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Protein

ATP-dependent RNA helicase DDX19A

Gene

DDX19A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase involved in mRNA export from the nucleus. Rather than unwinding RNA duplexes, DDX19 functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118ATPBy similarity1
Binding sitei428ATPBy similarity1
Binding sitei431ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi137 – 144ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000168872-MONOMER.

Protein family/group databases

TCDBi1.I.1.1.3. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DDX19A (EC:3.6.4.13)
Alternative name(s):
DDX19-like protein
DEAD box protein 19A
Gene namesi
Name:DDX19A
Synonyms:DDX19L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:25628. DDX19A.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • membrane Source: UniProtKB
  • nuclear membrane Source: UniProtKB-SubCell
  • nuclear pore Source: UniProtKB-SubCell
  • nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nuclear pore complex, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi55308.
OpenTargetsiENSG00000168872.
PharmGKBiPA134894996.

Polymorphism and mutation databases

BioMutaiDDX19A.
DMDMi73919226.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000550212 – 478ATP-dependent RNA helicase DDX19AAdd BLAST477

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Cross-linki26Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)Combined sources
Cross-linki26Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ9NUU7.
MaxQBiQ9NUU7.
PaxDbiQ9NUU7.
PeptideAtlasiQ9NUU7.
PRIDEiQ9NUU7.

PTM databases

iPTMnetiQ9NUU7.
PhosphoSitePlusiQ9NUU7.

Expressioni

Gene expression databases

BgeeiENSG00000168872.
CleanExiHS_DDX19A.
ExpressionAtlasiQ9NUU7. baseline and differential.
GenevisibleiQ9NUU7. HS.

Organism-specific databases

HPAiHPA045252.
HPA066668.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
MIF4GDA9UHW65EBI-740301,EBI-373498

Protein-protein interaction databases

BioGridi120592. 26 interactors.
IntActiQ9NUU7. 8 interactors.
MINTiMINT-1450935.
STRINGi9606.ENSP00000306117.

Structurei

3D structure databases

ProteinModelPortaliQ9NUU7.
SMRiQ9NUU7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini124 – 294Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST171
Domaini305 – 473Helicase C-terminalPROSITE-ProRule annotationAdd BLAST169

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 299N-terminal lobeBy similarityAdd BLAST298
Regioni54 – 67N-terminal helixBy similarityAdd BLAST14
Regioni300 – 478C-terminal lobeBy similarityAdd BLAST179

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi91 – 119Q motifAdd BLAST29
Motifi241 – 244DEAD box4

Domaini

The N-terminal extension helix acts as an autoinhibitory domain, preventing ATP hydrolysis, unless the N-terminus of the protein is displaced by RNA binding, allowing cleft closure to bring key side chains into position for catalysis.By similarity

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0332. Eukaryota.
ENOG410XRGX. LUCA.
GeneTreeiENSGT00530000063236.
HOGENOMiHOG000268797.
HOVERGENiHBG107989.
InParanoidiQ9NUU7.
KOiK18655.
OMAiQAICISP.
PhylomeDBiQ9NUU7.
TreeFamiTF314957.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NUU7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATDSWALAV DEQEAAVKSM TNLQIKEEKV KADTNGIIKT STTAEKTDEE
60 70 80 90 100
EKEDRAAQSL LNKLIRSNLV DNTNQVEVLQ RDPNSPLYSV KSFEELRLKP
110 120 130 140 150
QLLQGVYAMG FNRPSKIQEN ALPMMLAEPP QNLIAQSQSG TGKTAAFVLA
160 170 180 190 200
MLSRVEPSDR YPQCLCLSPT YELALQTGKV IEQMGKFYPE LKLAYAVRGN
210 220 230 240 250
KLERGQKISE QIVIGTPGTV LDWCSKLKFI DPKKIKVFVL DEADVMIATQ
260 270 280 290 300
GHQDQSIRIQ RMLPRNCQML LFSATFEDSV WKFAQKVVPD PNVIKLKREE
310 320 330 340 350
ETLDTIKQYY VLCSSRDEKF QALCNLYGAI TIAQAMIFCH TRKTASWLAA
360 370 380 390 400
ELSKEGHQVA LLSGEMMVEQ RAAVIERFRE GKEKVLVTTN VCARGIDVEQ
410 420 430 440 450
VSVVINFDLP VDKDGNPDNE TYLHRIGRTG RFGKRGLAVN MVDSKHSMNI
460 470
LNRIQEHFNK KIERLDTDDL DEIEKIAN
Length:478
Mass (Da):53,975
Last modified:October 1, 2000 - v1
Checksum:iC51F9E1D7B8584D0
GO
Isoform 2 (identifier: Q9NUU7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-98: MATDSWALAV...SVKSFEELRL → MSGTFLIR

