Reviewed, UniProtKB/Swiss-Prot Q9NSB8 (HOME2_HUMAN)

Names and origin

Protein names

Recommended name:
    Homer protein homolog 2
      Short name=Homer-2
Alternative name(s):
    Cupidin

Gene names

Name:

HOMER2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses.
Subunit structure	Isoform 1 and isoform 2 encode coiled-coil structures that mediate homo- and heteromultimerization.
Subcellular location	Cytoplasm. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Cell junction › synapse. Note: Postsynaptic density of neuronal cells. The stabilization and clustering of the metabotropic glutamate receptors appears to be mediated by isoform 1 and isoform 2 at the cell surface.
Domain	The WH1 domain interacts with the PPXXF motif in GRM1, GRM5, RYR1, RYR2, ITPR1, SHANK 1 and SHANK3.
Sequence similarities	Belongs to the Homer family. Contains 1 WH1 domain.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Cytoplasm Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
Gene Ontology (GO)
Biological process	metabotropic glutamate receptor signaling pathway Ref.1 Traceable author statement. Source: ProtInc
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic density Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
APP	P05067-8	1	EBI-2126733,EBI-302661

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NSB8-1)

Also known as: 2b;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NSB8-2)

Also known as: 2a;

The sequence of this isoform differs from the canonical sequence as follows:
     130-140: Missing.

Isoform 3 (identifier: Q9NSB8-3)

Also known as: 2d;

The sequence of this isoform differs from the canonical sequence as follows:
     183-354: Missing.

Isoform 4 (identifier: Q9NSB8-4)

Also known as: 2c;

