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Protein

SAM domain-containing protein SAMSN-1

Gene

SAMSN1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negative regulator of B-cell activation. Down-regulates cell proliferation (in vitro). Promotes RAC1-dependent membrane ruffle formation and reorganization of the actin cytoskeleton. Regulates cell spreading and cell polarization. Stimulates HDAC1 activity. Regulates LYN activity by modulating its tyrosine phosphorylation (By similarity).By similarity1 Publication

GO - Molecular functioni

  • phosphotyrosine binding Source: MGI
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000155307-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
SAM domain-containing protein SAMSN-1
Alternative name(s):
Hematopoietic adaptor containing SH3 and SAM domains 1
Nash1
SAM domain, SH3 domain and nuclear localization signals protein 1
SH3-SAM adaptor protein
Gene namesi
Name:SAMSN1
Synonyms:HACS1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 21

Organism-specific databases

HGNCiHGNC:10528. SAMSN1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Ensembl
  • nucleus Source: UniProtKB
  • ruffle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi64092.
OpenTargetsiENSG00000155307.
PharmGKBiPA34939.

Polymorphism and mutation databases

BioMutaiSAMSN1.
DMDMi12230638.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000975741 – 373SAM domain-containing protein SAMSN-1Add BLAST373

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei23PhosphoserineCombined sources1
Modified residuei34PhosphoserineCombined sources1
Modified residuei74PhosphoserineCombined sources1
Modified residuei76PhosphothreonineCombined sources1
Modified residuei90PhosphoserineCombined sources1
Modified residuei119PhosphoserineCombined sources1
Modified residuei160PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9NSI8.
MaxQBiQ9NSI8.
PaxDbiQ9NSI8.
PeptideAtlasiQ9NSI8.
PRIDEiQ9NSI8.

PTM databases

iPTMnetiQ9NSI8.
PhosphoSitePlusiQ9NSI8.

Expressioni

Tissue specificityi

Detected in peripheral blood B-cells (at protein level). Detected in spleen, liver and peripheral blood.2 Publications

Inductioni

Up-regulated in peripheral blood B-cells by IL4, IL13 and by CD40 stimulation.1 Publication

Gene expression databases

BgeeiENSG00000155307.
CleanExiHS_SAMSN1.
ExpressionAtlasiQ9NSI8. baseline and differential.
GenevisibleiQ9NSI8. HS.

Organism-specific databases

HPAiHPA010645.
HPA017055.

Interactioni

Subunit structurei

Interacts with FASLG. Interacts with phosphotyrosine containing proteins. Interacts (via SH3 domain) with CTTN. Interacts (phosphorylated at Ser-23) with YWHAB, YWHAE, YWHAG, YWHAH, YWHAZ and SFN. Interacts directly with SAP30 and HDAC1. Identified in a complex with SAP30 and HDAC1 (By similarity).By similarity

GO - Molecular functioni

  • phosphotyrosine binding Source: MGI

Protein-protein interaction databases

BioGridi122054. 12 interactors.
IntActiQ9NSI8. 2 interactors.
STRINGi9606.ENSP00000383411.

Structurei

Secondary structure

1373
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi167 – 170Combined sources4
Beta strandi181 – 183Combined sources3
Beta strandi191 – 196Combined sources6
Beta strandi203 – 207Combined sources5
Beta strandi210 – 214Combined sources5
Helixi216 – 218Combined sources3
Beta strandi219 – 221Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KEANMR-A162-224[»]
ProteinModelPortaliQ9NSI8.
SMRiQ9NSI8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NSI8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini166 – 222SH3Add BLAST57
Domaini241 – 305SAMPROSITE-ProRule annotationAdd BLAST65

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi20 – 25Important for interaction with 14-3-3 proteinsBy similarity6

Sequence similaritiesi

Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.Curated

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG4384. Eukaryota.
ENOG410ZEDF. LUCA.
GeneTreeiENSGT00390000014672.
HOGENOMiHOG000049202.
HOVERGENiHBG000461.
InParanoidiQ9NSI8.
OMAiGPYSGPF.
OrthoDBiEOG091G0BS1.
PhylomeDBiQ9NSI8.
TreeFamiTF350709.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
InterProiIPR021090. rSAM/SH3_domain-containing.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF07647. SAM_2. 1 hit.
PF07653. SH3_2. 1 hit.
PF12485. SLY. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS50105. SAM_DOMAIN. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NSI8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLKRKPSNVS EKEKHQKPKR SSSFGNFDRF RNNSLSKPDD STEAHEGDPT
60 70 80 90 100
NGSGEQSKTS NNGGGLGKKM RAISWTMKKK VGKKYIKALS EEKDEEDGEN
110 120 130 140 150
AHPYRNSDPV IGTHTEKVSL KASDSMDSLY SGQSSSSGIT SCSDGTSNRD
160 170 180 190 200
SFRLDDDGPY SGPFCGRARV HTDFTPSPYD TDSLKIKKGD IIDIICKTPM
210 220 230 240 250
GMWTGMLNNK VGNFKFIYVD VISEEEAAPK KIKANRRSNS KKSKTLQEFL
260 270 280 290 300
ERIHLQEYTS TLLLNGYETL EDLKDIKESH LIELNIENPD DRRRLLSAAE
310 320 330 340 350
NFLEEEIIQE QENEPEPLSL SSDISLNKSQ LDDCPRDSGC YISSGNSDNG
360 370
KEDLESENLS DMVHKIIITE PSD
Length:373
Mass (Da):41,708
Last modified:October 1, 2000 - v1
Checksum:i964B852ADBA2D777
GO
Isoform 2 (identifier: Q9NSI8-2) [UniParc]FASTAAdd to basket
Also known as: b

The sequence of this isoform differs from the canonical sequence as follows:
     19-186: Missing.

