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Protein

ADP-ribosylation factor-like protein 8B

Gene

ARL8B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in lysosome motility (PubMed:16537643, PubMed:25898167). May play a role in chromosome segregation (PubMed:15331635).3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi29 – 35GTP7
Nucleotide bindingi71 – 75GTPBy similarity5
Nucleotide bindingi130 – 133GTP4

GO - Molecular functioni

  • alpha-tubulin binding Source: UniProtKB
  • beta-tubulin binding Source: UniProtKB
  • GDP binding Source: UniProtKB
  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: UniProtKB
  • lysosome localization Source: UniProtKB
  • small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000134108-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-like protein 8B
Alternative name(s):
ADP-ribosylation factor-like protein 10C
Novel small G protein indispensable for equal chromosome segregation 1
Gene namesi
Name:ARL8B
Synonyms:ARL10C, GIE1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:25564. ARL8B.

Subcellular locationi

  • Late endosome membrane 1 Publication
  • Lysosome membrane 1 Publication
  • Cytoplasmcytoskeletonspindle

  • Note: Localizes with microtubules at the spindle mid-zone during mitosis.1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Intramembranei1 – 19Note=Mediates targeting to membranesAdd BLAST19

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • late endosome membrane Source: UniProtKB-SubCell
  • lysosomal membrane Source: UniProtKB-SubCell
  • membrane Source: UniProtKB
  • midbody Source: UniProtKB
  • spindle midzone Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Endosome, Lysosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2L → A: Diffuse cytoplasmic distribution and loss of localization to lysosomes. No effect on acetylation. 1 Publication1
Mutagenesisi2L → F: No effect on localization and acetylation. 1 Publication1
Mutagenesisi5 – 12ISRLLDWF → ASRALDWA: Diffuse cytoplasmic distribution and loss of localization to lysosomes. No effect on acetylation. 1 Publication8
Mutagenesisi34T → N: Preferentially binds GDP. Alters chromosome segregation. 1 Publication1
Mutagenesisi49 – 58Missing : Alters chromosome segregation. 1 Publication10
Mutagenesisi70W → R: Preferentially binds GTP. 1 Publication1
Mutagenesisi74 – 85Missing : Alters chromosome segregation. 1 PublicationAdd BLAST12
Mutagenesisi75Q → L: Prevents GTP hydrolysis. No effect on localization. Alters lysosomes cellular distribution and motility. 1 Publication1
Mutagenesisi130N → I: Loss of GTP/GDP-binding. Affects chromosome segregation. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000134108.
PharmGKBiPA134959531.

Polymorphism and mutation databases

BioMutaiARL8B.
DMDMi74752996.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002329211 – 186ADP-ribosylation factor-like protein 8BAdd BLAST186

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9NVJ2.
MaxQBiQ9NVJ2.
PaxDbiQ9NVJ2.
PeptideAtlasiQ9NVJ2.
PRIDEiQ9NVJ2.
TopDownProteomicsiQ9NVJ2-1. [Q9NVJ2-1]

PTM databases

iPTMnetiQ9NVJ2.
PhosphoSitePlusiQ9NVJ2.
SwissPalmiQ9NVJ2.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000134108.
CleanExiHS_ARL8B.
ExpressionAtlasiQ9NVJ2. baseline and differential.
GenevisibleiQ9NVJ2. HS.

Organism-specific databases

HPAiHPA038759.
HPA040515.
HPA045924.

Interactioni

Subunit structurei

Interacts with tubulin (PubMed:15331635, Ref. 16). Interacts with BORCS5; recruits ARL8B to lysosomes (PubMed:25898167).3 Publications

GO - Molecular functioni

  • alpha-tubulin binding Source: UniProtKB
  • beta-tubulin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi120503. 31 interactors.
IntActiQ9NVJ2. 10 interactors.
MINTiMINT-1429448.
STRINGi9606.ENSP00000256496.

Structurei

Secondary structure

1186
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi19 – 26Combined sources8
Helixi33 – 40Combined sources8
Beta strandi55 – 62Combined sources8
Beta strandi65 – 72Combined sources8
Helixi76 – 81Combined sources6
Helixi82 – 86Combined sources5
Beta strandi90 – 97Combined sources8
Helixi101 – 103Combined sources3
Helixi104 – 115Combined sources12
Helixi118 – 120Combined sources3
Beta strandi125 – 130Combined sources6
Helixi140 – 146Combined sources7
Helixi149 – 151Combined sources3
Beta strandi157 – 161Combined sources5
Turni164 – 167Combined sources4
Helixi170 – 178Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AL7X-ray1.85A18-184[»]
ProteinModelPortaliQ9NVJ2.
SMRiQ9NVJ2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NVJ2.