The sequence of this isoform differs from the canonical sequence as follows:
     130-140: Missing.
     183-354: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Homer protein homolog 2		PRO_0000191008
Regions
	Domain	1 – 110	110	WH1
	Coiled coil	92 – 122	31	Potential
	Coiled coil	160 – 329	170	Potential
Natural variations
	Alternative sequence	130 – 140	11	Missing in isoform 2 and isoform 4.		VSP_009069
	Alternative sequence	183 – 354	172	Missing in isoform 3 and isoform 4.		VSP_009070
	Natural variant	219	1	R → H: dbSNP rs7175005.		VAR_053366
	Natural variant	239	1	I → S: dbSNP rs17158223.		VAR_053367
Experimental info
	Sequence conflict	149	1	D → E in AAC71028. Ref.1
	Sequence conflict	161	1	H → Q in AAC71028. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (2b) [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 33D258177F798373	FASTA	354	40,627
	10 20 30 40 50 60 MGEQPIFTTR AHVFQIDPNT KKNWMPASKQ AVTVSYFYDV TRNSYRIISV DGAKVIINST 70 80 90 100 110 120 ITPNMTFTKT SQKFGQWADS RANTVFGLGF SSEQQLTKFA EKFQEVKEAA KIAKDKTQEK 130 140 150 160 170 180 IETSSNHSQE SGRETPSSTQ ASSVNGTDDE KASHAGPANT HLKSENDKLK IALTQSAANV 190 200 210 220 230 240 KKWEIELQTL RESNARLTTA LQESAASVEQ WKRQFSICRD ENDRLRNKID ELEEQCSEIN 250 260 270 280 290 300 REKEKNTQLK RRIEELEAEL REKETELKDL RKQSEIIPQL MSECEYVSEK LEAAERDNQN 310 320 330 340 350 LEDKVRSLKT DIEESKYRQR HLKVELKSFL EVLDGKIDDL HDFRRGLSKL GTDN
	Isoform 2 (2a). Checksum: 2980EBF0280F1772	FASTA	343	39,467
	10 20 30 40 50 60 MGEQPIFTTR AHVFQIDPNT KKNWMPASKQ AVTVSYFYDV TRNSYRIISV DGAKVIINST 70 80 90 100 110 120 ITPNMTFTKT SQKFGQWADS RANTVFGLGF SSEQQLTKFA EKFQEVKEAA KIAKDKTQEK 130 140 150 160 170 180 IETSSNHSQA SSVNGTDDEK ASHAGPANTH LKSENDKLKI ALTQSAANVK KWEIELQTLR 190 200 210 220 230 240 ESNARLTTAL QESAASVEQW KRQFSICRDE NDRLRNKIDE LEEQCSEINR EKEKNTQLKR 250 260 270 280 290 300 RIEELEAELR EKETELKDLR KQSEIIPQLM SECEYVSEKL EAAERDNQNL EDKVRSLKTD 310 320 330 340 IEESKYRQRH LKVELKSFLE VLDGKIDDLH DFRRGLSKLG TDN
	Isoform 3 (2d). Checksum: 29ED437D728A8C59	FASTA	182	20,095
	10 20 30 40 50 60 MGEQPIFTTR AHVFQIDPNT KKNWMPASKQ AVTVSYFYDV TRNSYRIISV DGAKVIINST 70 80 90 100 110 120 ITPNMTFTKT SQKFGQWADS RANTVFGLGF SSEQQLTKFA EKFQEVKEAA KIAKDKTQEK 130 140 150 160 170 180 IETSSNHSQE SGRETPSSTQ ASSVNGTDDE KASHAGPANT HLKSENDKLK IALTQSAANV KK
	Isoform 4 (2c). Checksum: 2B21040C121FD0E3	FASTA	171	18,935
	10 20 30 40 50 60 MGEQPIFTTR AHVFQIDPNT KKNWMPASKQ AVTVSYFYDV TRNSYRIISV DGAKVIINST 70 80 90 100 110 120 ITPNMTFTKT SQKFGQWADS RANTVFGLGF SSEQQLTKFA EKFQEVKEAA KIAKDKTQEK 130 140 150 160 170 IETSSNHSQA SSVNGTDDEK ASHAGPANTH LKSENDKLKI ALTQSAANVK K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Homer regulates the association of group 1 metabotropic glutamate receptors with multivalent complexes of homer-related, synaptic proteins." Xiao B., Tu J.C., Petralia R.S., Yuan J.P., Doan A., Breder C.D., Ruggiero A., Lanahan A.A., Wenthold R.J., Worley P.F. Neuron 21:707-716(1998) [PubMed: 9808458] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Frontal cortex.
[2]	"Molecular characterisation of two structurally distinct groups of human homers, generated by extensive alternative splicing." Soloviev M., Ciruela F., Chan W.-Y., McIlhinney R.A.J. J. Mol. Biol. 295:1185-1200(2000) [PubMed: 10653696] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4).
[3]	"Mammalian neuralized interacts with a novel protein which has a EVH1-like domain." Yoshida M., Ueno M., Nakamura H., Saya H. Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Brain.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Lung.
[5]	"Homer binds a novel proline-rich motif and links group 1 metabotropic glutamate receptors with IP3 receptors." Tu J.C., Xiao B., Yuan J.P., Lanahan A.A., Leoffert K., Li M., Linden D.J., Worley P.F. Neuron 21:717-726(1998) [PubMed: 9808459] [Abstract] Cited for: INTERACTION WITH GRM1; GRM5; DYN3 AND ITPR1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF093263 mRNA. Translation: AAC71027.1. AF093264 mRNA. Translation: AAC71028.1. Y19025 mRNA. Translation: CAB75537.1. Y19026 mRNA. Translation: CAB75538.1. Y19027 mRNA. Translation: CAB75539.1. Y19028 mRNA. Translation: CAB75540.1. AF081530 mRNA. Translation: AAD13748.1. BC012109 mRNA. Translation: AAH12109.1.
IPI	IPI00394745. IPI00394746. IPI00394747. IPI00394748.
RefSeq	NP_004830.2. NP_955362.1. NP_955363.2. NP_955364.2.
UniGene	Hs.578443
3D structure databases
HSSP	HSSP built from PDB template 1I7A based on UniProtKB Q9QWW1.
SMR	Q9NSB8. Positions 1-131.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NSB8. 1 interaction.
Proteomic databases
PRIDE	Q9NSB8.
Genome annotation databases
Ensembl	ENSG00000103942. Homo sapiens. [Contig view]
GeneID	9455.
KEGG	hsa:9455.
NMPDR	fig|9606.3.peg.11138.
Organism-specific databases
GeneCards	GC15M081314.
H-InvDB	HIX0012514.
HGNC	HGNC:17513. HOMER2.
MIM	604799. gene.
PharmGKB	PA134870500.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9NSB8.
HOVERGEN	Q9NSB8.
Gene expression databases
ArrayExpress	Q9NSB8.
Bgee	Q9NSB8.
CleanEx	HS_HOMER2.
GermOnline	ENSG00000103942. Homo sapiens.
Family and domain databases
InterPro	IPR000697. EVH1. IPR011993. PH_type. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
Pfam	PF00568. WH1. 1 hit. [Graphical view]
SMART	SM00461. WH1. 1 hit. [Graphical view]
PROSITE	PS50229. WH1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	35424.
SOURCE	Search...

Entry information

Entry name

HOME2_HUMAN

Accession

Primary (citable) accession number: Q9NSB8
Secondary accession number(s): O95269 , O95349, Q9NSB6, Q9NSB7, Q9UNT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)