Q9NTN9 (SEM4G_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Semaphorin-4G

Gene names

Name:	SEMA4G
Synonyms:	KIAA1619

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	838 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cell surface receptor for PLXNB2. May play a role in axon guidance By similarity.
Subunit structure	Interacts with PLXNB2 By similarity.
Subcellular location	Cell membrane; Single-pass type I membrane protein.
Sequence similarities	Belongs to the semaphorin family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 PSI domain. Contains 1 Sema domain.
Sequence caution	The sequence BAB13445.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Immunoglobulin domain Signal Transmembrane Transmembrane helix
Molecular function	Developmental protein
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW nervous system development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NTN9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NTN9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     543-543: R → RSQGSR

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9NTN9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     543-543: R → RSQGSR
     565-838: PPPPLKTRSV...LVEQLDESSV → RALQVHMGSM...PHSPWSFSRV

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 838	821	Semaphorin-4G		PRO_0000032333
Regions
	Topological domain	18 – 675	658	Extracellular Potential
	Transmembrane	676 – 696	21	Helical; Potential
	Topological domain	697 – 838	142	Cytoplasmic Potential
	Domain	35 – 505	471	Sema
	Domain	507 – 558	52	PSI
	Domain	567 – 649	83	Ig-like C2-type
	Compositional bias	565 – 568	4	Poly-Pro
	Compositional bias	763 – 774	12	Poly-Pro
Amino acid modifications
	Modified residue	795	1	Phosphoserine By similarity
	Glycosylation	55	1	N-linked (GlcNAc...) Potential
	Glycosylation	111	1	N-linked (GlcNAc...) Potential
	Glycosylation	126	1	N-linked (GlcNAc...) Potential
	Glycosylation	388	1	N-linked (GlcNAc...) Potential
	Glycosylation	542	1	N-linked (GlcNAc...) Potential
	Glycosylation	598	1	N-linked (GlcNAc...) Potential
	Disulfide bond	104 ↔ 115		By similarity
	Disulfide bond	133 ↔ 142		By similarity
	Disulfide bond	270 ↔ 377		By similarity
	Disulfide bond	294 ↔ 337		By similarity
	Disulfide bond	508 ↔ 525		By similarity
	Disulfide bond	517 ↔ 534		By similarity
	Disulfide bond	584 ↔ 632		By similarity
Natural variations
	Alternative sequence	543	1	R → RSQGSR in isoform 2 and isoform 3.		VSP_035067
	Alternative sequence	565 – 838	274	PPPPL…DESSV → RALQVHMGSMSPPSAWPCVL DGPETRQDLCQPPKPCVHSH AHMEECLSAGLQCPHPHLLL VHSCFIPASGLGVPSQLPHP IWSSSPAPCGDLFVKSLGTG QPGEVRLHHSPPLPSCVALV NQPPHSPWSFSRV in isoform 3.		VSP_043883

