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Protein

Phenylalanine--tRNA ligase beta subunit

Gene

FARSB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).1 Publication

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • magnesium ion binding Source: InterPro
  • phenylalanine-tRNA ligase activity Source: UniProtKB
  • RNA binding Source: InterPro

GO - Biological processi

  • phenylalanyl-tRNA aminoacylation Source: UniProtKB
  • protein heterotetramerization Source: UniProtKB
  • translation Source: ProtInc
  • tRNA aminoacylation for protein translation Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS03982-MONOMER.
BRENDAi6.1.1.20. 2681.
ReactomeiR-HSA-379716. Cytosolic tRNA aminoacylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase beta subunit (EC:6.1.1.201 Publication)
Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunit
Short name:
PheRS
Gene namesi
Name:FARSB
Synonyms:FARSLB, FRSB
ORF Names:HSPC173
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:17800. FARSB.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: ProtInc
  • cytosol Source: Reactome
  • membrane Source: UniProtKB
  • phenylalanine-tRNA ligase complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi10056.
OpenTargetsiENSG00000116120.
PharmGKBiPA162388068.

Chemistry databases

DrugBankiDB00120. L-Phenylalanine.

Polymorphism and mutation databases

BioMutaiFARSB.
DMDMi296452943.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001270161 – 589Phenylalanine--tRNA ligase beta subunitAdd BLAST589

Proteomic databases

EPDiQ9NSD9.
MaxQBiQ9NSD9.
PaxDbiQ9NSD9.
PeptideAtlasiQ9NSD9.
PRIDEiQ9NSD9.

PTM databases

iPTMnetiQ9NSD9.
PhosphoSitePlusiQ9NSD9.
SwissPalmiQ9NSD9.

Expressioni

Gene expression databases

BgeeiENSG00000116120.
CleanExiHS_FARSB.
GenevisibleiQ9NSD9. HS.

Organism-specific databases

HPAiHPA036677.
HPA036678.
HPA061398.

Interactioni

Subunit structurei

Heterotetramer; dimer of two heterodimers formed by FARSA and FARSB.1 Publication

Protein-protein interaction databases

BioGridi115367. 45 interactors.
DIPiDIP-32869N.
IntActiQ9NSD9. 11 interactors.
MINTiMINT-3073662.
STRINGi9606.ENSP00000281828.

Structurei

Secondary structure

1589
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 7Combined sources5
Helixi8 – 15Combined sources8
Helixi23 – 30Combined sources8
Beta strandi34 – 40Combined sources7
Helixi41 – 45Combined sources5
Beta strandi48 – 50Combined sources3
Beta strandi56 – 66Combined sources11
Helixi77 – 87Combined sources11
Beta strandi97 – 99Combined sources3
Beta strandi107 – 110Combined sources4
Turni112 – 117Combined sources6
Beta strandi120 – 126Combined sources7
Helixi133 – 145Combined sources13
Turni146 – 155Combined sources10
Beta strandi156 – 163Combined sources8
Helixi164 – 166Combined sources3
Beta strandi171 – 176Combined sources6
Beta strandi178 – 180Combined sources3
Helixi194 – 200Combined sources7
Turni205 – 214Combined sources10
Beta strandi220 – 224Combined sources5
Turni234 – 236Combined sources3
Beta strandi237 – 240Combined sources4
Beta strandi251 – 258Combined sources8
Helixi260 – 274Combined sources15
Helixi275 – 277Combined sources3
Beta strandi281 – 285Combined sources5
Beta strandi287 – 290Combined sources4
Beta strandi296 – 299Combined sources4
Beta strandi305 – 310Combined sources6
Helixi311 – 318Combined sources8
Helixi324 – 333Combined sources10
Beta strandi337 – 340Combined sources4
Beta strandi342 – 351Combined sources10
Helixi361 – 372Combined sources12
Helixi374 – 376Combined sources3
Helixi392 – 406Combined sources15
Beta strandi416 – 418Combined sources3
Helixi420 – 423Combined sources4
Helixi425 – 427Combined sources3
Beta strandi438 – 441Combined sources4
Helixi445 – 447Combined sources3
Beta strandi448 – 450Combined sources3
Helixi455 – 464Combined sources10
Turni465 – 467Combined sources3
Beta strandi472 – 483Combined sources12
Beta strandi490 – 505Combined sources16
Helixi508 – 521Combined sources14
Turni528 – 531Combined sources4
Beta strandi532 – 537Combined sources6
Beta strandi543 – 553Combined sources11
Beta strandi556 – 564Combined sources9
Helixi566 – 571Combined sources6
Beta strandi578 – 584Combined sources7
Helixi586 – 588Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3L4GX-ray3.30B/D/F/H/J/L/N/P1-589[»]
ProteinModelPortaliQ9NSD9.
SMRiQ9NSD9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini302 – 379B5PROSITE-ProRule annotationAdd BLAST78

