Q9NSC5 (HOME3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homer protein homolog 3
Short name=Homer-3

Gene names

Name:

HOMER3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses.
Subunit structure	Isoform 1 and isoform 2 encode coiled-coil structures that mediate homo- and heteromultimerization.
Subcellular location	Cytoplasm By similarity. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density By similarity. Cell junction › synapse By similarity. Note: Postsynaptic density of neuronal cells By similarity.
Domain	The WH1 domain interacts with the PPXXF motif in GRM1, GRM5, RYR1, RYR2, ITPR1, SHANK 1 and SHANK3.
Sequence similarities	Belongs to the Homer family. Contains 1 WH1 domain.
Sequence caution	The sequence AAB81545.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Cytoplasm Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled glutamate receptor signaling pathway Traceable author statement Ref.1. Source: ProtInc protein targeting Traceable author statement Ref.2. Source: UniProtKB
Cellular_component	basal part of cell Inferred from electronic annotation. Source: Compara cell junction Inferred from electronic annotation. Source: UniProtKB-KW cytosol Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW postsynaptic density Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
APP	P05067-8	3	EBI-748420,EBI-302661
PAX6	P26367	3	EBI-748420,EBI-747278

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NSC5-1)

Also known as: 3a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NSC5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     299-301: Missing.

Isoform 3 (identifier: Q9NSC5-3)

Also known as: 3c;

The sequence of this isoform differs from the canonical sequence as follows:
     124-145: GELTSPALGLASHQVPPSPLVS → WGGPQSALVVGSFGAVFELLIV
     146-361: Missing.

Isoform 4 (identifier: Q9NSC5-4)

Also known as: 3d;

The sequence of this isoform differs from the canonical sequence as follows:
     118-121: EKSQ → QLQR
     122-361: Missing.

Isoform 5 (identifier: Q9NSC5-5)

The sequence of this isoform differs from the canonical sequence as follows:
     101-136: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 361

361

Homer protein homolog 3

PRO_0000191011

Regions

Domain

1 – 113

113

WH1

Coiled coil

191 – 243

53

Potential

Coiled coil

254 – 358

105

Potential

Compositional bias

206 – 211

6

Poly-Ala

Amino acid modifications

Modified residue

159

1

Phosphoserine Ref.5

Natural variations

Alternative sequence

101 – 136

36

Missing in isoform 5.

VSP_045715

Alternative sequence

118 – 121

4

EKSQ → QLQR in isoform 4.

VSP_009073

Alternative sequence

122 – 361

240

Missing in isoform 4.

VSP_009074

Alternative sequence

124 – 145

22

GELTS…SPLVS → WGGPQSALVVGSFGAVFELL IV in isoform 3.

VSP_009075

Alternative sequence

146 – 361

216

Missing in isoform 3.

VSP_009076

Alternative sequence

299 – 301

3

Missing in isoform 2.

VSP_009077

Natural variant

342

1

S → R. Ref.2 Ref.4
Corresponds to variant rs1059240 [ dbSNP | Ensembl ].

VAR_017410

Secondary structure

1

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361

Helix Strand Turn

Details...

