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Protein

Alkaline ceramidase 3

Gene

ACER3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity.

Enzyme regulationi

Activated by Ca2+ and inhibited by Zn2+.

GO - Molecular functioni

  • phytoceramidase activity Source: UniProtKB

GO - Biological processi

  • ceramide metabolic process Source: InterPro
  • phytosphingosine biosynthetic process Source: UniProtKB
  • positive regulation of cell proliferation Source: UniProtKB
  • sphingolipid biosynthetic process Source: Reactome
  • sphingosine biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciZFISH:ENSG00000078124-MONOMER.
BRENDAi3.5.1.23. 2681.
ReactomeiR-HSA-1660661. Sphingolipid de novo biosynthesis.

Chemistry databases

SwissLipidsiSLP:000000680.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline ceramidase 3 (EC:3.5.1.-)
Short name:
AlkCDase 3
Short name:
Alkaline CDase 3
Alternative name(s):
Alkaline dihydroceramidase SB89
Alkaline phytoceramidase
Short name:
aPHC
Gene namesi
Name:ACER3
Synonyms:APHC, PHCA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:16066. ACER3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei29 – 51HelicalSequence analysisAdd BLAST23
Transmembranei63 – 84HelicalSequence analysisAdd BLAST22
Transmembranei94 – 111HelicalSequence analysisAdd BLAST18
Transmembranei118 – 137HelicalSequence analysisAdd BLAST20
Transmembranei147 – 169HelicalSequence analysisAdd BLAST23
Transmembranei174 – 196HelicalSequence analysisAdd BLAST23
Transmembranei216 – 238HelicalSequence analysisAdd BLAST23

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: Reactome
  • integral component of endoplasmic reticulum membrane Source: UniProtKB
  • integral component of Golgi membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi55331.
OpenTargetsiENSG00000078124.
PharmGKBiPA33256.

Polymorphism and mutation databases

BioMutaiACER3.
DMDMi296439452.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002124631 – 267Alkaline ceramidase 3Add BLAST267

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi24N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9NUN7.
PeptideAtlasiQ9NUN7.
PRIDEiQ9NUN7.
TopDownProteomicsiQ9NUN7-1. [Q9NUN7-1]

PTM databases

iPTMnetiQ9NUN7.
PhosphoSitePlusiQ9NUN7.

Expressioni

Tissue specificityi

Ubiquitously expressed. Highest expression in placenta.

Gene expression databases

BgeeiENSG00000078124.
CleanExiHS_ACER3.
ExpressionAtlasiQ9NUN7. baseline and differential.
GenevisibleiQ9NUN7. HS.

Interactioni

Protein-protein interaction databases

BioGridi120612. 2 interactors.
IntActiQ9NUN7. 1 interactor.
STRINGi9606.ENSP00000434480.

Structurei

3D structure databases

ProteinModelPortaliQ9NUN7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline ceramidase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2329. Eukaryota.
ENOG4111RPN. LUCA.
GeneTreeiENSGT00730000111189.
HOGENOMiHOG000192011.
InParanoidiQ9NUN7.
KOiK04711.
OMAiVYIVLWV.
OrthoDBiEOG091G0DCZ.
PhylomeDBiQ9NUN7.
TreeFamiTF313019.

Family and domain databases

InterProiIPR008901. Ceramidase.
[Graphical view]
PfamiPF05875. Ceramidase. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NUN7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPAADREGY WGPTTSTLDW CEENYSVTWY IAEFWNTVSN LIMIIPPMFG
60 70 80 90 100
AVQSVRDGLE KRYIASYLAL TVVGMGSWCF HMTLKYEMQL LDELPMIYSC
110 120 130 140 150
CIFVYCMFEC FKIKNSVNYH LLFTLVLFSL IVTTVYLKVK EPIFHQVMYG
160 170 180 190 200
MLVFTLVLRS IYIVTWVYPW LRGLGYTSLG IFLLGFLFWN IDNIFCESLR
210 220 230 240 250
NFRKKVPPII GITTQFHAWW HILTGLGSYL HILFSLYTRT LYLRYRPKVK
260
FLFGIWPVIL FEPLRKH
Length:267
Mass (Da):31,552
Last modified:May 18, 2010 - v3
Checksum:iCFD2A901F12A5918
GO
Isoform 2 (identifier: Q9NUN7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-133: Missing.
     134-145: TVYLKVKEPIFH → MAQSRLIGTSTS

