Reviewed,
UniProtKB/Swiss-Prot Q9NSE4 (SYIM_HUMAN)
Last modified
November 3, 2009.
Version 59.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Isoleucyl-tRNA synthetase, mitochondrial EC=6.1.1.5 Alternative name(s): Isoleucine--tRNA ligase Short name=IleRS | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) [Complete proteome] | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 1012 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). |
| Subcellular location | Mitochondrion matrix By similarity. |
| Sequence similarities | Belongs to the class-I aminoacyl-tRNA synthetase family. |
| Sequence caution | The sequence BAA91544.1 differs from that shown. Reason: Frameshift at position 879. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Protein biosynthesis |
| Cellular component | Mitochondrion |
| Coding sequence diversity | Polymorphism |
| Domain | Transit peptide |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Aminoacyl-tRNA synthetase Ligase |
| PTM | Acetylation Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro |
| Cellular component | mitochondrial matrix Inferred from Experiment. Source: Reactome |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW isoleucine-tRNA ligase activityInferred from Experiment. Source: Reactome zinc ion bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 48 | 48 | Mitochondrion Potential | ||||||
| Chain | 49 – 1012 | 964 | Isoleucyl-tRNA synthetase, mitochondrial | PRO_0000233335 | |||||
Regions | |||||||||
| Motif | 116 – 126 | 11 | "HIGH" region By similarity | ||||||
| Motif | 664 – 668 | 5 | "KMSKS" region By similarity | ||||||
Sites | |||||||||
| Binding site | 667 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 186 | 1 | Phosphoserine Ref.6 | ||||||
| Modified residue | 189 | 1 | N6-acetyllysine Ref.8 | ||||||
| Modified residue | 233 | 1 | N6-acetyllysine Ref.8 | ||||||
| Modified residue | 241 | 1 | N6-acetyllysine Ref.8 | ||||||
| Modified residue | 775 | 1 | N6-acetyllysine Ref.8 | ||||||
| Modified residue | 781 | 1 | N6-acetyllysine Ref.8 | ||||||
Natural variations | |||||||||
| Natural variant | 14 | 1 | A → V: dbSNP rs2577154. | VAR_059862 | |||||
| Natural variant | 522 | 1 | I → V: dbSNP rs11800305. | VAR_034526 | |||||
Experimental info | |||||||||
| Sequence conflict | 647 | 1 | P → L in BAB14164. Ref.5 | ||||||
| Sequence conflict | 907 | 1 | I → T in BAB14164. Ref.5 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning and characterization of human mitochondrial isoleucine tRNA synthetase gene." Shan Y.X., Guo Z.K., Huang C.Q., Ye G.M., Tang W.W., Yu L. Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. Bentley D.R.Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [3] | "Structure and evolution of two human isoleucyl-tRNA synthetases." Shiba K., Suzuki N., Shigesada K., Schimmel P., Noda T. Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 20-1012. Tissue: Brain. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 56-1012. Tissue: Liver, Placenta and Skin. |
| [5] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 142-1012. |
| [6] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-186, MASS SPECTROMETRY. Tissue: Epithelium. |
| [7] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [8] | "Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-189; LYS-233; LYS-241; LYS-775 AND LYS-781, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AY267462 mRNA. Translation: AAP94033.1. AC103590 Genomic DNA. No translation available. D28500 mRNA. Translation: BAA95147.1. BC010218 mRNA. Translation: AAH10218.2. BC040376 mRNA. Translation: AAH40376.2. BC047880 mRNA. Translation: AAH47880.3. AK001188 mRNA. Translation: BAA91544.1. Frameshift. AK022665 mRNA. Translation: BAB14164.1. Different initiation. | |
| IPI | IPI00017283. |
| RefSeq | NP_060530.3. |
| UniGene | Hs.262823 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1FFY based on UniProtKB P41972. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q9NSE4. 2 interactions. |
| STRING | Q9NSE4. |
PTM databases | |
| PhosphoSite | Q9NSE4. |
Proteomic databases | |
| PRIDE | Q9NSE4. |
Genome annotation databases | |
| Ensembl | ENST00000302637; ENSP00000303279; ENSG00000067704; Homo sapiens. [Genome view] ENST00000366922; ENSP00000355889; ENSG00000067704; Homo sapiens. [Genome view] |
| GeneID | 55699. |
| KEGG | hsa:55699. |
| NMPDR | fig|9606.3.peg.3090. |
| UCSC | uc001hmc.1. human. |
Organism-specific databases | |
| CTD | 55699. |
| GeneCards | GC01P218334. |
| HGNC | HGNC:29685. IARS2. |
| HPA | HPA024212. HPA024594. HPA024596. |
| PharmGKB | PA142671670. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | Q9NSE4. |
| HOVERGEN | Q9NSE4. |
| OMA | FPMRGNL. |
Enzyme and pathway databases | |
| BRENDA | 6.1.1.5. 247. |
| Reactome | REACT_71. Gene Expression. |
Gene expression databases | |
| ArrayExpress | Q9NSE4. |
| Bgee | Q9NSE4. |
| CleanEx | HS_IARS2. |
| Genevestigator | Q9NSE4. |
| GermOnline | ENSG00000067704. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR010663. DNA_glyclase/IsotRNA_synth_Znf. IPR002301. Ile-tRNA-synt_Ia. IPR015905. Ile-tRNA-synt_Ia_N. IPR018353. Isoleucyl-tRNA_synthetase. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view] |
| Gene3D | G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. |
| PANTHER | PTHR11946:SF9. Ile-tRNA-synt_Ia. 1 hit. |
| Pfam | PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF06827. zf-FPG_IleRS. 1 hit. [Graphical view] |
| PRINTS | PR00984. TRNASYNTHILE. |
| TIGRFAMs | TIGR00392. ileS. 1 hit. |
| PROSITE | PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| DrugBank | DB00167. L-Isoleucine. |
| NextBio | 60534. |
Entry information
| Entry name | SYIM_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9NSE4 Secondary accession number(s): Q1M2P9 Q9NW42 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Aminoacyl-tRNA synthetases List of aminoacyl-tRNA synthetase entries |
| Human chromosome 1 Human chromosome 1: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| SIMILARITY comments Index of protein domains and families |

Clusters with


