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Q9NVJ2 (ARL8B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ADP-ribosylation factor-like protein 8B
Alternative name(s):
ADP-ribosylation factor-like protein 10C
Novel small G protein indispensable for equal chromosome segregation 1
Gene names
Name:ARL8B
Synonyms:ARL10C, GIE1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length186 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a role in lysosome motility (Ref.6). May play a role in chromosome segregation (Ref.1). Ref.1 Ref.6

Subunit structure

Interacts with tubulin. Ref.1

Subcellular location

Late endosome membrane. Lysosome membrane. Cytoplasmcytoskeletonspindle. Note: According to Ref.1, it localizes with microtubules at the spindle mid-zone during mitosis. Ref.1 Ref.6 Ref.7

Tissue specificity

Ubiquitously expressed. Ref.1

Sequence similarities

Belongs to the small GTPase superfamily. Arf family.

Ontologies

Keywords
   Biological processCell cycle
Cell division
   Cellular componentCytoplasm
Cytoskeleton
Endosome
Lysosome
Membrane
   LigandGTP-binding
Nucleotide-binding
   PTMAcetylation
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processGTP catabolic process

Non-traceable author statement Ref.1. Source: GOC

cell cycle

Inferred from electronic annotation. Source: UniProtKB-KW

cell division

Inferred from electronic annotation. Source: UniProtKB-KW

chromosome segregation

Inferred from mutant phenotype PubMed 14871887. Source: UniProtKB

small GTPase mediated signal transduction

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from direct assay Ref.1. Source: UniProtKB

extracellular vesicular exosome

Inferred from direct assay PubMed 19056867. Source: UniProt

late endosome membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

lysosomal membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

midbody

Inferred from direct assay Ref.1. Source: UniProtKB

spindle midzone

Inferred from direct assay Ref.1. Source: UniProtKB

   Molecular_functionGDP binding

Inferred from direct assay Ref.1. Source: UniProtKB

GTP binding

Inferred from direct assay Ref.1. Source: UniProtKB

GTPase activity

Non-traceable author statement Ref.1. Source: UniProtKB

alpha-tubulin binding

Inferred from direct assay Ref.1. Source: UniProtKB

beta-tubulin binding

Inferred from direct assay Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 186186ADP-ribosylation factor-like protein 8B
PRO_0000232921

Regions

Intramembrane1 – 1919Note=Mediates targeting to membranes
Nucleotide binding29 – 357GTP
Nucleotide binding71 – 755GTP By similarity
Nucleotide binding130 – 1334GTP

Amino acid modifications

Modified residue11N-acetylmethionine Ref.6 Ref.9 Ref.10

Experimental info

Mutagenesis21L → A: Diffuse cytoplasmic distribution and loss of localization to lysosomes. No effect on acetylation. Ref.6
Mutagenesis21L → F: No effect on localization and acetylation. Ref.6
Mutagenesis5 – 128ISRLLDWF → ASRALDWA: Diffuse cytoplasmic distribution and loss of localization to lysosomes. No effect on acetylation. Ref.6
Mutagenesis341T → N: Preferentially binds GDP. Alters chromosome segregation. Ref.1
Mutagenesis49 – 5810Missing: Alters chromosome segregation. Ref.1
Mutagenesis701W → R: Preferentially binds GTP. Ref.1
Mutagenesis74 – 8512Missing: Alters chromosome segregation. Ref.1
Mutagenesis751Q → L: Prevents GTP hydrolysis. No effect on localization. Alters lysosomes cellular distribution and motility. Ref.6
Mutagenesis1301N → I: Loss of GTP/GDP-binding. Affects chromosome segregation. Ref.1

Secondary structure

............................... 186
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9NVJ2 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 5030B01AFD749223

FASTA18621,539
        10         20         30         40         50         60 
MLALISRLLD WFRSLFWKEE MELTLVGLQY SGKTTFVNVI ASGQFSEDMI PTVGFNMRKV 