Note: No experimental confirmation available.
Show »
Length:134
Mass (Da):15,895
Checksum:i8333C599D48A5525
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti52V → I in AAK71923 (PubMed:11356846).Curated1
Sequence conflicti52V → I in AAL56013 (Ref. 2) Curated1
Sequence conflicti52V → I in BAG37496 (PubMed:14702039).Curated1
Sequence conflicti52V → I in EAW75010 (Ref. 5) Curated1
Sequence conflicti52V → I in AAH73853 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0391621 – 133Missing in isoform 2. 1 PublicationAdd BLAST133
Alternative sequenceiVSP_039163134 – 145TVYLK…EPIFH → MAQSRLIGTSTS in isoform 2. 1 PublicationAdd BLAST12

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF214454 mRNA. Translation: AAK71923.1.
AF327353 mRNA. Translation: AAL56013.1.
AK002100 mRNA. Translation: BAA92085.1.
AK315000 mRNA. Translation: BAG37496.1.
AP000752 Genomic DNA. No translation available.
AP002498 Genomic DNA. No translation available.
AP003119 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW75010.1.
BC073853 mRNA. Translation: AAH73853.1.
CCDSiCCDS8247.1. [Q9NUN7-1]
RefSeqiNP_060837.3. NM_018367.6. [Q9NUN7-1]
UniGeneiHs.23862.
Hs.720248.

Genome annotation databases

EnsembliENST00000532485; ENSP00000434480; ENSG00000078124. [Q9NUN7-1]
GeneIDi55331.
KEGGihsa:55331.
UCSCiuc009yum.2. human. [Q9NUN7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF214454 mRNA. Translation: AAK71923.1.
AF327353 mRNA. Translation: AAL56013.1.
AK002100 mRNA. Translation: BAA92085.1.
AK315000 mRNA. Translation: BAG37496.1.
AP000752 Genomic DNA. No translation available.
AP002498 Genomic DNA. No translation available.
AP003119 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW75010.1.
BC073853 mRNA. Translation: AAH73853.1.
CCDSiCCDS8247.1. [Q9NUN7-1]
RefSeqiNP_060837.3. NM_018367.6. [Q9NUN7-1]
UniGeneiHs.23862.
Hs.720248.

3D structure databases

ProteinModelPortaliQ9NUN7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120612. 2 interactors.
IntActiQ9NUN7. 1 interactor.
STRINGi9606.ENSP00000434480.

Chemistry databases

SwissLipidsiSLP:000000680.

PTM databases

iPTMnetiQ9NUN7.
PhosphoSitePlusiQ9NUN7.

Polymorphism and mutation databases

BioMutaiACER3.
DMDMi296439452.

Proteomic databases

PaxDbiQ9NUN7.
PeptideAtlasiQ9NUN7.
PRIDEiQ9NUN7.
TopDownProteomicsiQ9NUN7-1. [Q9NUN7-1]

Protocols and materials databases

DNASUi55331.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000532485; ENSP00000434480; ENSG00000078124. [Q9NUN7-1]
GeneIDi55331.
KEGGihsa:55331.
UCSCiuc009yum.2. human. [Q9NUN7-1]

Organism-specific databases

CTDi55331.
DisGeNETi55331.
GeneCardsiACER3.
H-InvDBHIX0021493.
HGNCiHGNC:16066. ACER3.
neXtProtiNX_Q9NUN7.
OpenTargetsiENSG00000078124.
PharmGKBiPA33256.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2329. Eukaryota.
ENOG4111RPN. LUCA.
GeneTreeiENSGT00730000111189.
HOGENOMiHOG000192011.
InParanoidiQ9NUN7.
KOiK04711.
OMAiVYIVLWV.
OrthoDBiEOG091G0DCZ.
PhylomeDBiQ9NUN7.
TreeFamiTF313019.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000078124-MONOMER.
BRENDAi3.5.1.23. 2681.
ReactomeiR-HSA-1660661. Sphingolipid de novo biosynthesis.

Miscellaneous databases

ChiTaRSiACER3. human.
GeneWikiiACER3.
GenomeRNAii55331.
PROiQ9NUN7.

Gene expression databases

BgeeiENSG00000078124.
CleanExiHS_ACER3.
ExpressionAtlasiQ9NUN7. baseline and differential.
GenevisibleiQ9NUN7. HS.

Family and domain databases

InterProiIPR008901. Ceramidase.
[Graphical view]
PfamiPF05875. Ceramidase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACER3_HUMAN
AccessioniPrimary (citable) accession number: Q9NUN7
Secondary accession number(s): B2RC99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: May 18, 2010
Last modified: November 2, 2016
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.