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 9B281AEE8681F245	FASTA	838	91,497
	10 20 30 40 50 60 MWGRLWPLLL SILTATAVPG PSLRRPSREL DATPRMTIPY EELSGTRHFK GQAQNYSTLL 70 80 90 100 110 120 LEEASARLLV GARGALFSLS ANDIGDGAHK EIHWEASPEM QSKCHQKGKN NQTECFNHVR 130 140 150 160 170 180 FLQRLNSTHL YACGTHAFQP LCAAIDAEAF TLPTSFEEGK EKCPYDPARG FTGLIIDGGL 190 200 210 220 230 240 YTATRYEFRS IPDIRRSRHP HSLRTEETPM HWLNDAEFVF SVLVRESKAS AVGDDDKVYY 250 260 270 280 290 300 FFTERATEEG SGSFTQSRSS HRVARVARVC KGDLGGKKIL QKKWTSFLKA RLICHIPLYE 310 320 330 340 350 360 TLRGVCSLDA ETSSRTHFYA AFTLSTQWKT LEASAICRYD LAEIQAVFAG PYMEYQDGSR 370 380 390 400 410 420 RWGRYEGGVP EPRPGSCITD SLRSQGYNSS QDLPSLVLDF VKLHPLMARP VVPTRGRPLL 430 440 450 460 470 480 LKRNIRYTHL TGTPVTTPAG PTYDLLFLGT ADGWIHKAVV LGSGMHIIEE TQVFRESQSV 490 500 510 520 530 540 ENLVISLLQH SLYVGAPSGV IQLPLSSCSR YRSCYDCILA RDPYCGWDPG THACAAATTI 550 560 570 580 590 600 ANRTALIQDI ERGNRGCESS RDTGPPPPLK TRSVLRGDDV LLPCDQPSNL ARALWLLNGS 610 620 630 640 650 660 MGLSDGQGGY RVGVDGLLVT DAQPEHSGNY GCYAEENGLR TLLASYSLTV RPATPAPAPK 670 680 690 700 710 720 APATPGAQLA PDVRLLYVLA IAALGGLCLI LASSLLYVAC LREGRRGRRR KYSLGRASRA 730 740 750 760 770 780 GGSAVQLQTV SGQCPGEEDE GDDEGAGGLE GSCLQIIPGE GAPAPPPPPP PPPPAELTNG 790 800 810 820 830 LVALPSRLRR MNGNSYVLLR QSNNGVPAGP CSFAEELSRI LEKRKHTQLV EQLDESSV
	Isoform 2 [UniParc]. Checksum: 6AEE7436BCEC60CD	FASTA	843	92,012
	10 20 30 40 50 60 MWGRLWPLLL SILTATAVPG PSLRRPSREL DATPRMTIPY EELSGTRHFK GQAQNYSTLL 70 80 90 100 110 120 LEEASARLLV GARGALFSLS ANDIGDGAHK EIHWEASPEM QSKCHQKGKN NQTECFNHVR 130 140 150 160 170 180 FLQRLNSTHL YACGTHAFQP LCAAIDAEAF TLPTSFEEGK EKCPYDPARG FTGLIIDGGL 190 200 210 220 230 240 YTATRYEFRS IPDIRRSRHP HSLRTEETPM HWLNDAEFVF SVLVRESKAS AVGDDDKVYY 250 260 270 280 290 300 FFTERATEEG SGSFTQSRSS HRVARVARVC KGDLGGKKIL QKKWTSFLKA RLICHIPLYE 310 320 330 340 350 360 TLRGVCSLDA ETSSRTHFYA AFTLSTQWKT LEASAICRYD LAEIQAVFAG PYMEYQDGSR 370 380 390 400 410 420 RWGRYEGGVP EPRPGSCITD SLRSQGYNSS QDLPSLVLDF VKLHPLMARP VVPTRGRPLL 430 440 450 460 470 480 LKRNIRYTHL TGTPVTTPAG PTYDLLFLGT ADGWIHKAVV LGSGMHIIEE TQVFRESQSV 490 500 510 520 530 540 ENLVISLLQH SLYVGAPSGV IQLPLSSCSR YRSCYDCILA RDPYCGWDPG THACAAATTI 550 560 570 580 590 600 ANRSQGSRTA LIQDIERGNR GCESSRDTGP PPPLKTRSVL RGDDVLLPCD QPSNLARALW 610 620 630 640 650 660 LLNGSMGLSD GQGGYRVGVD GLLVTDAQPE HSGNYGCYAE ENGLRTLLAS YSLTVRPATP 670 680 690 700 710 720 APAPKAPATP GAQLAPDVRL LYVLAIAALG GLCLILASSL LYVACLREGR RGRRRKYSLG 730 740 750 760 770 780 RASRAGGSAV QLQTVSGQCP GEEDEGDDEG AGGLEGSCLQ IIPGEGAPAP PPPPPPPPPA 790 800 810 820 830 840 ELTNGLVALP SRLRRMNGNS YVLLRQSNNG VPAGPCSFAE ELSRILEKRK HTQLVEQLDE SSV
	Isoform 3 [UniParc]. Checksum: C1D05183AF6793CA	FASTA	702	77,577
	10 20 30 40 50 60 MWGRLWPLLL SILTATAVPG PSLRRPSREL DATPRMTIPY EELSGTRHFK GQAQNYSTLL 70 80 90 100 110 120 LEEASARLLV GARGALFSLS ANDIGDGAHK EIHWEASPEM QSKCHQKGKN NQTECFNHVR 130 140 150 160 170 180 FLQRLNSTHL YACGTHAFQP LCAAIDAEAF TLPTSFEEGK EKCPYDPARG FTGLIIDGGL 190 200 210 220 230 240 YTATRYEFRS IPDIRRSRHP HSLRTEETPM HWLNDAEFVF SVLVRESKAS AVGDDDKVYY 250 260 270 280 290 300 FFTERATEEG SGSFTQSRSS HRVARVARVC KGDLGGKKIL QKKWTSFLKA RLICHIPLYE 310 320 330 340 350 360 TLRGVCSLDA ETSSRTHFYA AFTLSTQWKT LEASAICRYD LAEIQAVFAG PYMEYQDGSR 370 380 390 400 410 420 RWGRYEGGVP EPRPGSCITD SLRSQGYNSS QDLPSLVLDF VKLHPLMARP VVPTRGRPLL 430 440 450 460 470 480 LKRNIRYTHL TGTPVTTPAG PTYDLLFLGT ADGWIHKAVV LGSGMHIIEE TQVFRESQSV 490 500 510 520 530 540 ENLVISLLQH SLYVGAPSGV IQLPLSSCSR YRSCYDCILA RDPYCGWDPG THACAAATTI 550 560 570 580 590 600 ANRSQGSRTA LIQDIERGNR GCESSRDTGR ALQVHMGSMS PPSAWPCVLD GPETRQDLCQ 610 620 630 640 650 660 PPKPCVHSHA HMEECLSAGL QCPHPHLLLV HSCFIPASGL GVPSQLPHPI WSSSPAPCGD 670 680 690 700 LFVKSLGTGQ PGEVRLHHSP PLPSCVALVN QPPHSPWSFS RV

