Q9NTM9 (CUTC_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Copper homeostasis protein cutC homolog

Gene names

Name:	CUTC
ORF Names:	CGI-32

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in copper homeostasis. Can bind one Cu1+ per subunit. Ref.6 Ref.8
Subunit structure	Homotetramer. Ref.8
Subcellular location	Cytoplasm. Nucleus. Note: The overexpressed protein is detected in the cytoplasm, and depending on the cell line, also in the nucleus. Ref.6
Tissue specificity	Ubiquitous. Ref.6
Sequence similarities	Belongs to the CutC family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
Ligand	Copper Metal-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	copper ion homeostasis Non-traceable author statement Ref.6. Source: UniProtKB copper ion transport Non-traceable author statement Ref.6. Source: UniProtKB protein tetramerization Inferred from physical interaction Ref.8. Source: UniProtKB
Cellular_component	aggresome Inferred from direct assay. Source: HPA cytoplasm Inferred from direct assay Ref.6. Source: UniProtKB nucleolus Inferred from direct assay. Source: HPA
Molecular_function	copper ion binding Inferred from direct assay Ref.8. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 273

273

Copper homeostasis protein cutC homolog

PRO_0000215088

Natural variations

Natural variant

77

1

P → L in a breast cancer sample; somatic mutation. Ref.9

VAR_036363

Experimental info

Mutagenesis

31

1

C → A: Reduces copper binding. Reduces copper binding by 75%; when associated with A-52. Ref.8

Mutagenesis

52

1

C → A: Reduces copper binding. Reduces copper binding by 75%; when associated with A-31. Ref.8

Sequence conflict

108

1

L → P in AAD27741. Ref.1

Sequence conflict

186

1

K → N in AAD27741. Ref.1

Secondary structure

1

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273

Helix Strand Turn

Details...

Beta strand

26 – 34

9

Helix

35 – 44

10

Beta strand

47 – 51

5

Helix

55 – 57

3

Helix

64 – 71

8

Beta strand

78 – 81

4

Beta strand

84 – 87

4

Helix

93 – 108

16

Beta strand

112 – 116

5

Helix

127 – 137

11

Beta strand

142 – 144

3

Helix

146 – 150

5

Helix

154 – 164

11

Beta strand

167 – 171

5

Beta strand

175 – 177

3

Turn

178 – 181

4

Helix

182 – 192

11

Beta strand

195 – 200

6

Turn

206 – 208

3

Helix

209 – 216

8

Beta strand

219 – 223

5

Beta strand

226 – 229

4

Turn

244 – 246

3

Beta strand

253 – 256

4

Helix

258 – 271

14

Sequences

	Sequence		Length	Mass (Da)
	Q9NTM9 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 63C007AAB05C5492	FASTA	273	29,341
	10 20 30 40 50 60 MKRQGASSER KRARIPSGKA GAANGFLMEV CVDSVESAVN AERGGADRIE LCSGLSEGGT 70 80 90 100 110 120 TPSMGVLQVV KQSVQIPVFV MIRPRGGDFL YSDREIEVMK ADIRLAKLYG ADGLVFGALT 130 140 150 160 170 180 EDGHIDKELC MSLMAICRPL PVTFHRAFDM VHDPMAALET LLTLGFERVL TSGCDSSALE 190 200 210 220 230 240 GLPLIKRLIE QAKGRIVVMP GGGITDRNLQ RILEGSGATE FHCSARSTRD SGMKFRNSSV 250 260 270 AMGASLSCSE YSLKVTDVTK VRTLNAIAKN ILV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics." Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C. Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skeletal muscle.
[3]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Skin.
[6]	"Identification and characterization of a novel Cut family cDNA that encodes human copper transporter protein CutC." Li J., Ji C., Chen J., Yang Z., Wang Y., Fei X., Zheng M., Gu X., Wen G., Xie Y., Mao Y. Biochem. Biophys. Res. Commun. 337:179-183(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"Crystal structure of human copper homeostasis protein CutC reveals a potential copper-binding site." Li Y., Du J., Zhang P., Ding J. J. Struct. Biol. 169:399-405(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), COPPER-BINDING, FUNCTION, SUBUNIT, MUTAGENESIS OF CYS-31 AND CYS-52.
[9]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] LEU-77.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF132966 mRNA. Translation: AAD27741.1.
AK314687 mRNA. Translation: BAG37239.1.
AL133353 Genomic DNA. Translation: CAB88199.1.
CH471066 Genomic DNA. Translation: EAW49854.1.
BC021105 mRNA. Translation: AAH21105.1.
BC028948 mRNA. Translation: AAH28948.1.

IPI

IPI00300408.

RefSeq

NP_057044.2. NM_015960.2.

UniGene

Hs.16606.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3IWP	X-ray	2.50	A/B/C/D/E/F/G/H/I/J/K/L	1-273	[»]

ProteinModelPortal

Q9NTM9.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9NTM9. 4 interactions.

MINT

MINT-1387697.

STRING

9606.ENSP00000359507.

PTM databases

PhosphoSite

Q9NTM9.

Polymorphism databases

DMDM

54035909.

Proteomic databases

PaxDb

Q9NTM9.

PeptideAtlas

Q9NTM9.

PRIDE

Q9NTM9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000370476; ENSP00000359507; ENSG00000119929.

GeneID

51076.

KEGG

hsa:51076.

UCSC

uc001kqd.4. human.

Organism-specific databases

CTD

51076.

GeneCards

GC10P101481.

HGNC

HGNC:24271. CUTC.

HPA

HPA038619.

MIM

610101. gene.

neXtProt

NX_Q9NTM9.

PharmGKB

PA134901980.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG3142.

HOGENOM

HOG000100908.

HOVERGEN

HBG051267.

InParanoid

Q9NTM9.

KO

K06201.

OMA

PVYAMIR.

OrthoDB

EOG4VT5Z1.

PhylomeDB

Q9NTM9.

Gene expression databases

ArrayExpress

Q9NTM9.

Bgee

Q9NTM9.

CleanEx

HS_CUTC.

Genevestigator

Q9NTM9.

GermOnline

ENSG00000119929. Homo sapiens.

Family and domain databases

Gene3D

3.20.20.380. 1 hit.

InterPro

IPR005627. Cu_homeostasis_CutC.
IPR023648. Cu_homeostasis_CutC_dom.
[Graphical view]

PANTHER

PTHR12598. PTHR12598. 1 hit.

Pfam

PF03932. CutC. 1 hit.
[Graphical view]

SUPFAM

SSF110395. CutC. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q9NTM9.

GenomeRNAi

51076.

NextBio

53707.

SOURCE

Search...

Entry information

Entry name

CUTC_HUMAN

Accession

Primary (citable) accession number: Q9NTM9
Secondary accession number(s): Q5TCZ8, Q9Y321

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.