Q9NSB8 (HOME2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homer protein homolog 2
Short name=Homer-2

Alternative name(s):
Cupidin

Gene names

Name:

HOMER2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses.
Subunit structure	Isoform 1 and isoform 2 encode coiled-coil structures that mediate homo- and heteromultimerization.
Subcellular location	Cytoplasm. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Cell junction › synapse. Note: Postsynaptic density of neuronal cells. The stabilization and clustering of the metabotropic glutamate receptors appears to be mediated by isoform 1 and isoform 2 at the cell surface.
Domain	The WH1 domain interacts with the PPXXF motif in GRM1, GRM5, RYR1, RYR2, ITPR1, SHANK 1 and SHANK3.
Sequence similarities	Belongs to the Homer family. Contains 1 WH1 domain.
Sequence caution	The sequence CAB75539.1 differs from that shown. Reason: Aberrant splicing. The sequence CAB75540.1 differs from that shown. Reason: Aberrant splicing.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Cytoplasm Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled glutamate receptor signaling pathway Traceable author statement Ref.1. Source: ProtInc behavioral response to cocaine Inferred from electronic annotation. Source: Compara calcium-mediated signaling using intracellular calcium source Inferred from electronic annotation. Source: Compara chemical homeostasis within a tissue Inferred from electronic annotation. Source: Compara regulation of G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: Compara
Cellular_component	apical part of cell Inferred from electronic annotation. Source: Compara cell junction Inferred from electronic annotation. Source: UniProtKB-KW cytoplasm Inferred from direct assay. Source: HPA neuronal cell body Inferred from electronic annotation. Source: Compara plasma membrane Inferred from direct assay. Source: HPA postsynaptic density Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NSB8-1)

Also known as: 2b;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NSB8-2)

Also known as: 2a;