Show »
Length:205
Mass (Da):23,466
Checksum:i362B2FB490B972BE
GO
Isoform 3 (identifier: Q9NSI8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: MLKRKPSNVSEKEKHQKPK → MEIRLDTLSA...CSDMDLLHSW

Show »
Length:441
Mass (Da):49,627
Checksum:iC2757916D880F563
GO

Sequence cautioni

The sequence AAK07746 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti105R → G in BAG54155 (PubMed:14702039).Curated1
Isoform 3 (identifier: Q9NSI8-3)
Sequence conflicti20N → D in AAK07746 (PubMed:11536050).Curated1
Sequence conflicti64H → Y in AAK07746 (PubMed:11536050).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05133163G → A.Corresponds to variant rs34607574dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0081201 – 19MLKRK…HQKPK → MEIRLDTLSASLGRSSTLNN CNLEDKLAWYEGEAYMWHHW KPFPENPLWTCLDFQIAQVG PWDHCSSCIRHTRLKSSCSD MDLLHSW in isoform 3. 1 PublicationAdd BLAST19
Alternative sequenceiVSP_00811919 – 186Missing in isoform 2. 1 PublicationAdd BLAST168

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF222927 mRNA. Translation: AAG23355.1.
AF218085 mRNA. Translation: AAK07746.1. Different initiation.
AF519621 mRNA. Translation: AAM75349.1.
AK125144 mRNA. Translation: BAG54155.1.
AL163206 Genomic DNA. Translation: CAB90391.1.
AF165138 Genomic DNA. No translation available.
BC029112 mRNA. Translation: AAH29112.1.
CCDSiCCDS42906.1. [Q9NSI8-1]
CCDS58786.1. [Q9NSI8-3]
RefSeqiNP_001243299.1. NM_001256370.1. [Q9NSI8-3]
NP_001273452.1. NM_001286523.1.
NP_071419.3. NM_022136.4. [Q9NSI8-1]
UniGeneiHs.473341.
Hs.719474.

Genome annotation databases

EnsembliENST00000285670; ENSP00000285670; ENSG00000155307. [Q9NSI8-3]
ENST00000400564; ENSP00000383409; ENSG00000155307. [Q9NSI8-2]
ENST00000400566; ENSP00000383411; ENSG00000155307. [Q9NSI8-1]
GeneIDi64092.
KEGGihsa:64092.
UCSCiuc002yju.3. human. [Q9NSI8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF222927 mRNA. Translation: AAG23355.1.
AF218085 mRNA. Translation: AAK07746.1. Different initiation.
AF519621 mRNA. Translation: AAM75349.1.
AK125144 mRNA. Translation: BAG54155.1.
AL163206 Genomic DNA. Translation: CAB90391.1.
AF165138 Genomic DNA. No translation available.
BC029112 mRNA. Translation: AAH29112.1.
CCDSiCCDS42906.1. [Q9NSI8-1]
CCDS58786.1. [Q9NSI8-3]
RefSeqiNP_001243299.1. NM_001256370.1. [Q9NSI8-3]
NP_001273452.1. NM_001286523.1.
NP_071419.3. NM_022136.4. [Q9NSI8-1]
UniGeneiHs.473341.
Hs.719474.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KEANMR-A162-224[»]
ProteinModelPortaliQ9NSI8.
SMRiQ9NSI8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122054. 12 interactors.
IntActiQ9NSI8. 2 interactors.
STRINGi9606.ENSP00000383411.

PTM databases

iPTMnetiQ9NSI8.
PhosphoSitePlusiQ9NSI8.

Polymorphism and mutation databases

BioMutaiSAMSN1.
DMDMi12230638.

Proteomic databases

EPDiQ9NSI8.
MaxQBiQ9NSI8.
PaxDbiQ9NSI8.
PeptideAtlasiQ9NSI8.
PRIDEiQ9NSI8.

Protocols and materials databases

DNASUi64092.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000285670; ENSP00000285670; ENSG00000155307. [Q9NSI8-3]
ENST00000400564; ENSP00000383409; ENSG00000155307. [Q9NSI8-2]
ENST00000400566; ENSP00000383411; ENSG00000155307. [Q9NSI8-1]
GeneIDi64092.
KEGGihsa:64092.
UCSCiuc002yju.3. human. [Q9NSI8-1]

Organism-specific databases

CTDi64092.
DisGeNETi64092.
GeneCardsiSAMSN1.
H-InvDBHIX0016027.
HGNCiHGNC:10528. SAMSN1.
HPAiHPA010645.
HPA017055.
MIMi607978. gene.
neXtProtiNX_Q9NSI8.
OpenTargetsiENSG00000155307.
PharmGKBiPA34939.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4384. Eukaryota.
ENOG410ZEDF. LUCA.
GeneTreeiENSGT00390000014672.
HOGENOMiHOG000049202.
HOVERGENiHBG000461.
InParanoidiQ9NSI8.
OMAiGPYSGPF.
OrthoDBiEOG091G0BS1.
PhylomeDBiQ9NSI8.
TreeFamiTF350709.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000155307-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ9NSI8.
GenomeRNAii64092.
PROiQ9NSI8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000155307.
CleanExiHS_SAMSN1.
ExpressionAtlasiQ9NSI8. baseline and differential.
GenevisibleiQ9NSI8. HS.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
InterProiIPR021090. rSAM/SH3_domain-containing.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF07647. SAM_2. 1 hit.
PF07653. SH3_2. 1 hit.
PF12485. SLY. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS50105. SAM_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSAMN1_HUMAN
AccessioniPrimary (citable) accession number: Q9NSI8
Secondary accession number(s): B3KWJ3
, F8WAA1, Q8NFF7, Q9C041
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 21
    Human chromosome 21: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.