References

[1]	"Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Nakayama M., Hirosawa M., Ohara O. DNA Res. 7:273-281(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB046839 mRNA. Translation: BAB13445.1. Different initiation. AL133215 Genomic DNA. No translation available. BC051030 mRNA. Translation: AAH51030.1. BC128579 mRNA. Translation: AAI28580.1.
IPI	IPI00016629. IPI00018264. IPI00478294.
RefSeq	NP_001190173.1. NM_001203244.1. NP_060363.2. NM_017893.3.
UniGene	Hs.591923. Hs.735152.
3D structure databases
ProteinModelPortal	Q9NTN9.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NTN9. 1 interaction.
MINT	MINT-1475595.
STRING	9606.ENSP00000210633.
PTM databases
PhosphoSite	Q9NTN9.
Polymorphism databases
DMDM	13633937.
Proteomic databases
PaxDb	Q9NTN9.
PRIDE	Q9NTN9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000210633; ENSP00000210633; ENSG00000095539. ENST00000370250; ENSP00000359270; ENSG00000095539. ENST00000517724; ENSP00000430175; ENSG00000095539. ENST00000521006; ENSP00000430881; ENSG00000095539.
GeneID	57715.
KEGG	hsa:57715.
UCSC	uc001krw.2. human. uc010qpt.1. human.
Organism-specific databases
CTD	57715.
GeneCards	GC10P102719.
HGNC	HGNC:10735. SEMA4G.
HPA	HPA038362.
neXtProt	NX_Q9NTN9.
PharmGKB	PA35657.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG309161.
HOGENOM	HOG000116087.
HOVERGEN	HBG061165.
KO	K06521.
OMA	YEFRSSP.
OrthoDB	EOG483D3Z.
Gene expression databases
ArrayExpress	Q9NTN9.
Bgee	Q9NTN9.
CleanEx	HS_SEMA4G.
Genevestigator	Q9NTN9.
GermOnline	ENSG00000095539. Homo sapiens.
Family and domain databases
Gene3D	2.130.10.10. 1 hit. 2.60.40.10. 1 hit.
InterPro	IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR003659. Plexin-like. IPR016201. Plexin-like_fold. IPR002165. Plexin_repeat. IPR027231. Semaphorin. IPR001627. Semaphorin/CD100_Ag. IPR015943. WD40/YVTN_repeat-like_dom. [Graphical view]
PANTHER	PTHR11036. PTHR11036. 1 hit.
Pfam	PF01437. PSI. 1 hit. PF01403. Sema. 1 hit. [Graphical view]
SMART	SM00409. IG. 1 hit. SM00423. PSI. 1 hit. SM00630. Sema. 1 hit. [Graphical view]
SUPFAM	SSF103575. Plexin-like_fold. 1 hit. SSF101912. Sema. 1 hit.
PROSITE	PS50835. IG_LIKE. 1 hit. PS51004. SEMA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SEMA4G. human.
GenomeRNAi	57715.
NextBio	64634.

Entry information

Entry name

SEM4G_HUMAN

Accession

Primary (citable) accession number: Q9NTN9
Secondary accession number(s): A1A5C6 , A6NJY8, Q58EY1, Q9HCF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.