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Q9NUN7

- ACER3_HUMAN

UniProt

Q9NUN7 - ACER3_HUMAN

Protein

Alkaline ceramidase 3

Gene

ACER3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 3 (18 May 2010)
      Previous versions | rss
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    Functioni

    Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity.

    Enzyme regulationi

    Activated by Ca2+ and inhibited by Zn2+.

    GO - Molecular functioni

    1. phytoceramidase activity Source: UniProtKB

    GO - Biological processi

    1. ceramide metabolic process Source: InterPro
    2. phytosphingosine biosynthetic process Source: UniProtKB
    3. positive regulation of cell proliferation Source: UniProtKB
    4. small molecule metabolic process Source: Reactome
    5. sphingolipid biosynthetic process Source: Reactome
    6. sphingolipid metabolic process Source: Reactome
    7. sphingosine biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    ReactomeiREACT_115810. Sphingolipid de novo biosynthesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alkaline ceramidase 3 (EC:3.5.1.-)
    Short name:
    AlkCDase 3
    Short name:
    Alkaline CDase 3
    Alternative name(s):
    Alkaline dihydroceramidase SB89
    Alkaline phytoceramidase
    Short name:
    aPHC
    Gene namesi
    Name:ACER3
    Synonyms:APHC, PHCA
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:16066. ACER3.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: Reactome
    2. integral component of endoplasmic reticulum membrane Source: UniProtKB
    3. integral component of Golgi membrane Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33256.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 267267Alkaline ceramidase 3PRO_0000212463Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi24 – 241N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9NUN7.
    PRIDEiQ9NUN7.

    PTM databases

    PhosphoSiteiQ9NUN7.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed. Highest expression in placenta.

    Gene expression databases

    ArrayExpressiQ9NUN7.
    BgeeiQ9NUN7.
    CleanExiHS_ACER3.
    GenevestigatoriQ9NUN7.

    Interactioni

    Protein-protein interaction databases

    BioGridi120612. 2 interactions.
    IntActiQ9NUN7. 1 interaction.
    STRINGi9606.ENSP00000278544.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9NUN7.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei29 – 5123HelicalSequence AnalysisAdd
    BLAST
    Transmembranei63 – 8422HelicalSequence AnalysisAdd
    BLAST
    Transmembranei94 – 11118HelicalSequence AnalysisAdd
    BLAST
    Transmembranei118 – 13720HelicalSequence AnalysisAdd
    BLAST
    Transmembranei147 – 16923HelicalSequence AnalysisAdd
    BLAST
    Transmembranei174 – 19623HelicalSequence AnalysisAdd
    BLAST
    Transmembranei216 – 23823HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alkaline ceramidase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG250726.
    HOGENOMiHOG000192011.
    InParanoidiQ9NUN7.
    KOiK04711.
    OMAiMIYSCCV.
    PhylomeDBiQ9NUN7.
    TreeFamiTF313019.

    Family and domain databases

    InterProiIPR008901. Ceramidase.
    [Graphical view]
    PfamiPF05875. Ceramidase. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9NUN7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAPAADREGY WGPTTSTLDW CEENYSVTWY IAEFWNTVSN LIMIIPPMFG    50
    AVQSVRDGLE KRYIASYLAL TVVGMGSWCF HMTLKYEMQL LDELPMIYSC 100
    CIFVYCMFEC FKIKNSVNYH LLFTLVLFSL IVTTVYLKVK EPIFHQVMYG 150
    MLVFTLVLRS IYIVTWVYPW LRGLGYTSLG IFLLGFLFWN IDNIFCESLR 200
    NFRKKVPPII GITTQFHAWW HILTGLGSYL HILFSLYTRT LYLRYRPKVK 250
    FLFGIWPVIL FEPLRKH 267
    Length:267
    Mass (Da):31,552
    Last modified:May 18, 2010 - v3
    Checksum:iCFD2A901F12A5918
    GO
    Isoform 2 (identifier: Q9NUN7-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-133: Missing.
         134-145: TVYLKVKEPIFH → MAQSRLIGTSTS

    Note: No experimental confirmation available.

