Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin carboxyl-terminal hydrolase 40

Gene

USP40

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May be catalytically inactive.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei50NucleophileIEP:1
Active sitei305Proton acceptorIEP:1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Protein family/group databases

MEROPSiC19.069.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 40 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 40
Ubiquitin thioesterase 40
Ubiquitin-specific-processing protease 40
Gene namesi
Name:USP40
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000085982.13.
HGNCiHGNC:20069. USP40.

Pathology & Biotechi

Organism-specific databases

DisGeNETi55230.
OpenTargetsiENSG00000085982.
PharmGKBiPA134909324.

Polymorphism and mutation databases

BioMutaiUSP40.
DMDMi59803114.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000806701 – 1235Ubiquitin carboxyl-terminal hydrolase 40Add BLAST1235

Proteomic databases

EPDiQ9NVE5.
MaxQBiQ9NVE5.
PaxDbiQ9NVE5.
PeptideAtlasiQ9NVE5.
PRIDEiQ9NVE5.

PTM databases

iPTMnetiQ9NVE5.
PhosphoSitePlusiQ9NVE5.

Expressioni

Tissue specificityi

Broadly expressed.1 Publication

Gene expression databases

BgeeiENSG00000085982.
CleanExiHS_USP40.
ExpressionAtlasiQ9NVE5. baseline and differential.
GenevisibleiQ9NVE5. HS.

Organism-specific databases

HPAiHPA005821.

Interactioni

Protein-protein interaction databases

BioGridi120525. 1 interactor.
STRINGi9606.ENSP00000415434.

Structurei

3D structure databases

ProteinModelPortaliQ9NVE5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 482USPAdd BLAST442

Sequence similaritiesi

Belongs to the peptidase C19 family.IKR:

Phylogenomic databases

eggNOGiKOG1863. Eukaryota.
COG5077. LUCA.
GeneTreeiENSGT00760000119158.
HOGENOMiHOG000154760.
HOVERGENiHBG079302.
InParanoidiQ9NVE5.
KOiK11869.
OMAiVIIHKGG.
OrthoDBiEOG091G00MP.
PhylomeDBiQ9NVE5.
TreeFamiTF106281.

Family and domain databases

InterProiView protein in InterPro
IPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
PfamiView protein in Pfam
PF00443. UCH. 1 hit.
PROSITEiView protein in PROSITE
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NVE5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFGDLFEEEY STVSNNQYGK GKKLKTKALE PPAPREFTNL SGIRNQGGTC
60 70 80 90 100
YLNSLLQTLH FTPEFREALF SLGPEELGLF EDKDKPDAKV RIIPLQLQRL
110 120 130 140 150
FAQLLLLDQE AASTADLTDS FGWTSNEEMR QHDVQELNRI LFSALETSLV
160 170 180 190 200
GTSGHDLIYR LYHGTIVNQI VCKECKNVSE RQEDFLDLTV AVKNVSGLED
210 220 230 240 250
ALWNMYVEEE VFDCDNLYHC GTCDRLVKAA KSAKLRKLPP FLTVSLLRFN
260 270 280 290 300
FDFVKCERYK ETSCYTFPLR INLKPFCEQS ELDDLEYIYD LFSVIIHKGG
310 320 330 340 350
CYGGHYHVYI KDVDHLGNWQ FQEEKSKPDV NLKDLQSEEE IDHPLMILKA
360 370 380 390 400
ILLEENNLIP VDQLGQKLLK KIGISWNKKY RKQHGPLRKF LQLHSQIFLL
410 420 430 440 450
SSDESTVRLL KNSSLQAESD FQRNDQQIFK MLPPESPGLN NSISCPHWFD
460 470 480 490 500
INDSKVQPIR EKDIEQQFQG KESAYMLFYR KSQLQRPPEA RANPRYGVPC
510 520 530 540 550
HLLNEMDAAN IELQTKRAEC DSANNTFELH LHLGPQYHFF NGALHPVVSQ
560 570 580 590 600
TESVWDLTFD KRKTLGDLRQ SIFQLLEFWE GDMVLSVAKL VPAGLHIYQS
610 620 630 640 650
LGGDELTLCE TEIADGEDIF VWNGVEVGGV HIQTGIDCEP LLLNVLHLDT
660 670 680 690 700
SSDGEKCCQV IESPHVFPAN AEVGTVLTAL AIPAGVIFIN SAGCPGGEGW
710 720 730 740 750
TAIPKEDMRK TFREQGLRNG SSILIQDSHD DNSLLTKEEK WVTSMNEIDW
760 770 780 790 800
LHVKNLCQLE SEEKQVKISA TVNTMVFDIR IKAIKELKLM KELADNSCLR
810 820 830 840 850
PIDRNGKLLC PVPDSYTLKE AELKMGSSLG LCLGKAPSSS QLFLFFAMGS
860 870 880 890 900
DVQPGTEMEI VVEETISVRD CLKLMLKKSG LQGDAWHLRK MDWCYEAGEP
910 920 930 940 950
LCEEDATLKE LLICSGDTLL LIEGQLPPLG FLKVPIWWYQ LQGPSGHWES
960 970 980 990 1000
HQDQTNCTSS WGRVWRATSS QGASGNEPAQ VSLLYLGDIE ISEDATLAEL
1010 1020 1030 1040 1050
KSQAMTLPPF LEFGVPSPAH LRAWTVERKR PGRLLRTDRQ PLREYKLGRR
1060 1070 1080 1090 1100
IEICLEPLQK GENLGPQDVL LRTQVRIPGE RTYAPALDLV WNAAQGGTAG
1110 1120 1130 1140 1150
SLRQRVADFY RLPVEKIEIA KYFPEKFEWL PISSWNQQIT KRKKKKKQDY
1160 1170 1180 1190 1200
LQGAPYYLKD GDTIGVKNLL IDDDDDFSTI RDDTGKEKQK QRALGRRKSQ
1210 1220 1230
EALHEQSSYI LSSAETPARP RAPETSLSIH VGSFR
Length:1,235
Mass (Da):140,130
Last modified:February 15, 2005 - v3
Checksum:iBEB20702566D39A8
GO
Isoform 2 (identifier: Q9NVE5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-824: Missing.
     973-987: ASGNEPAQVSLLYLG → ENRMGFQQPVHHKEK
     988-1235: Missing.