Sequence similaritiesi

Contains 1 B5 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2472. Eukaryota.
COG0072. LUCA.
GeneTreeiENSGT00530000063489.
HOGENOMiHOG000105095.
HOVERGENiHBG009523.
InParanoidiQ9NSD9.
KOiK01890.
OMAiERPYVTG.
OrthoDBiEOG091G04GW.
PhylomeDBiQ9NSD9.
TreeFamiTF105681.

Family and domain databases

Gene3Di3.30.56.20. 1 hit.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR004531. Phe-tRNA-synth_IIc_bsu_arc.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 2 hits.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00471. pheT_arch. 1 hit.
PROSITEiPS51483. B5. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NSD9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPTVSVKRDL LFQALGRTYT DEEFDELCFE FGLELDEITS EKEIISKEQG
60 70 80 90 100
NVKAAGASDV VLYKIDVPAN RYDLLCLEGL VRGLQVFKER IKAPVYKRVM
110 120 130 140 150
PDGKIQKLII TEETAKIRPF AVAAVLRNIK FTKDRYDSFI ELQEKLHQNI
160 170 180 190 200
CRKRALVAIG THDLDTLSGP FTYTAKRPSD IKFKPLNKTK EYTACELMNI
210 220 230 240 250
YKTDNHLKHY LHIIENKPLY PVIYDSNGVV LSMPPIINGD HSRITVNTRN
260 270 280 290 300
IFIECTGTDF TKAKIVLDII VTMFSEYCEN QFTVEAAEVV FPNGKSHTFP
310 320 330 340 350
ELAYRKEMVR ADLINKKVGI RETPENLAKL LTRMYLKSEV IGDGNQIEIE
360 370 380 390 400
IPPTRADIIH ACDIVEDAAI AYGYNNIQMT LPKTYTIANQ FPLNKLTELL
410 420 430 440 450
RHDMAAAGFT EALTFALCSQ EDIADKLGVD ISATKAVHIS NPKTAEFQVA
460 470 480 490 500
RTTLLPGLLK TIAANRKMPL PLKLFEISDI VIKDSNTDVG AKNYRHLCAV
510 520 530 540 550
YYNKNPGFEI IHGLLDRIMQ LLDVPPGEDK GGYVIKASEG PAFFPGRCAE
560 570 580
IFARGQSVGK LGVLHPDVIT KFELTMPCSS LEINVGPFL
Length:589
Mass (Da):66,116
Last modified:May 18, 2010 - v3
Checksum:i6425BA46D124BC08
GO
Isoform 2 (identifier: Q9NSD9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-99: Missing.

Note: No experimental confirmation available.
Show »
Length:490
Mass (Da):54,861
Checksum:iEB9B48047A2AF094
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti281Q → R in AAF29136 (PubMed:11042152).Curated1
Sequence conflicti555G → V in BAA95608 (Ref. 1) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_071245585V → I.6 PublicationsCorresponds to variant rs7185dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0568661 – 99Missing in isoform 2. 1 PublicationAdd BLAST99

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84430 mRNA. Translation: BAA95608.1.
AF042346 mRNA. Translation: AAD02220.1.
AF161521 mRNA. Translation: AAF29136.1.
AK294158 mRNA. Translation: BAG57481.1.
AC097461 Genomic DNA. Translation: AAX88958.1.
AC104772 Genomic DNA. Translation: AAX81986.1.
CH471063 Genomic DNA. Translation: EAW70804.1.
BC017783 mRNA. Translation: AAH17783.1.
CCDSiCCDS2454.1. [Q9NSD9-1]
RefSeqiNP_005678.3. NM_005687.4. [Q9NSD9-1]
XP_006712232.1. XM_006712169.2. [Q9NSD9-2]
XP_011508768.1. XM_011510466.2. [Q9NSD9-2]
XP_016858599.1. XM_017003110.1. [Q9NSD9-2]
UniGeneiHs.471452.

