bb homolog
Germ cell tumor 1
Sarcoma antigen NY-SAR-95

Gene names

Name:	ASUN
Synonyms:	C12orf11, GCT1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	706 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Crucial regulator of the mitotic cell cycle and development. At prophase, required for dynein anchoring to the nuclear envelope important for proper centrosome-nucleus coupling. At G2/M phase, may be required for proper spindle formation and execution of cytokinesis. Ref.6 Ref.12
Subunit structure	Interacts with PAFAH1B1; this interaction may be required for proper recruitment of dynein complexes to the nuclear envelope at prophase. Ref.12
Subcellular location	Cytoplasm Ref.12.
Tissue specificity	Widely expressed. Tends to be up-regulated in seminomas compared to normal testis. Ref.5
Miscellaneous	RNAi in HeLa cells results in a multinucleated phenotype.
Sequence similarities	Belongs to the asunder family.
Sequence caution	The sequence AAO65180.1 differs from that shown. Reason: Frameshift at positions 414, 445, 483, 498 and 507.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW regulation of fertilization Inferred from sequence or structural similarity. Source: UniProtKB regulation of mitotic cell cycle Inferred from mutant phenotype Ref.6. Source: UniProtKB sperm motility Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CBFB	Q13951	2	EBI-741429,EBI-718750

