Reviewed, UniProtKB/Swiss-Prot Q9NUN7 (ACER3_HUMAN)

Names and origin

Protein names

Recommended name:
    Alkaline ceramidase 3
      Short name=Alkaline CDase 3
      Short name=AlkCDase 3
    EC=3.5.1.-
Alternative name(s):
    Alkaline phytoceramidase
      Short name=aPHC
    Alkaline dihydroceramidase SB89

Gene names

Name:	ACER3
Synonyms:	APHC, PHCA

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity.
Enzyme regulation	Activated by Ca2+ and inhibited by Zn2+.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein.
Tissue specificity	Ubiquitously expressed. Highest expression in placenta.
Sequence similarities	Belongs to the alkaline ceramidase family.
Sequence caution	The sequence BAA92085.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Domain	Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ceramide metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: InterPro endoplasmic reticulum membrane Ref.1 Inferred from Experiment. Source: Reactome integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phytoceramidase activity Ref.1 Inferred from Experiment. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Alkaline ceramidase 3		PRO_0000212463
Regions
	Transmembrane	29 – 51	23	Potential
	Transmembrane	63 – 84	22	Potential
	Transmembrane	94 – 111	18	Potential
	Transmembrane	118 – 137	20	Potential
	Transmembrane	147 – 169	23	Potential
	Transmembrane	174 – 196	23	Potential
	Transmembrane	216 – 238	23	Potential
Amino acid modifications
	Glycosylation	24	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9NUN7-1 [UniParc]. Last modified August 14, 2001. Version 2. Checksum: 936A8C600D9F38E1	FASTA	267	31,566
	10 20 30 40 50 60 MAPAADREGY WGPTTSTLDW CEENYSVTWY IAEFWNTVSN LIMIIPPMFG AIQSVRDGLE 70 80 90 100 110 120 KRYIASYLAL TVVGMGSWCF HMTLKYEMQL LDELPMIYSC CIFVYCMFEC FKIKNSVNYH 130 140 150 160 170 180 LLFTLVLFSL IVTTVYLKVK EPIFHQVMYG MLVFTLVLRS IYIVTWVYPW LRGLGYTSLG 190 200 210 220 230 240 IFLLGFLFWN IDNIFCESLR NFRKKVPPII GITTQFHAWW HILTGLGSYL HILFSLYTRT 250 260 LYLRYRPKVK FLFGIWPVIL FEPLRKH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a novel human alkaline ceramidase. A mammalian enzyme that hydrolyzes phytoceramide." Mao C., Xu R., Szulc Z.M., Bielawska A., Galadari S.H., Obeid L.M. J. Biol. Chem. 276:26577-26588(2001) [PubMed: 11356846] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Tissue: Kidney.
[2]	Li N., Zhang W., Wan T., Chen T., Cao X. Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta and Thalamus.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF214454 mRNA. Translation: AAK71923.1. AF327353 mRNA. Translation: AAL56013.1. AK002100 mRNA. Translation: BAA92085.1. Frameshift. AK315000 mRNA. Translation: BAG37496.1. CH471076 Genomic DNA. Translation: EAW75010.1. BC073853 mRNA. Translation: AAH73853.1.
IPI	IPI00018961.
RefSeq	NP_060837.3.
UniGene	Hs.23862
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9NUN7.
Proteomic databases
PRIDE	Q9NUN7.
Genome annotation databases
Ensembl	ENST00000278544; ENSP00000278544; ENSG00000078124; Homo sapiens. [Genome view]
GeneID	55331.
KEGG	hsa:55331.
Organism-specific databases
CTD	55331.
GeneCards	GC11P076250.
H-InvDB	HIX0021493.
HGNC	HGNC:16066. ACER3.
PharmGKB	PA33256.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9NUN7.
HOVERGEN	Q9NUN7.
Enzyme and pathway databases
Reactome	REACT_602. Metabolism of lipids and lipoproteins.
Gene expression databases
ArrayExpress	Q9NUN7.
Bgee	Q9NUN7.
CleanEx	HS_ACER3.
Genevestigator	Q9NUN7.
GermOnline	ENSG00000078124. Homo sapiens.
Family and domain databases
InterPro	IPR008901. APHC. [Graphical view]
Pfam	PF05875. aPHC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	59622.

Entry information

Entry name

ACER3_HUMAN

Accession

Primary (citable) accession number: Q9NUN7
Secondary accession number(s): B2RC99

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 14, 2001
Last sequence update:	August 14, 2001
Last modified:	November 24, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.