##gff-version 3
Q9NT62	UniProtKB	Chain	1	314	.	.	.	ID=PRO_0000213569;Note=Ubiquitin-like-conjugating enzyme ATG3	
Q9NT62	UniProtKB	Region	140	170	.	.	.	Note=Interaction with ATG12;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:24191030;Dbxref=PMID:24191030	
Q9NT62	UniProtKB	Region	143	163	.	.	.	Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
Q9NT62	UniProtKB	Motif	4	19	.	.	.	Note=Membrane-curvature-sensing motif;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q9CPX6	
Q9NT62	UniProtKB	Motif	101	103	.	.	.	Note=Increases ATG3 translation efficiency by the ribomome assisted of EIF5A;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q9CPX6	
Q9NT62	UniProtKB	Motif	104	110	.	.	.	Note=LIR motif;Ontology_term=ECO:0000269,ECO:0007744;evidence=ECO:0000269|PubMed:37252361,ECO:0007744|PDB:8AFI;Dbxref=PMID:37252361	
Q9NT62	UniProtKB	Motif	166	169	.	.	.	Note=Caspase cleavage motif LETD;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22644571;Dbxref=PMID:22644571	
Q9NT62	UniProtKB	Active site	264	264	.	.	.	Note=Glycyl thioester intermediate;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:11825910;Dbxref=PMID:11825910	
Q9NT62	UniProtKB	Site	169	170	.	.	.	Note=Cleavage%3B by CASP8;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22644571;Dbxref=PMID:22644571	
Q9NT62	UniProtKB	Modified residue	1	1	.	.	.	Note=N-acetylmethionine;Ontology_term=ECO:0007744,ECO:0007744;evidence=ECO:0007744|PubMed:19413330,ECO:0007744|PubMed:22223895;Dbxref=PMID:19413330,PMID:22223895	
Q9NT62	UniProtKB	Cross-link	243	243	.	.	.	Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ATG12);Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q9CPX6	
Q9NT62	UniProtKB	Alternative sequence	290	314	.	.	.	ID=VSP_013037;Note=In isoform 2. LLIFLKFVQAVIPTIEYDYTRHFTM->PSLYVRLVAKWLLTIFFLRNLV;Ontology_term=ECO:0000303,ECO:0000303;evidence=ECO:0000303|PubMed:14702039,ECO:0000303|PubMed:15489334;Dbxref=PMID:14702039,PMID:15489334	
Q9NT62	UniProtKB	Mutagenesis	8	8	.	.	.	Note=Significantly reduces ATG8-family conjugating enzyme activity%3B when associated with K-15. Decreases protein membrane interactions%3B when associated with K-15. Does not affect formation of the covalent thioester intermediate with MAP1LC3B%3B when associated with K-15. V->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33446636;Dbxref=PMID:33446636	
Q9NT62	UniProtKB	Mutagenesis	9	9	.	.	.	Note=Slightly decreases interaction with lipid monolayers%3B when associated with D-11. Slightly decreases insertion into lipid monolayers%3B when associated with D-11. Decreases affinity for monolayers containing cardiolipin (CL)%3B when associated with D-11. Cancels selectivity for anionic phospholipids%3B when associated with D-11. Impairs binding to lipid bilayers%3B when associated with D-11. Impairs insertion into lipid bilayers%3B when associated with D-11. Does not induce vesicle tethering%3B when associated with D-11. K->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:29142222;Dbxref=PMID:29142222	
Q9NT62	UniProtKB	Mutagenesis	11	11	.	.	.	Note=Slightly decreases interaction with lipid monolayers%3B when associated with D-9. Slightly decreases insertion into lipid monolayers%3B when associated with D-9. Decreases affinity for monolayers containing cardiolipin (CL)%3B when associated with D-9. Cancels selectivity for anionic phospholipids%3B when associated with D-11. Impairs binding to lipid bilayers%3B when associated with D-11. Impairs insertion into lipid bilayers%3B when associated with D-11. Does not induce vesicle tethering%3B when associated with D-11. K->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:29142222;Dbxref=PMID:29142222	
Q9NT62	UniProtKB	Mutagenesis	15	15	.	.	.	Note=Significantly reduces ATG8-family conjugating enzyme activity%3B when associated with D-8. Decreases protein membrane interactions%3B when associated with D-8. Does not affect formation of the covalent thioester intermediate with MAP1LC3B%3B when associated with D-8. V->K;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33446636;Dbxref=PMID:33446636	
Q9NT62	UniProtKB	Mutagenesis	21	21	.	.	.	Note=Impairs ATG8-family conjugating enzyme activity. Does not affect protein membrane interactions. Does not affect formation of the covalent thioester intermediate with MAP1LC3B. Partially rescues MAP1LC3B lipidation in cell lacking ATG3. P->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33446636;Dbxref=PMID:33446636	
Q9NT62	UniProtKB	Mutagenesis	23	23	.	.	.	Note=Severely reduces ATG8-family conjugating enzyme activity. Reduces of about 40%25 protein membrane interactions. Does not affect formation of the covalent thioester intermediate with MAP1LC3B. Completely fails to induce membrane-bound MAP1LC3B (MAP1LC3B-II) and autophagic flux when expressed in cell lacking ATG3. L->T;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33446636;Dbxref=PMID:33446636	
Q9NT62	UniProtKB	Mutagenesis	25	25	.	.	.	Note=Increases of about 30%25 more ATG8-family conjugating enzyme activity. Does not affect protein membrane interactions. Does not affect formation of the covalent thioester intermediate with MAP1LC3B. E->K;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33446636;Dbxref=PMID:33446636	