    Show »
    Length:134
    Mass (Da):15,895
    Checksum:i8333C599D48A5525
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti52 – 521V → I in AAK71923. (PubMed:11356846)Curated
    Sequence conflicti52 – 521V → I in AAL56013. 1 PublicationCurated
    Sequence conflicti52 – 521V → I in BAG37496. (PubMed:14702039)Curated
    Sequence conflicti52 – 521V → I in EAW75010. 1 PublicationCurated
    Sequence conflicti52 – 521V → I in AAH73853. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 133133Missing in isoform 2. 1 PublicationVSP_039162Add
    BLAST
    Alternative sequencei134 – 14512TVYLK…EPIFH → MAQSRLIGTSTS in isoform 2. 1 PublicationVSP_039163Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF214454 mRNA. Translation: AAK71923.1.
    AF327353 mRNA. Translation: AAL56013.1.
    AK002100 mRNA. Translation: BAA92085.1.
    AK315000 mRNA. Translation: BAG37496.1.
    AP000752 Genomic DNA. No translation available.
    AP002498 Genomic DNA. No translation available.
    AP003119 Genomic DNA. No translation available.
    CH471076 Genomic DNA. Translation: EAW75010.1.
    BC073853 mRNA. Translation: AAH73853.1.
    CCDSiCCDS8247.1. [Q9NUN7-1]
    RefSeqiNP_060837.3. NM_018367.5. [Q9NUN7-1]
    UniGeneiHs.23862.

    Genome annotation databases

    EnsembliENST00000532485; ENSP00000434480; ENSG00000078124. [Q9NUN7-1]
    GeneIDi55331.
    KEGGihsa:55331.
    UCSCiuc009yum.1. human. [Q9NUN7-1]

    Polymorphism databases

    DMDMi296439452.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF214454 mRNA. Translation: AAK71923.1 .
    AF327353 mRNA. Translation: AAL56013.1 .
    AK002100 mRNA. Translation: BAA92085.1 .
    AK315000 mRNA. Translation: BAG37496.1 .
    AP000752 Genomic DNA. No translation available.
    AP002498 Genomic DNA. No translation available.
    AP003119 Genomic DNA. No translation available.
    CH471076 Genomic DNA. Translation: EAW75010.1 .
    BC073853 mRNA. Translation: AAH73853.1 .
    CCDSi CCDS8247.1. [Q9NUN7-1 ]
    RefSeqi NP_060837.3. NM_018367.5. [Q9NUN7-1 ]
    UniGenei Hs.23862.

    3D structure databases

    ProteinModelPortali Q9NUN7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 120612. 2 interactions.
    IntActi Q9NUN7. 1 interaction.
    STRINGi 9606.ENSP00000278544.

    PTM databases

    PhosphoSitei Q9NUN7.

    Polymorphism databases

    DMDMi 296439452.

    Proteomic databases

    PaxDbi Q9NUN7.
    PRIDEi Q9NUN7.

    Protocols and materials databases

    DNASUi 55331.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000532485 ; ENSP00000434480 ; ENSG00000078124 . [Q9NUN7-1 ]
    GeneIDi 55331.
    KEGGi hsa:55331.
    UCSCi uc009yum.1. human. [Q9NUN7-1 ]

    Organism-specific databases

    CTDi 55331.
    GeneCardsi GC11P076571.
    H-InvDB HIX0021493.
    HGNCi HGNC:16066. ACER3.
    neXtProti NX_Q9NUN7.
    PharmGKBi PA33256.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG250726.
    HOGENOMi HOG000192011.
    InParanoidi Q9NUN7.
    KOi K04711.
    OMAi MIYSCCV.
    PhylomeDBi Q9NUN7.
    TreeFami TF313019.

    Enzyme and pathway databases

    Reactomei REACT_115810. Sphingolipid de novo biosynthesis.

    Miscellaneous databases

    GeneWikii ACER3.
    GenomeRNAii 55331.
    NextBioi 59622.
    PROi Q9NUN7.

    Gene expression databases

    ArrayExpressi Q9NUN7.
    Bgeei Q9NUN7.
    CleanExi HS_ACER3.
    Genevestigatori Q9NUN7.

    Family and domain databases

    InterProi IPR008901. Ceramidase.
    [Graphical view ]
    Pfami PF05875. Ceramidase. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of a novel human alkaline ceramidase. A mammalian enzyme that hydrolyzes phytoceramide."
      Mao C., Xu R., Szulc Z.M., Bielawska A., Galadari S.H., Obeid L.M.
      J. Biol. Chem. 276:26577-26588(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION.
      Tissue: Kidney.
    2. Li N., Zhang W., Wan T., Chen T., Cao X.
      Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Placenta and Thalamus.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Eye.

    Entry informationi

    Entry nameiACER3_HUMAN
    AccessioniPrimary (citable) accession number: Q9NUN7
    Secondary accession number(s): B2RC99
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 14, 2001
    Last sequence update: May 18, 2010
    Last modified: October 1, 2014
    This is version 113 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3