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Q9NUN7

- ACER3_HUMAN

UniProt

Q9NUN7 - ACER3_HUMAN

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Protein

Alkaline ceramidase 3

Gene

ACER3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity.

Enzyme regulationi

Activated by Ca2+ and inhibited by Zn2+.

GO - Molecular functioni

  1. phytoceramidase activity Source: UniProtKB

GO - Biological processi

  1. ceramide metabolic process Source: InterPro
  2. phytosphingosine biosynthetic process Source: UniProtKB
  3. positive regulation of cell proliferation Source: UniProtKB
  4. small molecule metabolic process Source: Reactome
  5. sphingolipid biosynthetic process Source: Reactome
  6. sphingolipid metabolic process Source: Reactome
  7. sphingosine biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

ReactomeiREACT_115810. Sphingolipid de novo biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline ceramidase 3 (EC:3.5.1.-)
Short name:
AlkCDase 3
Short name:
Alkaline CDase 3
Alternative name(s):
Alkaline dihydroceramidase SB89
Alkaline phytoceramidase
Short name:
aPHC
Gene namesi
Name:ACER3
Synonyms:APHC, PHCA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:16066. ACER3.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: Reactome
  2. integral component of endoplasmic reticulum membrane Source: UniProtKB
  3. integral component of Golgi membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33256.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 267267Alkaline ceramidase 3PRO_0000212463Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi24 – 241N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9NUN7.
PRIDEiQ9NUN7.

PTM databases

PhosphoSiteiQ9NUN7.

Expressioni

Tissue specificityi

Ubiquitously expressed. Highest expression in placenta.

Gene expression databases

BgeeiQ9NUN7.
CleanExiHS_ACER3.
ExpressionAtlasiQ9NUN7. baseline and differential.
GenevestigatoriQ9NUN7.

Interactioni

Protein-protein interaction databases

BioGridi120612. 2 interactions.
IntActiQ9NUN7. 1 interaction.
STRINGi9606.ENSP00000278544.

Structurei

3D structure databases

ProteinModelPortaliQ9NUN7.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei29 – 5123HelicalSequence AnalysisAdd
BLAST
Transmembranei63 – 8422HelicalSequence AnalysisAdd
BLAST
Transmembranei94 – 11118HelicalSequence AnalysisAdd
BLAST
Transmembranei118 – 13720HelicalSequence AnalysisAdd
BLAST
Transmembranei147 – 16923HelicalSequence AnalysisAdd
BLAST
Transmembranei174 – 19623HelicalSequence AnalysisAdd
BLAST
Transmembranei216 – 23823HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline ceramidase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG250726.
GeneTreeiENSGT00730000111189.
HOGENOMiHOG000192011.
InParanoidiQ9NUN7.
KOiK04711.
OMAiMIYSCCV.
PhylomeDBiQ9NUN7.
TreeFamiTF313019.

Family and domain databases

InterProiIPR008901. Ceramidase.
[Graphical view]
PfamiPF05875. Ceramidase. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9NUN7) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPAADREGY WGPTTSTLDW CEENYSVTWY IAEFWNTVSN LIMIIPPMFG
60 70 80 90 100
AVQSVRDGLE KRYIASYLAL TVVGMGSWCF HMTLKYEMQL LDELPMIYSC
110 120 130 140 150
CIFVYCMFEC FKIKNSVNYH LLFTLVLFSL IVTTVYLKVK EPIFHQVMYG
160 170 180 190 200
MLVFTLVLRS IYIVTWVYPW LRGLGYTSLG IFLLGFLFWN IDNIFCESLR
210 220 230 240 250
NFRKKVPPII GITTQFHAWW HILTGLGSYL HILFSLYTRT LYLRYRPKVK
260
FLFGIWPVIL FEPLRKH
Length:267
Mass (Da):31,552
Last modified:May 18, 2010 - v3
Checksum:iCFD2A901F12A5918
GO
Isoform 2 (identifier: Q9NUN7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-133: Missing.
     134-145: TVYLKVKEPIFH → MAQSRLIGTSTS

Note: No experimental confirmation available.

