Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q9NV23 (SAST_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
S-acyl fatty acid synthase thioesterase, medium chain

EC=3.1.2.14
Alternative name(s):
Augmented in rheumatoid arthritis 1
Short name=AURA1
Oleoyl-ACP hydrolase
Thioesterase II
Thioesterase domain-containing protein 1
Gene names
Name:OLAH
Synonyms:THEDC1
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length265 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

In fatty acid biosynthesis chain termination and release of the free fatty acid product is achieved by hydrolysis of the thio ester by a thioesterase I, a component of the fatty acid synthetase complex. The chain length of the released fatty acid is usually C16. However, in the mammary glands of non-ruminant mammals, and in the uropygial gland of certain waterfowl there exists a second thioesterase which releases medium-chain length fatty acids (C8 to C2) By similarity.

Catalytic activity

Oleoyl-[acyl-carrier-protein] + H2O = [acyl-carrier-protein] + oleate.

Tissue specificity

Isoform 2: Up-regulated in bone marrow-derived mononuclear cells of rheumatoid arthritis patients. Ref.5

Sequence similarities

Belongs to the thioesterase family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9NV23-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9NV23-2)

The sequence of this isoform differs from the canonical sequence as follows:
     55-55: V → ETASHHVAKAGLKLRRSSDPPASAYPCAGVSHRRREPPCLAKILGLFWILIFFM

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 265265S-acyl fatty acid synthase thioesterase, medium chain
PRO_0000180358

Sites

Active site1011 By similarity
Active site2371 By similarity

Natural variations

Alternative sequence551V → ETASHHVAKAGLKLRRSSDP PASAYPCAGVSHRRREPPCL AKILGLFWILIFFM in isoform 2.
VSP_040022

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: C67722F3CAD2D2C7

FASTA26529,931
        10         20         30         40         50         60 
MERGDQPKRT RNENIFNCLY KNPEATFKLI CFPWMGGGST HFAKWGQDTH DLLEVHSLRL 

        70         80         90        100        110        120 
PGRESRVEEP LENDISQLVD EVVCALQPVI QDKPFAFFGH SMGSYIAFRT ALGLKENNQP 

       130        140        150        160        170        180 
EPLHLFLSSA TPVHSKAWHR IPKDDELSEE QISHYLMEFG GTPKHFAEAK EFVKQCSPII 

       190        200        210        220        230        240 
RADLNIVRSC TSNVPSKAVL SCDLTCFVGS EDIAKDMEAW KDVTSGNAKI YQLPGGHFYL 

       250        260 
LDPANEKLIK NYIIKCLEVS SISNF 

« Hide

Isoform 2 [UniParc].

Checksum: C7DB0DFEBCB0823F
Show »

FASTA31835,818

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Placenta.
[2]"The DNA sequence and comparative analysis of human chromosome 10."
Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. expand/collapse author list , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
Nature 429:375-381(2004) [PubMed: 15164054] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[5]"Isolation and expression profiling of genes upregulated in bone marrow-derived mononuclear cells of rheumatoid arthritis patients."
Nakamura N., Shimaoka Y., Tougan T., Onda H., Okuzaki D., Zhao H., Fujimori A., Yabuta N., Nagamori I., Tanigawa A., Sato J., Oda T., Hayashida K., Suzuki R., Yukioka M., Nojima H., Ochi T.
DNA Res. 13:169-183(2006) [PubMed: 17082220] [Abstract]
Cited for: EXPRESSION AND UP-REGULATION OF ISOFORM 2, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK001844 mRNA. Translation: BAA91937.1.
AK001968 mRNA. Translation: BAA92007.1.
AL590365 Genomic DNA. Translation: CAH73961.1.
AL590365 Genomic DNA. Translation: CAH73962.1.
CH471072 Genomic DNA. Translation: EAW86244.1.
CH471072 Genomic DNA. Translation: EAW86245.1.
BC050372 mRNA. Translation: AAH50372.1.
BR000403 mRNA. Translation: FAA00320.1.
IPIIPI00019562.
IPI00030167.
RefSeqNP_001034791.1. NM_001039702.2.
NP_060794.1. NM_018324.2.
UniGeneHs.24309.

3D structure databases

ProteinModelPortalQ9NV23.
SMRQ9NV23. Positions 10-257.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9NV23.

Polymorphism databases

DMDM52783423.

Proteomic databases

PRIDEQ9NV23.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000378228; ENSP00000367473; ENSG00000152463.
GeneID55301.
KEGGhsa:55301.
UCSCuc001inu.1. human.

Organism-specific databases

CTD55301.
GeneCardsGC10P015074.
HGNCHGNC:25625. OLAH.
HPAHPA037947.
HPA037948.
neXtProtNX_Q9NV23.
PharmGKBPA134955731.
GenAtlasSearch...

Phylogenomic databases

GeneTreeENSGT00390000015518.
HOVERGENHBG015455.
OMASCDLTCF.
PhylomeDBQ9NV23.

Gene expression databases

ArrayExpressQ9NV23.
BgeeQ9NV23.
CleanExHS_OLAH.
GenevestigatorQ9NV23.
GermOnlineENSG00000152463. Homo sapiens.

Family and domain databases

InterProIPR012223. TEII.
IPR001031. Thioesterase.
[Graphical view]
KOK01071.
PANTHERPTHR11487. TEII. 1 hit.
PfamPF00975. Thioesterase. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio59524.

Entry information

Entry nameSAST_HUMAN
AccessionPrimary (citable) accession number: Q9NV23
Secondary accession number(s): Q5VUB6, Q9NUW1
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: October 1, 2000
Last modified: January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM

SIMILARITY comments

Index of protein domains and families