Q9NSY0 (NRBP2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nuclear receptor-binding protein 2

Alternative name(s):
Transformation-related gene 16 protein
Short name=TRG-16

Gene names

Name:	NRBP2
ORF Names:	PP9320, TRG16

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May regulate apoptosis of neural progenitor cells during their differentiation By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	The protein kinase domain is predicted to be catalytically inactive.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.
Sequence caution	The sequence AAI13874.1 differs from that shown. Reason: Probable cloning artifact. The sequence CAB70864.1 differs from that shown. Reason: Wrong choice of CDS.

Ontologies

Keywords
Biological process	Neurogenesis
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of macroautophagy Inferred from mutant phenotype PubMed 22354037. Source: BHF-UCL negative regulation of neuron apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB neuron differentiation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	transferase activity, transferring phosphorus-containing groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.4 Ref.5 PubMed 11230166 (identifier: Q9NSY0-3)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.1 Ref.3 (identifier: Q9NSY0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-243: Missing.
     244-280: MCALEMAVLE...RHSLSDPNMR → MVLQSQNPAV...HHQPLLPVPQ

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9NSY0-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-243: Missing.
     244-280: MCALEMAVLE...RHSLSDPNMR → MVLQSQNPAV...HHQPLLPVPQ
     461-501: TDSAQDLASE...TFLKYRGTQA → SRLGRGLGRR...LPQVPWDPGL

Note: No experimental confirmation available.

Isoform 4 (identifier: Q9NSY0-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-48: Missing.
     149-171: AWKRWCTQILSALSFLHACSPPI → VWGAGWGSHGDRTGLGQPRGLGW
     172-501: Missing.

Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Nuclear receptor-binding protein 2		PRO_0000225608
Regions
	Domain	38 – 306	269	Protein kinase
Natural variations
	Alternative sequence	1 – 243	243	Missing in isoform 2 and isoform 3.		VSP_039308
	Alternative sequence	1 – 48	48	Missing in isoform 4.		VSP_039311
	Alternative sequence	149 – 171	23	AWKRW…CSPPI → VWGAGWGSHGDRTGLGQPRG LGW in isoform 4.		VSP_039312
	Alternative sequence	172 – 501	330	Missing in isoform 4.		VSP_039313
	Alternative sequence	244 – 280	37	MCALE…DPNMR → MVLQSQNPAVLKLRGPGPGL AAAAGTPPYGHPCMSCVSLR RDMGNSGATSPRTAGPEQRA LAHHQPLLPVPQ in isoform 2 and isoform 3.		VSP_039309
	Alternative sequence	461 – 501	41	TDSAQ…RGTQA → SRLGRGLGRRAVTPGGGWAA ASCLALRHCLSGQRPGPRLG ARALWLPPRGRPDEAGRLPG EHLPQVPWDPGL in isoform 3.		VSP_039310
Experimental info
Isoform 3:
	Sequence conflict	10	1	V → A in AAQ18040. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 15, 2010. Version 2. Checksum: B3F246DBA7E2AC6C	FASTA	501	57,803
	10 20 30 40 50 60 MAAPEPAPRR ARERERERED ESEDESDILE ESPCGRWQKR REQVNQGNMP GLQSTFLAMD 70 80 90 100 110 120 TEEGVEVVWN ELHFGDRKAF AAHEEKIQTV FEQLVLVDHP NIVKLHKYWL DTSEACARVI 130 140 150 160 170 180 FITEYVSSGS LKQFLKKTKK NHKAMNARAW KRWCTQILSA LSFLHACSPP IIHGNLTSDT 190 200 210 220 230 240 IFIQHNGLIK IGSVWHRIFS NALPDDLRSP IRAEREELRN LHFFPPEYGE VADGTAVDIF 250 260 270 280 290 300 SFGMCALEMA VLEIQTNGDT RVTEEAIARA RHSLSDPNMR EFILCCLARD PARRPSAHSL 310 320 330 340 350 360 LFHRVLFEVH SLKLLAAHCF IQHQYLMPEN VVEEKTKAMD LHAVLAELPR PRRPPLQWRY 370 380 390 400 410 420 SEVSFMELDK FLEDVRNGIY PLMNFAATRP LGLPRVLAPP PEEVQKAKTP TPEPFDSETR 430 440 450 460 470 480 KVIQMQCNLE RSEDKARWHL TLLLVLEDRL HRQLTYDLLP TDSAQDLASE LVHYGFLHED 490 500 DRMKLAAFLE STFLKYRGTQ A
	Isoform 2 [UniParc]. Checksum: B37F0F45BD8AC99B	FASTA	293	33,141
	10 20 30 40 50 60 MVLQSQNPAV LKLRGPGPGL AAAAGTPPYG HPCMSCVSLR RDMGNSGATS PRTAGPEQRA 70 80 90 100 110 120 LAHHQPLLPV PQEFILCCLA RDPARRPSAH SLLFHRVLFE VHSLKLLAAH CFIQHQYLMP 130 140 150 160 170 180 ENVVEEKTKA MDLHAVLAEL PRPRRPPLQW RYSEVSFMEL DKFLEDVRNG IYPLMNFAAT 190 200 210 220 230 240 RPLGLPRVLA PPPEEVQKAK TPTPEPFDSE TRKVIQMQCN LERSEDKARW HLTLLLVLED 250 260 270 280 290 RLHRQLTYDL LPTDSAQDLA SELVHYGFLH EDDRMKLAAF LESTFLKYRG TQA
	Isoform 3 [UniParc]. Checksum: 45EDFCB9AFCB5660	FASTA	324	36,072
	10 20 30 40 50 60 MVLQSQNPAV LKLRGPGPGL AAAAGTPPYG HPCMSCVSLR RDMGNSGATS PRTAGPEQRA 70 80 90 100 110 120 LAHHQPLLPV PQEFILCCLA RDPARRPSAH SLLFHRVLFE VHSLKLLAAH CFIQHQYLMP 130 140 150 160 170 180 ENVVEEKTKA MDLHAVLAEL PRPRRPPLQW RYSEVSFMEL DKFLEDVRNG IYPLMNFAAT 190 200 210 220 230 240 RPLGLPRVLA PPPEEVQKAK TPTPEPFDSE TRKVIQMQCN LERSEDKARW HLTLLLVLED 250 260 270 280 290 300 RLHRQLTYDL LPSRLGRGLG RRAVTPGGGW AAASCLALRH CLSGQRPGPR LGARALWLPP 310 320 RGRPDEAGRL PGEHLPQVPW DPGL
	Isoform 4 [UniParc]. Checksum: A24C99DA3D502C1C	FASTA	123	13,910
	10 20 30 40 50 60 MPGLQSTFLA MDTEEGVEVV WNELHFGDRK AFAAHEEKIQ TVFEQLVLVD HPNIVKLHKY 70 80 90 100 110 120 WLDTSEACAR VIFITEYVSS GSLKQFLKKT KKNHKAMNAR VWGAGWGSHG DRTGLGQPRG LGW