Beta strand

5 – 19

15

Turn

21 – 23

3

Beta strand

26 – 33

8

Beta strand

35 – 42

8

Turn

43 – 46

4

Beta strand

47 – 54

8

Beta strand

57 – 63

7

Beta strand

70 – 72

3

Beta strand

74 – 82

9

Turn

83 – 86

4

Beta strand

87 – 92

6

Helix

96 – 117

22

Helix

295 – 358

64

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (3a) [UniParc]. Last modified November 28, 2003. Version 2. Checksum: 9FCC62319FF5165A	FASTA	361	39,836
	10 20 30 40 50 60 MSTAREQPIF STRAHVFQID PATKRNWIPA GKHALTVSYF YDATRNVYRI ISIGGAKAII 70 80 90 100 110 120 NSTVTPNMTF TKTSQKFGQW ADSRANTVYG LGFASEQHLT QFAEKFQEVK EAARLAREKS 130 140 150 160 170 180 QDGGELTSPA LGLASHQVPP SPLVSANGPG EEKLFRSQSA DAPGPTERER LKKMLSEGSV 190 200 210 220 230 240 GEVQWEAEFF ALQDSNNKLA GALREANAAA AQWRQQLEAQ RAEAERLRQR VAELEAQAAS 250 260 270 280 290 300 EVTPTGEKEG LGQGQSLEQL EALVQTKDQE IQTLKSQTGG PREALEAAER EETQQKVQDL 310 320 330 340 350 360 ETRNAELEHQ LRAMERSLEE ARAERERARA EVGRAAQLLD VSLFELSELR EGLARLAEAA P
	Isoform 2 [UniParc]. Checksum: 0799594DEABE399F	FASTA	358	39,479
	10 20 30 40 50 60 MSTAREQPIF STRAHVFQID PATKRNWIPA GKHALTVSYF YDATRNVYRI ISIGGAKAII 70 80 90 100 110 120 NSTVTPNMTF TKTSQKFGQW ADSRANTVYG LGFASEQHLT QFAEKFQEVK EAARLAREKS 130 140 150 160 170 180 QDGGELTSPA LGLASHQVPP SPLVSANGPG EEKLFRSQSA DAPGPTERER LKKMLSEGSV 190 200 210 220 230 240 GEVQWEAEFF ALQDSNNKLA GALREANAAA AQWRQQLEAQ RAEAERLRQR VAELEAQAAS 250 260 270 280 290 300 EVTPTGEKEG LGQGQSLEQL EALVQTKDQE IQTLKSQTGG PREALEAAER EETQQKVQTR 310 320 330 340 350 NAELEHQLRA MERSLEEARA ERERARAEVG RAAQLLDVSL FELSELREGL ARLAEAAP
	Isoform 3 (3c) [UniParc]. Checksum: 4DA4F12F2893744C	FASTA	145	16,032
	10 20 30 40 50 60 MSTAREQPIF STRAHVFQID PATKRNWIPA GKHALTVSYF YDATRNVYRI ISIGGAKAII 70 80 90 100 110 120 NSTVTPNMTF TKTSQKFGQW ADSRANTVYG LGFASEQHLT QFAEKFQEVK EAARLAREKS 130 140 QDGWGGPQSA LVVGSFGAVF ELLIV
	Isoform 4 (3d) [UniParc]. Checksum: 42C572EB13211534	FASTA	121	13,685
	10 20 30 40 50 60 MSTAREQPIF STRAHVFQID PATKRNWIPA GKHALTVSYF YDATRNVYRI ISIGGAKAII 70 80 90 100 110 120 NSTVTPNMTF TKTSQKFGQW ADSRANTVYG LGFASEQHLT QFAEKFQEVK EAARLARQLQ R
	Isoform 5 [UniParc]. Checksum: F51CA5A05CB92734	FASTA	325	35,954
	10 20 30 40 50 60 MSTAREQPIF STRAHVFQID PATKRNWIPA GKHALTVSYF YDATRNVYRI ISIGGAKAII 70 80 90 100 110 120 NSTVTPNMTF TKTSQKFGQW ADSRANTVYG LGFASEQHLT QVPPSPLVSA NGPGEEKLFR 130 140 150 160 170 180 SQSADAPGPT ERERLKKMLS EGSVGEVQWE AEFFALQDSN NKLAGALREA NAAAAQWRQQ 190 200 210 220 230 240 LEAQRAEAER LRQRVAELEA QAASEVTPTG EKEGLGQGQS LEQLEALVQT KDQEIQTLKS 250 260 270 280 290 300 QTGGPREALE AAEREETQQK VQDLETRNAE LEHQLRAMER SLEEARAERE RARAEVGRAA 310 320 QLLDVSLFEL SELREGLARL AEAAP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Homer regulates the association of group 1 metabotropic glutamate receptors with multivalent complexes of homer-related, synaptic proteins." Xiao B., Tu J.C., Petralia R.S., Yuan J.P., Doan A., Breder C.D., Ruggiero A., Lanahan A.A., Wenthold R.J., Worley P.F. Neuron 21:707-716(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Brain.
[2]	"Molecular characterisation of two structurally distinct groups of human homers, generated by extensive alternative splicing." Soloviev M., Ciruela F., Chan W.-Y., McIlhinney R.A.J. J. Mol. Biol. 295:1185-1200(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 3; 4 AND 5), VARIANT ARG-342.
[3]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ARG-342. Tissue: Uterus.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-159, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF093265 mRNA. Translation: AAC71029.1.
Y17573 mRNA. Translation: CAB75536.1.
Y18894 mRNA. Translation: CAB75543.1.
Y18895 mRNA. Translation: CAB75544.1.
Y18896 mRNA. Translation: CAB75545.1.
AC002985 Genomic DNA. Translation: AAB81545.1. Sequence problems.
BC012113 mRNA. Translation: AAH12113.1.

