Q9NSP4 (CENPM_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Centromere protein M
Short name=CENP-M

Alternative name(s):
Interphase centromere complex protein 39
Proliferation-associated nuclear element protein 1

Gene names

Name:	CENPM
Synonyms:	C22orf18, ICEN39, PANE1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	180 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Ref.9
Subunit structure	Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and MLF1IP/CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Ref.10
Subcellular location	Nucleus. Cytoplasm. Chromosome › centromere › kinetochore. Note: Nuclear in non-confluent cells and cytoplasmic in confluent or dividing cells By similarity. Localizes in the kinetochore domain of centromeres. Ref.8 Ref.9
Tissue specificity	Isoform 3 is highly expressed in spleen, and intermediately in heart, prostate and ovary. Isoform 3 is highly expressed in resting CD19 B-cells and B-lineage chronic lymphocytic leukemia (B-CLL) cells and weakly expressed in activated B-cells. Isoform 1 is selectively expressed in activated CD19 cells and weakly in resting CD19 B-cells.

Ontologies

Keywords
Cellular component	Centromere Chromosome Cytoplasm Kinetochore Nucleus
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitotic prometaphase Traceable author statement. Source: Reactome
Cellular_component	condensed chromosome kinetochore Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Traceable author statement. Source: Reactome nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NSP4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NSP4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     104-132: AGRESHCSIHRHTVVKLAHTYQSPLLYCD → GKYVPRLLLPTPSQGKAGAAVGFLLRHPG
     133-180: Missing.

Note: Due to intron retention. No experimental confirmation available.

Isoform 3 (identifier: Q9NSP4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-122: Missing.
     123-134: TYQSPLLYCDLE → MGRVWDLPGVLK

Isoform 4 (identifier: Q9NSP4-4)

The sequence of this isoform differs from the canonical sequence as follows:
     104-179: AGRESHCSIH...RSSEGPSLED → GGR

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 180	180	Centromere protein M		PRO_0000058224
Natural variations
	Alternative sequence	1 – 122	122	Missing in isoform 3.		VSP_017726
	Alternative sequence	104 – 179	76	AGRES…PSLED → GGR in isoform 4.		VSP_046189
	Alternative sequence	104 – 132	29	AGRES…LLYCD → GKYVPRLLLPTPSQGKAGAA VGFLLRHPG in isoform 2.		VSP_010259
	Alternative sequence	123 – 134	12	TYQSP…YCDLE → MGRVWDLPGVLK in isoform 3.		VSP_017725
	Alternative sequence	133 – 180	48	Missing in isoform 2.		VSP_010260