The sequence of this isoform differs from the canonical sequence as follows:
     130-140: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Homer protein homolog 2		PRO_0000191008
Regions
	Domain	1 – 110	110	WH1
	Coiled coil	92 – 122	31	Potential
	Coiled coil	160 – 329	170	Potential
Natural variations
	Alternative sequence	130 – 140	11	Missing in isoform 2.		VSP_041731
	Natural variant	219	1	R → H. Corresponds to variant rs7175005 [ dbSNP | Ensembl ].		VAR_053366
	Natural variant	239	1	I → S. Corresponds to variant rs17158223 [ dbSNP | Ensembl ].		VAR_053367
Experimental info
	Sequence conflict	149	1	D → E in AAC71027. Ref.1
	Sequence conflict	149	1	D → E in AAC71028. Ref.1
	Sequence conflict	161	1	H → Q in AAC71027. Ref.1
	Sequence conflict	161	1	H → Q in AAC71028. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (2b) [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 33D258177F798373	FASTA	354	40,627
	10 20 30 40 50 60 MGEQPIFTTR AHVFQIDPNT KKNWMPASKQ AVTVSYFYDV TRNSYRIISV DGAKVIINST 70 80 90 100 110 120 ITPNMTFTKT SQKFGQWADS RANTVFGLGF SSEQQLTKFA EKFQEVKEAA KIAKDKTQEK 130 140 150 160 170 180 IETSSNHSQE SGRETPSSTQ ASSVNGTDDE KASHAGPANT HLKSENDKLK IALTQSAANV 190 200 210 220 230 240 KKWEIELQTL RESNARLTTA LQESAASVEQ WKRQFSICRD ENDRLRNKID ELEEQCSEIN 250 260 270 280 290 300 REKEKNTQLK RRIEELEAEL REKETELKDL RKQSEIIPQL MSECEYVSEK LEAAERDNQN 310 320 330 340 350 LEDKVRSLKT DIEESKYRQR HLKVELKSFL EVLDGKIDDL HDFRRGLSKL GTDN
	Isoform 2 (2a) [UniParc]. Checksum: 2980EBF0280F1772	FASTA	343	39,467
	10 20 30 40 50 60 MGEQPIFTTR AHVFQIDPNT KKNWMPASKQ AVTVSYFYDV TRNSYRIISV DGAKVIINST 70 80 90 100 110 120 ITPNMTFTKT SQKFGQWADS RANTVFGLGF SSEQQLTKFA EKFQEVKEAA KIAKDKTQEK 130 140 150 160 170 180 IETSSNHSQA SSVNGTDDEK ASHAGPANTH LKSENDKLKI ALTQSAANVK KWEIELQTLR 190 200 210 220 230 240 ESNARLTTAL QESAASVEQW KRQFSICRDE NDRLRNKIDE LEEQCSEINR EKEKNTQLKR 250 260 270 280 290 300 RIEELEAELR EKETELKDLR KQSEIIPQLM SECEYVSEKL EAAERDNQNL EDKVRSLKTD 310 320 330 340 IEESKYRQRH LKVELKSFLE VLDGKIDDLH DFRRGLSKLG TDN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Homer regulates the association of group 1 metabotropic glutamate receptors with multivalent complexes of homer-related, synaptic proteins." Xiao B., Tu J.C., Petralia R.S., Yuan J.P., Doan A., Breder C.D., Ruggiero A., Lanahan A.A., Wenthold R.J., Worley P.F. Neuron 21:707-716(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Frontal cortex.
[2]	"Molecular characterisation of two structurally distinct groups of human homers, generated by extensive alternative splicing." Soloviev M., Ciruela F., Chan W.-Y., McIlhinney R.A.J. J. Mol. Biol. 295:1185-1200(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), NUCLEOTIDE SEQUENCE [MRNA] OF 1-182 (ISOFORMS 1/2).
[3]	"Mammalian neuralized interacts with a novel protein which has a EVH1-like domain." Yoshida M., Ueno M., Nakamura H., Saya H. Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Brain.
[4]	"Analysis of the DNA sequence and duplication history of human chromosome 15." Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C. Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Lung.
[6]	"Homer binds a novel proline-rich motif and links group 1 metabotropic glutamate receptors with IP3 receptors." Tu J.C., Xiao B., Yuan J.P., Lanahan A.A., Leoffert K., Li M., Linden D.J., Worley P.F. Neuron 21:717-726(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GRM1; GRM5; DYN3 AND ITPR1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF093263 mRNA. Translation: AAC71027.1. AF093264 mRNA. Translation: AAC71028.1. Y19025 mRNA. Translation: CAB75537.1. Y19026 mRNA. Translation: CAB75538.1. Y19027 mRNA. Translation: CAB75539.1. Sequence problems. Y19028 mRNA. Translation: CAB75540.1. Sequence problems. AF081530 mRNA. Translation: AAD13748.1. AC022558 Genomic DNA. No translation available. AC044907 Genomic DNA. No translation available. BC012109 mRNA. Translation: AAH12109.1.
IPI	IPI00394745. IPI00394746.
RefSeq	NP_004830.2. NM_004839.3. NP_955362.1. NM_199330.2.
UniGene	Hs.578443.
3D structure databases
ProteinModelPortal	Q9NSB8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NSB8. 2 interactions.
STRING	9606.ENSP00000305632.
PTM databases
PhosphoSite	Q9NSB8.
Polymorphism databases
DMDM	38605067.
Proteomic databases
PaxDb	Q9NSB8.
PRIDE	Q9NSB8.
Protocols and materials databases
DNASU	9455.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000304231; ENSP00000305632; ENSG00000103942. ENST00000450735; ENSP00000407634; ENSG00000103942.
GeneID	9455.
KEGG	hsa:9455.
UCSC	uc002bjg.3. human. uc002bjh.3. human.
Organism-specific databases
CTD	9455.
GeneCards	GC15M083517.
H-InvDB	HIX0037900.
HGNC	HGNC:17513. HOMER2.
HPA	HPA040134.
MIM	604799. gene.
neXtProt	NX_Q9NSB8.
PharmGKB	PA134870500.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG68331.
HOGENOM	HOG000006979.
HOVERGEN	HBG051918.
InParanoid	Q9NSB8.
KO	K15010.
OMA	NWMPASK.
PhylomeDB	Q9NSB8.
Gene expression databases
ArrayExpress	Q9NSB8.
Bgee	Q9NSB8.
CleanEx	HS_HOMER2.
Genevestigator	Q9NSB8.
GermOnline	ENSG00000103942. Homo sapiens.
Family and domain databases
Gene3D	2.30.29.30. 1 hit.
InterPro	IPR000697. EVH1. IPR011993. PH_like_dom. [Graphical view]
Pfam	PF00568. WH1. 1 hit. [Graphical view]
SMART	SM00461. WH1. 1 hit. [Graphical view]
PROSITE	PS50229. WH1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	9455.
NextBio	35424.
SOURCE	Search...

Entry information

Entry name

HOME2_HUMAN

Accession

Primary (citable) accession number: Q9NSB8
Secondary accession number(s): O95269 , O95349, Q9NSB6, Q9NSB7, Q9UNT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.