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Q9NUN7 (ACER3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alkaline ceramidase 3

Short name=AlkCDase 3
Short name=Alkaline CDase 3
EC=3.5.1.-
Alternative name(s):
Alkaline dihydroceramidase SB89
Alkaline phytoceramidase
Short name=aPHC
Gene names
Name:ACER3
Synonyms:APHC, PHCA
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length267 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity.

Enzyme regulation

Activated by Ca2+ and inhibited by Zn2+.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein.

Tissue specificity

Ubiquitously expressed. Highest expression in placenta.

Sequence similarities

Belongs to the alkaline ceramidase family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9NUN7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9NUN7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-133: Missing.
     134-145: TVYLKVKEPIFH → MAQSRLIGTSTS
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 267267Alkaline ceramidase 3
PRO_0000212463

Regions

Transmembrane29 – 5123Helical; Potential
Transmembrane63 – 8422Helical; Potential
Transmembrane94 – 11118Helical; Potential
Transmembrane118 – 13720Helical; Potential
Transmembrane147 – 16923Helical; Potential
Transmembrane174 – 19623Helical; Potential
Transmembrane216 – 23823Helical; Potential

Amino acid modifications

Glycosylation241N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 133133Missing in isoform 2.
VSP_039162
Alternative sequence134 – 14512TVYLK…EPIFH → MAQSRLIGTSTS in isoform 2.
VSP_039163

Experimental info

Sequence conflict521V → I in AAK71923. Ref.1
Sequence conflict521V → I in AAL56013. Ref.2
Sequence conflict521V → I in BAG37496. Ref.3
Sequence conflict521V → I in EAW75010. Ref.5
Sequence conflict521V → I in AAH73853. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 18, 2010. Version 3.
Checksum: CFD2A901F12A5918

FASTA26731,552
        10         20         30         40         50         60 
MAPAADREGY WGPTTSTLDW CEENYSVTWY IAEFWNTVSN LIMIIPPMFG AVQSVRDGLE 

        70         80         90        100        110        120 
KRYIASYLAL TVVGMGSWCF HMTLKYEMQL LDELPMIYSC CIFVYCMFEC FKIKNSVNYH 

       130        140        150        160        170        180 
LLFTLVLFSL IVTTVYLKVK EPIFHQVMYG MLVFTLVLRS IYIVTWVYPW LRGLGYTSLG 

       190        200        210        220        230        240 
IFLLGFLFWN IDNIFCESLR NFRKKVPPII GITTQFHAWW HILTGLGSYL HILFSLYTRT 

       250        260 
LYLRYRPKVK FLFGIWPVIL FEPLRKH 

« Hide

Isoform 2 [UniParc].

Checksum: 8333C599D48A5525
Show »

FASTA13415,895

References

« Hide 'large scale' references
[1]"Cloning and characterization of a novel human alkaline ceramidase. A mammalian enzyme that hydrolyzes phytoceramide."
Mao C., Xu R., Szulc Z.M., Bielawska A., Galadari S.H., Obeid L.M.
J. Biol. Chem. 276:26577-26588(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION.
Tissue: Kidney.
[2]Li N., Zhang W., Wan T., Chen T., Cao X.
Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Placenta and Thalamus.
[4]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Eye.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF214454 mRNA. Translation: AAK71923.1.
AF327353 mRNA. Translation: AAL56013.1.
AK002100 mRNA. Translation: BAA92085.1.
AK315000 mRNA. Translation: BAG37496.1.
AP000752 Genomic DNA. No translation available.
AP002498 Genomic DNA. No translation available.
AP003119 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW75010.1.
BC073853 mRNA. Translation: AAH73853.1.
RefSeqNP_060837.3. NM_018367.5.
UniGeneHs.23862.

3D structure databases

ProteinModelPortalQ9NUN7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120612. 2 interactions.
IntActQ9NUN7. 1 interaction.
STRING9606.ENSP00000278544.

PTM databases

PhosphoSiteQ9NUN7.

Polymorphism databases

DMDM296439452.

Proteomic databases

PaxDbQ9NUN7.
PRIDEQ9NUN7.

Protocols and materials databases

DNASU55331.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000532485; ENSP00000434480; ENSG00000078124. [Q9NUN7-1]
ENST00000544113; ENSP00000440663; ENSG00000078124. [Q9NUN7-2]
ENST00000571623; ENSP00000459570; ENSG00000262970. [Q9NUN7-1]
GeneID55331.
KEGGhsa:55331.
UCSCuc009yum.1. human. [Q9NUN7-1]

Organism-specific databases

CTD55331.
GeneCardsGC11P076571.
H-InvDBHIX0021493.
HGNCHGNC:16066. ACER3.
neXtProtNX_Q9NUN7.
PharmGKBPA33256.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG250726.
HOGENOMHOG000192011.
InParanoidQ9NUN7.
KOK04711.
OMAMIYSCCV.
PhylomeDBQ9NUN7.
TreeFamTF313019.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.

Gene expression databases

ArrayExpressQ9NUN7.
BgeeQ9NUN7.
CleanExHS_ACER3.
GenevestigatorQ9NUN7.

Family and domain databases

InterProIPR008901. Ceramidase.
[Graphical view]
PfamPF05875. Ceramidase. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiACER3.
GenomeRNAi55331.
NextBio59622.
PROQ9NUN7.

Entry information

Entry nameACER3_HUMAN
AccessionPrimary (citable) accession number: Q9NUN7
Secondary accession number(s): B2RC99
Entry history
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: May 18, 2010
Last modified: April 16, 2014
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM