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Protein

Probable ATP-dependent RNA helicase DDX28

Gene

DDX28

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an essential role in facilitating the proper assembly of the mitochondrial large ribosomal subunit and its helicase activity is essential for this function (PubMed:25683708, PubMed:25683715). May be involved in RNA processing or transport. Has RNA and Mg2+-dependent ATPase activity (PubMed:11350955).3 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi172 – 179ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • RNA binding Source: UniProtKB
  • rRNA binding Source: UniProtKB

GO - Biological processi

  • mitochondrial large ribosomal subunit assembly Source: UniProtKB
  • RNA secondary structure unwinding Source: GO_Central

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding, rRNA-binding
Biological processRibosome biogenesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DDX28 (EC:3.6.4.13)
Alternative name(s):
Mitochondrial DEAD box protein 28
Gene namesi
Name:DDX28
Synonyms:MDDX28
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000182810.6
HGNCiHGNC:17330 DDX28
MIMi607618 gene
neXtProtiNX_Q9NUL7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion, Mitochondrion nucleoid, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi55794
OpenTargetsiENSG00000182810
PharmGKBiPA27214

Polymorphism and mutation databases

BioMutaiDDX28
DMDMi296434476

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000550331 – 540Probable ATP-dependent RNA helicase DDX28Add BLAST540

Proteomic databases

EPDiQ9NUL7
MaxQBiQ9NUL7
PaxDbiQ9NUL7
PeptideAtlasiQ9NUL7
PRIDEiQ9NUL7

PTM databases

iPTMnetiQ9NUL7
PhosphoSitePlusiQ9NUL7

Expressioni

Tissue specificityi

Expressed in all tissues tested, including brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, leukocytes, colon, small intestine, ovary and prostate.1 Publication

Gene expression databases

BgeeiENSG00000182810
CleanExiHS_DDX28
GenevisibleiQ9NUL7 HS

Organism-specific databases

HPAiHPA041911
HPA053585

Interactioni

Subunit structurei

Monomer. Found in a complex with GRSF1, DHX30, FASTKD2 and FASTKD5. Associates with the 16S mitochondrial rRNA (16S mt-rRNA) and with the mitochondrial ribosome large subunit (39S).2 Publications

Protein-protein interaction databases

BioGridi120907, 51 interactors
CORUMiQ9NUL7
IntActiQ9NUL7, 14 interactors
MINTiQ9NUL7
STRINGi9606.ENSP00000332340

Structurei

3D structure databases

ProteinModelPortaliQ9NUL7
SMRiQ9NUL7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini159 – 351Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST193
Domaini377 – 536Helicase C-terminalPROSITE-ProRule annotationAdd BLAST160

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi3 – 18Mitochondrial targeting signalSequence analysisAdd BLAST16
Motifi126 – 156Q motifAdd BLAST31
Motifi180 – 191Nuclear export signalSequence analysisAdd BLAST12
Motifi286 – 289DEAD4
Motifi520 – 523Nuclear localization signalSequence analysis4

Sequence similaritiesi

Belongs to the DEAD box helicase family.Curated

Phylogenomic databases

eggNOGiKOG0330 Eukaryota
ENOG410XQU7 LUCA
GeneTreeiENSGT00730000110914
HOGENOMiHOG000008689
HOVERGENiHBG081424
InParanoidiQ9NUL7
KOiK20096
OMAiLHCIMPH
OrthoDBiEOG091G06DC
PhylomeDBiQ9NUL7
TreeFamiTF324977

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequencei

Sequence statusi: Complete.

Q9NUL7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALTRPVRLF SLVTRLLLAP RRGLTVRSPD EPLPVVRIPV ALQRQLEQRQ
60 70 80 90 100
SRRRNLPRPV LVRPGPLLVS ARRPELNQPA RLTLGRWERA PLASQGWKSR
110 120 130 140 150
RARRDHFSIE RAQQEAPAVR KLSSKGSFAD LGLEPRVLHA LQEAAPEVVQ
160 170 180 190 200
PTTVQSSTIP SLLRGRHVVC AAETGSGKTL SYLLPLLQRL LGQPSLDSLP
210 220 230 240 250
IPAPRGLVLV PSRELAQQVR AVAQPLGRSL GLLVRDLEGG HGMRRIRLQL
260 270 280 290 300
SRQPSADVLV ATPGALWKAL KSRLISLEQL SFLVLDEADT LLDESFLELV
310 320 330 340 350
DYILEKSHIA EGPADLEDPF NPKAQLVLVG ATFPEGVGQL LNKVASPDAV
360 370 380 390 400
TTITSSKLHC IMPHVKQTFL RLKGADKVAE LVHILKHRDR AERTGPSGTV
410 420 430 440 450
LVFCNSSSTV NWLGYILDDH KIQHLRLQGQ MPALMRVGIF QSFQKSSRDI
460 470 480 490 500
LLCTDIASRG LDSTGVELVV NYDFPPTLQD YIHRAGRVGR VGSEVPGTVI
510 520 530 540
SFVTHPWDVS LVQKIELAAR RRRSLPGLAS SVKEPLPQAT
Length:540
Mass (Da):59,581
Last modified:May 18, 2010 - v2
Checksum:i1FAA4477853893B5
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0521634T → A3 PublicationsCorresponds to variant dbSNP:rs237831Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF329821 Genomic DNA Translation: AAG59833.1
AK002144 mRNA Translation: BAA92106.1
AC130462 Genomic DNA No translation available.
BC024273 mRNA Translation: AAH24273.1
CCDSiCCDS10858.1
RefSeqiNP_060850.2, NM_018380.3
UniGeneiHs.458313

Genome annotation databases

EnsembliENST00000332395; ENSP00000332340; ENSG00000182810
GeneIDi55794
KEGGihsa:55794
UCSCiuc002evh.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiDDX28_HUMAN
AccessioniPrimary (citable) accession number: Q9NUL7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: May 18, 2010
Last modified: April 25, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health