Show »
Length:134
Mass (Da):15,895
Checksum:i8333C599D48A5525
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti52 – 521V → I in AAK71923. (PubMed:11356846)Curated
Sequence conflicti52 – 521V → I in AAL56013. 1 PublicationCurated
Sequence conflicti52 – 521V → I in BAG37496. (PubMed:14702039)Curated
Sequence conflicti52 – 521V → I in EAW75010. 1 PublicationCurated
Sequence conflicti52 – 521V → I in AAH73853. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 133133Missing in isoform 2. 1 PublicationVSP_039162Add
BLAST
Alternative sequencei134 – 14512TVYLK…EPIFH → MAQSRLIGTSTS in isoform 2. 1 PublicationVSP_039163Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF214454 mRNA. Translation: AAK71923.1.
AF327353 mRNA. Translation: AAL56013.1.
AK002100 mRNA. Translation: BAA92085.1.
AK315000 mRNA. Translation: BAG37496.1.
AP000752 Genomic DNA. No translation available.
AP002498 Genomic DNA. No translation available.
AP003119 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW75010.1.
BC073853 mRNA. Translation: AAH73853.1.
CCDSiCCDS8247.1. [Q9NUN7-1]
RefSeqiNP_060837.3. NM_018367.6. [Q9NUN7-1]
UniGeneiHs.23862.
Hs.720248.

Genome annotation databases

EnsembliENST00000532485; ENSP00000434480; ENSG00000078124. [Q9NUN7-1]
GeneIDi55331.
KEGGihsa:55331.
UCSCiuc009yum.1. human. [Q9NUN7-1]

Polymorphism databases

DMDMi296439452.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF214454 mRNA. Translation: AAK71923.1 .
AF327353 mRNA. Translation: AAL56013.1 .
AK002100 mRNA. Translation: BAA92085.1 .
AK315000 mRNA. Translation: BAG37496.1 .
AP000752 Genomic DNA. No translation available.
AP002498 Genomic DNA. No translation available.
AP003119 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW75010.1 .
BC073853 mRNA. Translation: AAH73853.1 .
CCDSi CCDS8247.1. [Q9NUN7-1 ]
RefSeqi NP_060837.3. NM_018367.6. [Q9NUN7-1 ]
UniGenei Hs.23862.
Hs.720248.

3D structure databases

ProteinModelPortali Q9NUN7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 120612. 2 interactions.
IntActi Q9NUN7. 1 interaction.
STRINGi 9606.ENSP00000278544.

PTM databases

PhosphoSitei Q9NUN7.

Polymorphism databases

DMDMi 296439452.

Proteomic databases

PaxDbi Q9NUN7.
PRIDEi Q9NUN7.

Protocols and materials databases

DNASUi 55331.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000532485 ; ENSP00000434480 ; ENSG00000078124 . [Q9NUN7-1 ]
GeneIDi 55331.
KEGGi hsa:55331.
UCSCi uc009yum.1. human. [Q9NUN7-1 ]

Organism-specific databases

CTDi 55331.
GeneCardsi GC11P076571.
H-InvDB HIX0021493.
HGNCi HGNC:16066. ACER3.
neXtProti NX_Q9NUN7.
PharmGKBi PA33256.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG250726.
GeneTreei ENSGT00730000111189.
HOGENOMi HOG000192011.
InParanoidi Q9NUN7.
KOi K04711.
OMAi MIYSCCV.
PhylomeDBi Q9NUN7.
TreeFami TF313019.

Enzyme and pathway databases

Reactomei REACT_115810. Sphingolipid de novo biosynthesis.

Miscellaneous databases

GeneWikii ACER3.
GenomeRNAii 55331.
NextBioi 59622.
PROi Q9NUN7.

Gene expression databases

Bgeei Q9NUN7.
CleanExi HS_ACER3.
ExpressionAtlasi Q9NUN7. baseline and differential.
Genevestigatori Q9NUN7.

Family and domain databases

InterProi IPR008901. Ceramidase.
[Graphical view ]
Pfami PF05875. Ceramidase. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a novel human alkaline ceramidase. A mammalian enzyme that hydrolyzes phytoceramide."
    Mao C., Xu R., Szulc Z.M., Bielawska A., Galadari S.H., Obeid L.M.
    J. Biol. Chem. 276:26577-26588(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION.
    Tissue: Kidney.
  2. Li N., Zhang W., Wan T., Chen T., Cao X.
    Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Placenta and Thalamus.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Eye.

Entry informationi

Entry nameiACER3_HUMAN
AccessioniPrimary (citable) accession number: Q9NUN7
Secondary accession number(s): B2RC99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: May 18, 2010
Last modified: October 29, 2014
This is version 114 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3