Reviewed, UniProtKB/Swiss-Prot Q9NSK0 (KLC4_HUMAN)

Names and origin

Protein names

Recommended name:
    Kinesin light chain 4
      Short name=KLC 4
Alternative name(s):
    Kinesin-like protein 8

Gene names

Name:	KLC4
Synonyms:	KNSL8

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	619 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity By similarity.
Subunit structure	Oligomeric complex composed of two heavy chains and two light chains By similarity.
Sequence similarities	Belongs to the kinesin light chain family. Contains 7 TPR repeats.

Ontologies

Keywords
Cellular component	Microtubule
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Repeat TPR repeat
Molecular function	Motor protein
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	kinesin complex Inferred from electronic annotation. Source: InterPro microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	microtubule motor activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
YWHAG	P61981	1	EBI-949319,EBI-359832
YWHAQ	P27348	1	EBI-949319,EBI-359854
YWHAZ	P63104	1	EBI-949319,EBI-347088

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NSK0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NSK0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-87: Missing.
     419-422: DDHK → EPAP
     423-619: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 619	619	Kinesin light chain 4		PRO_0000215097
Regions
	Repeat	55 – 88	34	TPR 1
	Repeat	211 – 244	34	TPR 2
	Repeat	253 – 286	34	TPR 3
	Repeat	295 – 328	34	TPR 4
	Repeat	337 – 370	34	TPR 5
	Repeat	379 – 412	34	TPR 6
	Repeat	464 – 497	34	TPR 7
	Coiled coil	32 – 150	119	Potential
Amino acid modifications
	Modified residue	74	1	Phosphoserine Ref.6
	Modified residue	83	1	Phosphoserine Ref.6
	Modified residue	93	1	Phosphoserine Ref.6
	Modified residue	100	1	Phosphoserine Ref.6
	Modified residue	174	1	Phosphoserine By similarity
	Modified residue	460	1	Phosphoserine Ref.5 Ref.8 Ref.9
	Modified residue	566	1	Phosphoserine By similarity
	Modified residue	590	1	Phosphoserine Ref.9 Ref.7
Natural variations
	Alternative sequence	1 – 87	87	Missing in isoform 2.		VSP_021012
	Alternative sequence	419 – 422	4	DDHK → EPAP in isoform 2.		VSP_021013
	Alternative sequence	423 – 619	197	Missing in isoform 2.		VSP_021014
	Natural variant	72	1	R → H: dbSNP rs11558979. Ref.1		VAR_049708
Experimental info
	Sequence conflict	54	1	L → LQTIECL in BAC87179. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 17, 2006. Version 3. Checksum: 32DFC2A9A91B5574	FASTA	619	68,640
	10 20 30 40 50 60 MSGLVLGQRD EPAGHRLSQE EILGSTRLVS QGLEALRSEH QAVLQSLSQT IECLQQGGHE 70 80 90 100 110 120 EGLVHEKARQ LRRSMENIEL GLSEAQVMLA LASHLSTVES EKQKLRAQVR RLCQENQWLR 130 140 150 160 170 180 DELAGTQQRL QRSEQAVAQL EEEKKHLEFL GQLRQYDEDG HTSEEKEGDA TKDSLDDLFP 190 200 210 220 230 240 NEEEEDPSNG LSRGQGATAA QQGGYEIPAR LRTLHNLVIQ YAAQGRYEVA VPLCKQALED 250 260 270 280 290 300 LERTSGRGHP DVATMLNILA LVYRDQNKYK EAAHLLNDAL SIRESTLGPD HPAVAATLNN 310 320 330 340 350 360 LAVLYGKRGK YKEAEPLCQR ALEIREKVLG TNHPDVAKQL NNLALLCQNQ GKYEAVERYY 370 380 390 400 410 420 QRALAIYEGQ LGPDNPNVAR TKNNLASCYL KQGKYAEAET LYKEILTRAH VQEFGSVDDD 430 440 450 460 470 480 HKPIWMHAEE REEMSKSRHH EGGTPYAEYG GWYKACKVSS PTVNTTLRNL GALYRRQGKL 490 500 510 520 530 540 EAAETLEECA LRSRRQGTDP ISQTKVAELL GESDGRRTSQ EGPGDSVKFE GGEDASVAVE 550 560 570 580 590 600 WSGDGSGTLQ RSGSLGKIRD VLRRSSELLV RKLQGTEPRP SSSNMKRAAS LNYLNQPSAA 610 PLQVSRGLSA STMDLSSSS
	Isoform 2. Checksum: D2CA951E060DD9E7	FASTA	335	37,604
	10 20 30 40 50 60 MLALASHLST VESEKQKLRA QVRRLCQENQ WLRDELAGTQ QRLQRSEQAV AQLEEEKKHL 70 80 90 100 110 120 EFLGQLRQYD EDGHTSEEKE GDATKDSLDD LFPNEEEEDP SNGLSRGQGA TAAQQGGYEI 130 140 150 160 170 180 PARLRTLHNL VIQYAAQGRY EVAVPLCKQA LEDLERTSGR GHPDVATMLN ILALVYRDQN 190 200 210 220 230 240 KYKEAAHLLN DALSIRESTL GPDHPAVAAT LNNLAVLYGK RGKYKEAEPL CQRALEIREK 250 260 270 280 290 300 VLGTNHPDVA KQLNNLALLC QNQGKYEAVE RYYQRALAIY EGQLGPDNPN VARTKNNLAS 310 320 330 CYLKQGKYAE AETLYKEILT RAHVQEFGSV DEPAP

