Q9NVF9 (EKI2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ethanolamine kinase 2
Short name=EKI 2
EC=2.7.1.82

Alternative name(s):
Ethanolamine kinase-like protein

Gene names

Name:	ETNK2
Synonyms:	EKI2
ORF Names:	HMFT1716

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Highly specific for ethanolamine phosphorylation. Does not have choline kinase activity By similarity.
Catalytic activity	ATP + ethanolamine = ADP + O-phosphoethanolamine.
Pathway	Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3.
Tissue specificity	Expressed in kidney, liver, ovary, testis and prostate. Ref.6
Sequence similarities	Belongs to the choline/ethanolamine kinase family.
Sequence caution	The sequence BAH13637.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Phospholipid biosynthesis Phospholipid metabolism
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	in utero embryonic development Inferred from electronic annotation. Source: Compara multicellular organism growth Inferred from electronic annotation. Source: Compara phosphatidylethanolamine biosynthetic process Traceable author statement. Source: Reactome placenta development Inferred from electronic annotation. Source: Compara post-embryonic development Inferred from electronic annotation. Source: Compara small molecule metabolic process Traceable author statement. Source: Reactome
Cellular_component	cytosol Traceable author statement. Source: Reactome
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ethanolamine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NVF9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NVF9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     339-386: ASHFFWALWA...PQASALEMPK → GPSCVSSTMT...HPEPVLHHRL