Q9NT62	UniProtKB	Mutagenesis	95	95	.	.	.	Note=Severely diminish GABARAP:ATG3 conjugation. E->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:37252361;Dbxref=PMID:37252361	
Q9NT62	UniProtKB	Mutagenesis	97	97	.	.	.	Note=Severely diminish GABARAP:ATG3 conjugation. I->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:37252361;Dbxref=PMID:37252361	
Q9NT62	UniProtKB	Mutagenesis	107	107	.	.	.	Note=Almost completely abrogates GABARAP:ATG3 conjugation. W->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:37252361;Dbxref=PMID:37252361	
Q9NT62	UniProtKB	Mutagenesis	108	108	.	.	.	Note=Significantly reduces GABARAP:ATG3 conjugation. V->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:37252361;Dbxref=PMID:37252361	
Q9NT62	UniProtKB	Mutagenesis	144	151	.	.	.	Note=Strongly decreases affinity for the complex ATG12:ATG5:ATG16L1. Severely decreases phosphatidylethanolamine (PE)-conjugated ATG8-like proteins formation. EEEEDEDE->AAAAAAAA;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:24191030;Dbxref=PMID:24191030	
Q9NT62	UniProtKB	Mutagenesis	156	156	.	.	.	Note=Strongly decreases affinity for the complex ATG12:ATG5:ATG16L1. Impairs interaction with the complex ATG12:ATG5:ATG16L1%3B when associated with A-157. Impairs phosphatidylethanolamine (PE)-conjugated ATG8-like protein formation. Loss of phosphatidylethanolamine (PE)-conjugated ATG8-like proteins formation%3B when associated with A-157. Impairs interaction with ATG12:ATG5%3B when associated with A-157. Does not affect interaction with ATG7%3B when associated with A-157. Does not affect the formation of the ATG8-like protein:ATG3 intermediate complex%3B when associated with A-157. Affects modestly the ATG3-ATG7 interaction%3B when associated with A-157. Reduces the thioester-linkage formation with GABARAP%3B when associated with A-157. D->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:24191030,ECO:0000269|PubMed:26043688;Dbxref=PMID:24191030,PMID:26043688	
Q9NT62	UniProtKB	Mutagenesis	157	157	.	.	.	Note=Strongly decreases affinity for the complex ATG12:ATG5:ATG16L1. Impairs interaction with the complex ATG12:ATG5:ATG16L1%3B when associated with A-156. Impairs phosphatidylethanolamine (PE)-conjugated ATG8-like proteins formation. Loss of phosphatidylethanolamine (PE)-conjugated ATG8-like proteins formation%3B when associated with A-156. Impairs interaction with ATG12:ATG5%3B when associated with A-156. Does not affect interaction with ATG7%3B when associated with A-156. Does not affect the formation of the ATG8-like protein:ATG3 intermediate complex%3B when associated with A-156. Affects modestly the ATG3-ATG7 interaction%3B when associated with A-156. Reduces the thioester-linkage formation with GABARAP%3B when associated with A-156. M->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:24191030,ECO:0000269|PubMed:26043688;Dbxref=PMID:24191030,PMID:26043688	
Q9NT62	UniProtKB	Mutagenesis	160	160	.	.	.	Note=Strongly decreases affinity for the complex ATG12:ATG5:ATG16L1. Affects modestly the ATG3-ATG7 interaction. Reduces the thioester-linkage formation with GABARAP. Y->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:24191030,ECO:0000269|PubMed:26043688;Dbxref=PMID:24191030,PMID:26043688	
Q9NT62	UniProtKB	Mutagenesis	161	161	.	.	.	Note=Strongly decreases affinity for the complex ATG12:ATG5:ATG16L1. E->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:24191030;Dbxref=PMID:24191030	
Q9NT62	UniProtKB	Mutagenesis	167	167	.	.	.	Note=Weakens the ATG3-ATG7 interaction. Reduces the thioester-linkage formation with GABARAP. E->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26043688;Dbxref=PMID:26043688	
Q9NT62	UniProtKB	Mutagenesis	169	169	.	.	.	Note=Weakens the ATG3-ATG7 interaction. Severly reduces the thioester-linkage formation with GABARAP. D->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26043688;Dbxref=PMID:26043688	
Q9NT62	UniProtKB	Mutagenesis	171	171	.	.	.	Note=Weakens the ATG3-ATG7 interaction. Reduces the thioester-linkage formation with GABARAP. A->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26043688;Dbxref=PMID:26043688	
Q9NT62	UniProtKB	Mutagenesis	172	172	.	.	.	Note=Weakens the ATG3-ATG7 interaction. Significantly reduces the thioester-linkage formation with GABARAP. T->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26043688;Dbxref=PMID:26043688	
Q9NT62	UniProtKB	Mutagenesis	173	173	.	.	.	Note=Weakens the ATG3-ATG7 interaction. Significantly reduces the thioester-linkage formation with GABARAP. L->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26043688;Dbxref=PMID:26043688	
Q9NT62	UniProtKB	Mutagenesis	264	264	.	.	.	Note=Does not affect interaction with ATG12:ATG5. Impairs MAP1LC3A lipidation. Impairs MAP1LC3B lipidation. Impairs ATG3:ATG8-like proteins thioester complex formation. C->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:24191030,ECO:0000269|PubMed:37252361;Dbxref=PMID:24191030,PMID:37252361	
Q9NT62	UniProtKB	Mutagenesis	264	264	.	.	.	Note=Instead of the formation of an intermediate complex with a thiol ester bond between ATG3 (E2-like enzyme) and GABARAPL1/APG8L (substrate)%2C a stable complex with an O-ester bond is formed. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11825910;Dbxref=PMID:11825910	
Q9NT62	UniProtKB	Helix	157	163	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4NAW