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT HIS-72. Tissue: Brain and Small intestine.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Amygdala and Oesophageal carcinoma.
[3]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Prostate and Skin.
[5]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-460, MASS SPECTROMETRY. Tissue: Epithelium.
[6]	"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-74; SER-83; SER-93 AND SER-100, MASS SPECTROMETRY.
[7]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-590, MASS SPECTROMETRY.
[8]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-460, MASS SPECTROMETRY.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-460 AND SER-590, MASS SPECTROMETRY.
[10]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK055293 mRNA. Translation: BAG51496.1. AK127018 mRNA. Translation: BAC86788.1. AK127894 mRNA. Translation: BAC87179.1. AL162078 mRNA. Translation: CAB82411.1. AL355385 Genomic DNA. Translation: CAI13781.1. BC080637 mRNA. Translation: AAH80637.1. BC103727 mRNA. Translation: AAI03728.1. BX640717 mRNA. Translation: CAE45836.1.
IPI	IPI00398812. IPI00788630.
PIR	T47147.
RefSeq	NP_958929.1. NP_958930.1.
UniGene	Hs.655123
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NSK0. 6 interactions.
PTM databases
PhosphoSite	Q9NSK0.
Proteomic databases
PRIDE	Q9NSK0.
Genome annotation databases
Ensembl	ENSG00000137171. Homo sapiens. [Contig view]
GeneID	89953.
UCSC	uc003otv.1. human.
Organism-specific databases
GeneCards	GC06P043136.
HGNC	HGNC:21624. KLC4.
PharmGKB	PA134934134.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9NSK0.
HOVERGEN	Q9NSK0.
Gene expression databases
ArrayExpress	Q9NSK0.
Bgee	Q9NSK0.
CleanEx	HS_KLC4.
GermOnline	ENSG00000137171. Homo sapiens.
Family and domain databases
InterPro	IPR002151. Kinesin_light. IPR015792. Kinesin_light_repeat. IPR015390. Rabaptin_Rab5-bd. IPR001440. TPR-1. IPR011990. TPR-like_helical. IPR013026. TPR_region. IPR019734. TPR_repeat. [Graphical view]
Gene3D	G3DSA:1.25.40.10. TPR-like_helical. 1 hit.
Pfam	PF09311. Rab5-bind. 1 hit. PF00515. TPR_1. 4 hits. [Graphical view]
PRINTS	PR00381. KINESINLIGHT.
SMART	SM00028. TPR. 4 hits. [Graphical view]
PROSITE	PS01160. KINESIN_LIGHT. 3 hits. PS50005. TPR. 6 hits. PS50293. TPR_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	76452.

Entry information

Entry name

KLC4_HUMAN

Accession

Primary (citable) accession number: Q9NSK0
Secondary accession number(s): B3KNY4, Q66K28

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	October 17, 2006
Last modified:	July 7, 2009
This is version 73 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)