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Protein

Repressor of yield of DENV protein

Gene

RYDEN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Exhibits antiviral activity against dengue virus (DENV) and can inhibit the replication of all DENV serotypes. May block the protein translation of DENV RNA via its association with cellular mRNA-binding proteins and viral RNA. Can also limit the replication of hepatitis C virus (HCV), West Nile virus (WNV), Chikungunya virus (CHIKV), herpes simplex virus type 1 (HHV-1) and human adenovirus (PubMed:26735137).1 Publication

GO - Molecular functioni

  • RNA binding Source: UniProtKB

GO - Biological processi

  • defense response to virus Source: UniProtKB
  • negative regulation of viral genome replication Source: UniProtKB
  • response to interferon-gamma Source: UniProtKB
  • response to type III interferon Source: UniProtKB
  • response to type I interferon Source: UniProtKB

Keywordsi

Molecular functionRNA-binding
Biological processAntiviral defense

Names & Taxonomyi

Protein namesi
Recommended name:
Repressor of yield of DENV protein1 Publication
Short name:
RyDEN1 Publication
Gene namesi
Name:RYDEN1 Publication
Synonyms:C19orf66
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:25649. C19orf66.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi121 – 137RRVPQ…RKCRK → AAVPQAAEVSACAACAA: Decreased efficiency in the interaction with PABPC1 and reduced inhibitory activity against DENV replication. 1 PublicationAdd BLAST17

Organism-specific databases

OpenTargetsiENSG00000130813.
PharmGKBiPA162378809.

Polymorphism and mutation databases

BioMutaiC19orf66.
DMDMi172044675.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00003187012 – 291Repressor of yield of DENV proteinAdd BLAST290

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9NUL5.
MaxQBiQ9NUL5.
PaxDbiQ9NUL5.
PeptideAtlasiQ9NUL5.
PRIDEiQ9NUL5.

PTM databases

iPTMnetiQ9NUL5.
PhosphoSitePlusiQ9NUL5.

Expressioni

Inductioni

Up-regulated by interferon (IFN) treatment.1 Publication

Gene expression databases

BgeeiENSG00000130813.
CleanExiHS_C19orf66.
ExpressionAtlasiQ9NUL5. baseline and differential.
GenevisibleiQ9NUL5. HS.

Organism-specific databases

HPAiHPA042001.

Interactioni

Subunit structurei

Interacts with PABPC1 (PubMed:26735137). Found in a complex with PABPC1 and LARP1 (PubMed:26735137).1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi120618. 33 interactors.
IntActiQ9NUL5. 47 interactors.
MINTiMINT-7032143.
STRINGi9606.ENSP00000253110.

Structurei

3D structure databases

ProteinModelPortaliQ9NUL5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni102 – 150Interaction with PABPC11 PublicationAdd BLAST49

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi121 – 137Nuclear localization signal1 PublicationAdd BLAST17
Motifi261 – 269Nuclear export signal1 Publication9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi262 – 291Asp/Glu-rich (acidic)Add BLAST30

Sequence similaritiesi

Belongs to the RyDEN family.Curated

Phylogenomic databases

eggNOGiENOG410IPDV. Eukaryota.
ENOG4110MZD. LUCA.
GeneTreeiENSGT00390000005065.
HOGENOMiHOG000111999.
HOVERGENiHBG061512.
InParanoidiQ9NUL5.
OMAiMWGVAEF.
OrthoDBiEOG090A09PV.
PhylomeDBiQ9NUL5.
TreeFamiTF337433.

Family and domain databases

InterProiView protein in InterPro
IPR026795. RyDEN.
PANTHERiPTHR16135. PTHR16135. 1 hit.
PfamiView protein in Pfam
PF15135. UPF0515. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NUL5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQEGVELEK SVRRLREKFH GKVSSKKAGA LMRKFGSDHT GVGRSIVYGV
60 70 80 90 100
KQKDGQELSN DLDAQDPPED MKQDRDIQAV ATSLLPLTEA NLRMFQRAQD
110 120 130 140 150
DLIPAVDRQF ACSSCDHVWW RRVPQRKEVS RCRKCRKRYE PVPADKMWGL
160 170 180 190 200
AEFHCPKCRH NFRGWAQMGS PSPCYGCGFP VYPTRILPPR WDRDPDRRST
210 220 230 240 250
HTHSCSAADC YNRREPHVPG TSCAHPKSRK QNHLPKVLHP SNPHISSGST
260 270 280 290
VATCLSQGGL LEDLDNLILE DLKEEEEEEE EVEDEEGGPR E
Length:291
Mass (Da):33,110
Last modified:February 26, 2008 - v2
Checksum:iD6513814B96BDAF6
GO
Isoform 2 (identifier: Q9NUL5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-65: MSQEGVELEK...QELSNDLDAQ → MSQHQQACGICRQE

Note: No experimental confirmation available.
Show »
Length:240
Mass (Da):27,524
Checksum:iAF8580711EA1F41B
GO
Isoform 3 (identifier: Q9NUL5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     164-291: Missing.

Show »
Length:163
Mass (Da):18,871
Checksum:iBBB6A14F1DA8ECD7
GO
Isoform 4 (identifier: Q9NUL5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     164-199: Missing.