Note: No experimental confirmation available.
Show »
Length:163
Mass (Da):18,298
Checksum:i6DFD238BB2810182
GO
Isoform 3 (identifier: Q9NVE5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MSLFLRVVFSFTM

Show »
Length:1,247
Mass (Da):141,558
Checksum:iEB6B19218247C3FB
GO

Sequence cautioni

The sequence BAA91807 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.IKR:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti277 – 318CEQSE…DHLGN → FFSFNQKMHNVCVNRRYGGS GMPLLRCGRCVGSAQPLSSV FR in AAH67300 (PubMed:15489334).IKR:Add BLAST42

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_017123666V → A. Corresponds to variant dbSNP:rs838543Ensembl.1
Natural variantiVAR_0597531025T → M. Corresponds to variant dbSNP:rs34026756Ensembl.1
Natural variantiVAR_0171241111R → C. Corresponds to variant dbSNP:rs1048603Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0085951 – 824Missing in isoform 2. IBD:Add BLAST824
Alternative sequenceiVSP_0409381M → MSLFLRVVFSFTM in isoform 3. IBD:1
Alternative sequenceiVSP_008596973 – 987ASGNE…LLYLG → ENRMGFQQPVHHKEK in isoform 2. IBD:Add BLAST15
Alternative sequenceiVSP_012819988 – 1235Missing in isoform 2. IBD:Add BLAST248

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ583821 mRNA. Translation: CAE47748.2.
AK001647 mRNA. Translation: BAA91807.1. Different initiation.
AK124094 mRNA. Translation: BAC85770.1.
AC019221 Genomic DNA. No translation available.
AC019072 Genomic DNA. No translation available.
BC067300 mRNA. Translation: AAH67300.1.
CCDSiCCDS46547.1. [Q9NVE5-3]
RefSeqiNP_060688.1. NM_018218.2. [Q9NVE5-3]
UniGeneiHs.96513.

Genome annotation databases

EnsembliENST00000251722; ENSP00000251722; ENSG00000085982. [Q9NVE5-1]
ENST00000409945; ENSP00000386664; ENSG00000085982. [Q9NVE5-2]
ENST00000427112; ENSP00000387898; ENSG00000085982. [Q9NVE5-1]
ENST00000450966; ENSP00000415434; ENSG00000085982. [Q9NVE5-3]
GeneIDi55230.
KEGGihsa:55230.
UCSCiuc002vuo.1. human. [Q9NVE5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiUBP40_HUMAN
AccessioniPrimary (citable) accession number: Q9NVE5
Secondary accession number(s): Q6NX38, Q70EL0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: February 15, 2005
Last modified: November 22, 2017
This is version 134 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families