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Reviewed, UniProtKB/Swiss-Prot Q9NSB8 (HOME2_HUMAN)

Last modified June 16, 2009. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Homer protein homolog 2
      Short name=Homer-2
Alternative name(s):
    Cupidin
Gene names
Name: HOMER2
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length354 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses.

Subunit structure

Isoform 1 and isoform 2 encode coiled-coil structures that mediate homo- and heteromultimerization.

Subcellular location

Cytoplasm. Cell junctionsynapsepostsynaptic cell membranepostsynaptic density. Cell junctionsynapse. Note: Postsynaptic density of neuronal cells. The stabilization and clustering of the metabotropic glutamate receptors appears to be mediated by isoform 1 and isoform 2 at the cell surface.

Domain

The WH1 domain interacts with the PPXXF motif in GRM1, GRM5, RYR1, RYR2, ITPR1, SHANK 1 and SHANK3.

Sequence similarities

Belongs to the Homer family.

Contains 1 WH1 domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

APPP05067-81EBI-2126733,EBI-302661

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9NSB8-1)

Also known as: 2b;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9NSB8-2)

Also known as: 2a;

The sequence of this isoform differs from the canonical sequence as follows:
     130-140: Missing.
Isoform 3 (identifier: Q9NSB8-3)

Also known as: 2d;

The sequence of this isoform differs from the canonical sequence as follows:
     183-354: Missing.
Isoform 4 (identifier: Q9NSB8-4)

Also known as: 2c;

The sequence of this isoform differs from the canonical sequence as follows:
     130-140: Missing.
     183-354: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 354354Homer protein homolog 2
PRO_0000191008

Regions

Domain1 – 110110WH1
Coiled coil92 – 12231 Potential
Coiled coil160 – 329170 Potential

Natural variations

Alternative sequence130 – 14011Missing in isoform 2 and isoform 4.
VSP_009069
Alternative sequence183 – 354172Missing in isoform 3 and isoform 4.
VSP_009070
Natural variant2191R → H: dbSNP rs7175005.
VAR_053366
Natural variant2391I → S: dbSNP rs17158223.
VAR_053367

Experimental info

Sequence conflict1491D → E in AAC71028. Ref.1
Sequence conflict1611H → Q in AAC71028. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (2b) [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 33D258177F798373

FASTA35440,627
        10         20         30         40         50         60 
MGEQPIFTTR AHVFQIDPNT KKNWMPASKQ AVTVSYFYDV TRNSYRIISV DGAKVIINST 

        70         80         90        100        110        120 
ITPNMTFTKT SQKFGQWADS RANTVFGLGF SSEQQLTKFA EKFQEVKEAA KIAKDKTQEK 

       130        140        150        160        170        180 
IETSSNHSQE SGRETPSSTQ ASSVNGTDDE KASHAGPANT HLKSENDKLK IALTQSAANV 

       190        200        210        220        230        240 
KKWEIELQTL RESNARLTTA LQESAASVEQ WKRQFSICRD ENDRLRNKID ELEEQCSEIN 

       250        260        270        280        290        300 
REKEKNTQLK RRIEELEAEL REKETELKDL RKQSEIIPQL MSECEYVSEK LEAAERDNQN 

       310        320        330        340        350 
LEDKVRSLKT DIEESKYRQR HLKVELKSFL EVLDGKIDDL HDFRRGLSKL GTDN 

« Hide

Isoform 2 (2a).

Checksum: 2980EBF0280F1772
Show »

FASTA34339,467
Isoform 3 (2d).

Checksum: 29ED437D728A8C59
Show »

FASTA18220,095
Isoform 4 (2c).

Checksum: 2B21040C121FD0E3
Show »

FASTA17118,935

References

« Hide 'large scale' references
[1]"Homer regulates the association of group 1 metabotropic glutamate receptors with multivalent complexes of homer-related, synaptic proteins."
Xiao B., Tu J.C., Petralia R.S., Yuan J.P., Doan A., Breder C.D., Ruggiero A., Lanahan A.A., Wenthold R.J., Worley P.F.
Neuron 21:707-716(1998) [PubMed: 9808458] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Tissue: Frontal cortex.
[2]"Molecular characterisation of two structurally distinct groups of human homers, generated by extensive alternative splicing."
Soloviev M., Ciruela F., Chan W.-Y., McIlhinney R.A.J.
J. Mol. Biol. 295:1185-1200(2000) [PubMed: 10653696] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4).
[3]"Mammalian neuralized interacts with a novel protein which has a EVH1-like domain."
Yoshida M., Ueno M., Nakamura H., Saya H.
Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Brain.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Lung.
[5]"Homer binds a novel proline-rich motif and links group 1 metabotropic glutamate receptors with IP3 receptors."
Tu J.C., Xiao B., Yuan J.P., Lanahan A.A., Leoffert K., Li M., Linden D.J., Worley P.F.
Neuron 21:717-726(1998) [PubMed: 9808459] [Abstract]
Cited for: INTERACTION WITH GRM1; GRM5; DYN3 AND ITPR1.
+Additional computationally mapped references.

Cross-references

Sequence databases

AF093263 mRNA. Translation: AAC71027.1.
AF093264 mRNA. Translation: AAC71028.1.
Y19025 mRNA. Translation: CAB75537.1.
Y19026 mRNA. Translation: CAB75538.1.
Y19027 mRNA. Translation: CAB75539.1.
Y19028 mRNA. Translation: CAB75540.1.
AF081530 mRNA. Translation: AAD13748.1.
BC012109 mRNA. Translation: AAH12109.1.
IPIIPI00394745.
IPI00394746.
IPI00394747.
IPI00394748.
RefSeqNP_004830.2.
NP_955362.1.
NP_955363.2.
NP_955364.2.
UniGeneHs.578443

3D structure databases

HSSPHSSP built from PDB template 1I7A based on UniProtKB Q9QWW1.
SMRQ9NSB8. Positions 1-131.
ModBaseSearch...

Protein-protein interaction databases

IntActQ9NSB8. 1 interaction.

Proteomic databases

PRIDEQ9NSB8.

Genome annotation databases

EnsemblENSG00000103942. Homo sapiens. [Contig view]
GeneID9455.
KEGGhsa:9455.
NMPDRfig|9606.3.peg.11138.

Organism-specific databases

GeneCardsGC15M081314.
H-InvDBHIX0012514.
HGNCHGNC:17513. HOMER2.
MIM604799. gene.
PharmGKBPA134870500.
GenAtlasSearch...

Phylogenomic databases

HOGENOMQ9NSB8.
HOVERGENQ9NSB8.

Gene expression databases

ArrayExpressQ9NSB8.
BgeeQ9NSB8.
CleanExHS_HOMER2.
GermOnlineENSG00000103942. Homo sapiens.

Family and domain databases

InterProIPR000697. EVH1.
IPR011993. PH_type.
[Graphical view]
Gene3DG3DSA:2.30.29.30. PH_type. 1 hit.
PfamPF00568. WH1. 1 hit.
[Graphical view]
SMARTSM00461. WH1. 1 hit.
[Graphical view]
PROSITEPS50229. WH1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio35424.
SOURCESearch...

Entry information

Entry nameHOME2_HUMAN
AccessionPrimary (citable) accession number: Q9NSB8
Secondary accession number(s): O95269 expand/collapse secondary AC list , O95349, Q9NSB6, Q9NSB7, Q9UNT7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: October 1, 2000
Last modified: June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents