Q9NTZ6 (RBM12_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-binding protein 12

Alternative name(s):
RNA-binding motif protein 12
SH3/WW domain anchor protein in the nucleus
Short name=SWAN

Gene names

Name:	RBM12
Synonyms:	KIAA0765
ORF Names:	HRIHFB2091

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	932 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Nucleus Ref.10.
Sequence similarities	Contains 3 RRM (RNA recognition motif) domains.
Sequence caution	The sequence BAA34485.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Repeat
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	nucleus Inferred from direct assay. Source: HPA
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 932

932

RNA-binding protein 12

PRO_0000081770

Regions

Domain

304 – 379

76

RRM 1

Domain

430 – 507

78

RRM 2

Domain

856 – 932

77

RRM 3

Compositional bias

159 – 301

143

Pro-rich

Compositional bias

186 – 194

9

Poly-Pro

Compositional bias

631 – 855

225

Pro-rich

Compositional bias

635 – 854

220

Gly-rich

Amino acid modifications

Modified residue

420

1

Phosphoserine Ref.14

Modified residue

422

1

Phosphoserine Ref.14 Ref.16

Modified residue

424

1

Phosphoserine Ref.13 Ref.14 Ref.16

Natural variations

Natural variant

572

1

N → S.
Corresponds to variant rs17092928 [ dbSNP | Ensembl ].

VAR_052217

Natural variant

921

1

P → L.
Corresponds to variant rs6121012 [ dbSNP | Ensembl ].

VAR_052218

Experimental info

Sequence conflict

601

1

S → T in CAC20441. Ref.1

Secondary structure

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932

Helix Strand Turn

Details...

