Reviewed,
UniProtKB/Swiss-Prot Q9NT62 (ATG3_HUMAN)
Last modified
January 19, 2010.
Version 65.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Autophagy-related protein 3 Alternative name(s): APG3-like Short name=hApg3 Protein PC3-96 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 314 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy. |
| Subunit structure | Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Ref.1 |
| Subcellular location | |
| Tissue specificity | Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. Ref.1 |
| Sequence similarities | Belongs to the ATG3 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Autophagy Protein transport Transport Ubl conjugation pathway |
| Cellular component | Cytoplasm |
| Coding sequence diversity | Alternative splicing |
| PTM | Acetylation Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | autophagic vacuole assembly Inferred from sequence or structural similarity. Source: UniProtKB modification-dependent protein catabolic processInferred from electronic annotation. Source: UniProtKB-KW protein targeting to membraneInferred from sequence or structural similarity. Source: UniProtKB protein ubiquitination Ref.1Inferred from direct assay. Source: UniProtKB |
| Cellular component | cytoplasmic ubiquitin ligase complex Ref.1 Inferred from physical interaction. Source: MGI cytosol Ref.1Inferred from direct assay. Source: UniProtKB |
| Molecular function | enzyme binding Ref.1 Inferred from physical interaction. Source: UniProtKB small conjugating protein ligase activity Ref.1Inferred from direct assay. Source: MGI |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q9NT62-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q9NT62-2) The sequence of this isoform differs from the canonical sequence as follows: 290-314: LLIFLKFVQAVIPTIEYDYTRHFTM → PSLYVRLVAKWLLTIFFLRNLV | ||||||
| Note: No experimental confirmation available. Ref.4 (AAH02830) isoform 2 sequence differs from that shown due to a frameshift in position 311. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 314 | 314 | Autophagy-related protein 3 | PRO_0000213569 | |||||
Sites | |||||||||
| Active site | 264 | 1 | Glycyl thioester intermediate Potential | ||||||
Amino acid modifications | |||||||||
| Modified residue | 1 | 1 | N-acetylmethionine Ref.8 | ||||||
| Modified residue | 18 | 1 | Phosphotyrosine Ref.6 | ||||||
Natural variations | |||||||||
| Alternative sequence | 290 – 314 | 25 | LLIFL…RHFTM → PSLYVRLVAKWLLTIFFLRN LV in isoform 2. | VSP_013037 | |||||
Experimental info | |||||||||
| Mutagenesis | 264 | 1 | C → S: Instead of the formation of an intermediate complex with a thiol ester bond between ATG3 (E2-like enzyme) and GABARAPL1/APG8L (substrate), a stable complex with an O-ester bond is formed. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p." Tanida I., Tanida-Miyake E., Komatsu M., Ueno T., Kominami E. J. Biol. Chem. 277:13739-13744(2002) [PubMed: 11825910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, MUTAGENESIS OF CYS-264, SUBCELLULAR LOCATION, CHARACTERIZATION, INTERACTION WITH ATG7 AND ATG12. Tissue: Brain. |
| [2] | "Cloning and characterization of human PC3-96 gene." Wu B.X., Li Y., Laser M., Crosson C.E., Hazard E.S. III, Ma J.X. Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Retina. |
| [3] | "The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Lung and Lymph. |
| [5] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 118-314 (ISOFORM 2). |
| [6] | "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed: 15592455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-18, MASS SPECTROMETRY. |
| [7] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [8] | "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, MASS SPECTROMETRY. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AB079384 mRNA. Translation: BAB90843.1. AF202092 mRNA. Translation: AAG35611.1. AL137515 mRNA. Translation: CAB70781.1. BC002830 mRNA. Translation: AAH02830.1. Frameshift. BC024221 mRNA. Translation: AAH24221.1. AK025778 mRNA. Translation: BAB15237.1. Different initiation. |
| IPI | IPI00022254. IPI00449201. |
| PIR | T46276. |
| RefSeq | NP_071933.2. |
| UniGene | Hs.477126 |
3D structure databases | |
| SMR | Q9NT62. Positions 27-309. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q9NT62. 6 interactions. |
| STRING | Q9NT62. |
PTM databases | |
| PhosphoSite | Q9NT62. |
Proteomic databases | |
| PRIDE | Q9NT62. |
Genome annotation databases | |
| Ensembl | ENST00000283290; ENSP00000283290; ENSG00000144848; Homo sapiens. [Genome view] |
| GeneID | 64422. |
| KEGG | hsa:64422. |
| UCSC | uc003dzc.2. human. uc003dzd.1. human. |
Organism-specific databases | |
| CTD | 64422. |
| GeneCards | GC03M113735. |
| H-InvDB | HIX0003548. |
| HGNC | HGNC:20962. ATG3. |
| MIM | 609606. gene. |
| PharmGKB | PA134883444. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG10145. |
| HOGENOM | HBG602769. |
| HOVERGEN | Q9NT62. |
| InParanoid | Q9NT62. |
| OMA | YHNTGIA. |
| OrthoDB | EOG9WWV4X. |
| PhylomeDB | Q9NT62. |
Gene expression databases | |
| ArrayExpress | Q9NT62. |
| Bgee | Q9NT62. |
| CleanEx | HS_ATG3. |
| Genevestigator | Q9NT62. |
| GermOnline | ENSG00000144848. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR007135. Autophagy-rel_prot_3. IPR019461. Autophagy-rel_prot_3_C. IPR007134. Autophagy-rel_prot_3_N. [Graphical view] |
| Pfam | PF03987. Autophagy_act_C. 1 hit. PF10381. Autophagy_Cterm. 1 hit. PF03986. Autophagy_N. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 66413. |
| SOURCE | Search... |
Entry information
| Entry name | ATG3_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9NT62 Secondary accession number(s): Q6PKC5, Q9H6L9 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 3 Human chromosome 3: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

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