Q9NUN7 (ACER3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alkaline ceramidase 3
Short name=AlkCDase 3
Short name=Alkaline CDase 3
EC=3.5.1.-

Alternative name(s):
Alkaline dihydroceramidase SB89
Alkaline phytoceramidase
Short name=aPHC

Gene names

Name:	ACER3
Synonyms:	APHC, PHCA

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity.
Enzyme regulation	Activated by Ca2+ and inhibited by Zn2+.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein.
Tissue specificity	Ubiquitously expressed. Highest expression in placenta.
Sequence similarities	Belongs to the alkaline ceramidase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ceramide metabolic process Inferred from electronic annotation. Source: InterPro phytosphingosine biosynthetic process Inferred from direct assay Ref.1PubMed 20068046. Source: UniProtKB positive regulation of cell proliferation Inferred from mutant phenotype PubMed 20068046. Source: UniProtKB sphingosine biosynthetic process Inferred from direct assay PubMed 20068046. Source: UniProtKB
Cellular_component	integral to Golgi membrane Inferred from direct assay Ref.1. Source: UniProtKB integral to endoplasmic reticulum membrane Inferred from direct assay Ref.1. Source: UniProtKB
Molecular_function	phytoceramidase activity Inferred from direct assay Ref.1PubMed 20068046. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NUN7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NUN7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-133: Missing.
     134-145: TVYLKVKEPIFH → MAQSRLIGTSTS

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Alkaline ceramidase 3		PRO_0000212463
Regions
	Transmembrane	29 – 51	23	Helical; Potential
	Transmembrane	63 – 84	22	Helical; Potential
	Transmembrane	94 – 111	18	Helical; Potential
	Transmembrane	118 – 137	20	Helical; Potential
	Transmembrane	147 – 169	23	Helical; Potential
	Transmembrane	174 – 196	23	Helical; Potential
	Transmembrane	216 – 238	23	Helical; Potential
Amino acid modifications
	Glycosylation	24	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 133	133	Missing in isoform 2.		VSP_039162
	Alternative sequence	134 – 145	12	TVYLK…EPIFH → MAQSRLIGTSTS in isoform 2.		VSP_039163
Experimental info
	Sequence conflict	52	1	V → I in AAK71923. Ref.1
	Sequence conflict	52	1	V → I in AAL56013. Ref.2
	Sequence conflict	52	1	V → I in BAG37496. Ref.3
	Sequence conflict	52	1	V → I in EAW75010. Ref.5
	Sequence conflict	52	1	V → I in AAH73853. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 18, 2010. Version 3. Checksum: CFD2A901F12A5918	FASTA	267	31,552
	10 20 30 40 50 60 MAPAADREGY WGPTTSTLDW CEENYSVTWY IAEFWNTVSN LIMIIPPMFG AVQSVRDGLE 70 80 90 100 110 120 KRYIASYLAL TVVGMGSWCF HMTLKYEMQL LDELPMIYSC CIFVYCMFEC FKIKNSVNYH 130 140 150 160 170 180 LLFTLVLFSL IVTTVYLKVK EPIFHQVMYG MLVFTLVLRS IYIVTWVYPW LRGLGYTSLG 190 200 210 220 230 240 IFLLGFLFWN IDNIFCESLR NFRKKVPPII GITTQFHAWW HILTGLGSYL HILFSLYTRT 250 260 LYLRYRPKVK FLFGIWPVIL FEPLRKH
	Isoform 2 [UniParc]. Checksum: 8333C599D48A5525	FASTA	134	15,895
	10 20 30 40 50 60 MAQSRLIGTS TSQVMYGMLV FTLVLRSIYI VTWVYPWLRG LGYTSLGIFL LGFLFWNIDN 70 80 90 100 110 120 IFCESLRNFR KKVPPIIGIT TQFHAWWHIL TGLGSYLHIL FSLYTRTLYL RYRPKVKFLF 130 GIWPVILFEP LRKH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a novel human alkaline ceramidase. A mammalian enzyme that hydrolyzes phytoceramide." Mao C., Xu R., Szulc Z.M., Bielawska A., Galadari S.H., Obeid L.M. J. Biol. Chem. 276:26577-26588(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION. Tissue: Kidney.
[2]	Li N., Zhang W., Wan T., Chen T., Cao X. Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Placenta and Thalamus.
[4]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Eye.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF214454 mRNA. Translation: AAK71923.1. AF327353 mRNA. Translation: AAL56013.1. AK002100 mRNA. Translation: BAA92085.1. AK315000 mRNA. Translation: BAG37496.1. AP000752 Genomic DNA. No translation available. AP002498 Genomic DNA. No translation available. AP003119 Genomic DNA. No translation available. CH471076 Genomic DNA. Translation: EAW75010.1. BC073853 mRNA. Translation: AAH73853.1.
IPI	IPI00018961. IPI00965568.
RefSeq	NP_060837.3. NM_018367.5.
UniGene	Hs.23862.
3D structure databases
ProteinModelPortal	Q9NUN7.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NUN7. 1 interaction.
STRING	9606.ENSP00000278544.
PTM databases
PhosphoSite	Q9NUN7.
Polymorphism databases
DMDM	296439452.
Proteomic databases
PaxDb	Q9NUN7.
PRIDE	Q9NUN7.
Protocols and materials databases
DNASU	55331.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000532485; ENSP00000434480; ENSG00000078124. ENST00000544113; ENSP00000440663; ENSG00000078124. ENST00000571623; ENSP00000459570; ENSG00000262970.
GeneID	55331.
KEGG	hsa:55331.
UCSC	uc009yum.1. human.
Organism-specific databases
CTD	55331.
GeneCards	GC11P076571.
H-InvDB	HIX0021493.
HGNC	HGNC:16066. ACER3.
neXtProt	NX_Q9NUN7.
PharmGKB	PA33256.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG250726.
HOGENOM	HOG000192011.
InParanoid	Q9NUN7.
KO	K04711.
OMA	FWNIDNI.
OrthoDB	EOG4S1T7P.
PhylomeDB	Q9NUN7.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	Q9NUN7.
Bgee	Q9NUN7.
CleanEx	HS_ACER3.
Genevestigator	Q9NUN7.
GermOnline	ENSG00000078124. Homo sapiens.
Family and domain databases
InterPro	IPR008901. Ceramidase. [Graphical view]
Pfam	PF05875. Ceramidase. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	55331.
NextBio	59622.

Entry information

Entry name

ACER3_HUMAN

Accession

Primary (citable) accession number: Q9NUN7
Secondary accession number(s): B2RC99

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 14, 2001
Last sequence update:	May 18, 2010
Last modified:	May 29, 2013
This is version 102 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.