Show »
Length:255
Mass (Da):29,006
Checksum:i466D43FA57D26D25
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti191W → R in BAA92109 (PubMed:14702039).Curated1
Sequence conflicti249S → P in BAA92109 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0312721 – 65MSQEG…DLDAQ → MSQHQQACGICRQE in isoform 2. 1 PublicationAdd BLAST65
Alternative sequenceiVSP_031273164 – 291Missing in isoform 3. 1 PublicationAdd BLAST128
Alternative sequenceiVSP_031274164 – 199Missing in isoform 4. 1 PublicationAdd BLAST36

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002148 mRNA. Translation: BAA92109.1.
AK096142 mRNA. Translation: BAC04710.1.
AC020931 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84066.1.
CH471106 Genomic DNA. Translation: EAW84068.1.
CH471106 Genomic DNA. Translation: EAW84069.1.
BC010847 mRNA. Translation: AAH10847.1.
BC026180 mRNA. Translation: AAH26180.1.
BC035817 mRNA. Translation: AAH35817.1.
CCDSiCCDS45957.1. [Q9NUL5-1]
CCDS77231.1. [Q9NUL5-4]
RefSeqiNP_001295206.1. NM_001308277.1. [Q9NUL5-4]
NP_060851.2. NM_018381.3. [Q9NUL5-1]
XP_016882423.1. XM_017026934.1. [Q9NUL5-2]
UniGeneiHs.655613.

Genome annotation databases

EnsembliENST00000253110; ENSP00000253110; ENSG00000130813. [Q9NUL5-1]
ENST00000397881; ENSP00000380978; ENSG00000130813. [Q9NUL5-2]
ENST00000591813; ENSP00000467182; ENSG00000130813. [Q9NUL5-4]
GeneIDi55337.
KEGGihsa:55337.
UCSCiuc002mmu.5. human. [Q9NUL5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002148 mRNA. Translation: BAA92109.1.
AK096142 mRNA. Translation: BAC04710.1.
AC020931 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84066.1.
CH471106 Genomic DNA. Translation: EAW84068.1.
CH471106 Genomic DNA. Translation: EAW84069.1.
BC010847 mRNA. Translation: AAH10847.1.
BC026180 mRNA. Translation: AAH26180.1.
BC035817 mRNA. Translation: AAH35817.1.
CCDSiCCDS45957.1. [Q9NUL5-1]
CCDS77231.1. [Q9NUL5-4]
RefSeqiNP_001295206.1. NM_001308277.1. [Q9NUL5-4]
NP_060851.2. NM_018381.3. [Q9NUL5-1]
XP_016882423.1. XM_017026934.1. [Q9NUL5-2]
UniGeneiHs.655613.

3D structure databases

ProteinModelPortaliQ9NUL5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120618. 33 interactors.
IntActiQ9NUL5. 47 interactors.
MINTiMINT-7032143.
STRINGi9606.ENSP00000253110.

PTM databases

iPTMnetiQ9NUL5.
PhosphoSitePlusiQ9NUL5.

Polymorphism and mutation databases

BioMutaiC19orf66.
DMDMi172044675.

Proteomic databases

EPDiQ9NUL5.
MaxQBiQ9NUL5.
PaxDbiQ9NUL5.
PeptideAtlasiQ9NUL5.
PRIDEiQ9NUL5.

Protocols and materials databases

DNASUi55337.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000253110; ENSP00000253110; ENSG00000130813. [Q9NUL5-1]
ENST00000397881; ENSP00000380978; ENSG00000130813. [Q9NUL5-2]
ENST00000591813; ENSP00000467182; ENSG00000130813. [Q9NUL5-4]
GeneIDi55337.
KEGGihsa:55337.
UCSCiuc002mmu.5. human. [Q9NUL5-1]

Organism-specific databases

CTDi55337.
GeneCardsiC19orf66.
HGNCiHGNC:25649. C19orf66.
HPAiHPA042001.
neXtProtiNX_Q9NUL5.
OpenTargetsiENSG00000130813.
PharmGKBiPA162378809.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IPDV. Eukaryota.
ENOG4110MZD. LUCA.
GeneTreeiENSGT00390000005065.
HOGENOMiHOG000111999.
HOVERGENiHBG061512.
InParanoidiQ9NUL5.
OMAiMWGVAEF.
OrthoDBiEOG090A09PV.
PhylomeDBiQ9NUL5.
TreeFamiTF337433.

Miscellaneous databases

ChiTaRSiC19orf66. human.
GenomeRNAii55337.
PROiQ9NUL5.

Gene expression databases

BgeeiENSG00000130813.
CleanExiHS_C19orf66.
ExpressionAtlasiQ9NUL5. baseline and differential.
GenevisibleiQ9NUL5. HS.

Family and domain databases

InterProiView protein in InterPro
IPR026795. RyDEN.
PANTHERiPTHR16135. PTHR16135. 1 hit.
PfamiView protein in Pfam
PF15135. UPF0515. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRYDEN_HUMAN
AccessioniPrimary (citable) accession number: Q9NUL5
Secondary accession number(s): A8MQT9
, Q4G188, Q8IYH6, Q8N8V1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 26, 2008
Last modified: February 15, 2017
This is version 104 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.