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0075. Eukaryota.
ENOG410XNUK. LUCA.
GeneTreeiENSGT00830000128269.
HOGENOMiHOG000163692.
HOVERGENiHBG105214.
InParanoidiQ9NVJ2.
KOiK07955.
OMAiKNDLEGH.
OrthoDBiEOG091G0USZ.
PhylomeDBiQ9NVJ2.
TreeFamiTF105470.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NVJ2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLALISRLLD WFRSLFWKEE MELTLVGLQY SGKTTFVNVI ASGQFSEDMI
60 70 80 90 100
PTVGFNMRKV TKGNVTIKIW DIGGQPRFRS MWERYCRGVN AIVYMIDAAD
110 120 130 140 150
REKIEASRNE LHNLLDKPQL QGIPVLVLGN KRDLPNALDE KQLIEKMNLS
160 170 180
AIQDREICCY SISCKEKDNI DITLQWLIQH SKSRRS
Length:186
Mass (Da):21,539
Last modified:October 1, 2000 - v1
Checksum:i5030B01AFD749223
GO
Isoform 2 (identifier: Q9NVJ2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     125-186: VLVLGNKRDL...LIQHSKSRRS → ISHFSGLFSI...ILELLSVPLK

Note: No experimental confirmation available.
Show »
Length:163
Mass (Da):18,642
Checksum:iBAD320672EA343D5
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056238125 – 186VLVLG…KSRRS → ISHFSGLFSIQNLEEAEASP EVFQSFLAIILELLSVPLK in isoform 2. 1 PublicationAdd BLAST62

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB118751 mRNA. Translation: BAD23992.1.
AK001564 mRNA. Translation: BAA91759.1.
AK295465 mRNA. Translation: BAG58397.1.
CR457264 mRNA. Translation: CAG33545.1.
AC021999 Genomic DNA. No translation available.
AC026202 Genomic DNA. No translation available.
CH471055 Genomic DNA. Translation: EAW63919.1.
BC013131 mRNA. Translation: AAH13131.1.
BC063125 mRNA. Translation: AAH63125.1.
CCDSiCCDS2566.1. [Q9NVJ2-1]
RefSeqiNP_060654.1. NM_018184.2. [Q9NVJ2-1]
UniGeneiHs.250009.

Genome annotation databases

EnsembliENST00000256496; ENSP00000256496; ENSG00000134108. [Q9NVJ2-1]
ENST00000419534; ENSP00000402996; ENSG00000134108. [Q9NVJ2-2]
ENST00000611208; ENSP00000479202; ENSG00000134108. [Q9NVJ2-1]
GeneIDi55207.
KEGGihsa:55207.
UCSCiuc003bqg.4. human. [Q9NVJ2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB118751 mRNA. Translation: BAD23992.1.
AK001564 mRNA. Translation: BAA91759.1.
AK295465 mRNA. Translation: BAG58397.1.
CR457264 mRNA. Translation: CAG33545.1.
AC021999 Genomic DNA. No translation available.
AC026202 Genomic DNA. No translation available.
CH471055 Genomic DNA. Translation: EAW63919.1.
BC013131 mRNA. Translation: AAH13131.1.
BC063125 mRNA. Translation: AAH63125.1.
CCDSiCCDS2566.1. [Q9NVJ2-1]
RefSeqiNP_060654.1. NM_018184.2. [Q9NVJ2-1]
UniGeneiHs.250009.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AL7X-ray1.85A18-184[»]
ProteinModelPortaliQ9NVJ2.
SMRiQ9NVJ2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120503. 31 interactors.
IntActiQ9NVJ2. 10 interactors.
MINTiMINT-1429448.
STRINGi9606.ENSP00000256496.

PTM databases

iPTMnetiQ9NVJ2.
PhosphoSitePlusiQ9NVJ2.
SwissPalmiQ9NVJ2.

Polymorphism and mutation databases

BioMutaiARL8B.
DMDMi74752996.

Proteomic databases

EPDiQ9NVJ2.
MaxQBiQ9NVJ2.
PaxDbiQ9NVJ2.
PeptideAtlasiQ9NVJ2.
PRIDEiQ9NVJ2.
TopDownProteomicsiQ9NVJ2-1. [Q9NVJ2-1]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000256496; ENSP00000256496; ENSG00000134108. [Q9NVJ2-1]
ENST00000419534; ENSP00000402996; ENSG00000134108. [Q9NVJ2-2]
ENST00000611208; ENSP00000479202; ENSG00000134108. [Q9NVJ2-1]
GeneIDi55207.
KEGGihsa:55207.
UCSCiuc003bqg.4. human. [Q9NVJ2-1]

Organism-specific databases

CTDi55207.
GeneCardsiARL8B.
HGNCiHGNC:25564. ARL8B.
HPAiHPA038759.
HPA040515.
HPA045924.
MIMi616596. gene.
neXtProtiNX_Q9NVJ2.
OpenTargetsiENSG00000134108.
PharmGKBiPA134959531.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0075. Eukaryota.
ENOG410XNUK. LUCA.
GeneTreeiENSGT00830000128269.
HOGENOMiHOG000163692.
HOVERGENiHBG105214.
InParanoidiQ9NVJ2.
KOiK07955.
OMAiKNDLEGH.
OrthoDBiEOG091G0USZ.
PhylomeDBiQ9NVJ2.
TreeFamiTF105470.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000134108-MONOMER.

Miscellaneous databases

ChiTaRSiARL8B. human.
EvolutionaryTraceiQ9NVJ2.
GeneWikiiARL8B.
GenomeRNAii55207.
PROiQ9NVJ2.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000134108.
CleanExiHS_ARL8B.
ExpressionAtlasiQ9NVJ2. baseline and differential.
GenevisibleiQ9NVJ2. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARL8B_HUMAN
AccessioniPrimary (citable) accession number: Q9NVJ2
Secondary accession number(s): B4DI85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.