Q9NSA3 (CNBP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-catenin-interacting protein 1

Alternative name(s):
Inhibitor of beta-catenin and Tcf-4

Gene names

Name:	CTNNBIP1
Synonyms:	ICAT

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	81 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Prevents the interaction between CTNNB1 and TCF family members, and acts as negative regulator of the Wnt signaling pathway. Ref.3
Subunit structure	Binds CTNNB1.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the CTNNBIP1 family.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Cytoplasm Nucleus
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW anterior/posterior pattern specification Inferred from Biological aspect of Ancestor. Source: RefGenome branching involved in ureteric bud morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of Wnt receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of mesenchymal cell proliferation Inferred from mutant phenotype. Source: BHF-UCL negative regulation of protein binding Inferred from direct assay Ref.4. Source: BHF-UCL negative regulation of sequence-specific DNA binding transcription factor activity Inferred from mutant phenotype. Source: BHF-UCL negative regulation of smooth muscle cell proliferation Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of transcription initiation from RNA polymerase II promoter Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of monocyte differentiation Inferred from mutant phenotype. Source: BHF-UCL positive regulation of osteoblast differentiation Inferred from mutant phenotype. Source: BHF-UCL regulation of vascular permeability involved in acute inflammatory response Inferred from mutant phenotype. Source: BHF-UCL
Cellular component	Axin-APC-beta-catenin-GSK3B complex Inferred from direct assay. Source: BHF-UCL cytosol Inferred from direct assay. Source: BHF-UCL nucleus Inferred from direct assay. Source: BHF-UCL
Molecular function	armadillo repeat domain binding Inferred from physical interaction Ref.4. Source: BHF-UCL beta-catenin binding Inferred from physical interaction Ref.4. Source: BHF-UCL
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Ctnnb1	Q02248	7	EBI-747082,EBI-397872	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 81

81

Beta-catenin-interacting protein 1

PRO_0000152882

Secondary structure

1

.

.

.

.

.

.

.

81

Helix Strand Turn

Details...

Helix

11 – 28

18

Helix

35 – 43

9

Helix

45 – 52

8

Sequences

	Sequence		Length	Mass (Da)
	Q9NSA3 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 3DA6CF90B50CE942	FASTA	81	9,170
	10 20 30 40 50 60 MNREGAPGKS PEEMYIQQKV RVLLMLRKMG SNLTASEEEF LRTYAGVVNS QLSQLPPHSI 70 80 DQGAEDVVMA FSRSETEDRR Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Inhibition of Wnt signaling by ICAT, a novel beta-catenin-interacting protein." Tago K., Nakamura T., Nishita M., Hyodo J., Nagai S., Murata Y., Adachi S., Ohwada S., Morishita Y., Shibuya H., Akiyama T. Genes Dev. 14:1741-1749(2000) [PubMed: 10898789] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[3]	"The crystal structure of the beta-catenin/ICAT complex reveals the inhibitory mechanism of ICAT." Graham T.A., Clements W.K., Kimelman D., Xu W. Mol. Cell 10:563-571(2002) [PubMed: 12408824] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH CTNNB1, FUNCTION.
[4]	"ICAT inhibits beta-catenin binding to Tcf/Lef-family transcription factors and the general coactivator p300 using independent structural modules." Daniels D.L., Weis W.I. Mol. Cell 10:573-584(2002) [PubMed: 12408825] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) IN COMPLEX WITH CTNNB1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB021262 mRNA. Translation: BAB03458.1.
BC014300 mRNA. Translation: AAH14300.1.

IPI

IPI00025001.

RefSeq

NP_001012329.1. NM_001012329.1.
NP_064633.1. NM_020248.2.

UniGene

Hs.463759.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LUJ	X-ray	2.50	B	1-75	[»]
1M1E	X-ray	2.10	B	1-81	[»]
1T08	X-ray	2.10	B	8-53	[»]

ProteinModelPortal

Q9NSA3.

SMR

Q9NSA3. Positions 8-53.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9NSA3. 5 interactions.

MINT

MINT-249497.

STRING

Q9NSA3.

PTM databases

PhosphoSite

Q9NSA3.

Polymorphism databases

DMDM

29428025.

Proteomic databases

PeptideAtlas

Q9NSA3.

PRIDE

Q9NSA3.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000361853; ENSP00000354792; ENSG00000178585.
ENST00000377256; ENSP00000366466; ENSG00000178585.
ENST00000377258; ENSP00000366468; ENSG00000178585.
ENST00000377263; ENSP00000366474; ENSG00000178585.
ENST00000400904; ENSP00000383696; ENSG00000178585.

GeneID

56998.

KEGG

hsa:56998.

UCSC

uc001aqk.1. human.

Organism-specific databases

CTD

56998.

GeneCards

GC01M009908.

H-InvDB

HIX0000110.

HGNC

HGNC:16913. CTNNBIP1.

HPA

CAB024893.

MIM

607758. gene.

neXtProt

NX_Q9NSA3.

GenAtlas

Search...

Phylogenomic databases

GeneTree

ENSGT00510000048990.

HOVERGEN

HBG051037.

InParanoid

Q9NSA3.

OrthoDB

EOG4GB77S.

Gene expression databases

ArrayExpress

Q9NSA3.

Bgee

Q9NSA3.

CleanEx

HS_CTNNBIP1.

Genevestigator

Q9NSA3.

GermOnline

ENSG00000178585. Homo sapiens.

Family and domain databases

InterPro

IPR009428. ICAT.
[Graphical view]

Gene3D

G3DSA:1.10.10.490. ICAT. 1 hit.

KO

K04493.

Pfam

PF06384. ICAT. 1 hit.
[Graphical view]

SUPFAM

SSF81730. ICAT. 1 hit.

ProtoNet

Search...

Other

NextBio

62715.

SOURCE

Search...

Entry information

Entry name

CNBP1_HUMAN

Accession

Primary (citable) accession number: Q9NSA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.