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 37DB044092BF63F4	FASTA	180	19,737
	10 20 30 40 50 60 MSVLRPLDKL PGLNTATILL VGTEDALLQQ LADSMLKEDC ASELKVHLAK SLPLPSSVNR 70 80 90 100 110 120 PRIDLIVFVV NLHSKYSLQN TEESLRHVDA SFFLGKVCFL ATGAGRESHC SIHRHTVVKL 130 140 150 160 170 180 AHTYQSPLLY CDLEVEGFRA TMAQRLVRVL QICAGHVPGV SALNLLSLLR SSEGPSLEDL
	Isoform 2 [UniParc]. Checksum: 3A8E77D553024B38	FASTA	132	14,287
	10 20 30 40 50 60 MSVLRPLDKL PGLNTATILL VGTEDALLQQ LADSMLKEDC ASELKVHLAK SLPLPSSVNR 70 80 90 100 110 120 PRIDLIVFVV NLHSKYSLQN TEESLRHVDA SFFLGKVCFL ATGGKYVPRL LLPTPSQGKA 130 GAAVGFLLRH PG
	Isoform 3 [UniParc]. Checksum: D5B353502075D069	FASTA	58	6,257
	10 20 30 40 50 MGRVWDLPGV LKVEGFRATM AQRLVRVLQI CAGHVPGVSA LNLLSLLRSS EGPSLEDL
	Isoform 4 [UniParc]. Checksum: 4FBAB92E26A66A1F	FASTA	107	11,687
	10 20 30 40 50 60 MSVLRPLDKL PGLNTATILL VGTEDALLQQ LADSMLKEDC ASELKVHLAK SLPLPSSVNR 70 80 90 100 PRIDLIVFVV NLHSKYSLQN TEESLRHVDA SFFLGKVCFL ATGGGRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The PANE1 gene encodes a novel human minor histocompatibility antigen that is selectively expressed in B-lymphoid cells and B-CLL." Brickner A.G., Evans A.M., Mito J.K., Xuereb S.M., Feng X., Nishida T., Fairfull L., Ferrell R.E., Foon K.A., Hunt D.F., Shabanowitz J., Engelhard V.H., Riddell S.R., Warren E.H. Blood 107:3779-3786(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), IDENTIFICATION BY MASS SPECTROMETRY.
[2]	"Reevaluating human gene annotation: a second-generation analysis of chromosome 22." Collins J.E., Goward M.E., Cole C.G., Smink L.J., Huckle E.J., Knowles S., Bye J.M., Beare D.M., Dunham I. Genome Res. 13:27-36(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Thalamus.
[4]	"A genome annotation-driven approach to cloning the human ORFeome." Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I. Genome Biol. 5:R84.1-R84.11(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The DNA sequence of human chromosome 22." Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M. , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H. Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4). Tissue: Cervix, Lymph and Melanoma.
[8]	"The proliferation associated nuclear element (PANE1) is conserved between mammals and fish and preferentially expressed in activated lymphoid cells." Bierie B., Edwin M., Melenhorst J.J., Hennighausen L. Gene Expr. Patterns 4:389-395(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[9]	"Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells." Izuta H., Ikeno M., Suzuki N., Tomonaga T., Nozaki N., Obuse C., Kisu Y., Goshima N., Nomura F., Nomura N., Yoda K. Genes Cells 11:673-684(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[10]	"The human CENP-A centromeric nucleosome-associated complex." Foltz D.R., Jansen L.E.T., Black B.E., Bailey A.O., Yates J.R. III, Cleveland D.W. Nat. Cell Biol. 8:458-469(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE CENPA-NAC COMPLEX WITH CENPA; CENPC; CENPH; CENPN; CENPT AND CENPU.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EU035297 mRNA. Translation: ABS84666.1. AL160131 mRNA. Translation: CAB77147.1. AK123479 mRNA. Translation: BAC85624.1. CR456399 mRNA. Translation: CAG30285.1. CR457392 mRNA. Translation: CAG33673.1. Z99716 Genomic DNA. Translation: CAI41691.1. BC000705 mRNA. Translation: AAH00705.1. BC007495 mRNA. Translation: AAH07495.1. BU194618 mRNA. No translation available.
IPI	IPI00031566. IPI00410143. IPI00739757. IPI00938104.
RefSeq	NP_001002876.1. NM_001002876.1. NP_001103685.1. NM_001110215.1. NP_076958.1. NM_024053.3.
UniGene	Hs.208912.
3D structure databases
ProteinModelPortal	Q9NSP4.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000215980.
Polymorphism databases
DMDM	47117330.
Proteomic databases
PaxDb	Q9NSP4.
PRIDE	Q9NSP4.
Protocols and materials databases
DNASU	79019.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000215980; ENSP00000215980; ENSG00000100162. ENST00000407253; ENSP00000384743; ENSG00000100162. ENST00000472374; ENSP00000430624; ENSG00000100162.
GeneID	79019.
KEGG	hsa:79019.
UCSC	uc003bbn.3. human. uc003bbp.1. human. uc010gyq.3. human.
Organism-specific databases
CTD	79019.
GeneCards	GC22M042334.
HGNC	HGNC:18352. CENPM.
HPA	CAB006330.
MIM	610152. gene.
neXtProt	NX_Q9NSP4.
PharmGKB	PA25885.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG25434.
HOGENOM	HOG000261677.
HOVERGEN	HBG081086.
InParanoid	Q9NSP4.
KO	K11505.
OMA	FCDLEVE.
OrthoDB	EOG4R23W7.
PhylomeDB	Q9NSP4.
Enzyme and pathway databases
Reactome	REACT_115566. Cell Cycle. REACT_21300. Mitotic M-M/G1 phases.
Gene expression databases
ArrayExpress	Q9NSP4.
Bgee	Q9NSP4.
CleanEx	HS_CENPM.
Genevestigator	Q9NSP4.
GermOnline	ENSG00000100162. Homo sapiens.
Family and domain databases
InterPro	IPR020987. Centromere_Cenp-M. [Graphical view]
Pfam	PF11111. CENP-M. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	79019.
NextBio	67686.
SOURCE	Search...

Entry information

Entry name

CENPM_HUMAN

Accession

Primary (citable) accession number: Q9NSP4
Secondary accession number(s): A7LM22, B1AHQ9, Q6I9W3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	October 1, 2000
Last modified:	April 3, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.