        70         80         90        100        110        120 
TKGNVTIKIW DIGGQPRFRS MWERYCRGVN AIVYMIDAAD REKIEASRNE LHNLLDKPQL 

       130        140        150        160        170        180 
QGIPVLVLGN KRDLPNALDE KQLIEKMNLS AIQDREICCY SISCKEKDNI DITLQWLIQH 


SKSRRS 

« Hide

References

« Hide 'large scale' references
[1]"Novel small GTPase subfamily capable of associating with tubulin is required for chromosome segregation."
Okai T., Araki Y., Tada M., Tateno T., Kontani K., Katada T.
J. Cell Sci. 117:4705-4715(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF THR-34; 49-MET--ARG-58; TRP-70; 74-GLY--TYR-85 AND ASN-130, SUBCELLULAR LOCATION, INTERACTION WITH TUBULIN.
Tissue: Brain.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Teratocarcinoma.
[3]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Embryonic carcinoma and Testis.
[5]Bienvenut W.V.
Submitted (JUN-2005) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 69-77; 147-155 AND 166-182, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: B-cell lymphoma.
[6]"An N-terminally acetylated Arf-like GTPase is localised to lysosomes and affects their motility."
Hofmann I., Munro S.
J. Cell Sci. 119:1494-1503(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, ACETYLATION AT MET-1, MUTAGENESIS OF LEU-2; 5-ILE--PHE-12 AND GLN-75.
[7]"Integral and associated lysosomal membrane proteins."
Schroeder B., Wrocklage C., Pan C., Jaeger R., Koesters B., Schaefer H., Elsaesser H.-P., Mann M., Hasilik A.
Traffic 8:1676-1686(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Tissue: Placenta.
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"GTP-like conformation of GDP-bound ARL10C GTPase."
Structural genomics consortium (SGC)
Submitted (AUG-2005) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 18-184 IN COMPLEX WITH GDP.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB118751 mRNA. Translation: BAD23992.1.
AK001564 mRNA. Translation: BAA91759.1.
CR457264 mRNA. Translation: CAG33545.1.
BC013131 mRNA. Translation: AAH13131.1.
BC063125 mRNA. Translation: AAH63125.1.
RefSeqNP_060654.1. NM_018184.2.
UniGeneHs.250009.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2AL7X-ray1.85A18-184[»]
ProteinModelPortalQ9NVJ2.
SMRQ9NVJ2. Positions 18-182.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120503. 8 interactions.
IntActQ9NVJ2. 7 interactions.
MINTMINT-1429448.
STRING9606.ENSP00000256496.

PTM databases

PhosphoSiteQ9NVJ2.

Polymorphism databases

DMDM74752996.

Proteomic databases

PaxDbQ9NVJ2.
PRIDEQ9NVJ2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000256496; ENSP00000256496; ENSG00000134108.
GeneID55207.
KEGGhsa:55207.
UCSCuc003bqg.3. human.

Organism-specific databases

CTD55207.
GeneCardsGC03P005163.
HGNCHGNC:25564. ARL8B.
HPAHPA038759.
HPA040515.
HPA045924.
neXtProtNX_Q9NVJ2.
PharmGKBPA134959531.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1100.
HOGENOMHOG000163692.
HOVERGENHBG105214.
InParanoidQ9NVJ2.
KOK07955.
OMASIQDREI.
OrthoDBEOG7F24V2.
PhylomeDBQ9NVJ2.
TreeFamTF105470.

Gene expression databases

ArrayExpressQ9NVJ2.
BgeeQ9NVJ2.
CleanExHS_ARL8B.
GenevestigatorQ9NVJ2.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamPF00025. Arf. 1 hit.
[Graphical view]
PRINTSPR00328. SAR1GTPBP.
SMARTSM00177. ARF. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSARL8B. human.
EvolutionaryTraceQ9NVJ2.
GeneWikiARL8B.
GenomeRNAi55207.
NextBio59110.
PROQ9NVJ2.

Entry information

Entry nameARL8B_HUMAN
AccessionPrimary (citable) accession number: Q9NVJ2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM