Q9NV66 (TYW1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA wybutosine-synthesizing protein 1 homolog

Alternative name(s):
Radical S-adenosyl methionine and flavodoxin domain-containing protein 1
tRNA-yW-synthesizing protein

Gene names

Name:	TYW1
Synonyms:	RSAFD1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	732 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probable component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA By similarity.
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Pathway	tRNA modification; wybutosine-tRNA(Phe) biosynthesis.
Sequence similarities	Belongs to the TYW1 family. Contains 1 flavodoxin-like domain.
Sequence caution	The sequence AAH15591.1 differs from that shown. Reason: Frameshift at position 663. The sequence BAB14307.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	tRNA processing
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Nucleotide-binding S-adenosyl-L-methionine
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FMN binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NV66-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NV66-2)

The sequence of this isoform differs from the canonical sequence as follows:
     368-384: GYQLIGSHSGVKLCRWT → VHAPRERRLLQTHILWN
     385-732: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 732	732	tRNA wybutosine-synthesizing protein 1 homolog		PRO_0000281826
Regions
	Domain	79 – 237	159	Flavodoxin-like
	Nucleotide binding	85 – 89	5	FMN By similarity
	Nucleotide binding	176 – 208	33	FMN By similarity
Sites
	Metal binding	416	1	Iron-sulfur (4Fe-4S-S-AdoMet) Potential
	Metal binding	420	1	Iron-sulfur (4Fe-4S-S-AdoMet) Potential
	Metal binding	423	1	Iron-sulfur (4Fe-4S-S-AdoMet) Potential
Natural variations
	Alternative sequence	368 – 384	17	GYQLI…LCRWT → VHAPRERRLLQTHILWN in isoform 2.		VSP_024066
	Alternative sequence	385 – 732	348	Missing in isoform 2.		VSP_024067
	Natural variant	462	1	G → V. Corresponds to variant rs2261015 [ dbSNP | Ensembl ].		VAR_031288
	Natural variant	632	1	H → R. Ref.1 Corresponds to variant rs2949097 [ dbSNP | Ensembl ].		VAR_031289
	Natural variant	671	1	D → N. Ref.1 Corresponds to variant rs28450001 [ dbSNP | Ensembl ].		VAR_031290
Experimental info
	Sequence conflict	382	1	R → M in BAB14307. Ref.1
	Sequence conflict	388	1	L → F in BAB14307. Ref.1
	Sequence conflict	416	1	C → Y in BAB14307. Ref.1
	Sequence conflict	543	1	K → R in BAB14307. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 3, 2007. Version 2. Checksum: 95E8E5C3F88ADD23	FASTA	732	83,702
	10 20 30 40 50 60 MDPSADTWDL FSPLISLWIN RFYIYLGFAV SISLWICVQI VIKTQGKNLQ EKSVPKAAQD 70 80 90 100 110 120 LMTNGYVSLQ EKDIFVSGVK IFYGSQTGTA KGFATVLAEA VTSLDLPVAI INLKEYDPDD 130 140 150 160 170 180 HLIEEVTSKN VCVFLVATYT DGLPTESAEW FCKWLEEASI DFRFGKTYLK GMRYAVFGLG 190 200 210 220 230 240 NSAYASHFNK VGKNVDKWLW MLGAHRVMSR GEGDCDVVKS KHGSIEADFR AWKTKFISQL 250 260 270 280 290 300 QALQKGERKK SCGGHCKKGK CESHQHGSEE REEGSHEQDE LHHRDTEEEE PFESSSEEEF 310 320 330 340 350 360 GGEDHQSLNS IVDVEDLGKI MDHVKKEKRE KEQQEEKSGL FRNMGRNEDG ERRAMITPAL 370 380 390 400 410 420 REALTKQGYQ LIGSHSGVKL CRWTKSMLRG RGGCYKHTFY GIESHRCMET TPSLACANKC 430 440 450 460 470 480 VFCWRHHTNP VGTEWRWKMD QPEMILKEAI ENHQNMIKQF KGVPGVKAER FEEGMTVKHC 490 500 510 520 530 540 ALSLVGEPIM YPEINRFLKL LHQCKISSFL VTNAQFPAEI RNLEPVTQLY VSVDASTKDS 550 560 570 580 590 600 LKKIDRPLFK DFWQRFLDSL KALAVKQQRT VYRLTLVKAW NVDELQAYAQ LVSLGNPDFI 610 620 630 640 650 660 EVKGVTYCGE SSASSLTMAH VPWHEEVVQF VHELVDLIPE YEIACEHEHS NCLLIAHRKF 670 680 690 700 710 720 KIGGEWWTWI DYNRFQELIQ EYEDSGGSKT FSAKDYMART PHWALFGASE RGFDPKDTRH 730 QRKNKSKAIS GC
	Isoform 2 [UniParc]. Checksum: 43A06B3E5DFC86C6	FASTA	384	43,700
	10 20 30 40 50 60 MDPSADTWDL FSPLISLWIN RFYIYLGFAV SISLWICVQI VIKTQGKNLQ EKSVPKAAQD 70 80 90 100 110 120 LMTNGYVSLQ EKDIFVSGVK IFYGSQTGTA KGFATVLAEA VTSLDLPVAI INLKEYDPDD 130 140 150 160 170 180 HLIEEVTSKN VCVFLVATYT DGLPTESAEW FCKWLEEASI DFRFGKTYLK GMRYAVFGLG 190 200 210 220 230 240 NSAYASHFNK VGKNVDKWLW MLGAHRVMSR GEGDCDVVKS KHGSIEADFR AWKTKFISQL 250 260 270 280 290 300 QALQKGERKK SCGGHCKKGK CESHQHGSEE REEGSHEQDE LHHRDTEEEE PFESSSEEEF 310 320 330 340 350 360 GGEDHQSLNS IVDVEDLGKI MDHVKKEKRE KEQQEEKSGL FRNMGRNEDG ERRAMITPAL 370 380 REALTKQVHA PRERRLLQTH ILWN

