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Protein

Ethanolamine kinase 2

Gene

ETNK2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Highly specific for ethanolamine phosphorylation. Does not have choline kinase activity (By similarity).By similarity

Catalytic activityi

ATP + ethanolamine = ADP + O-phosphoethanolamine.

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ethanolamine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycerophospholipid biosynthetic process Source: Reactome
  2. in utero embryonic development Source: Ensembl
  3. multicellular organism growth Source: Ensembl
  4. phosphatidylethanolamine biosynthetic process Source: Reactome
  5. phospholipid metabolic process Source: Reactome
  6. placenta development Source: Ensembl
  7. post-embryonic development Source: Ensembl
  8. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_120919. Synthesis of PE.
UniPathwayiUPA00558; UER00741.

Names & Taxonomyi

Protein namesi
Recommended name:
Ethanolamine kinase 2 (EC:2.7.1.82)
Short name:
EKI 2
Alternative name(s):
Ethanolamine kinase-like protein
Gene namesi
Name:ETNK2
Synonyms:EKI2
ORF Names:HMFT1716
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 1

Organism-specific databases

HGNCiHGNC:25575. ETNK2.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Reactome
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134888760.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 386386Ethanolamine kinase 2PRO_0000206229Add
BLAST

Proteomic databases

PaxDbiQ9NVF9.
PRIDEiQ9NVF9.

Expressioni

Tissue specificityi

Expressed in kidney, liver, ovary, testis and prostate.1 Publication

Gene expression databases

BgeeiQ9NVF9.
CleanExiHS_ETNK2.
ExpressionAtlasiQ9NVF9. baseline and differential.
GenevestigatoriQ9NVF9.

Organism-specific databases

HPAiHPA057167.

Interactioni

Protein-protein interaction databases

BioGridi120519. 4 interactions.
IntActiQ9NVF9. 3 interactions.
STRINGi9606.ENSP00000356170.

Structurei

3D structure databases

ProteinModelPortaliQ9NVF9.
SMRiQ9NVF9. Positions 62-381.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the choline/ethanolamine kinase family.Curated

Phylogenomic databases

eggNOGiCOG0510.
GeneTreeiENSGT00530000062991.
HOGENOMiHOG000004856.
HOVERGENiHBG018981.
InParanoidiQ9NVF9.
KOiK00894.
OMAiSLGPHPE.
OrthoDBiEOG72NRQH.
PhylomeDBiQ9NVF9.
TreeFamiTF313549.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9NVF9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVPPSAPQP RASFHLRRHT PCPQCSWGME EKAAASASCR EPPGPPRAAA
60 70 80 90 100
VAYFGISVDP DDILPGALRL IQELRPHWKP EQVRTKRFTD GITNKLVACY
110 120 130 140 150
VEEDMQDCVL VRVYGERTEL LVDRENEVRN FQLLRAHSCA PKLYCTFQNG
160 170 180 190 200
LCYEYMQGVA LEPEHIREPR LFRLIALEMA KIHTIHANGS LPKPILWHKM
210 220 230 240 250
HNYFTLVKNE INPSLSADVP KVEVLERELA WLKEHLSQLE SPVVFCHNDL
260 270 280 290 300
LCKNIIYDSI KGHVRFIDYE YAGYNYQAFD IGNHFNEFAG VNEVDYCLYP
310 320 330 340 350
ARETQLQWLH YYLQAQKGMA VTPREVQRLY VQVNKFALAS HFFWALWALI
360 370 380
QNQYSTIDFD FLRYAVIRFN QYFKVKPQAS ALEMPK
Length:386
Mass (Da):44,781
Last modified:May 18, 2010 - v3
Checksum:i341E981AD2B80C15
GO
Isoform 2 (identifier: Q9NVF9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     339-386: ASHFFWALWA...PQASALEMPK → GPSCVSSTMT...HPEPVLHHRL

Show »
Length:394
Mass (Da):44,841
Checksum:i1A334DF65649D885
GO
Isoform 3 (identifier: Q9NVF9-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     173-213: Missing.

Note: No experimental confirmation available.

Show »
Length:345
Mass (Da):40,009
Checksum:iDF9063552B7E5E2E
GO

Sequence cautioni

The sequence BAH13637.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 101P → Q in BAA91793. (PubMed:14702039)Curated
Sequence conflicti148 – 1481Q → R in BAH13637. (PubMed:14702039)Curated
Sequence conflicti241 – 2411S → F in BAH13637. (PubMed:14702039)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti227 – 2271R → Q.1 Publication
Corresponds to variant rs3737657 [ dbSNP | Ensembl ].
VAR_022145

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei173 – 21341Missing in isoform 3. 1 PublicationVSP_039558Add
BLAST
Alternative sequencei339 – 38648ASHFF…LEMPK → GPSCVSSTMTASLQCCRVGN RHGEIARLTLSGLFPGVSLL LGSLGPHPEPVLHHRL in isoform 2. 1 PublicationVSP_039098Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001623 mRNA. Translation: BAA91793.1.
AK302145 mRNA. Translation: BAH13637.1. Different initiation.
AL592146 Genomic DNA. Translation: CAI16602.1.
AL592146 Genomic DNA. Translation: CAI16603.1.
CH471067 Genomic DNA. Translation: EAW91501.1.
CH471067 Genomic DNA. Translation: EAW91502.1.
BC010082 mRNA. Translation: AAH10082.1.
AB073608 mRNA. Translation: BAD38645.1.
CCDSiCCDS1442.2. [Q9NVF9-1]
CCDS73006.1. [Q9NVF9-2]
RefSeqiNP_001284689.1. NM_001297760.1. [Q9NVF9-2]
NP_001284690.1. NM_001297761.1.
NP_001284691.1. NM_001297762.1. [Q9NVF9-3]
NP_060678.2. NM_018208.3. [Q9NVF9-1]
UniGeneiHs.497469.