Helix

301 – 304

4

Beta strand

305 – 309

5

Helix

317 – 323

7

Turn

324 – 326

3

Beta strand

331 – 334

4

Beta strand

344 – 349

6

Helix

353 – 361

9

Turn

362 – 364

3

Beta strand

373 – 376

4

Helix

379 – 386

8

Beta strand

431 – 435

5

Helix

443 – 449

7

Turn

457 – 459

3

Beta strand

461 – 464

4

Beta strand

468 – 481

14

Helix

482 – 488

7

Beta strand

500 – 505

6

Helix

507 – 521

15

Beta strand

545 – 550

6

Helix

557 – 563

7

Turn

564 – 566

3

Beta strand

574 – 576

3

Beta strand

588 – 591

4

Helix

595 – 601

7

Helix

602 – 604

3

Beta strand

606 – 609

4

Beta strand

612 – 619

8

Helix

621 – 629

9

Beta strand

856 – 861

6

Helix

869 – 875

7

Turn

876 – 880

5

Beta strand

887 – 890

4

Beta strand

896 – 906

11

Helix

907 – 917

11

Sequences

	Sequence		Length	Mass (Da)
	Q9NTZ6 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 10623E1EBCC93FC5	FASTA	932	97,395
	10 20 30 40 50 60 MAVVIRLQGL PIVAGTMDIR HFFSGLTIPD GGVHIVGGEL GEAFIVFATD EDARLGMMRT 70 80 90 100 110 120 GGTIKGSKVT LLLSSKTEMQ NMIELSRRRF ETANLDIPPA NASRSGPPPS SGMSSRVNLP 130 140 150 160 170 180 TTVSNFNNPS PSVVTATTSV HESNKNIQTF STASVGTAPP NMGASFGSPT FSSTVPSTAS 190 200 210 220 230 240 PMNTVPPPPI PPIPAMPSLP PMPSIPPIPV PPPVPTLPPV PPVPPIPPVP SVPPMTPLPP 250 260 270 280 290 300 MSGMPPLNPP PVAPLPAGMN GSGAPMNLNN NLNPMFLGPL NPVNPIQMNS QSSVKPLPIN 310 320 330 340 350 360 PDDLYVSVHG MPFSAMENDV RDFFHGLRVD AVHLLKDHVG RNNGNGLVKF LSPQDTFEAL 370 380 390 400 410 420 KRNRMLMIQR YVEVSPATER QWVAAGGHIT FKQNMGPSGQ THPPPQTLPR SKSPSGQKRS 430 440 450 460 470 480 RSRSPHEAGF CVYLKGLPFE AENKHVIDFF KKLDIVEDSI YIAYGPNGKA TGEGFVEFRN 490 500 510 520 530 540 EADYKAALCR HKQYMGNRFI QVHPITKKGM LEKIDMIRKR LQNFSYDQRE MILNPEGDVN 550 560 570 580 590 600 SAKVCAHITN IPFSITKMDV LQFLEGIPVD ENAVHVLVDN NGQGLGQALV QFKNEDDARK 610 620 630 640 650 660 SERLHRKKLN GREAFVHVVT LEDMREIEKN PPAQGKKGLK MPVPGNPAVP GMPNAGLPGV 670 680 690 700 710 720 GLPSAGLPGA GLPSTGLPGS AITSAGLPGA GMPSAGIPSA GGEEHAFLTV GSKEANNGPP 730 740 750 760 770 780 FNFPGNFGGS NAFGPPIPPP GLGGGAFGDA RPGMPSVGNS GLPGLGLDVP GFGGGPNNLS 790 800 810 820 830 840 GPSGFGGGPQ NFGNGPGSLG GPPGFGSGPP GLGSAPGHLG GPPAFGPGPG PGPGPGPIHI 850 860 870 880 890 900 GGPPGFASSS GKPGPTVIKV QNMPFTVSID EILDFFYGYQ VIPGSVCLKY NEKGMPTGEA 910 920 930 MVAFESRDEA TAAVIDLNDR PIGSRKVKLV LG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning, chromosomal assignment, and genomic structure of a human gene encoding a novel member of the RBM family." Stover C.M., Gradl G., Jentsch I., Speicher M.R., Wieser R., Schwaeble W.J. Cytogenet. Cell Genet. 92:225-230(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leukemia.
[2]	"Identification of SWAN as a novel hnRNP-like adaptor protein with multiple domains and broadly expressed in mammalian tissues." Huang C.-H. Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[3]	"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:277-286(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[9]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 270-932. Tissue: Melanoma.
[10]	"Selection system for genes encoding nuclear-targeted proteins." Ueki N., Oda T., Kondo M., Yano K., Noguchi T., Muramatsu M.-A. Nat. Biotechnol. 16:1338-1342(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 557-932, SUBCELLULAR LOCATION. Tissue: Brain.
[11]	"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry." Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C. Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
[12]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[13]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-424, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[14]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-420; SER-422 AND SER-424, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[15]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-422 AND SER-424, MASS SPECTROMETRY.
[17]	"Solution structure of RNA binding domains in RNA-binding protein 12." RIKEN structural genomics initiative (RSGI) Submitted (OCT-2006) to the PDB data bank Cited for: STRUCTURE BY NMR OF 290-638.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ289772 mRNA. Translation: CAC20441.1.
AF345332 mRNA. Translation: AAL83752.1.
AF345335 Genomic DNA. Translation: AAL83755.1.
AF393214 mRNA. Translation: AAM73682.1.
AB018308 mRNA. Translation: BAA34485.2. Different initiation.
AK092239 mRNA. Translation: BAG52504.1.
AL109827 Genomic DNA. Translation: CAB87611.1.
CH471077 Genomic DNA. Translation: EAW76182.1.
CH471077 Genomic DNA. Translation: EAW76184.1.
CH471077 Genomic DNA. Translation: EAW76188.1.
BC012787 mRNA. Translation: AAH12787.1.
BC013981 mRNA. Translation: AAH13981.1.
AL834472 mRNA. Translation: CAD39131.1.
AB015336 mRNA. Translation: BAA34794.1.

IPI

IPI00550308.

RefSeq

NP_001185767.1. NM_001198838.1.
NP_001185769.1. NM_001198840.1.
NP_006038.2. NM_006047.5.
NP_690051.1. NM_152838.3.

UniGene

Hs.700188.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WEL	NMR	-	A	412-522	[»]
2CPY	NMR	-	A	536-636	[»]
2DNN	NMR	-	A	295-390	[»]
2EK1	X-ray	2.00	A/B/C/D/E/F/G/H	848-929	[»]
2EK6	X-ray	2.38	A/B/C/D	848-929	[»]

ProteinModelPortal

Q9NTZ6.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9NTZ6. 5 interactions.

MINT

MINT-1366084.

STRING

9606.ENSP00000352668.

PTM databases

PhosphoSite

Q9NTZ6.

Polymorphism databases

DMDM

30173387.

Proteomic databases

PaxDb

Q9NTZ6.

PeptideAtlas

Q9NTZ6.

PRIDE

Q9NTZ6.

Protocols and materials databases

DNASU

10137.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000359646; ENSP00000352668; ENSG00000244462.
ENST00000374104; ENSP00000363217; ENSG00000244462.
ENST00000374114; ENSP00000363228; ENSG00000244462.

GeneID

10137.

KEGG

hsa:10137.

UCSC

uc002xdq.3. human.

Organism-specific databases

CTD

10137.

GeneCards

GC20M034236.

HGNC

HGNC:9898. RBM12.

HPA

HPA043258.
HPA043621.

MIM

607179. gene.

neXtProt

NX_Q9NTZ6.

PharmGKB

PA34261.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG299235.

HOVERGEN

HBG057691.

InParanoid

Q9NTZ6.

OMA

PVFLGPL.

OrthoDB

EOG44TP7C.

PhylomeDB

Q9NTZ6.

Gene expression databases

ArrayExpress

Q9NTZ6.

Bgee

Q9NTZ6.

CleanEx

HS_RBM12.

Genevestigator

Q9NTZ6.

GermOnline

ENSG00000125976. Homo sapiens.

Family and domain databases

Gene3D

3.30.70.330. 5 hits.

InterPro

IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]

SMART

SM00360. RRM. 3 hits.
[Graphical view]

PROSITE

PS50102. RRM. 3 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9NTZ6.

GenomeRNAi

10137.

NextBio

38349.

SOURCE

Search...

Entry information

Entry name

RBM12_HUMAN

Accession

Primary (citable) accession number: Q9NTZ6
Secondary accession number(s): B3KRU2 , E1P5R6, O94865, Q8N3B1, Q9H196

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 119 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.