Note: No experimental confirmation available.
Show »
Length:388
Mass (Da):43,875
Checksum:i71A5EA464F65A911
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti463E → G in BAD96982 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0569541 – 98MATDS…EELRL → MSGTFLIR in isoform 2. 1 PublicationAdd BLAST98

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001988 mRNA. Translation: BAA92022.1.
AK299504 mRNA. Translation: BAG61459.1.
AK223262 mRNA. Translation: BAD96982.1.
AL832970 mRNA. Translation: CAH10622.1.
AC012184 Genomic DNA. No translation available.
CH471241 Genomic DNA. Translation: EAW51826.1.
BC005162 mRNA. Translation: AAH05162.1.
BC006544 mRNA. Translation: AAH06544.1.
BC137496 mRNA. Translation: AAI37497.1.
BC137497 mRNA. Translation: AAI37498.1.
CCDSiCCDS10889.1. [Q9NUU7-1]
RefSeqiNP_001307451.1. NM_001320522.1.
NP_001307454.1. NM_001320525.1. [Q9NUU7-2]
NP_001307455.1. NM_001320526.1.
NP_001307456.1. NM_001320527.1.
NP_060802.1. NM_018332.4. [Q9NUU7-1]
UniGeneiHs.656037.

Genome annotation databases

EnsembliENST00000302243; ENSP00000306117; ENSG00000168872. [Q9NUU7-1]
GeneIDi55308.
KEGGihsa:55308.
UCSCiuc002eyv.4. human. [Q9NUU7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001988 mRNA. Translation: BAA92022.1.
AK299504 mRNA. Translation: BAG61459.1.
AK223262 mRNA. Translation: BAD96982.1.
AL832970 mRNA. Translation: CAH10622.1.
AC012184 Genomic DNA. No translation available.
CH471241 Genomic DNA. Translation: EAW51826.1.
BC005162 mRNA. Translation: AAH05162.1.
BC006544 mRNA. Translation: AAH06544.1.
BC137496 mRNA. Translation: AAI37497.1.
BC137497 mRNA. Translation: AAI37498.1.
CCDSiCCDS10889.1. [Q9NUU7-1]
RefSeqiNP_001307451.1. NM_001320522.1.
NP_001307454.1. NM_001320525.1. [Q9NUU7-2]
NP_001307455.1. NM_001320526.1.
NP_001307456.1. NM_001320527.1.
NP_060802.1. NM_018332.4. [Q9NUU7-1]
UniGeneiHs.656037.

3D structure databases

ProteinModelPortaliQ9NUU7.
SMRiQ9NUU7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120592. 26 interactors.
IntActiQ9NUU7. 8 interactors.
MINTiMINT-1450935.
STRINGi9606.ENSP00000306117.

Protein family/group databases

TCDBi1.I.1.1.3. the nuclear pore complex (npc) family.

PTM databases

iPTMnetiQ9NUU7.
PhosphoSitePlusiQ9NUU7.

Polymorphism and mutation databases

BioMutaiDDX19A.
DMDMi73919226.

Proteomic databases

EPDiQ9NUU7.
MaxQBiQ9NUU7.
PaxDbiQ9NUU7.
PeptideAtlasiQ9NUU7.
PRIDEiQ9NUU7.

Protocols and materials databases

DNASUi55308.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000302243; ENSP00000306117; ENSG00000168872. [Q9NUU7-1]
GeneIDi55308.
KEGGihsa:55308.
UCSCiuc002eyv.4. human. [Q9NUU7-1]

Organism-specific databases

CTDi55308.
DisGeNETi55308.
GeneCardsiDDX19A.
H-InvDBHIX0038837.
HGNCiHGNC:25628. DDX19A.
HPAiHPA045252.
HPA066668.
neXtProtiNX_Q9NUU7.
OpenTargetsiENSG00000168872.
PharmGKBiPA134894996.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0332. Eukaryota.
ENOG410XRGX. LUCA.
GeneTreeiENSGT00530000063236.
HOGENOMiHOG000268797.
HOVERGENiHBG107989.
InParanoidiQ9NUU7.
KOiK18655.
OMAiQAICISP.
PhylomeDBiQ9NUU7.
TreeFamiTF314957.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000168872-MONOMER.

Miscellaneous databases

GenomeRNAii55308.
PROiQ9NUU7.

Gene expression databases

BgeeiENSG00000168872.
CleanExiHS_DDX19A.
ExpressionAtlasiQ9NUU7. baseline and differential.
GenevisibleiQ9NUU7. HS.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDD19A_HUMAN
AccessioniPrimary (citable) accession number: Q9NUU7
Secondary accession number(s): B2RPL0, B4DRZ7, Q53FM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.