Genome annotation databases

EnsembliENST00000367201; ENSP00000356169; ENSG00000143845. [Q9NVF9-2]
ENST00000367202; ENSP00000356170; ENSG00000143845. [Q9NVF9-1]
GeneIDi55224.
KEGGihsa:55224.
UCSCiuc001han.4. human. [Q9NVF9-2]
uc001hao.4. human. [Q9NVF9-1]
uc010pqs.2. human. [Q9NVF9-3]

Polymorphism databases

DMDMi296439366.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001623 mRNA. Translation: BAA91793.1.
AK302145 mRNA. Translation: BAH13637.1. Different initiation.
AL592146 Genomic DNA. Translation: CAI16602.1.
AL592146 Genomic DNA. Translation: CAI16603.1.
CH471067 Genomic DNA. Translation: EAW91501.1.
CH471067 Genomic DNA. Translation: EAW91502.1.
BC010082 mRNA. Translation: AAH10082.1.
AB073608 mRNA. Translation: BAD38645.1.
CCDSiCCDS1442.2. [Q9NVF9-1]
CCDS73006.1. [Q9NVF9-2]
RefSeqiNP_001284689.1. NM_001297760.1. [Q9NVF9-2]
NP_001284690.1. NM_001297761.1.
NP_001284691.1. NM_001297762.1. [Q9NVF9-3]
NP_060678.2. NM_018208.3. [Q9NVF9-1]
UniGeneiHs.497469.

3D structure databases

ProteinModelPortaliQ9NVF9.
SMRiQ9NVF9. Positions 62-381.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120519. 4 interactions.
IntActiQ9NVF9. 3 interactions.
STRINGi9606.ENSP00000356170.

Polymorphism databases

DMDMi296439366.

Proteomic databases

PaxDbiQ9NVF9.
PRIDEiQ9NVF9.

Protocols and materials databases

DNASUi55224.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000367201; ENSP00000356169; ENSG00000143845. [Q9NVF9-2]
ENST00000367202; ENSP00000356170; ENSG00000143845. [Q9NVF9-1]
GeneIDi55224.
KEGGihsa:55224.
UCSCiuc001han.4. human. [Q9NVF9-2]
uc001hao.4. human. [Q9NVF9-1]
uc010pqs.2. human. [Q9NVF9-3]

Organism-specific databases

CTDi55224.
GeneCardsiGC01M204100.
H-InvDBHIX0001494.
HGNCiHGNC:25575. ETNK2.
HPAiHPA057167.
MIMi609859. gene.
neXtProtiNX_Q9NVF9.
PharmGKBiPA134888760.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0510.
GeneTreeiENSGT00530000062991.
HOGENOMiHOG000004856.
HOVERGENiHBG018981.
InParanoidiQ9NVF9.
KOiK00894.
OMAiSLGPHPE.
OrthoDBiEOG72NRQH.
PhylomeDBiQ9NVF9.
TreeFamiTF313549.

Enzyme and pathway databases

UniPathwayiUPA00558; UER00741.
ReactomeiREACT_120919. Synthesis of PE.

Miscellaneous databases

ChiTaRSiETNK2. human.
GenomeRNAii55224.
NextBioi59212.
PROiQ9NVF9.
SOURCEiSearch...

Gene expression databases

BgeeiQ9NVF9.
CleanExiHS_ETNK2.
ExpressionAtlasiQ9NVF9. baseline and differential.
GenevestigatoriQ9NVF9.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 25-386 (ISOFORM 3).
    Tissue: Testis.
  2. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Uterus.
  5. "Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas."
    Yamada S., Ohira M., Horie H., Ando K., Takayasu H., Suzuki Y., Sugano S., Hirata T., Goto T., Matsunaga T., Hiyama E., Hayashi Y., Ando H., Suita S., Kaneko M., Sasaki F., Hashizume K., Ohnuma N., Nakagawara A.
    Oncogene 23:5901-5911(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 25-386 (ISOFORM 1), VARIANT GLN-227.
    Tissue: Hepatoblastoma.
  6. "Overexpression of a mammalian ethanolamine-specific kinase accelerates the CDP-ethanolamine pathway."
    Lykidis A., Wang J., Karim M.A., Jackowski S.
    J. Biol. Chem. 276:2174-2179(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiEKI2_HUMAN
AccessioniPrimary (citable) accession number: Q9NVF9
Secondary accession number(s): B7Z7K1
, Q5SXX5, Q68CK3, Q96G05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: May 18, 2010
Last modified: January 7, 2015
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.