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 706	706	Protein asunder homolog		PRO_0000089845
Regions
	Coiled coil	567 – 622	56	Potential
Amino acid modifications
	Modified residue	626	1	Phosphoserine Ref.7 Ref.8 Ref.9
Natural variations
	Natural variant	66	1	M → T. Corresponds to variant rs2306852 [ dbSNP | Ensembl ].		VAR_050864
	Natural variant	227	1	S → P in a colorectal cancer sample; somatic mutation. Ref.13		VAR_035673
Experimental info
	Sequence conflict	29	1	E → G in BAA91725. Ref.1
	Sequence conflict	53	1	T → P in BAA91725. Ref.1
	Sequence conflict	75	1	L → P in BAA91725. Ref.1
	Sequence conflict	454	1	K → R in BAA91721. Ref.1
	Sequence conflict	457	1	R → P in BAA91721. Ref.1
	Sequence conflict	459	1	W → R in BAA91725. Ref.1
	Sequence conflict	484	1	E → K in AAO65180. Ref.3
	Sequence conflict	490	1	D → N in AAO65180. Ref.3
	Sequence conflict	699	1	E → K in AAH03081. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9NVM9 [UniParc]. Last modified July 19, 2005. Version 2. Checksum: 925C733184461EA6	FASTA	706	80,225
	10 20 30 40 50 60 MKIFSESHKT VFVVDHCPYM AESCRQHVEF DMLVKNRTQG IIPLAPISKS LWTCSVESSM 70 80 90 100 110 120 EYCRIMYDIF PFKKLVNFIV SDSGAHVLNS WTQEDQNLQE LMAALAAVGP PNPRADPECC 130 140 150 160 170 180 SILHGLVAAV ETLCKITEYQ HEARTLLMEN AERVGNRGRI ICITNAKSDS HVRMLEDCVQ 190 200 210 220 230 240 ETIHEHNKLA ANSDHLMQIQ KCELVLIHTY PVGEDSLVSD RSKKELSPVL TSEVHSVRAG 250 260 270 280 290 300 RHLATKLNIL VQQHFDLAST TITNIPMKEE QHANTSANYD VELLHHKDAH VDFLKSGDSH 310 320 330 340 350 360 LGGGSREGSF KETITLKWCT PRTNNIELHY CTGAYRISPV DVNSRPSSCL TNFLLNGRSV 370 380 390 400 410 420 LLEQPRKSGS KVISHMLSSH GGEIFLHVLS SSRSILEDPP SISEGCGGRV TDYRITDFGE 430 440 450 460 470 480 FMRENRLTPF LDPRYKIDGS LEVPLERAKD QLEKHTRYWP MIISQTTIFN MQAVVPLASV 490 500 510 520 530 540 IVKESLTEED VLNCQKTIYN LVDMERKNDP LPISTVGTRG KGPKRDEQYR IMWNELETLV 550 560 570 580 590 600 RAHINNSEKH QRVLECLMAC RSKPPEEEER KKRGRKREDK EDKSEKAVKD YEQEKSWQDS 610 620 630 640 650 660 ERLKGILERG KEELAEAEII KDSPDSPEPP NKKPLVEMDE TPQVEKSKGP VSLLSLWSNR 670 680 690 700 INTANSRKHQ EFAGRLNSVN NRAELYQHLK EENGMETTEN GKASRQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye and Lymph.
[3]	"Immunomic analysis of human sarcoma." Lee S.-Y., Obata Y., Yoshida M., Stockert E., Williamson B., Jungbluth A.A., Chen Y.-T., Old L.J., Scanlan M.J. Proc. Natl. Acad. Sci. U.S.A. 100:2651-2656(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 269-513.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 568-706. Tissue: Testis.
[5]	"Genomic and expression analysis of the 12p11-p12 amplicon using EST arrays identifies two novel amplified and overexpressed genes." Bourdon V., Naef F., Rao P.H., Reuter V., Mok S.C., Bosl G.J., Koul S., Murty V.V., Kucherlapati R.S., Chaganti R.S. Cancer Res. 62:6218-6223(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"Drosophila genome-scale screen for PAN GU kinase substrates identifies Mat89Bb as a cell cycle regulator." Lee L.A., Lee E., Anderson M.A., Vardy L., Tahinci E., Ali S.M., Kashevsky H., Benasutti M., Kirschner M.W., Orr-Weaver T.L. Dev. Cell 8:435-442(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-626, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-626, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-626, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]	"Human Asunder promotes dynein recruitment and centrosomal tethering to the nucleus at mitotic entry." Jodoin J.N., Shboul M., Sitaram P., Zein-Sabatto H., Reversade B., Lee E., Lee L.A. Mol. Biol. Cell 23:4713-4724(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH PAFAH1B1, SUBCELLULAR LOCATION.
[13]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] PRO-227.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK001492 mRNA. Translation: BAA91721.1. AK001499 mRNA. Translation: BAA91725.1. BC003081 mRNA. Translation: AAH03081.1. BC008368 mRNA. Translation: AAH08368.1. AY211927 mRNA. Translation: AAO65180.1. Frameshift. AL137401 mRNA. Translation: CAB70726.1.
IPI	IPI00550986.
PIR	T46457.
RefSeq	NP_060634.2. NM_018164.2.
UniGene	Hs.505077.
3D structure databases
ProteinModelPortal	Q9NVM9.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NVM9. 3 interactions.
STRING	9606.ENSP00000261191.
PTM databases
PhosphoSite	Q9NVM9.
Polymorphism databases
DMDM	71153010.
Proteomic databases
PaxDb	Q9NVM9.
PeptideAtlas	Q9NVM9.
PRIDE	Q9NVM9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000261191; ENSP00000261191; ENSG00000064102.
GeneID	55726.
KEGG	hsa:55726.
UCSC	uc001rhk.4. human.
Organism-specific databases
CTD	55726.
GeneCards	GC12M027059.
H-InvDB	HIX0010501.
HGNC	HGNC:20174. ASUN.
HPA	HPA039543.
MIM	615079. gene.
neXtProt	NX_Q9NVM9.
PharmGKB	PA134892469.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG301038.
HOGENOM	HOG000046389.
InParanoid	Q9NVM9.
OMA	GRIICLT.
OrthoDB	EOG43N7C5.
PhylomeDB	Q9NVM9.
Gene expression databases
ArrayExpress	Q9NVM9.
Bgee	Q9NVM9.
CleanEx	HS_C12orf11.
Genevestigator	Q9NVM9.
GermOnline	ENSG00000064102. Homo sapiens.
Family and domain databases
InterPro	IPR019355. Cell_cycle_regulator_Mat89Bb. [Graphical view]
Pfam	PF10221. DUF2151. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	55726.
NextBio	60635.
SOURCE	Search...

Entry information

Entry name

ASUN_HUMAN

Accession

Primary (citable) accession number: Q9NVM9
Secondary accession number(s): Q86WE2 , Q96HM2, Q9BTX2, Q9NTB6, Q9NVM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	May 1, 2013
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.