References

[1]	"Identification of a human transforming gene." Kim J.W. Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Cerebellum.
[3]	"Large-scale cDNA transfection screening for genes related to cancer development and progression." Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 208-501 (ISOFORM 1). Tissue: Testis.
[5]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 50-501 (ISOFORM 1).
[7]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 149-501 (ISOFORM 1).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY277601 mRNA. Translation: AAQ18040.1. AK293943 mRNA. Translation: BAG57320.1. AF318376 mRNA. Translation: AAL55883.1. AL137662 mRNA. Translation: CAB70864.1. Sequence problems. AL834530 mRNA. Translation: CAD39186.1. AC105049 Genomic DNA. No translation available. BC113873 mRNA. Translation: AAI13874.1. Sequence problems. CR457350 mRNA. Translation: CAG33631.1.
IPI	IPI00168489. IPI00383275. IPI00964924. IPI00965220.
PIR	T46491.
RefSeq	NP_848659.2. NM_178564.3.
UniGene	Hs.521926.
3D structure databases
ProteinModelPortal	Q9NSY0.
SMR	Q9NSY0. Positions 35-303.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000330271.
PTM databases
PhosphoSite	Q9NSY0.
Polymorphism databases
DMDM	298286835.
Proteomic databases
PaxDb	Q9NSY0.
PRIDE	Q9NSY0.
Protocols and materials databases
DNASU	340371.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000442628; ENSP00000414055; ENSG00000185189. ENST00000568732; ENSP00000457135; ENSG00000260533.
GeneID	340371.
KEGG	hsa:340371.
UCSC	uc003yzw.3. human. uc003yzy.3. human. uc010mfl.3. human.
Organism-specific databases
CTD	340371.
GeneCards	GC08M144917.
H-InvDB	HIX0021894.
HGNC	HGNC:19339. NRBP2.
HPA	CAB020944. HPA029052.
neXtProt	NX_Q9NSY0.
PharmGKB	PA134891143.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG282910.
HOGENOM	HOG000267147.
HOVERGEN	HBG057400.
InParanoid	Q9NSY0.
KO	K08875.
OMA	QVEHPNI.
OrthoDB	EOG4PNXGS.
Gene expression databases
ArrayExpress	Q9NSY0.
Bgee	Q9NSY0.
CleanEx	HS_NRBP2.
Genevestigator	Q9NSY0.
GermOnline	ENSG00000185189. Homo sapiens.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS50011. PROTEIN_KINASE_DOM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	340371.
NextBio	97840.

Entry information

Entry name

NRBP2_HUMAN

Accession

Primary (citable) accession number: Q9NSY0
Secondary accession number(s): B4DF59 , Q2HIX8, Q597G7, Q8NCX8, Q8WYS5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	June 15, 2010
Last modified:	May 1, 2013
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.