IPI

IPI00160395.
IPI00395002.
IPI00395003.
IPI00395005.
IPI00396543.

RefSeq

NP_001139193.1. NM_001145721.1.
NP_001139194.1. NM_001145722.1.
NP_001139196.1. NM_001145724.1.
NP_004829.3. NM_004838.3.

UniGene

Hs.720208.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2P8V	X-ray	1.85	A	2-118	[»]
3CVF	X-ray	2.90	A/B/C/D	287-361	[»]

ProteinModelPortal

Q9NSC5.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9NSC5. 16 interactions.

MINT

MINT-3370287.

STRING

9606.ENSP00000348150.

PTM databases

PhosphoSite

Q9NSC5.

Polymorphism databases

DMDM

38605068.

Proteomic databases

PaxDb

Q9NSC5.

PRIDE

Q9NSC5.

Protocols and materials databases

DNASU

9454.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000221222; ENSP00000221222; ENSG00000051128.
ENST00000355887; ENSP00000348150; ENSG00000051128.
ENST00000392351; ENSP00000376162; ENSG00000051128.
ENST00000433218; ENSP00000396154; ENSG00000051128.
ENST00000539827; ENSP00000439937; ENSG00000051128.
ENST00000542541; ENSP00000446026; ENSG00000051128.
ENST00000594439; ENSP00000471835; ENSG00000051128.

GeneID

9454.

KEGG

hsa:9454.

UCSC

uc002nku.2. human.
uc002nkw.2. human.

Organism-specific databases

CTD

9454.

GeneCards

GC19M019040.

HGNC

HGNC:17514. HOMER3.

HPA

CAB032656.

MIM

604800. gene.

neXtProt

NX_Q9NSC5.

PharmGKB

PA134988320.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG76046.

HOGENOM

HOG000006979.

HOVERGEN

HBG051918.

InParanoid

Q9NSC5.

KO

K15010.

OMA

AEREETQ.

PhylomeDB

Q9NSC5.

Gene expression databases

ArrayExpress

Q9NSC5.

Bgee

Q9NSC5.

CleanEx

HS_HOMER3.

Genevestigator

Q9NSC5.

GermOnline

ENSG00000051128. Homo sapiens.

Family and domain databases

Gene3D

2.30.29.30. 1 hit.

InterPro

IPR000697. EVH1.
IPR011993. PH_like_dom.
[Graphical view]

Pfam

PF00568. WH1. 1 hit.
[Graphical view]

SMART

SM00461. WH1. 1 hit.
[Graphical view]

PROSITE

PS50229. WH1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9NSC5.

GenomeRNAi

9454.

NextBio

35420.

SOURCE

Search...

Entry information

Entry name

HOME3_HUMAN

Accession

Primary (citable) accession number: Q9NSC5
Secondary accession number(s): E9PCW9 , O14580, O95350, Q9NSB9, Q9NSC0, Q9NSC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	November 28, 2003
Last modified:	May 1, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.