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS ARG-632 AND ASN-671. Tissue: Teratocarcinoma.
[2]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Colon, Placenta and Uterus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK001762 mRNA. Translation: BAA91891.1. AK022917 mRNA. Translation: BAB14307.1. Different initiation. AC079920 Genomic DNA. No translation available. AC006480 Genomic DNA. Translation: AAS07568.1. AC073089 Genomic DNA. Translation: AAS07520.1. BC015591 mRNA. Translation: AAH15591.1. Frameshift. BC042156 mRNA. Translation: AAH42156.1. Different termination. BC051888 mRNA. Translation: AAH51888.1.
IPI	IPI00182304. IPI00844116.
RefSeq	NP_060734.2. NM_018264.3.
UniGene	Hs.520917.
3D structure databases
HSSP	HSSP built from PDB template 1TLL based on UniProtKB P29476.
ProteinModelPortal	Q9NV66.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NV66. 1 interaction.
STRING	9606.ENSP00000352645.
PTM databases
PhosphoSite	Q9NV66.
Polymorphism databases
DMDM	143678193.
Proteomic databases
PaxDb	Q9NV66.
PRIDE	Q9NV66.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000359626; ENSP00000352645; ENSG00000198874. ENST00000361660; ENSP00000354795; ENSG00000198874.
GeneID	55253.
KEGG	hsa:55253.
UCSC	uc003tvn.3. human.
Organism-specific databases
CTD	55253.
GeneCards	GC07P066460.
H-InvDB	HIX0006729.
HGNC	HGNC:25598. TYW1.
HPA	HPA015535.
MIM	611243. gene.
neXtProt	NX_Q9NV66.
PharmGKB	PA134940167.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0731.
HOVERGEN	HBG108623.
InParanoid	Q9NV66.
KO	K15449.
OMA	ERREMIT.
OrthoDB	EOG4548Z4.
PhylomeDB	Q9NV66.
Enzyme and pathway databases
UniPathway	UPA00375.
Gene expression databases
ArrayExpress	Q9NV66.
Bgee	Q9NV66.
CleanEx	HS_TYW1.
Genevestigator	Q9NV66.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR001094. Flavdoxin. IPR008254. Flavodoxin/NO_synth. IPR007197. rSAM. IPR013917. tRNA_wybutosine-synth. [Graphical view]
Pfam	PF00258. Flavodoxin_1. 1 hit. PF04055. Radical_SAM. 1 hit. PF08608. Wyosine_form. 1 hit. [Graphical view]
PRINTS	PR00369. FLAVODOXIN.
PROSITE	PS50902. FLAVODOXIN_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	55253.
NextBio	59324.
SOURCE	Search...

Entry information

Entry name

TYW1_HUMAN

Accession

Primary (citable) accession number: Q9NV66
Secondary accession number(s): Q6PJG8 , Q75MG8, Q75MN3, Q86V12, Q8IVS7, Q9H9C4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	April 3, 2007
Last modified:	May 1, 2013
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.