Isoform 3 (identifier: Q9NVF9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     173-213: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 386	386	Ethanolamine kinase 2		PRO_0000206229
Natural variations
	Alternative sequence	173 – 213	41	Missing in isoform 3.		VSP_039558
	Alternative sequence	339 – 386	48	ASHFF…LEMPK → GPSCVSSTMTASLQCCRVGN RHGEIARLTLSGLFPGVSLL LGSLGPHPEPVLHHRL in isoform 2.		VSP_039098
	Natural variant	227	1	R → Q. Ref.5 Corresponds to variant rs3737657 [ dbSNP | Ensembl ].		VAR_022145
Experimental info
	Sequence conflict	10	1	P → Q in BAA91793. Ref.1
	Sequence conflict	148	1	Q → R in BAH13637. Ref.1
	Sequence conflict	241	1	S → F in BAH13637. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 18, 2010. Version 3. Checksum: 341E981AD2B80C15	FASTA	386	44,781
	10 20 30 40 50 60 MAVPPSAPQP RASFHLRRHT PCPQCSWGME EKAAASASCR EPPGPPRAAA VAYFGISVDP 70 80 90 100 110 120 DDILPGALRL IQELRPHWKP EQVRTKRFTD GITNKLVACY VEEDMQDCVL VRVYGERTEL 130 140 150 160 170 180 LVDRENEVRN FQLLRAHSCA PKLYCTFQNG LCYEYMQGVA LEPEHIREPR LFRLIALEMA 190 200 210 220 230 240 KIHTIHANGS LPKPILWHKM HNYFTLVKNE INPSLSADVP KVEVLERELA WLKEHLSQLE 250 260 270 280 290 300 SPVVFCHNDL LCKNIIYDSI KGHVRFIDYE YAGYNYQAFD IGNHFNEFAG VNEVDYCLYP 310 320 330 340 350 360 ARETQLQWLH YYLQAQKGMA VTPREVQRLY VQVNKFALAS HFFWALWALI QNQYSTIDFD 370 380 FLRYAVIRFN QYFKVKPQAS ALEMPK
	Isoform 2 [UniParc]. Checksum: 1A334DF65649D885	FASTA	394	44,841
	10 20 30 40 50 60 MAVPPSAPQP RASFHLRRHT PCPQCSWGME EKAAASASCR EPPGPPRAAA VAYFGISVDP 70 80 90 100 110 120 DDILPGALRL IQELRPHWKP EQVRTKRFTD GITNKLVACY VEEDMQDCVL VRVYGERTEL 130 140 150 160 170 180 LVDRENEVRN FQLLRAHSCA PKLYCTFQNG LCYEYMQGVA LEPEHIREPR LFRLIALEMA 190 200 210 220 230 240 KIHTIHANGS LPKPILWHKM HNYFTLVKNE INPSLSADVP KVEVLERELA WLKEHLSQLE 250 260 270 280 290 300 SPVVFCHNDL LCKNIIYDSI KGHVRFIDYE YAGYNYQAFD IGNHFNEFAG VNEVDYCLYP 310 320 330 340 350 360 ARETQLQWLH YYLQAQKGMA VTPREVQRLY VQVNKFALGP SCVSSTMTAS LQCCRVGNRH 370 380 390 GEIARLTLSG LFPGVSLLLG SLGPHPEPVL HHRL
	Isoform 3 [UniParc]. Checksum: DF9063552B7E5E2E	FASTA	345	40,009
	10 20 30 40 50 60 MAVPPSAPQP RASFHLRRHT PCPQCSWGME EKAAASASCR EPPGPPRAAA VAYFGISVDP 70 80 90 100 110 120 DDILPGALRL IQELRPHWKP EQVRTKRFTD GITNKLVACY VEEDMQDCVL VRVYGERTEL 130 140 150 160 170 180 LVDRENEVRN FQLLRAHSCA PKLYCTFQNG LCYEYMQGVA LEPEHIREPR LFSLSADVPK 190 200 210 220 230 240 VEVLERELAW LKEHLSQLES PVVFCHNDLL CKNIIYDSIK GHVRFIDYEY AGYNYQAFDI 250 260 270 280 290 300 GNHFNEFAGV NEVDYCLYPA RETQLQWLHY YLQAQKGMAV TPREVQRLYV QVNKFALASH 310 320 330 340 FFWALWALIQ NQYSTIDFDF LRYAVIRFNQ YFKVKPQASA LEMPK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 25-386 (ISOFORM 3). Tissue: Testis.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Uterus.
[5]	"Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas." Yamada S., Ohira M., Horie H., Ando K., Takayasu H., Suzuki Y., Sugano S., Hirata T., Goto T., Matsunaga T., Hiyama E., Hayashi Y., Ando H., Suita S., Kaneko M., Sasaki F., Hashizume K., Ohnuma N., Nakagawara A. Oncogene 23:5901-5911(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 25-386 (ISOFORM 1), VARIANT GLN-227. Tissue: Hepatoblastoma.
[6]	"Overexpression of a mammalian ethanolamine-specific kinase accelerates the CDP-ethanolamine pathway." Lykidis A., Wang J., Karim M.A., Jackowski S. J. Biol. Chem. 276:2174-2179(2001) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK001623 mRNA. Translation: BAA91793.1. AK302145 mRNA. Translation: BAH13637.1. Different initiation. AL592146 Genomic DNA. Translation: CAI16602.1. AL592146 Genomic DNA. Translation: CAI16603.1. CH471067 Genomic DNA. Translation: EAW91501.1. CH471067 Genomic DNA. Translation: EAW91502.1. BC010082 mRNA. Translation: AAH10082.1. AB073608 mRNA. Translation: BAD38645.1.
IPI	IPI00019374. IPI00514040. IPI00514943.
RefSeq	NP_060678.2. NM_018208.2.
UniGene	Hs.497469.
3D structure databases
ProteinModelPortal	Q9NVF9.
SMR	Q9NVF9. Positions 62-381.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NVF9. 3 interactions.
STRING	9606.ENSP00000356170.
Polymorphism databases
DMDM	296439366.
Proteomic databases
PaxDb	Q9NVF9.
PRIDE	Q9NVF9.
Protocols and materials databases
DNASU	55224.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000367201; ENSP00000356169; ENSG00000143845. ENST00000367202; ENSP00000356170; ENSG00000143845.
GeneID	55224.
KEGG	hsa:55224.
UCSC	uc001han.4. human. uc001hao.4. human. uc010pqs.2. human.
Organism-specific databases
CTD	55224.
GeneCards	GC01M204100.
H-InvDB	HIX0001494.
HGNC	HGNC:25575. ETNK2.
HPA	HPA057167.
MIM	609859. gene.
neXtProt	NX_Q9NVF9.
PharmGKB	PA134888760.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0510.
HOGENOM	HOG000004856.
HOVERGEN	HBG018981.
InParanoid	Q9NVF9.
KO	K00894.
OMA	VPCFNIS.
OrthoDB	EOG4HT8S7.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
UniPathway	UPA00558; UER00741.
Gene expression databases
ArrayExpress	Q9NVF9.
Bgee	Q9NVF9.
CleanEx	HS_ETNK2.
Genevestigator	Q9NVF9.
GermOnline	ENSG00000143845. Homo sapiens.
Family and domain databases
InterPro	IPR026712. Cho/Etha_kinase. IPR011009. Kinase-like_dom. [Graphical view]
PANTHER	PTHR22603. PTHR22603. 1 hit.
SUPFAM	SSF56112. Kinase_like. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	ETNK2. human.
GenomeRNAi	55224.
NextBio	59212.
SOURCE	Search...

Entry information

Entry name

EKI2_HUMAN

Accession

Primary (citable) accession number: Q9NVF9
Secondary accession number(s): B7Z7K1 , Q5SXX5, Q68CK3, Q96G05

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 98 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.