Genome annotation databases

EnsembliENST00000281828; ENSP00000281828; ENSG00000116120. [Q9NSD9-1]
GeneIDi10056.
KEGGihsa:10056.
UCSCiuc002vne.2. human. [Q9NSD9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84430 mRNA. Translation: BAA95608.1.
AF042346 mRNA. Translation: AAD02220.1.
AF161521 mRNA. Translation: AAF29136.1.
AK294158 mRNA. Translation: BAG57481.1.
AC097461 Genomic DNA. Translation: AAX88958.1.
AC104772 Genomic DNA. Translation: AAX81986.1.
CH471063 Genomic DNA. Translation: EAW70804.1.
BC017783 mRNA. Translation: AAH17783.1.
CCDSiCCDS2454.1. [Q9NSD9-1]
RefSeqiNP_005678.3. NM_005687.4. [Q9NSD9-1]
XP_006712232.1. XM_006712169.2. [Q9NSD9-2]
XP_011508768.1. XM_011510466.2. [Q9NSD9-2]
XP_016858599.1. XM_017003110.1. [Q9NSD9-2]
UniGeneiHs.471452.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3L4GX-ray3.30B/D/F/H/J/L/N/P1-589[»]
ProteinModelPortaliQ9NSD9.
SMRiQ9NSD9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115367. 45 interactors.
DIPiDIP-32869N.
IntActiQ9NSD9. 11 interactors.
MINTiMINT-3073662.
STRINGi9606.ENSP00000281828.

Chemistry databases

DrugBankiDB00120. L-Phenylalanine.

PTM databases

iPTMnetiQ9NSD9.
PhosphoSitePlusiQ9NSD9.
SwissPalmiQ9NSD9.

Polymorphism and mutation databases

BioMutaiFARSB.
DMDMi296452943.

Proteomic databases

EPDiQ9NSD9.
MaxQBiQ9NSD9.
PaxDbiQ9NSD9.
PeptideAtlasiQ9NSD9.
PRIDEiQ9NSD9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000281828; ENSP00000281828; ENSG00000116120. [Q9NSD9-1]
GeneIDi10056.
KEGGihsa:10056.
UCSCiuc002vne.2. human. [Q9NSD9-1]

Organism-specific databases

CTDi10056.
DisGeNETi10056.
GeneCardsiFARSB.
H-InvDBHIX0002878.
HGNCiHGNC:17800. FARSB.
HPAiHPA036677.
HPA036678.
HPA061398.
MIMi609690. gene.
neXtProtiNX_Q9NSD9.
OpenTargetsiENSG00000116120.
PharmGKBiPA162388068.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2472. Eukaryota.
COG0072. LUCA.
GeneTreeiENSGT00530000063489.
HOGENOMiHOG000105095.
HOVERGENiHBG009523.
InParanoidiQ9NSD9.
KOiK01890.
OMAiERPYVTG.
OrthoDBiEOG091G04GW.
PhylomeDBiQ9NSD9.
TreeFamiTF105681.

Enzyme and pathway databases

BioCyciZFISH:HS03982-MONOMER.
BRENDAi6.1.1.20. 2681.
ReactomeiR-HSA-379716. Cytosolic tRNA aminoacylation.

Miscellaneous databases

ChiTaRSiFARSB. human.
GeneWikiiFARSB.
GenomeRNAii10056.
PROiQ9NSD9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000116120.
CleanExiHS_FARSB.
GenevisibleiQ9NSD9. HS.

Family and domain databases

Gene3Di3.30.56.20. 1 hit.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR004531. Phe-tRNA-synth_IIc_bsu_arc.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 2 hits.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00471. pheT_arch. 1 hit.
PROSITEiPS51483. B5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYFB_HUMAN
AccessioniPrimary (citable) accession number: Q9NSD9
Secondary accession number(s): B4DFM0
, O95708, Q4ZFX1, Q57ZJ5, Q9NZZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: May 18, 2010
Last modified: November 2, 2016
This is version 157 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.