Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9NSD9 (SYFB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phenylalanine--tRNA ligase beta subunit

EC=6.1.1.20
Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunit
Short name=PheRS
Gene names
Name:FARSB
Synonyms:FARSLB, FRSB
ORF Names:HSPC173
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length589 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).

Subunit structure

Tetramer of two alpha and two beta subunits By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.

Contains 1 B5 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 589589Phenylalanine--tRNA ligase beta subunit
PRO_0000127016

Regions

Domain302 – 37978B5

Experimental info

Sequence conflict2811Q → R in AAF29136. Ref.3
Sequence conflict5551G → V in BAA95608. Ref.1
Sequence conflict5851V → I in BAA95608. Ref.1
Sequence conflict5851V → I in AAD02220. Ref.2
Sequence conflict5851V → I in AAF29136. Ref.3
Sequence conflict5851V → I in AAH17783. Ref.5

Secondary structure

.................................................................................................... 589
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9NSD9 [UniParc].

Last modified May 18, 2010. Version 3.
Checksum: 6425BA46D124BC08

FASTA58966,116
        10         20         30         40         50         60 
MPTVSVKRDL LFQALGRTYT DEEFDELCFE FGLELDEITS EKEIISKEQG NVKAAGASDV 

        70         80         90        100        110        120 
VLYKIDVPAN RYDLLCLEGL VRGLQVFKER IKAPVYKRVM PDGKIQKLII TEETAKIRPF 

       130        140        150        160        170        180 
AVAAVLRNIK FTKDRYDSFI ELQEKLHQNI CRKRALVAIG THDLDTLSGP FTYTAKRPSD 

       190        200        210        220        230        240 
IKFKPLNKTK EYTACELMNI YKTDNHLKHY LHIIENKPLY PVIYDSNGVV LSMPPIINGD 

       250        260        270        280        290        300 
HSRITVNTRN IFIECTGTDF TKAKIVLDII VTMFSEYCEN QFTVEAAEVV FPNGKSHTFP 

       310        320        330        340        350        360 
ELAYRKEMVR ADLINKKVGI RETPENLAKL LTRMYLKSEV IGDGNQIEIE IPPTRADIIH 

       370        380        390        400        410        420 
ACDIVEDAAI AYGYNNIQMT LPKTYTIANQ FPLNKLTELL RHDMAAAGFT EALTFALCSQ 

       430        440        450        460        470        480 
EDIADKLGVD ISATKAVHIS NPKTAEFQVA RTTLLPGLLK TIAANRKMPL PLKLFEISDI 

       490        500        510        520        530        540 
VIKDSNTDVG AKNYRHLCAV YYNKNPGFEI IHGLLDRIMQ LLDVPPGEDK GGYVIKASEG 

       550        560        570        580 
PAFFPGRCAE IFARGQSVGK LGVLHPDVIT KFELTMPCSS LEINVGPFL 

« Hide

References

« Hide 'large scale' references
[1]Motegi H., Noda T., Shiba K.
Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Human phenylalanyl-tRNA synthetase: cloning, characterization of the deduced amino acid sequences in terms of the structural domains and coordinately regulated expression of the alpha and beta subunits in chronic myeloid leukemia cells."
Rodova M., Ankilova V., Safro M.G.
Biochem. Biophys. Res. Commun. 255:765-773(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. expand/collapse author list , Gu J., Chen S.-J., Chen Z.
Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Umbilical cord blood.
[4]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[6]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D84430 mRNA. Translation: BAA95608.1.
AF042346 mRNA. Translation: AAD02220.1.
AF161521 mRNA. Translation: AAF29136.1.
AC097461 Genomic DNA. Translation: AAX88958.1.
AC104772 Genomic DNA. Translation: AAX81986.1.
BC017783 mRNA. Translation: AAH17783.1.
RefSeqNP_005678.3. NM_005687.3.
UniGeneHs.471452.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3L4GX-ray3.30B/D/F/H/J/L/N/P1-589[»]
ProteinModelPortalQ9NSD9.
SMRQ9NSD9. Positions 1-589.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115367. 30 interactions.
DIPDIP-32869N.
IntActQ9NSD9. 7 interactions.
MINTMINT-3073662.
STRING9606.ENSP00000281828.

Chemistry

DrugBankDB00120. L-Phenylalanine.

PTM databases

PhosphoSiteQ9NSD9.

Polymorphism databases

DMDM296452943.

Proteomic databases

PaxDbQ9NSD9.
PRIDEQ9NSD9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000281828; ENSP00000281828; ENSG00000116120.
GeneID10056.
KEGGhsa:10056.
UCSCuc002vne.1. human.

Organism-specific databases

CTD10056.
GeneCardsGC02M223435.
H-InvDBHIX0002878.
HGNCHGNC:17800. FARSB.
HPAHPA036678.
MIM609690. gene.
neXtProtNX_Q9NSD9.
PharmGKBPA162388068.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0072.
HOGENOMHOG000105095.
HOVERGENHBG009523.
InParanoidQ9NSD9.
KOK01890.
OMASEYCENQ.
OrthoDBEOG7R2BJ6.
PhylomeDBQ9NSD9.
TreeFamTF105681.

Enzyme and pathway databases

BRENDA6.1.1.20. 2681.
ReactomeREACT_71. Gene Expression.

Gene expression databases

ArrayExpressQ9NSD9.
BgeeQ9NSD9.
CleanExHS_FARSB.
GenevestigatorQ9NSD9.

Family and domain databases

Gene3D3.30.56.20. 1 hit.
InterProIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR004531. Phe-tRNA-synth_IIc_bsu_arc.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
[Graphical view]
SMARTSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
[Graphical view]
SUPFAMSSF46955. SSF46955. 2 hits.
SSF56037. SSF56037. 1 hit.
TIGRFAMsTIGR00471. pheT_arch. 1 hit.
PROSITEPS51483. B5. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSFARSB. human.
GeneWikiFARSB.
GenomeRNAi10056.
NextBio37993.
PROQ9NSD9.
SOURCESearch...

Entry information

Entry nameSYFB_HUMAN
AccessionPrimary (citable) accession number: Q9NSD9
Secondary accession number(s): O95708 expand/collapse secondary AC list , Q4ZFX1, Q57ZJ5, Q9NZZ6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: May 18, 2010
Last modified: April 16, 2014
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries